Compensatory Evolution of pbp Mutations Restores the Fitness Cost Imposed by β-Lactam Resistance in Streptococcus pneumoniae
Figure 6
Protein localization in wild-type and pbp mutants by fluorescence microscopy.
A) Localization of FtsZ. Microphotographs indicate the most common patterns of FtsZ localization through the cell cycle for wt and pbp mutants. Polyclonal antibody against FtsZ was used for its detection by immunofluorescence microscopy. In the Cp1015 (wt) strain, FtsZ is localized at the equatorial ring and septum, In Cp 1015 pbp2b28, the patterns suggest FtsZ delocalization and cell cycle disruption. The triple pbp mutant showed a wild-type FtsZ placement. B) Localization of PBP2b28-GFP. Microphotographs indicate the most common patterns found for PBP2b localization through the cell cycle in the wt and pbp2b28 mutant. Representative images are shown from experiments that were repeated independently three times (bar scale, 1 µm).