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The host ubiquitin-dependent segregase VCP/p97 is required for the onset of human cytomegalovirus replication

Fig 3

Knockdown of VCP results in a specific loss of the major immediate early protein IE2.

(A) Fluorescence microscopy demonstrates that all cells are infected with HCMV at 48 HPI following transfection with VCP siRNA, indicating no major effect on virus entry and translocation to the nucleus. (B) Western blot analysis for viral proteins of each major kinetic class shows a clear loss of IE2 expression and downstream early and late viral proteins.

Fig 3