Isolation and Characterization of Adenoviruses Persistently Shed from the Gastrointestinal Tract of Non-Human Primates
A Simplot analysis of New Iberia species B adenoviral isolates aligned with a schematic annotated genomic structure of the SAdV-27.1 showing evidence of an incidence of recombination between viral isolates from the same compound. The analysis demonstrates homology on the left genomic end extending up to the DNA Binding Protein (DBP) open reading frame with various species B isolates from within one subspecies (represented by SAdV-32) from the New Iberia chimpanzee colony, followed by an 100K, 22K, 33K and partial E3 gene region of near complete identity to the structurally distinct New Iberia isolate SAdV-35.1 of a distinct subspecies within species B. To the right of this region of identity the homology transitions to the fiber region first to B isolate SAdV-33 and subsequently to SAdV-32 again. Further evidence is provided by SAdV-27.2, isolated from a gorilla in a different geographical location (Atlanta zoo). This genome is closely related to SAdV-27.1 and is almost identical in hexon but did not undergo the presumed recombination event between DBP and E3. The left and extreme right ends of the SADV-27.1 genome demonstrate close but not complete identity to SAdV-32 indicating a close relationship to the putative parental adenovirus of this event of homoplasy. The left junction of the recombination event is a clearly defined junction centrally in the DNA binding protein as indicated by the dotted blue line. The right end junction was less well-defined due to a region of lack of complete identity in the fiber-proximal part of the coding region of the E3 gene family. This region is indicated by the dotted blue lines in the sequence. The more distantly related species B isolate SAdV-35, species C isolate SAdV-31 and species E isolate SAdV-38 (all isolated from the New Iberia chimpanzee colony) are provided in the analysis as reference.