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Inactivation of Prions and Amyloid Seeds with Hypochlorous Acid

Fig 5

Effect of disinfectant exposure time on inactivation of hamster scrapie seeding activity on wires coated with ScBH.

Stainless steel wire segments (3–4mm) pre-coated with hamster ScBH at a 10−3 tissue dilution were submersed in (A) saline (mock disinfectant), (B) BrioHOCl, (C) 1 N NaOH or (D) 40% bleach (2.4% hypochlorite) for 0.5–60 min prior to a quick rinse and RT-QuIC analysis using hamster (90–231) rPrPC substrate. Each trace represents the average normalized ThT fluorescence of 4 replicate wells.

Fig 5