T-Cell Tropism of Simian Varicella Virus during Primary Infection
Figure 5
Detection of SVV in lymphoid organs from infected African green monkeys.
(A) Real-time qPCR analysis of SVV DNA load in tonsil, lymph nodes and spleen from SVV-wt− (closed squares) and SVV-EGFP− (open squares) infected monkeys at 9, 13 and 20 dpi. Squares indicate individual tissues, i.e., tonsils (red), tracheobronchial lymph nodes (LN) (green), axillary LN (pink), mandibular LN (blue), inguinal LN (orange) and spleen (black). Horizontal bar indicates the median value. (B–D) Serial sections of tonsil from an SVV-wt−infected monkey stained with hematoxylin and eosin (inset shows a Cowdry type A intranuclear inclusion body) (B) or examined immunohistochemically using rabbit anti-SVV antibodies (C) or control normal rabbit serum (D). Magnification: 200×. The area of tonsils containing multiple intranuclear inclusion bodies contained numerous cells expressing SVV protein. **p<0.01 by Mann-Whitney test.