De novo GTP Biosynthesis Is Critical for Virulence of the Fungal Pathogen Cryptococcus neoformans
(A) Resistance to the IMPDH inhibitor MPA was assessed in all haploid molecular types of the pathogenic C. neoformans/C. gattii species complex using a serial dilution spotting assay on YNB plus 5 µg/mL MPA. (B) Precise sensitivity to MPA was determined via MIC broth microdilution assay. MIC50 and MIC90 are the concentrations at which growth was inhibited by 50% or 90%, respectively. Standard error bars are displayed for all strains.