The multifaceted role of the viral 2A protease in enterovirus replication and antagonism of host antiviral responses
Fig 5
Distinct effects of CVB3-2Awt and CVB3-2Amut on cell morphology and death.
(A) HeLa-R19 cells were infected with 3 CDcs-2Awt and 3 CDcs-2Amut at an MOI of 50, fixed at 6, 8, 10, 12 and 16 hpi and, and analyzed by IF analysis. Nuclei were stained with DAPI, dsRNA was detected as marker for infection, and β-tubulin was used as a cytoskeleton marker. Fragmented nuclei are indicated by white triangles. Scale bar: 20 μm. (B) Quantification of infected cells displaying nuclear fragmentation in panel (A).