Entamoeba histolytica activation of caspase-1 degrades cullin that attenuates NF-κB dependent signaling from macrophages
Fig 2
Cullin-1/5 degradation requires Gal-lectin-mediated contact with macrophage.
(A, B) THP-1 macrophages were stimulated with either Eh (10:1 ratio) alone or with Eh preincubated with 55mM Galactose or Glucose for 10 min at room temperature and the degradation of the cullin-1/5 determined. (C, D) THP-1 macrophages were stimulated with native Eh Gal-lectin (500ng/ml) for 1h and 2h. Post incubation, cells were washed and lysed. Equal amount of protein was loaded onto 7.5% SDS-PAGE gel and immunoblotted with anti-cullin-1, anti-cullin-5 and anti-GAPDH antibody. Highlighted boxed areas on the figures show point of interest for cullin-1/5 as described in text. Data are representative of three independent experiments and statistical significance was carried out with Students t- test. Bar represent mean ± SEM. **P<0.01, ***P<0.001.