Structural and Functional Studies on the Interaction of GspC and GspD in the Type II Secretion System
Localization of chromosomally expressed GFP-GspC was examined in wild-type and gspD mutant backgrounds by fluorescence microscopy. GFP-GspC displayed a continuous membrane localization in the gfp-gspC gspD– strain (second panel) compared to the wild-type background (first panel). Punctate fluorescence was restored when the gfp-gspC gspD- strain was complemented with GspD on a plasmid (third panel). Expression of GspDI18R/N22Y in the gfp-gspC gspD– strain resulted in membrane localization similar the pMMB vector control (fourth panel).