MrkH, a Novel c-di-GMP-Dependent Transcriptional Activator, Controls Klebsiella pneumoniae Biofilm Formation by Regulating Type 3 Fimbriae Expression
Figure 5
Type 3 fimbriae expression by K. pneumoniae AJ218.
(A) Mannose resistant Klebsiella-like hemagglutination (MR/K HA) assays using human erythrocytes. MR/K HA titer is expressed as the lowest concentration (CFU/mL) of bacteria causing a visible agglutination reaction. Values represent the mean of three independent experiments. The error bars represent the standard deviation. Statistical significance between AJ218 wild-type and isogenic mutants was analyzed by one-way ANOVA and Tukey HSD post-hoc comparisons are reported, where *** = P<0.001. (B) Cell lysates were prepared from the indicated strains and analyzed by SDS-PAGE and immunoblotting with anti-MrkA antiserum. The MrkA pilin monomer (which migrates at approximately 21 kDa) is labeled.