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Large-Scale Gene Disruption in Magnaporthe oryzae Identifies MC69, a Secreted Protein Required for Infection by Monocot and Dicot Fungal Pathogens

Figure 5

Secretion of MC69(C36A)::mCherry and MC69::mCherry fusion proteins.

(A) Schematic diagram of MC69(36A)::mCherry fusion protein expression construct. (B) Merged DIC and mCherry images of rice leaf sheath cells infected by the MC69(C36A)::mCherry-expressing transformants 24 h and 48 h after inoculation. Scale bar = 20 ┬Ám. (C) Western blot probed with an anti-DsRed antibody. Samples were loaded as follows: lane 1, culture filtrate from mCherry-expressing strain; lane 2, culture filtrate from MC69::mCherry-expressing strain; lane 3, culture filtrate from MC69(C36A)::mCherry-expressing strain.

Figure 5