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Inactivation of Prions and Amyloid Seeds with Hypochlorous Acid

Fig 3

Effects of bleach, NaOH and Environ LpH on hamster scrapie seeding activity.

ScBH was pretreated for 1 h in (A) saline (mock disinfectant) (red), (B) 40% bleach (2.4% hypochlorite) (green), (C) 1 N NaOH (black) or (D) 2% Environ LpH (purple) at a ratio (v/v) of 100:1 disinfectant to 10% ScBH. Similar disinfectant pretreatments of NBH are indicated in gray. Resulting samples were then subjected to serial 10-fold dilutions and RT-QuIC analysis was performed with hamster (90–231) rPrPC substrate using the designated tissue dilutions as seeds. Each trace represents the average normalized ThT fluorescence of 4 replicate wells.

Fig 3