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Large-Scale Gene Disruption in Magnaporthe oryzae Identifies MC69, a Secreted Protein Required for Infection by Monocot and Dicot Fungal Pathogens

Figure 2

Microscopic analysis suggests that MC69 promoter is constitutively active and MC69::mCherry fusion protein is secreted.

(A) and (D) Schematic diagrams of mCherry and MC69::mCherry fusion protein expression constructs. (B) and (E) Conidia from WT+mCherry and mc69+MC69::mCherry were harvested, and appressorium development was observed for 12 h on glass coverslips. Merged DIC and mCherry (red) images were taken. Scale bars = 10 µm. (C) and (F) Merged DIC and mCherry images of the rice leaf sheath cells infected with mCherry- and MC69::mCherry-expressing transformants 24 h and 48 h after inoculation. Scale bars = 20 µm. (G) Blast symptoms caused by Ina72 WT, mc69 and mc69+MC69::Cherrry on rice (cv. Shin No. 2) 7 days after inoculation.

Figure 2