Mapping Polyclonal HIV-1 Antibody Responses via Next-Generation Neutralization Fingerprinting
Fig 5
Prediction of potentially novel specificities and confidence scores in NFP analysis with virus panel f61.
(A) Histograms of residual scores for simulated sera with dominant known (green) vs. unknown (red) specificities. (B) Frequency of predicted known antibody signals (colored) for simulated sera with unknown specificities. (C-E) Relationship between serum delineation error (a measure of NFP prediction accuracy) and three confidence scores: (C) residual scores (for values of at least -0.5), (D) median of the ten delineation scores, and (E) frequency of random signals for a set of simulated sera with dominant known antibody specificities (dots). For each of the three confidence scores, higher values were generally associated with greater serum delineation errors. (F) Complementarity of confidence scores. Shown are the frequency of random signals and median of delineation scores for simulated sera (dots) with residual scores less than -0.1.