pMGF505-7R determines pathogenicity of African swine fever virus infection by inhibiting IL-1β and type I IFN production
Fig 5
ASFV-Δ7R induces higher type I IFN and IL-1β production compared with ASFV-WT.
(A-D) PAMs were either mock-infected or infected with ASFV HLJ/18 (ASFV-WT) or ASFV-Δ7R at an MOI of 0.5. At 24 or 48 hpi, the IL-1β (A) and IFN-β (B) levels in the cell culture supernatants were detected by ELISA and the mRNA levels in the cell lysates were determined by qPCR. The genomic copy numbers of ASFV in PAMs were measured by qPCR (C) and the cell viabilities were analyzed using a CCK-8 counting kit (D). A p value of less than 0.05 was considered statistically significant. *p<0.05, **p<0.01, ***p<0.001.