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Identification of Interactions between Sindbis Virus Capsid Protein and Cytoplasmic vRNA as Novel Virulence Determinants

Fig 2

Analysis and mutation of the SINV C:R interaction sites.

A) Magnified regions of the viral genomic RNA with special emphasis on the individual C:R interaction sites prioritized during this study. Plotted on the left y-axis are the Z-scores previously reported in Fig 1. The right y-axis indicates the prevalence of single nucleotide polymorphisms (SNPS) across a curated set of SINV genomic RNAs; higher values indicate increased base identity variation at a particular nucleotide but are not informative as to relative base conservation. Nucleotide position is reported on the x-axis. B) The individual nucleotide sequences of the C:R interaction sites are described in regards to parental sequence (top) and mutant sequence (bottom). The mutated nucleotides are highlighted in red. C) Quantitative analysis of the individual C:R interaction sites following mutation indicates that Capsid:RNA binding is significantly reduced relative to parental virus. Data shown is the mean of three independent biological replicates, with the error bar representing the standard deviation of the mean.

Fig 2