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Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases

Fig 2

The IL-6-SRE does not protect against cellular endonucleases.

(A) 293T cells were transfected with the indicated dsRed2 reporters. After 24 h, total RNA was harvested and subjected RT-qPCR to measure dsRed mRNA levels. (B) 293T cells were treated with siRNAs targeting Smg6 or control non-targeting siRNAs. 48h later, cells were transfected with the indicated dsRed reporters. After 24h, total RNA was harvested and subjected RT-qPCR to measure dsRed mRNA levels (left). The efficiency of SMG6 knockdown was measured by western blotting, with actin serving as a loading control (right). (C) 293T cells were transfected with the indicated dsRed2 reporters. After 24 h, total RNA was harvested and subjected to RT-qPCR to measure dsRed mRNA levels. (D) 293T cells were transfected with an empty vector (mock) or a plasmid expressing SCoV nsp1 along with the indicated GFP reporters. After 24 h, total RNA was harvested and subjected RT-qPCR to measure GFP mRNA levels.

Fig 2

doi: https://doi.org/10.1371/journal.ppat.1006593.g002