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A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization

Fig 5

Mg1LysM mutants are impaired in protection against chitinases.

(A) Trichoderma viride incubated with Mg1LysM with the amine-reactive fluorescent dye BODIPY, or with BODIPY only as a control, for 4 hours and bright field and fluorescence microscopy pictures are shown. (B) Microscopic pictures of Fusarium oxysporum f. sp. lycopersici grown in vitro in the absence or presence of wild-type or mutant Mg1LysM, 4 hours after addition of tomato hydrolytic enzymes (HE) that include chitinases, or water as control.

Fig 5

doi: https://doi.org/10.1371/journal.ppat.1008652.g005