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Magnaporthe oryzae Glycine-Rich Secretion Protein, Rbf1 Critically Participates in Pathogenicity through the Focal Formation of the Biotrophic Interfacial Complex

Fig 5

The RBF1-disrupted blast fungus infects rice plants with impaired immunity.

(A) Symptoms on the spot-inoculated rice leaf blades at 5 dpi (upper) and the GUS staining (lower). Both the WT and Δrbf1-1 (KO) were transformed with TEFp::GUS to visualize invasive hyphae. NT, non-transgenic rice; +ABA, inoculated with 30 μM abscisic acid; NahG, transgenic rice expressing the salicylate hydroxylase gene. Bar = 5 mm. (B) Proliferation of M. oryzae in leaf blades at 6 dpi evaluated by quantitative PCR. DNA amount of M. oryzae 28SrDNA (Mo28S) relative to rice eEF-1α (OsEF1) in spot-inoculated leaf fragments were measured. Data are represented as mean values ± SE (n = 7 plants for NT and +ABA, and n = 5 plants for NahG samples). Different letters above bars indicate significant differences at P < 0.05 (Student’s t-test of paired comparison).

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1005921.g005