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A secreted LysM effector protects fungal hyphae through chitin-dependent homodimer polymerization

Fig 3

Two Mg1LysM protomers bind a single chitin hexamer with high affinity.

Isothermal titration calorimetry of (GlcNAc)₆ binding by wild-type Mg1LysM produced in E. coli, and the mutants T²⁸R, K³¹A and D⁵⁴A. The dissociation constant (K_d) and the stoichiometry (N) of the interactions are indicated.

doi: https://doi.org/10.1371/journal.ppat.1008652.g003