TY - JOUR T1 - Activation of Shigella flexneri type 3 secretion requires a host-induced conformational change to the translocon pore A1 - Russo, Brian C. A1 - Duncan, Jeffrey K. A1 - Wiscovitch, Alexandra L. A1 - Hachey, Austin C. A1 - Goldberg, Marcia B. Y1 - 2019/11/14 N2 - Author summary The movement of bacterial proteins across membranes is essential for bacterial physiology and bacterial virulence. The type 3 secretion system moves bacterial virulence proteins from the inside of bacterial pathogens into human cells. To do so, the type 3 secretion system forms a pore in the plasma membrane of the target cell, attaches (docks) onto the pore, and delivers virulence proteins through the pore. Docking is essential for establishing a continuous channel from the inside of the bacterium to the inside of the human cell. What enables the type 3 secretion system to dock onto pores is not understood. We show that structural proteins in human cells, intermediate filaments, induce structural rearrangements to the type 3 secretion pore that trigger docking and that enable the subsequent delivery of virulence proteins into human cells. Due to the widespread prevalence of type 3 secretion systems among human pathogens, these findings are likely to broadly enhance our understanding of type 3 secretion. JF - PLOS Pathogens JA - PLOS Pathogens VL - 15 IS - 11 UR - https://doi.org/10.1371/journal.ppat.1007928 SP - e1007928 EP - PB - Public Library of Science M3 - doi:10.1371/journal.ppat.1007928 ER -