Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

Zachary T. Berndsen; Marjahan Akhtar; Mahima Thapa; Tim J. Vickers; Aaron Schmitz; Jonathan L. Torres; Sabyasachi Baboo; Pardeep Kumar; Nazia Khatoon; Alaullah Sheikh; Melissa Hamrick; Jolene K. Diedrich; Salvador Martinez-Bartolome; Patrick T. Garrett; John R. Yates III; Jackson S. Turner; Renee M. Laird; Frédéric Poly; Chad K. Porter; Jeffrey Copps; Ali H. Ellebedy; Andrew B. Ward; James M. Fleckenstein

doi:10.1371/journal.ppat.1012241

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Peer-reviewed

Research Article

Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

Zachary T. Berndsen,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Resources, Writing – original draft, Writing – review & editing

Current Address: Current affiliation: Department of Biochemistry, University of Missouri Columbia, Columbia, Missouri, United States of America.

Affiliation Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, California, United States of America

https://orcid.org/0000-0003-4531-9907

⨯
Marjahan Akhtar,

Roles Formal analysis, Investigation

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Mahima Thapa,

Roles Investigation

Affiliation Department of Pathology and Immunology, Washington University in Saint Louis, School of Medicine, Saint Louis, Missouri, United States of America
⨯
Tim J. Vickers,

Roles Investigation, Methodology, Writing – original draft, Writing – review & editing

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Aaron Schmitz,

Roles Investigation, Methodology, Writing – original draft

Affiliation Department of Pathology and Immunology, Washington University in Saint Louis, School of Medicine, Saint Louis, Missouri, United States of America
⨯
Jonathan L. Torres,

Roles Investigation

Affiliation Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, California, United States of America
⨯
Sabyasachi Baboo,

Roles Investigation, Methodology

Affiliation Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, United States of America
⨯
Pardeep Kumar,

Roles Conceptualization, Investigation, Methodology

Current Address: Current affiliation: Pfizer, Saint Louis, Missouri, United States of America.

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Nazia Khatoon,

Roles Investigation

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Alaullah Sheikh,

Roles Investigation, Methodology, Writing – original draft

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Melissa Hamrick,

Roles Investigation

Affiliation Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America
⨯
Jolene K. Diedrich,

Roles Investigation

Affiliation Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, United States of America
⨯
Salvador Martinez-Bartolome,

Roles Investigation

Affiliation Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, United States of America
⨯
Patrick T. Garrett,

Roles Investigation

Affiliation Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, United States of America
⨯
John R. Yates III,

Roles Resources, Supervision

Affiliation Department of Molecular Medicine, The Scripps Research Institute, La Jolla, California, United States of America
⨯
Jackson S. Turner,

Roles Investigation, Methodology

Affiliation Department of Pathology and Immunology, Washington University in Saint Louis, School of Medicine, Saint Louis, Missouri, United States of America
⨯
Renee M. Laird,

Roles Investigation

Current Address: Current affiliation: Department of Diarrheal Disease Research, Walter Reed Army Institute of Research, Silver Springs, Maryland, United States of America.

Affiliation Operationally Relevant Infections Department, Naval Medical Research Command (NMRC), Silver Spring, Maryland, United States of America
⨯
Frédéric Poly,

Roles Investigation

Affiliation Operationally Relevant Infections Department, Naval Medical Research Command (NMRC), Silver Spring, Maryland, United States of America
⨯
Chad K. Porter,

Roles Investigation

Affiliation Translational and Clinical Research Department, Naval Medical Research Command (NMRC), Silver Spring, Maryland, United States of America
⨯
Jeffrey Copps,

Roles Investigation

Affiliation Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, California, United States of America
⨯
Ali H. Ellebedy,

Roles Methodology, Project administration, Supervision

Affiliations Department of Pathology and Immunology, Washington University in Saint Louis, School of Medicine, Saint Louis, Missouri, United States of America, Center for Vaccines and Immunity to Microbial Pathogens, Washington University School of Medicine, St Louis, Missouri, United States of America, The Andrew M. and Jane M. Bursky Center for Human Immunology and Immunotherapy Programs, Washington University School of Medicine, St Louis, Missouri, United States of America
⨯
Andrew B. Ward ,

Roles Conceptualization, Project administration, Resources, Supervision, Writing – original draft

* E-mail: andrew@scripps.edu (ABW); jfleckenstein@wustl.edu (JMF)

Affiliation Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, California, United States of America
⨯
[ ... ],
James M. Fleckenstein

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

* E-mail: andrew@scripps.edu (ABW); jfleckenstein@wustl.edu (JMF)

Affiliations Department of Medicine, Division of Infectious Diseases, Washington University in Saint Louis, School of Medicine. Saint Louis, Missouri, United States of America, Operationally Relevant Infections Department, Naval Medical Research Command (NMRC), Silver Spring, Maryland, United States of America, Medicine Service, Infectious Diseases, Veterans Affairs Health Care System, Saint Louis, Missouri, United States of America

https://orcid.org/0000-0002-1148-697X

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Repeat modules and N-linked glycans define structure and antigenicity of a critical enterotoxigenic E. coli adhesin

Zachary T. Berndsen,
Marjahan Akhtar,
Mahima Thapa,
Tim J. Vickers,
Aaron Schmitz,
Jonathan L. Torres,
Sabyasachi Baboo,
Pardeep Kumar,
Nazia Khatoon,
Alaullah Sheikh

Published: September 16, 2024
https://doi.org/10.1371/journal.ppat.1012241

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Glycosylation
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