Seedless Aβ1–42 was incubated alone (black) or together with fibrillary Aβ1–42 seeds previously incubated with (green) or without (red) a fivefold molar excess of Mosd1. ThT (20 μM) was added to each sample in order to measure fibrillar content. The data were fitted with an asymmetric five parameter fit. The (A) amplitude of relative fluorescence (RFU) of fibrillated seedless Aβ1–42 served as 100% to which the other values were normalized. The (B) half-life (t1/2), displaying the point in time, when half of the maximum ThT signal (i.e. fibrillary content) was reached. Statistical significance was determined by one-way ANOVA. Error bars display SEM. ns: p > 0.05; *: p ≤ 0.05; **: p ≤ 0.01; ***: p ≤ 0.001.
Overview and detailed pictures of wild type Neuro-2a cells are shown. Neuro-2a cells were treated with 0, 10 and 100 μM of Mosd1, respectively. The left panel shows untreated wild type Neuro-2a cells. In the middle and right panel, incubation of wild type Neuro-2a cells with 10 μM Mosd1 and 100 μM Mosd1 are shown. Cell viability and morphology were analyzed with a LSM 710 laser scanning microscope. Scale bars equate 50 μm.
Overview and detailed pictures of Neuro-2a cells, stably transfected with human APP695, are shown. Cells were treated with 0, 10 and 100 μM of Mosd1, respectively. The left panel shows untreated hAPP695-transfected Neuro-2a cells. In the middle and right panel, incubation of hAPP695-transfected Neuro-2a cells with 10 μM Mosd1 and 100 μM Mosd1 are shown. Cell viability and morphology were analyzed with a LSM 710 laser scanning microscope. Scale bars equate 50 μm.
Citation: Rudolph S, Klein AN, Tusche M, Schlosser C, Elfgen A, Brener O, et al. (2016) Correction: Competitive Mirror Image Phage Display Derived Peptide Modulates Amyloid Beta Aggregation and Toxicity. PLoS ONE 11(7): e0159470. https://doi.org/10.1371/journal.pone.0159470
Published: July 12, 2016
Copyright: © 2016 Rudolph et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.