Specificity and functional interplay between influenza virus PA-X and NS1 shutoff activity
Fig 6
Validation of RNA-Seq data by qRT-PCR and ELISA.
IFN-λ1, IFN-β1, and IL-6 mRNAs were quantitated by qRT-PCR and compared with RNA-Seq data. Secreted IFN-λ1 and IL-6 in the culture medium were quantified by ELISA at 16 and 24 h pi.