Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases
(A, B) Total RNA was extracted from unreactivated or reactivated KSHV-positive TREX-BCBL-1 (A) cells or iSLK.219 cells (B) and subjected to RT-qPCR to measure endogenous levels of the GADD45B or GAPDH transcript. (C) 293T cells were transfected with an empty vector or a plasmid expressing SOX or vhs. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure endogenous levels of GADD45B or GAPDH transcripts. (D) Diagram of the reporter constructs where fragments of GADD45B transcript were fused to GFP. (E) 293T cells were transfected with the indicated GFP-GADD45B fusion constructs in the presence or absence of a plasmid expressing SOX. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure GFP levels.