Lymph nodes are sites of prolonged bacterial persistence during Mycobacterium tuberculosis infection in macaques
Fig 6
Histologic and immunohistochemical characterization of Mtb-infected macaque lymph nodes with varying levels of disease.
FFPE tissue sections from Mtb-infected macaques were stained with hematoxylin and eosin (H&E) to show the tissue morphology and immunohistochemistry was performed on serial sections to identify the lymph node’s cellular, vascular, and structural elements. The box in the full-lymph node image indicates the region for the magnified panels (below) A. Lymph node showing no histologic evidence of disease and normal lymph node architecture. B. Lymph node demonstrating histologically-moderate disease where focal granulomas are present in T cell regions but not yet distorting the overall nodal architecture. Arrow indicates a granuloma. C. Severe lymph node disease showing large-scale disruption of the normal nodal structure in the vicinity of large coalescing granulomas. Arrow indicates a granuloma. Black scale bar (lower left) for the full-scale lymph nodes represents 2 mm. White scale bar (lower left, second column) for magnified image fields, represents 100 μm.