Transformed Recombinant Enrichment Profiling Rapidly Identifies HMW1 as an Intracellular Invasion Locus in Haemophilus influenzae
(A) RdS NovR. (B) RdS NalR. The x-axes indicate recipient genome coordinate, and the y-axes indicate the percent donor alleles. Blue dots and grey lines indicate donor-specific SNPs. Salmon color shows the limits of detection, or 1/depth (alignments with mapping quality = 0 were ignored, which excludes multiply mapping reads). Pools are indicated as Pn. The two wide views at the top of A and B show the genome-wide donor allele frequencies for Pool 0 and Pool 8, whereas the lower four zoom around the nearly fixed locus selected for by serial gentamicin protection assay (indicated by purple circles) for Pools 0, 2, 4, and 8.