About the Authors
- Jakob Begun
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Affiliations Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America , Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
- Jessica M Gaiani
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Affiliation Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, Virginia, United States of America
- Holger Rohde
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Affiliation Institut für Medizinische Mikrobiologie, Virologie und Hygiene, Universitätsklinikum Hamburg-Eppendorf, Hamburg, Germany
- Dietrich Mack
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Affiliation Medical Microbiology and Infectious Diseases, The School of Medicine, University of Wales Swansea, Swansea, United Kingdom
- Stephen B Calderwood
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Affiliations Division of Infectious Diseases, Massachusetts General Hospital, Boston Massachusetts, United States of America , Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
- Frederick M Ausubel
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Affiliations Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, United States of America , Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America
- Costi D Sifri
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*To whom correspondence should be addressed. E-mail: csifri@virginia.edu
Affiliations Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, Virginia, United States of America , Division of Infectious Diseases, Massachusetts General Hospital, Boston Massachusetts, United States of America
Competing Interests
The authors have declared that no competing interests exist.
Author Contributions
B, FMA, and CDS conceived and designed experiments. HR and DM provided necessary reagents, constructed the complemented strain 9142-M10(pTXica), and performed polystyrene microtiter plate adherence assays. JMG carried out nematode quantification and pathogenesis experiments for some pulse-chase and sek-1 experiments. JB and CDS performed all other experiments. JB, FMA, SBC, and CDS analyzed the results and wrote the paper.