Long non-coding RNA KIKAT/LINC01061 as a novel epigenetic regulator that relocates KDM4A on chromatin and modulates viral reactivation
Fig 5
Identification of AMOT as a potential KDM4A-KIKAT/LINC01061 target (A) Histograms of ChIP-seq profiles for KDM4A binding at AMOT loci in SLK-VC and SLK-KIKAT/LINC01061 cells. Red arrow in the enlarged view shows the direction of KDM4A relocation. (B) ChIP-qPCR assay revealed the binding of KDM4A (upper panel) and the modification of H3K4me3 (middle panel) and H3K9me3 (lower panel) to the promoter of AMOT in SLK-VC and SLK-KIKAT/LINC01061 cells. (C and D) RT-qPCR analysis verification of AMOT expression in transient KIKAT/LINC01061 transduced SLK cells (C) and in SLK-KIKAT/LINC01061 cell lines (D). (E) RT-qPCR analysis revealed the expression of KIKAT/LINC01061 and AMOT in SLK cells after transfected with siRNA targeting KIKAT/LINC01061 and with control siGLO. (F) ChIP-qPCR assay revealed the binding of KDM4A to the promoter of AMOT in SLK cells transient transfected with siKIKAT/LINC01061. (G) RT-qPCR analysis revealed the expression of AMOT in SLK and SLK-KDM4A KO cells. (H) ChIP-qPCR assay revealed the modification of H3K9me3 on the AMOT promoter in SLK-KDM4A KO cells. Significance was determined by student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. non-significant.