Candida glabrata Binding to Candida albicans Hyphae Enables Its Development in Oropharyngeal Candidiasis
Fig 3
C. albicans Als3 and Als1 hyphal cell wall adhesins are involved in C. glabrata adherence to C. albicans hyphae.
(A) C. glabrata (CgVSY55) adherence to C. albicans Als1, Als3 and Als1/3 deficient strains was measured using fluorescent microscopy. C. glabrata showed a significant (**P<0.001,***P<0.0006) decrease in adherence to C. albicans hyphae of als3Δ/Δ, als1Δ/Δ, and als1/als3Δ/Δ mutants; while C. albicans Als1 and Als3 complementation strains had restored adherence. (B) S. cerevisiae strains expressing C. albicans Als1 and Als3 adhesins and an empty vector (control) were incubated with CgVSY55 and their adherence was quantifed by direct visualization. Both Als1 and Als3 expressing S. cerevisiae strains had a significant (***P<0.001) increase in Binding Index compared to control empty vector. Differences between groups were analyzed by a student’s t test.