Tenuivirus utilizes its glycoprotein as a helper component to overcome insect midgut barriers for its circulative and propagative transmission
Fig 5
Localizations of RSV virions, NSvc2-N and NSvc2-C in SBPH midgut epithelial cells.
(A and B) RSV virions were detected in the early endosomes inside midgut epithelial cells by labeling using the Rab5 antibody (A) or in the late endosomes by labeling using the Rab7 antibody (B). Localization of RSV virions in the Rab5 antibody-labeled early endosomes or in the Rab7 antibody-labeled late endosomes are indicated with arrows. Actin antibody was used to visualize actin filaments inside the midgut epithelial cells. Bar, 25 μm. (C and D) NSvc2-N and NSvc2-C were detected inside the early endosomes labeled with the EEA1 antibody. Co-localizations of NSvc2-N or NSvc2-C with EEA1 or actin in different endosomes are indicated with arrows. Bar, 25 μm. (E) RSV virions:NSvc2-N complexes were released from the actin labelled late endosomes into the cytosol of epithelial cells. The white dashed cycles indicate the regions where the RSV virions:NSvc2-N complexes were detected in the cytosol. Bar, 25 μm. (F) RSV virions was released into the cytosol of epithelial cells but not NSvc2-C. The white dashed boxes indicate a region where RSV virions were released from the endosomes while NSvc2-C was still associated with endosomes. Bar, 25 μm. (G) RSV virions were released from the Rab7 antibody-labeled late endosomes into the cytosol of epithelial cells. The released RSV virions are indicated with arrows. ML, midgut lumen; EC, epithelial cells; Bar, 25μm.