Magnaporthe oryzae Glycine-Rich Secretion Protein, Rbf1 Critically Participates in Pathogenicity through the Focal Formation of the Biotrophic Interfacial Complex
(A) Co-localization of Rbf1:mCherry with Pwl2:GFP at the BIC. Rice leaf sheaths were inoculated with M. oryzae transformed with RBF1p::RBF1:mCherry and PWL2p::PWL2:GFP, and observed using a confocal microscope at 36 hpi. DIC, differential interference contrast image. (B) Accumulation of Rbf1:mCherry in the rice cytoplasm. Rice leaf sheaths were inoculated with M. oryzae transformed with RBF1p::RBF1:mCherry and IH at 36 hpi were observed after sucrose-induced plasmolysis. Confocal mCherry images were merged with DIC images. Data obtained using a transformant with PWL2p::PWL2:mCherry is shown as the control. (C) Accumulation of the Rbf1:mCherry fused with a nuclear localization signal (NLS) in the host nucleus. Rice leaf sheaths infected by the transformant containing RBF1p::RBF1:mCherry:NLS (upper) were observed using a confocal microscope at 24 hpi. Arrows indicate rice nuclei with mCherry signals. The transformant containing PWL2p::PWL2:mCherry:NLS (lower) is shown as the control. Asterisks indicate appressoria. Bar = 10 μm.