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The host ubiquitin-dependent segregase VCP/p97 is required for the onset of human cytomegalovirus replication

Fig 8

Altered MIE splicing is not due to changes in cell cycle following VCP knockdown.

Fibroblast cells were transfected with negative control siRNA or VCP siRNA and cells were fixed and stained with propidium iodide 4 days post transfection. Untransfected cells were treated with 0.5 μg/ml nocodazole as a positive control of G2/M arrest. DNA content was then measured by FACS analysis and defined as G1, S or G2 using FlowJo software.

Fig 8