Rational Development of an Attenuated Recombinant Cyprinid Herpesvirus 3 Vaccine Using Prokaryotic Mutagenesis and In Vivo Bioluminescent Imaging

Maxime Boutier; Maygane Ronsmans; Ping Ouyang; Guillaume Fournier; Anca Reschner; Krzysztof Rakus; Gavin S. Wilkie; Frédéric Farnir; Calixte Bayrou; François Lieffrig; Hong Li; Daniel Desmecht; Andrew J. Davison; Alain Vanderplasschen

doi:10.1371/journal.ppat.1004690

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Open Access

Peer-reviewed

Research Article

Rational Development of an Attenuated Recombinant Cyprinid Herpesvirus 3 Vaccine Using Prokaryotic Mutagenesis and In Vivo Bioluminescent Imaging

Maxime Boutier,

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Maygane Ronsmans,

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Ping Ouyang,

Current address: Key Laboratory of Animal Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Guillaume Fournier,

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Anca Reschner,

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Krzysztof Rakus,

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Gavin S. Wilkie,

Affiliation MRC—University of Glasgow Centre for Virus Research, Glasgow, United Kingdom
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Frédéric Farnir,

Affiliation Biostatistics and Bioinformatics, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Calixte Bayrou,

Affiliation Pathology, Department of Morphology and Pathology, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
François Lieffrig,

Affiliation Fish Pathology Lab, Department of Biotechnology, CER Groupe, Marloie, Belgium
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Hong Li,

Affiliation USDA-ARS-ADRU, Washington State University, Pullman, Pullman, Washington, United States of America
⨯
Daniel Desmecht,

Affiliation Pathology, Department of Morphology and Pathology, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯
Andrew J. Davison,

Affiliation MRC—University of Glasgow Centre for Virus Research, Glasgow, United Kingdom
⨯
Alain Vanderplasschen

* E-mail: A.vdplasschen@ulg.ac.be

Affiliation Immunology-Vaccinology, Department of Infectious and Parasitic Diseases, Fundamental and Applied Research for Animals & Health (FARAH), Faculty of Veterinary Medicine, University of Liège, Liège, Belgium
⨯

Rational Development of an Attenuated Recombinant Cyprinid Herpesvirus 3 Vaccine Using Prokaryotic Mutagenesis and In Vivo Bioluminescent Imaging

Maxime Boutier,
Maygane Ronsmans,
Ping Ouyang,
Guillaume Fournier,
Anca Reschner,
Krzysztof Rakus,
Gavin S. Wilkie,
Frédéric Farnir,
Calixte Bayrou,
François Lieffrig

Published: February 20, 2015
https://doi.org/10.1371/journal.ppat.1004690

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