Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques
Fig 7
Lymph node effacement promotes Mtb growth.
A. H&E and Auramine-rhodamine (A-R) staining of a severely-effaced thoracic lymph node (previously depicted in Fig 6C). The location of a large granuloma, indicated by white and grey dashed lines in the H&E and A-R panels, respectively, corresponds with substantial numbers of A-R-stained Mtb. Inset regions (black and white boxes in the H&E and A-R panels, respectively) show the interface between granulomatous and non-granulomatous lymph node regions. B, C. CFU (B) and CEQ (C) of thoracic lymph nodes with ≤50% or >50% effacement (effacement determined by H&E section). D. Mtb killing capacity (CFU/CEQ) of lymph nodes are not affected by the degree of effacement. Each data point is one lymph node. The CFU was transformed by adding 1 to reflect sterile but CEQ+ lymph nodes. Statistical test is Mann-Whitney.