An Invertebrate Warburg Effect: A Shrimp Virus Achieves Successful Replication by Altering the Host Metabolome via the PI3K-Akt-mTOR Pathway
Figure 5
Pretreatment with Torin 1 inhibited the WSSV-induced Warburg effect at the genome replication stage of WSSV infection (12 hpi) in shrimp hemocytes.
Two hours after treatment with Torin 1, shrimp were injected with PBS or a WSSV inoculum. At 12(10 shrimp per pool) were collected from each group. Changes in the metabolomic levels of the WSSV-infected samples relative to the PBS controls are color-coded as described in Figure 1. Figure S3 shows changes in the metabolome at 24 hpi. Numerical data for 12 hpi and 24 hpi is given in Table S2. Changes in the metabolome for Torin-PBS versus PEG-PBS are shown in Fig. S3, with numerical data given in Table S2.