Repellents serve an important role in bite protection. Tick repellents largely rely on biomechanisms that induce responses with direct contact, but synthetic pyrethroids used as spatial repellents against insects have received recent attention for potential use in tick protection systems. An in vitro vertical climb assay was designed to assess spatial repellency against Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis adult, female ticks. Climbing behavior was assessed with and without the presence of two spatial repellents, transfluthrin and metofluthrin. Repellency parameters were defined to simulate the natural questing behavior of ambushing ticks, including measures of detachment, pseudo-questing duration, climbing deterrence, and activity. Significant effects were observed within each parameter. D. variabilis showed the greatest general susceptibility to each repellent, followed by A. americanum, and I. scapularis. The most important and integrative measure of repellency was climbing deterrence–a measure of the spatial repellent’s ability to disrupt a tick’s natural propensity to climb. Transfluthrin deterred 75% of D. variabilis, 67% of A. americanum, and 50% of I. scapularis. Metofluthrin was slightly more effective, deterring 81% of D. variabilis, 73% of A. americanum, and 72% of I. scapularis. The present study poses a novel paradigm for repellency and reports a preliminary assessment of spatial repellent effect on tick behavior. Further research will assess spatial repellency in a more natural setting, scale exposure conditions, and incorporate host cues.
Citation: Siegel EL, Olivera M, Roig EM, Perry M, Li AY, D’hers S, et al. (2022) Spatial repellents transfluthrin and metofluthrin affect the behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis in an in vitro vertical climb assay. PLoS ONE 17(11): e0269150. https://doi.org/10.1371/journal.pone.0269150
Editor: Catherine A. Brissette, University of North Dakota School of Medicine and Health Sciences, UNITED STATES
Received: May 13, 2022; Accepted: October 24, 2022; Published: November 8, 2022
Copyright: © 2022 Siegel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: This study was supported by the DoD Tick-borne Disease Program from the Congressional Directed Medical Research Program, grant # W81XWH1920028. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Ticks are the principal arthropod vectors of many prevalent zoonotic diseases in North America, including Lyme disease, anaplasmosis, and babesiosis [1, 2]. The incidence of tick-borne disease, though vastly under-reported, has risen dramatically in the past decade. Estimates in the United States illustrate this trend, with yearly incident case counts growing from 20,000 (2004) to 50,000 (present) [3, 4]. This rise is due to a combination of entomological factors that promote natural disease spread (including increases in tick geographical range/population density and shifts in host population dynamics) and human behaviors that constitute risk factors (such as pet ownership, outdoor activity, and the absence or misuse of personal protective measures) [5–7]. Addressing the burden of tick-borne disease therefore requires interdisciplinary approaches that target anthropogenic and entomological contributions to disease transmission .
Tick bite defense relies on personal protection measures . They include simple, cost-effective strategies that at-risk individuals can use to limit exposure or contact time with ticks. Tactics may involve performing tick checks, running clothing worn outdoors in a hot dryer, and repellent or acaricide use [10, 11]. The use of repellents is an efficacious and affordable option for protection . In many cases, repellents offer protection from a range of disease-causing, hematophagous arthropods, making them especially useful in regions of overlapping vector presence [13–15]. DEET (N, N-diethyl-3-methylbenzamide) is the most prominent commercial arthropod repellent and the gold standard against which novel formulations are compared . It deters mosquitoes from landing on a treated surface by interfering with odorant receptors on antennae that respond to host cues [17, 18]. In ticks, however, DEET has been described to work as a contact irritant–with varied efficacy across species [19, 20]. Another commonly used repellent is permethrin, a synthetic pyrethroid that kills and repels mosquitoes with direct droplet contact . It is also used as a clothing treatment for long-lasting tick protection through acaricidal action, with little signs of true repellent function .
“Repellency” warrants clarification, as the aforementioned examples show that products with varying biomechanisms (such as killing, attraction inhibition, and irritation) are often collectively referred to as “repellents” . Mosquitoes and other insects are fast-moving and spend mere seconds on a host . Repellency efforts that target mosquitoes are consequently focused on actions that prevent landing on a host [25, 26]. Ticks in comparison are relatively slow moving, do not fly, and may spend days attached to a host. The strict sense definition of repellency–wherein the arthropod makes an oriented movement away from the repellent source upon chemoreception–is therefore not always applicable with ticks . A more appropriate definition of repellency in ticks should encompass all modifiable behaviors that may prevent disease transmission throughout the tick-borne disease risk pathway. These include the actions that prevent moving onto a host, locating a favorable site of feeding, and inserting mouthparts for feeding/disease transmission [23, 28].
Repellents can be further classified by their mode of action as contact and spatial repellents depending on whether a target must contact a treated surface or react by non-tactile means to the volatilized state [29–31]. Active ingredients derived from synthetic pyrethroids are frequently used as spatial repellents against vectors that cover large distances, such as mosquitoes and biting flies [32, 33]. The efficacy of these spatial repellents is dependent on inherent arthropod sensitivity and the ability to maintain a consistent vapor phase concentration, which is often managed with controlled release devices (CRDs) that modulate dispersion [34–36]. Tick research has largely focused on contact repellency, leading to the development of guidelines for the quantitative evaluation of contact repellency, with clear standards guiding measurement and interpretation . Recent investigations, however, have explored the ability of spatial repellents to affect tick behavior [38–42]. Two synthetic pyrethroids, transfluthrin and metofluthrin, have demonstrated the ability to affect tick behavior through repellent and acaricidal properties in the vapor phase [40–42]. Information gained from these studies has led to the need for further evaluation of spatial repellent efficacy and the value these products may hold in personal and environmental protection systems. The present study sought to contribute to the understanding of spatial repellency in ticks by (1) defining an in vitro vertical climb assay with specific output parameters that quantify climbing behavior in ticks; (2) evaluating the ability of two CRD-delivered pyrethroids, transfluthrin and metofluthrin, to affect the climbing behavior of Dermacentor variabilis, Amblyomma americanum, and Ixodes scapularis based on these parameters; (3) making recommendations for future work for spatial repellency evaluation in ticks.
2. Materials and methods
2.1 Spatial repellents
Two spatial repellents were tested: metofluthrin (in-house, 97.15% technical grade: 91.96% Z-Isomer, 5.19% E-Isomer) and transfluthrin (98.68% technical grade, Bayer Corporation, Pittsburgh, PA, USA). Isopropanol (absolute, Sigma-Aldrich, St. Louis, MO, USA) was used as the solvent in preparation of 30% (w/v) formulations of each spatial repellent.
162 pathogen-free adult, female ticks: (60 D. variabilis, 48 A. americanum, and 54 I. scapularis) were used and originated from the tick-rearing facility at the Oklahoma State University, Department of Entomology and Plant Pathology, National Tick Research and Educational Resource.
2.2.2 Storage and handling.
Ticks were stored in 48 mm (h) x 20 mm (w) Fisherbrand polystyrene containers with plastic snap caps. A 5 mm diameter orifice was punched into each top. This hole was covered with woven, nylon mesh (Voile White Decorator Fabric) to prevent ticks from escaping. Ticks were stored four to a vial, and a chilled coma was maintained at 4°C until the ticks were used. The containers were removed from refrigeration weekly and opened for 10 minutes. The cloth, vials, and containers were checked thoroughly at this time for evidence of fungal growth. If evidence of growth was noted on the vials, the ticks were moved to clean vials. Ticks were handled with autoclaved forceps and paintbrushes. Two hours preceding the repellency trial, ticks were removed from refrigeration and equilibrated in an incubator (Percival 1-30VK environmental chamber) at 23°C at 90% relative humidity.
2.3 Experimental setup
2.3.1 The controlled release device.
The CRD consisted of a 4.5 cm (l) x 1.5 cm (w) x 2.0 cm (z) 3D printed frame with three porous reservoirs (Fig 1). The three reservoirs (1 cm diameter, 1.5 cm depth) each contained one piece of cellulose sponge (0.5 cm (l) x 0.5 cm (w) x 0.1 cm (z)). The repellent was released through the 1 mm x 1mm square pore on the top face of the device frame.
2.3.2 Experiment chamber.
The tick behavior test system consisted of (1) a CRD, specifically designed for the sustained spatial release of test repellent formulations (Fig 1), (2) a tick behavioral test chamber (Fig 2), and (3) a computer-based movement tracking system (Ethovision XT, Version 15.0, Noldus Information Technology, Leesburg, VA, USA). The tick behavior test chamber was assembled from six clear, acrylic sheets. Four 60 cm (l) x 30 cm (w) x 0.5 cm (z) sheets formed the bottom, top, front, and back faces, and two 30 cm (l) x 30 cm (w) x 0.5 cm (z) sheets formed the sides. Sheets were connected by 90-degree angled plastic hinges, creating a static air chamber. The hinge connectors created a 0.5 cm space around the perimeter of the box, allowing for ventilation.
The controlled release device was placed in the upper left-hand corner of the experiment chamber. Three vertical climbing sticks were arrayed along the top face. A camera recorded tick climbing from the perspective shown for quantitative behavior analysis based on vertical movement.
Three sticks (Simoutal brand polyester-cotton fiber diffuser sticks), 30.5 cm (l) x 0.3 cm (diameter), were used for each climbing experiment. The sticks were adhered to the inside of the top of the chamber by a 1 cm x 1 cm square piece of Crayola air-dry clay, cut with a sterile #10 scalpel. There was a 1.5 cm space between the bottom of the stick and the bottom of the chamber. For a tick to reach the bottom surface of the chamber, it would therefore have to detach and fall from its stick. The sticks were arrayed along the center width of the lid, one centered and the other two 2.5 cm from either side. After each trial, the climbing sticks and clay were discarded, and the walls of the chamber were cleaned with isopropanol (70%).
2.3.3 Repellency vertical climb bioassay.
The 30% formulation of transfluthrin or metofluthrin (50 μl) was loaded into the middle reservoir of the CRD. The CRD was then adhered to the upper end of the chamber. The repellent was allowed 20 minutes to release into the box from the CRD prior to the introduction of the ticks for the trial (hereafter “induction time”). Trials included 3 female ticks of the same species: one tick per climbing stick.
Ticks were evaluated for inclusion by briefly placing them at the base of an untreated stick. If the tick climbed the stick, it was included in the trial. Ticks were excluded in the rare event that ticks were unable to hold on to the stick, detached, or were unwilling to climb. Climbing trials were conducted by removing the chamber lid with the attached climbing sticks, inverting it, and placing the ticks on the climbing sticks. The entire lid with three climbing sticks with ticks at top was then inverted and placed on the walls of the chamber so that the ticks started the trial at the bottom. Tick behavior was observed for 10 minutes. Treatments were compared to controls performed in the same fashion in the absence of repellents.
2.4 Video tracking of tick movements
Tick mobility was tracked with a computer vision system . This included a camera (Basler acA1300-60gm) connected to a desktop (Dell Precision 3630) running EthoVision XT. Each experiment was generated with a pre-defined template, maintaining a constant capture rate (3.5 frames per sec.), camera field of view, and detection criteria for tracking. Height was recorded frame by frame during the trial. Minor inaccuracies in tick movement recorded by the tracking system were corrected prior to analysis.
2.5 Activity parameters and measures of spatial repellency
Four parameters were defined to measure climbing behavior. These included an analysis of movement and velocity, climbing deterrence, pseudo-questing, and detachment. Each measure was designed to simulate a component of vertical climbing associated with the host-seeking process.
2.5.1 Activity analysis.
All ticks received a binary score based on if they moved at all during the trial. Ticks that remained stationary at the bottom of their stick during the entire trial were scored “immobile” and ticks that moved at all during the trial were scored “mobile.”
Ticks scored “mobile” were considered in a further analysis to see if exposure to the spatial repellents resulted in changes to movement speed. The mean velocity was calculated for each tick from digested height data over the duration of the experiment. Only time points where the tick was moving were included in the analysis. Transfluthrin and metofluthrin groups were compared to controls using the Wilcoxon Rank-Sum Test (WRST) to obtain P values . Pearson’s r was used as a measure of effect size to quantify the magnitude of significant associations .
2.5.2 Climbing deterrence.
Ticks were individually scored “not deterred” or “deterred” according to their ability to successfully climb their stick. A tick that was scored “not deterred” was successful in climbing. This required the tick to (1) reach the top of the stick at some point in the trial, and (2) not detach from the stick at any point in the trial, and (3) have a meaningful presence at the top, indicated by the tick staying at the top of the stick or climbing onto the top surface of the box. Ticks were scored “deterred” if they (1) did not reach the top of the stick by the end of the trial, or (2) fell off the stick at any point in the trial, or (3) once reaching the top of the stick, moved down. The total “deterred” and “not deterred” in each group were added and presented as proportions. P values were calculated from the difference in proportions between controls and repellent groups with a two-tailed Fisher’s exact test. Supplementary effect size calculations were done using Cramer’s phi (φ) .
Ticks were scored “detached” if they fell from their stick at any point in the trial. Ticks were scored “not detached” if they did not fall from their stick. Fisher’s exact tests and φ values were calculated to evaluate the degree of difference in detachment between controls and repellent groups.
2.5.4 Pseudo-questing duration.
A pseudo-questing measure was considered to simulate the questing behavior of ambushing ticks. The cumulative duration (in sec.) that each tick was found above the 27 cm mark on their stick was calculated and considered analogous to the time that ticks would spend atop tall grass or other surfaces in the host-seeking process. Time that ticks spent on the lid was included in this measure. The WRST and Pearson’s r were used to evaluate the difference between controls and repellent groups.
2.6 Data analysis
Analyses were performed in SPSS for Windows, Advanced Statistics, Version 28.0 . Results of WRSTs were considered significant in cases where W < Wcrit at a significance threshold α = 0.05, following SAMPL guidelines [44, 48]. Cohen’s classification of effect size (for r and φ) at 1 degree of freedom (0.1–0.3 small, 0.3–0.5 medium, and > 0.5 large) provides a standardized interpretation of effect [49, 50]. However, the application to biological investigations varies from system to system . Considering the protective effect expected of a trusted repellent, effect sizes greater than or equal to 0.80 were considered large and of substantial biological significance. Medium effect sizes were considered for values between 0.60 and 0.80. Small effect sizes were considered for values less than 0.60. Output graphs were produced in GraphPad Prism, Version 9.4.1 for macOS .
2.7 GCMS and in silico simulation
Transfluthrin and metofluthrin release rates from the CRD were characterized using analytical formulations, assessing volatilization for 30 minutes, equal to the experiment duration (20-minute induction time and 10-minute trial). For this measurement, the CRD was placed in a sealed plastic bottle and a 50 mL air sample was extracted via syringe chemistry tools based on Gas Chromatography-Mass Spectroscopy Analysis (GCMS). Extracted air samples were dissolved in 5 mL of isopropanol (absolute) and injected directly into the GCMS for the measurement of chemical concentrations relative to a known standard. Numerical simulations based on computational fluid dynamics (CFD) were also performed. These addressed the transport of evaporated transfluthrin and metofluthrin into still air inside the test chamber. Fluid natural convection, chemical diffusion, and gravity momentum were considered. Detailed information and mathematics on the CFD simulations can be found in supplemental information (S1 and S2 Figs).
3.1 GCMS and CFD simulation
Release rates obtained from the GCMS experiments were 3.1 mg/hr for transfluthrin and 4.4 mg/hr for metofluthrin. Concentration plots from the CFD simulation for both formulations were plotted on a logarithmic scale for a snapshot in time, 25 minutes post-release (Fig 3). Exposure concentrations along the length of each stick were extrapolated from these measurements (Fig 4). Though a slight horizontal concentration gradient was observed, the magnitude of the difference in exposure between the three sticks was not noteworthy. Higher concentrations were shown to move precipitously to the bottom of the chamber due to a dispersion flow dominated by gravity. Further, the vapor density of the formulation was greater than that of the still air in the chamber, and this helped push the flow downward immediately from the CRD. Over time, however, the concentration diffused from the bottom of the box to the top, resulting in the formation of a concentration gradient. This gradient persisted over time due to the influence of gravity and the low diffusion rate.
The concentration gradients produced from the CFD simulation were plotted for (A) transfluthrin, and (B) metofluthrin, 25 minutes post-release. Accounting for the 20-minute induction time prior to tick introduction, the concentrations represented tick exposure halfway (5 minutes) through the trial. A vertical concentration gradient was seen, with higher concentrations (warmer colors) present on the bottom of the chamber and lower concentrations (cooler colors) at the top. Two perspectives were illustrated: looking at the chamber from the perspective of the camera (right) and the side with the CRD (left). The CRD was positioned in the top left corner from the perspective of the right box and the upper middle of the side perspective.
Stick position was correlated with the gradients produced in the CFD simulations to show the exposure concentrations on the y-axis (in ppb) with matching position (in cm) along the length of each stick. This was taken 25 minutes post-release (halfway through the 10-minute trials following a 20-minute induction time) for (A) transfluthrin; and (B) metofluthrin.
3.2 Repellency parameters
3.2.1 Activity analysis.
All ticks survived the experiments and a period of post-study observation. There was however a noticeable tendency of ticks of each species exposed to transfluthrin to retain a drunken-like state once being removed from the chamber. Ticks presented this behavior by randomly flipping onto their dorsum and showed an inability to walk and climb normally. This effect was not seen in metofluthrin-exposed or control ticks.
Consistent with pre-trial inclusion criteria ensuring that ticks climbed when placed at the base of an untreated stick, all ticks in the control groups for each species were mobile for at least a portion of their trial, readily climbing the stick when placed in the chamber. Each treatment group in contrast showed varying proportions of ticks that remained immobile at the stick bottom for the entirety of the trial, despite showing a willingness to climb just moments prior. This was more prevalent in metofluthrin- exposed ticks than transfluthrin-exposed in each species. 4/21 (19%) of D. variabilis exposed to metofluthrin and 3/24 (12.5%) exposed to transfluthrin remained immobile. 4/15 (27%) of A. americanum exposed to metofluthrin and 1/18 (6%) exposed to transfluthrin remained immobile. 2/18 (11%) of I. scapularis exposed to metofluthrin and 1/18 (6%) exposed to transfluthrin remained immobile. These proportions were not large enough to show significance, however they dictated the sample that was used in the analysis of velocity while moving.
The velocity analysis included the remaining ticks that were scored mobile. Results showed that exposures to both transfluthrin and metofluthrin were associated with significant reductions in tick velocity while moving for D. variabilis (Mdncontrol = 0.365 cm/s; Mdnmeto = 0.111 cm/s: P < 0.001, r = 0.79; Mdntrans = 0.110 cm/s: P < 0.001, r = 0.82) and A. americanum (Mdncontrol = 1.041 cm/s; Mdnmeto = 0.377: P < 0.001, r = 0.66; Mdntrans = 0.445: P < 0.001, r = 0.71) (Fig 5). The large effect observed with D. variabilis also greatly reduced the spread of velocity from the otherwise dispersed control (IQR = 0.185 cm/s), condensing the group around a lower median (IQRmeto = 0.095 cm/s, IQRtrans = 0.098). The opposite effect was observed with A. americanum in the metofluthrin group, where the sample, though having a lower median, had an IQR almost twice that of controls (IQRmeto = 0.658 cm/s, IQRcontrol = 0.347 cm/s). Transfluthrin exposure in comparison did not affect the spread of A. americanum velocity (IQRtrans = 0.356 cm/s). Weak, non-significant reductions were observed with both repellents for I. scapularis.
Boxplots were made to describe the distribution of tick velocity (in cm/s) while moving along the stick. Points greater than 1.5 x IQR in each group were considered outliers but included in the WRST analysis. Ticks that did not move were excluded. Groups included: N = 147 (D. variabilis: 15 control, 17 metofluthrin, 21 transfluthrin; A. americanum: 15 control, 11 metofluthrin, 17 transfluthrin; I. scapularis: 18 control, 16 metofluthrin, 17 transfluthrin). Significant reductions were shown with transfluthrin and metofluthrin in D. variabilis and A. americanum. Outliers: • values greater than 1.5x IQR. Significance indicator: NS = Not significant, * P < 0.05, ** P < 0.01, *** P < 0.001.
3.2.2 Tick detachment.
Exposure to metofluthrin and transfluthrin did not generally affect the tendency of ticks to detach from their sticks (Fig 6). The sole significant difference was observed with metofluthrin against I. scapularis, where 33% (6/18) of ticks detached compared to 0% (0/18) of controls, P = 0.019. The size of this effect, though non-negligible, was not large enough to translate into a biologically notable measure when considering repellency standards (φ = 0.44).
The proportion of ticks that detached from their stick was assessed and shown as a percentage for each group. All ticks were included in the analysis. Only exposure to metofluthrin was associated with a small, significant increase in detachment in I. scapularis. Significance indicator: NS = Not significant, * P < 0.05, ** P < 0.01, *** P < 0.001.
3.2.3 Climbing deterrence.
A significant climbing deterrence was observed in both treatment groups for all three species (Fig 7). 13% (2/15) of control D. variabilis were not deterred in comparison to 81% (17/21) of metofluthrin-exposed (P < 0.001, φ = 0.67) and 75% of transfluthrin-exposed (P < 0.001, φ = 0.60). A similar effect was observed in A. americanum trials. 13% (2/15) of controls were not deterred compared to 73% (11/15) of metofluthrin-exposed (P < 0.001, φ = 0.66), and 67% (12/18) of transfluthrin-exposed (P = 0.002, φ = 0.54). Metofluthrin performance in I. scapularis was comparable to that of A. americanum and showed 72% (13/18) deterrence when compared to 1/18 (6%) of controls (P < 0.001, φ = 0.68). Transfluthrin exposure in I. scapularis was associated with the weakest deterrence, though significant, with 50% (9/18) ticks having been deterred (P = 0.003, φ = 0.50).
The proportion of ticks deterred was described as a percentage. All ticks were included in the analysis. Significant deterrence was observed with transfluthrin and metofluthrin in all species. Significance indicator: NS = Not significant, * P < 0.05, ** P < 0.01, *** P < 0.001.
3.2.4 Pseudo-questing (P-Q) duration.
Control trials showed that the ticks of each species spent most of the trial at the top of the stick or climbed from the top of the stick to the top face of the chamber to remain (Fig 8). Metofluthrin and transfluthrin showed very similar reductions in D. variabilis pseudo-questing (P-Q) duration (Mdnmeto = 0 sec.; Mdntrans = 0 sec.) when compared to controls (Mdncontrol = 495 sec.) (P < 0.001, r = 0.76). The spread of metofluthrin-exposed ticks was however more concentrated around the median (IQR = 71 sec.), in comparison to the similarly spread control and transfluthrin groups (IQRcontrol = 171 sec., IQRtrans = 189 sec.).
Time that ticks spent above the 27 cm mark on the stick was considered time pseudo-questing (P-Q). Boxplots showed pairwise comparisons of repellent trials and controls, with cumulative P-Q duration (in sec.) of ticks in each group shown on the y axis. Significant reductions in P-Q duration were observed in both repellent groups in D. variabilis and A. americanum. I. scapularis showed a small, significant reduction in the presence of metofluthrin, but transfluthrin was not associated with a significant change. Outliers: • values greater than 1.5x IQR. Significance indicator: NS = Not significant, * P < 0.05, ** P < 0.01, *** P < 0.001.
Metofluthrin-exposed A. americanum showed a reduction in P-Q duration comparable to D. variabilis (Mdnmeto = 0 sec., Mdncontrol = 505 sec., P < 0.001, r = 0.76). A slightly weaker, but still significant reduction was seen in the transfluthrin group (Mdntrans = 98 sec., P < 0.001, r = 0.63). Contrasting the narrowing effect on dispersion seen against D. variabilis, the spread of P-Q duration in the A. americanum metofluthrin group (IQR = 147 sec.) was like that of the controls (IQR = 174 sec). Transfluthrin exposure in contrast increased P-Q duration spread (IQR = 283 sec.).
Reductions in P-Q duration in treatment groups were much smaller in I. scapularis when compared to D. variabilis and A. americanum. A weak, significant reduction was observed in metofluthrin trials (Mdncontrol = 401 sec., Mdnmeto = 171 sec., P = 0.016, r = 0.40) while that observed with transfluthrin was not significant. Like the other species, I. scapularis controls were moderately spread (IQR = 104 sec.). Metofluthrin and transfluthrin exposure increased the spread of the groups (IQRmeto = 407 sec., IQRtrans = 387 sec.). Some ticks were relatively unaffected, having a P-Q duration comparable to that of controls, while others were observed to be more deterred.
Significant effects were observed within each repellency parameter. Observations varied in strength by repellent group and by species. D. variabilis was generally more affected in each parameter and I. scapularis the least, except for in the detachment analysis, where the only significant difference with exposure occurred with metofluthrin in I. scapularis. Metofluthrin overall was associated with stronger performances within each indicator than transfluthrin. Both spatial repellents, however, showed the ability to deter climbing and pseudo-questing as well as distort tick movement as seen with reductions in velocity. It is important to consider different perspectives when measuring tick repellents, as they may work through different biomechanisms and therefore show their effects in different ways. Some measures will, however, be more descriptive than others. The most important parameter defined in the present study was climbing deterrence, which integrated several behaviors, however each parameter pertained to the host-seeking process in a different way.
The CFD simulation indicated the formation of a discernible vertical concentration gradient in the test chamber. This understanding allowed tick behavior to be connected to exposure. Deterrence of natural behavior to climb up was shown in several ways to be immediately affected upon tick placement into the chamber, where the concentration was the highest. Some remained immobile here for the entire trial. Further, significant reductions in velocity were seen as soon as ticks began climbing. The lack of tick movement opposing the concentration gradient indicated that the repellents did not act as a movement barrier but instead immediately disrupted favorable movement patterns aimed at the top of the box through an intoxication-like biomechanism. From this, assay changes would need to be made to assess the use of these repellents in a protective barrier role. Ticks would need to be placed in the chamber and allowed to climb into the repellent cloud. This can be done by reducing the induction time prior to tick introduction, increasing the height of the climbing sticks and chamber, and modifying the position of the CRD. Dose-stratified studies would also be required for further interpretation of the concentration gradient observed and to identify minimum and optimal concentrations for each spatial repellent, as the effective concentrations may differ from those used in the present study.
Host-seeking for ambushing ticks occurs through a process called questing, where they climb to the top of tall grass or other objects, hold their front legs out, and await a passing host on which to grab . Once on host, they navigate upwards in search of a feeding site. Vertical repellency assays simulate this behavior, exploiting the tendency of ticks to climb [19, 38, 54–56]. In nature, a tick would be required to climb vertically without detaching from their climbing surface–whether this be on grass or host. The proportion of ticks deterred from climbing could therefore apply to both settings. Detachment is another described indicator of inhibition in the host-seeking and feeding behavior of ticks . If a tick detaches, it is not feeding or transmitting disease, whether this be from the top of grass it is questing on, while walking on the host, or early in the feeding process with its mouthparts inserted. In the context of spatial repellency evaluation, this could be evidence of the spatial repellent affecting the tick to the point where it could not function normally.
Questing behavior is naturally influenced by abiotic factors, including light, humidity, and temperature . Ticks also have a limited supply of energy that once depleted cannot be restored. Therefore, ticks balance energy expenditure with the risks of questing by concentrating activity around conditions favorable to host-seeking and avoiding mortality . Assuming ticks can differentiate conditions appropriate for questing from those that are not, it may be hypothesized that a reduction in questing time or increased immobility at lower heights may be evidence of questing deterrence.
Velocity, though unable to serve as a direct measure of repellency, can provide a measure of movement changes in the presence of spatial repellents. Observations in the present study showed this with reductions in velocity immediately when exposed. We can also observe differences innate to tick species, where A. americanum are naturally more fast-moving than D. variabilis and I. scapularis. Those that did not move at all in the trial, though not representing a statistically significant subset, may also represent ticks that were so affected by the high concentration of exposure that they were unable to proceed up the stick.
There are no spatial repellent products currently employed to protect from tick bites. Synthetic pyrethroids (metofluthrin, transfluthrin, allethrin) are used in passive CRDs (OFF! Clip-On Device, Personal Insect Repellent Kit [PIRK], ThermaCELL) as spatial repellents to protect against mosquitoes and other insects [40–42]. Recently, investigations into tick response to these devices have demonstrated varying spatial repellent effects and acaricidal activity in several species of ticks. I. holocyclus adult, female ticks have exhibited susceptibility to the OFF! Clip-On Device (31.2% metofluthrin) and ThermaCELL (21.9% allethrin), with exposures associated with spatial repellency and post-exposure mortality in the laboratory setting . In a more natural environment, however, these effects were diminished. . This shows the importance of studies performed in the natural setting, where external factors may impact spatial repellency. Other studies have shown similar effects with ThermaCELL, OFF! Clip-On Device, and PIRK (15.6% transfluthrin) against different life stages of I. scapularis, A. americanum, and D. variabilis in laboratory conditions without host-cues [41, 42]. Metofluthrin and transfluthrin in these experiments were associated with varying levels of mortality and spatial repellency between species. Neither repellent was associated with any mortality, during or after exposure. Further, the species-level susceptibility to the repellents appeared contrary to what has previously been demonstrated, with D. variabilis appearing the most affected and I. scapularis the least in our study. Consistent results however were shown with deterrence and observed behavior otherwise. Life stages, experimental conditions, tick sourcing, repellent formulation/release rate/induction time, and experiment duration among other factors must be taken into consideration when comparing the results of spatial repellent studies.
Chemoreception in ticks occurs through gustatory and olfactory mechanisms that are not well-understood . A repellent biomechanism may work through one or both processes but tick repellent studies do not (or are not often able to) determine which is (are) at play . The Haller’s Organ is a complex sensory organ found on the tarsal segment of the front legs and is the basis of tick olfaction [53, 60–62]. It is comprised of two major structures: an anterior pit that detects humidity and a capsule fitted with host-cue sensing, physiologically diverse olfactosensilla. The response to repellents, including DEET, has been shown to diminish with the occlusion or surgical removal of the Haller’s Organ, suggesting that the Haller’s Organ plays a role in the repellent response [60, 63]. Conversely, ticks have shown equal response to other repellents including phenethyl alcohol (PEA) with the Haller’s Organ removed–alluding to other structures playing important roles in repellent chemoreception . Considering PEA, removal of the pedipalps impairs repellent response. Further research is needed to characterize the molecular mode of action in tick olfaction and gustation to complement the analysis of novel tick-targeting repellents.
Although this represents a knowledge gap at the molecular level, it has been extensively demonstrated that tick behavior and response to biochemical stimuli can vary by species and life stage. Ticks of two species may show differences in responses or susceptibility to the same stimulus, under the same assessment conditions [64, 65]. This may be attributed to the size of the species or morphological differences in gustation and olfaction structures between species. Another important consideration pertains to the observable behavior of some species. Aggressive, fast-moving species, such as A. americanum, are often thought to produce a general underestimation of true susceptibility to repellents due to their higher speed and agility . For instance, they may “blow past” a thin treatment line or barrier before they can even sense it.
Laboratory-reared ticks may also respond differently to repellents than those to which humans would be exposed. These differences may occur due to variation in age (ticks in the environment will have a more heterogeneous distribution of age) and genetic differences, highlighting a need to consider natural variation in future investigations . The laboratory-reared ticks used in the present study were pathogen-free. It has been demonstrated that pathogens can modify the phenotype of their arthropod vector hosts in ways that benefit the pathogen. Some of these mechanisms increase vector competence by modifying vector behavior .
Considering insects, infections of Plasmodium spp. in Anopheles mosquitoes and Leishmania spp. in Lutzomyia sandflies are associated with molecular changes in olfactory mechanisms that modify host-seeking ability . Similar mechanisms have not yet been described in ticks, but activity differences have been shown between pathogen-infected ticks and pathogen-free ticks that are relevant in the context of repellency [70–72]. Borrelia burgdorferi and Anaplasma phagocytophilum, the disease-causing pathogens for Lyme disease and anaplasmosis, have been shown to increase questing duration and walking activity in I. scapularis and I. ricinus adults and nymphs [70, 71]. Further, tick-borne encephalitis virus has been shown to increase DEET tolerance of I. ricinus . Repellency testing should proceed to account for these factors to provide appropriate estimates for the level of protection humans may receive with exposure to pathogenic ticks.
The external validity of in vitro evaluations of repellency is limited in the absence of host cues and natural conditions. Preliminary assessments of repellent function are best performed in the laboratory setting for safety and economic purposes; however, external factors may impact protective effects as it is currently unknown how wind or human movement around the CRD may affect the protective barrier. Other factors, such as temperature and humidity, may affect the extent to which the target vector responds and disrupt the gradient barrier with changes in vapor pressure. Because the stability of the volatilized concentration serves as the basis of a protective barrier with spatial repellents, tests in natural conditions are an essential step in product development. The present study represents the earliest stages in repellent testing and a point from which to scale. Future work should assess effects in larger, more representative populations and in more natural environments. In practical use, it would be hypothesized that these factors would not affect the spatial concentration around the device enough to reduce the repellent effect, allowing the CRD to serve as a wearable barrier to tick bites. This, however, has yet to be effectively demonstrated.
Management practices and personal protection lie at the forefront of tick bite prevention, as a “magic bullet” solution for tick-borne disease does not yet exist [8, 53, 73]. At the individual level, repellent use is one of the most important tactics for protection. DEET and permethrin are the most widely used repellents in tick protection. Due to limitations with safety concerns, imperfect human agreeability, and inconsistency of protective duration and efficacy across tick species, new repellents are still needed . The ideal repellent requires an active ingredient formulated to provide long-lasting, efficacious protection against a range of disease-transmitting vectors in a safe, pleasant formula for human use [53, 74]. Spatial repellency research to date has set a path for future work which may provide humans with novel, efficacious spatial repellents to target ticks. The present study has identified several parameters which may be used to assess spatial repellency, considering the natural questing behavior of ambushing ticks, and demonstrated significant effects on tick behavior with transfluthrin and metofluthrin based on these parameters.
S1 Fig. CFD simulation mesh: Front, right, bottom, isometric views.
S2 Fig. Detailed view of the mesh used around the device, isopropanol, and repellent source in CFD simulation.
S1 Table. Properties and input flow rates for transfluthrin, metofluthrin, and isopropanol.
S1 Appendix. CFD and GCMS.
S1 Data. Raw data from climbing trials.
The authors would like to thank Mr. Kevin Smith as representative of Bayer Environmental Sciences for providing the technical transfluthrin to conduct the research. His enthusiasm and corporate contribution are greatly appreciated.
This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation by the authors, USDA, or DoD for its use. The USDA is an equal opportunity provider and employer.
- 1. Rochlin I, Toledo A. Emerging tick-borne pathogens of public health importance, a mini-review. J Med Microbiol. 2020;69(6): 781–791. pmid:32478654
- 2. Madison-Antenucci S, Kramer LD, Gebhardt LL, Kauffman E. Emerging tick-borne diseases. Clin Microbiol Rev. 2020;33(2). pmid:31896541
- 3. Rosenberg R, Lindsey NP, Fischer M, Gregory CJ, Hinckley AF, Mead PS, et al. MMWR Mobr Mortal Wkly Rep. 2018;67(17): 496–501.
- 4. Beard CB, Eisen L, Eisen RJ. The rise of ticks and tickborne diseases in the United States–introduction. J Med Entomol. 2021;58(4): 1487–89. pmid:33939806
- 5. Paules CI, Marston HD, Bloom ME, Fauci, AS. Tickborne diseases–confronting a growing threat. N Engl J Med. 2018;379: 701–703. pmid:30044925
- 6. Connally NP, Ginsberg HS, Mather TN. Assessing peridomestic entomological factors as predictors for Lyme disease. J Vector Ecol. 2006 Dec;31(2):364–70. pmid:17249354
- 7. Fischhoff IR, Keesing F, Ostfeld RS. Risk factors for bites and diseases associated with black-legged ticks: a meta-analysis. Am J Epidemiol. 2019;188(9): 1742–1750. pmid:31145773
- 8. Richardson M, Khouja C, Sutcliffe. Interventions to prevent Lyme disease in humans: a systematic review. Prev Med Rep. 2019;13: 16–22. pmid:30456054
- 9. Eisen R. Personal protection measures to prevent tick bites in the United States: Knowledge gaps, challenges, and opportunities. Tick Tick Borne Dis. 2022;13(4): 101944.
- 10. Vasquez M, Muehlenbein C, Cartter M, Hayes EB, Ertel S, Shapiro ED. Effectiveness of personal protective measures to prevent Lyme disease. Emerg Infect Dis. 2008;14(2): 210–216. pmid:18258112
- 11. Evidence for personal protective measures to reduce human contact with blacklegged ticks and for environmentally based control methods to suppress host-seeking blacklegged ticks and reduce infection with Lyme disease spirochetes in tick vectors and rodent reservoirs. J Med Entomol. 2016;53(5): 1063–1092. pmid:27439616
- 12. Pages F, Dautel H, Duvallet G, Kahl O, de Gentile L, Boulanger N. Tick repellents for human use: prevention of tick bites and tick-borne diseases. Vector Borne Zoonotic Dis. 2014;14(2): 85–93. pmid:24410143
- 13. Diaz JH. Chemical and plant-based insect repellents: efficacy, safety, and toxicity. Wilderness Environ Med. 2016;27(1): 153–163. pmid:26827259
- 14. Katz TM, Miller JH, Hebert AA. Insect repellents: historical perspectives and new developments. J Am Acad Dermatol. 2008;58(5): 865–871. pmid:18272250
- 15. Carroll SP. Prolonged efficacy of IR3535 repellents against mosquitoes and blacklegged ticks in North America. J Med Entomol. 2008;45(4): 706–714. pmid:18714871
- 16. Leal WS. The enigmatic reception of DEET–the gold standard of insect repellents. Curr Opin Insect Sci. 2014;6: 93–98. pmid:25530943
- 17. Xu P, Choo Y-M. De La Rosa A, Leal WS. Mosquito odorant receptor for DEET and methyl jasmonate. Proc Natl Acad Sci. 2014;18(46): 16592–7. pmid:25349401
- 18. Costantini C, Birkett MA, Gibson G, Ziesmann J, Sagnon NF, Mohammed HA, et al. Electroantennogram and behavioural responses of the malaria vector Anopheles gambiae to human-specific sweat components. Med Vet Entomol. 2001;15(3): 259–66. pmid:11583442
- 19. Carroll JF, Solberg VB, Klun JA, Kramer M, Debboun M. Comparative activity of DEET and AI-37220 repellents against the ticks Ixodes scapularis and Amblyomma americanum (Acari: Ixodidae) in laboratory bioassays. J Med Entomol. 2004;41(2): 249–254. pmid:15061285
- 20. Schreck CE, Fish D, McGovern TP. Activity of repellents applied to skin for protection against Amblyomma americanum and Ixodes scapularis ticks (Acari: Ixodidae). J am Mosq Control Assoc. 1995;11(1): 136–140. pmid:7616181.
- 21. Bowman NM, Akialis K, Cave G, Barrera R, Apperson CS, Meshnick SR. Pyrethroid insecticides maintain repellent effect on knock-down resistant populations of Aedes aegypti mosquitoes. PLoS ONE. 2018;13(5). pmid:29763445
- 22. Lane RS. Treatment of clothing with a permethrin spray for personal protection against the western black-legged tick, Ixodes pacificus (Acari: Ixodidae). Exp Appl Acarol. 1989;6(4):343–52. pmid:2743839
- 23. Halos L, Baneth G, Beugnet F, Bowman AS, Chomel B, Farkas R. Defining the concept of ‘tick repellency’ in veterinary medicine. Parasitology, 2012;139(4):419–23. pmid:22216951
- 24. Tirados I, Costantini C, Gibson G, Torr SJ. Blood-feeding behavior of the malarial mosquito Anopheles arabiensis: implications for vector control. Med Vet Entomol. 2006;20(4): 425–437. pmid:17199754
- 25. Stevenson JC, Simubali L, Mudenda T, Cardol E, Bernier UR, Abad A, et al. Controlled release spatial repellent devices (CRDs) as novel tools against malaria transmission: a semi-field study in Macha, Zambia. Malar J. 2018;17:437. pmid:30477502
- 26. Ray A. Reception of odors and repellents in mosquitoes. Curr Opin Neurobiol. 2015;34: 148–164. pmid:26202080
- 27. Dethier VG, Browne LB, Smith CN. The designation of chemicals in terms of the responses they elicit from insects. J Econ Entomol. 1960;53(1): 134–136.
- 28. Bissinger BW, Roe RM. Tick repellents: past, present, and future. Pestic Biochem Phys. 2010;96(2): 63–79.
- 29. Norris EJ, Coats JR. Current and future repellent technologies: the potential of spatial repellents and their place in mosquito-borne disease control. Int J Environ Res Public Health. 2017;14(2): 124. pmid:28146066
- 30. Achee NL, Bangs MJ, Farlow R, Killeen GF, Lindsay S, Logan JG, et al. Spatial repellents: from discovery and development to evidence-based validation. Malar J. 2012;11. pmid:22583679
- 31. Bernier UR, Furman KD, Kline DL, Allan SA, Barnard DR. Comparison of contact and spatial repellency of catnip oil and N,N-Diethyl-3-methylbenzamide (Deet) against mosquitos. J Med Entomol. 2005;42(3): 306–311.
- 32. Andreazza F, Valbon WR, Wang Q, Liu F, Xu P, Bandason E, et al. Sodium channel activation underlies transfluthrin repellency in Aedes aegypti. PLoS Negl Trop Dis. 2021;15(7).
- 33. Verhulst NO, Cavegn JC, Mathis A. Spatial repellency and vapour toxicity of transfluthrin against the biting midges Culicoides nubeculosus and C. sonorensis (Ceratopogonidae). Current Research in Insect Science. 2021;1. pmid:36003605
- 34. Bernier UR, Gurman P, Clark GG, Elman N. Functional micro-dispensers based on micro-electro-mechanical-systems (MEMS) integrated with fabrics as functional materials to protect humans from mosquito feeding. J Control Release. 220A: 1–4. pmid:26415856
- 35. Bernier UR, Clark GG, Gurman P, Elman N. The use of microdispensers with spatial repellents for personal protection against mosquito biting. J Med Entomol. 2016;53(2): 470–472. pmid:26637385
- 36. Bernier UR, Kline D, Vazquez-Abad A, Perry M, Cohnstaedt LW, Gurman P, et al. A combined experimental-computational approach for spatial protection efficacy assessment of controlled release devices against mosquitoes (Anopheles). PLoS Negl Trop Dis. 2019. 13(3): e0007188. pmid:30856177
- 37. U.S. Environmental Protection Agency (EPA). (2010, July 7). Product Performance Test Guidelines: OPPTS 810.3700: Insect Repellents to be Applied to Human Skin [EPA 712-C-10-001]. Available: https://www.regulations.gov/document/EPA-HQ-OPPT-2009-0150-0011.
- 38. Eisen L, Rose D, Prose R, Breuner NE, Dolan MC, Thompson K, et al. Bioassays to evaluate non-contact spatial repellency, contact irritancy, and acute toxicity of permethrin-treated clothing against nymphal Ixodes scapularis ticks. Ticks Tick Borne Dis. 2017;8(6): 837–849. pmid:28754599
- 39. Bissinger BW, Apperson CS, Watson DW, Arellano C, Sonenshine DE, Roe RM. Novel field assays and the comparative repellency of BioUD, DEET, and permethrin against Amblyomma americanum. Med Vet Entomol. 2011;25: 217–226. pmid:21118283
- 40. Sukkanon C, Chareonviriyaphap C, Doggett SL. Topical and spatial repellent bioassays against the Australian paralysis tick, Ixodes holocyclus (Acari: Ixodidae). Austral Entomol. 2019;58: 866–874.
- 41. Bibbs CS, Xue R-D. ThermaCELL and OFF! Clip-On devices tested for repellency and mortality against Amblyomma americanum (Acari: Ixodida: Amblyommidae). J Med Entomol. 2016;58(4): 861–865. pmid:27113108
- 42. Murgia MV, Kaur J, Widder L, Hill CA. Efficacy of the transfluthrin-based personal insect protection kit (PIRK) against the ixodid ticks Ixodes scapularis, Amblyomma americanum, and Dermacentor variabilis. Current Research in Parasitology & Vector-Borne Diseases. 2022;2: 100070.
EthoVision XT (RRID:SCR_000441), Noldus Information Technology, Leesburg, VA, USA. Version 15.0 [software]. Available from: https://www.noldus.com/ethovision.
Altman DG. Practical statistics for medical research. Boca Raton (FL): CRC Press; 1990.
- 45. Sullivan GM, Feinn R. Using effect size–or why the p value is not enough. J Grad Med Educ. 2012;4(3): 279–82. pmid:23997866
Fleiss J. L. Measures of effect size for categorical data. In Cooper H.& Hedges L. V. The handbook of research synthesis. Russell Sage Foundation. 1994: 245–260.
SPSS Statistics for Windows (RRID:SCR_002865), Version 28.0 [software]. Armonk, NY: IBM Corp. Available from: htpps://www.ibm.com/spss/statistics.
- 48. Lang TA, Altman DG. Statistical analyses and methods in the published literature: The SAMPL guidelines. Medical Writing. 2016; 25(3): 31–35.
Cohen J. Statistical power analysis for the behavioral sciences (2nd ed.) Hillside, NJL Lawrence Erlbaum Associates, 1988.
- 50. Cohen J. A power primer. Psychol Bull. 1992;112: 155–159. pmid:19565683
- 51. Cummings P. Arguments for and against standardized mean differences (effect sizes). Arch Pediatr Adolesc Med. 2011;165(7): 592–596. pmid:21727271
GraphPad Prism (RRID:SCR_002798), Version 9.4.1 [software]. San Diego, CA: Dotmatics. Available from: htpps://www.graphpad.com.
Sonenshine DE, Roe RM. Biology of Ticks. 2nd ed. Oxford University Press; 2014.
- 54. Flor-Weiler LB, Behle RW, Eller FJ, Muturi EJ, Rooney AP. Repellency and toxicity of a CO2-derived cedarwood oil on hard tick species (Ixodidae). Exp appl Acarol. 2022;86: 299–312. pmid:35076809
- 55. Foster E, Fleshman AC, Ford SL, Levin ML, Delorey MJ, Eisen RJ, et al. Preliminary evaluation of human personal protection measures against the nymphal stage of the Asian Longhorned tick (Acari: Ixodidae). J Med Entomol. 2020;57(4): 1141–1148.
- 56. Carroll JF, Kramer M, Bedoukian RH. Solvent, drying time, and substrate affect the responses of Lone Star ticks (Acari: Ixodidae) to the repellents DEET and picardin. J Med Entomol. 2014;51(3): 629–637. pmid:24897855
- 57. Knap N, Durmisi E, Saksida A, Korva M, Petrovec M, Avsic-Zupanc T. Influence of climactic factors on dynamics of questing Ixodes ricinus ticks in Slovenia. Vet Parasitol. 2009;164(2–4): 274–281. pmid:19560275
- 58. Perret J-L, Guerin PM, Diehl PA, Vlimant M, Gern L. Darkness induces mobility, and saturation deficit limits questing duration, in the tick Ixodes ricinus. J Exp Biol. 2003;206(11): 1809–1815. pmid:12728002
- 59. Faraone N, MacPherson, Hiller NK. Behavioral responses of Ixodes scapularis tick to natural products: development of novel repellents. Exper Appl Acarol. 2019;79: 195–207.
- 60. Carr AL, Salgado VL. Ticks home in on body heat: A new understanding of Haller’s organ and repellent action. PLoS ONE. 2019;14(8): e0221659. pmid:31442282
- 61. Carr AL, Mitchell DR, Dhammi A, Bissinger BW, Sonenshine DE, Roe RM. Tick Haller’s Organ, a new paradigm for arthropod olfaction: how ticks differ from insects. Int J Mol Sci. 2017;18(7): 1563. pmid:28718821
- 62. Robertson HM. A forleg transcriptome for Ixodes scapularis ticks: candidates for chemoreceptors and binging proteins that might be expressed in the sensory Haller’s organ. Ticks Tick-borne Dis. 2018;9(5): 1317–1327. pmid:29886186
- 63. Faraone N, Light M, Scott C, MacPherson S, Hillier NK. Chemosensory and behavioural responses of Ixodes scapularis to natural products: role of chemosensory organs in volatile detection. Insects. 2020;11(8): 502.
- 64. Fryauff DJ, Shoukry MA, Schreck CE. Stimulation of attachment in a camel tick, Hyalomma dromedarii (Acari: Ixodidae): the unintended result of sublethal exposure to permethrin-impregnated fabric. J Med Entomol. 1994;31(1): 23–29. pmid:8158625
- 65. Ferreira LL, Gomes de Oliveira Filho J, Mascarin GM, Perez de Leon AA, Borges LMF. In vitro repellency of DEET and β- citronellol against the ticks Rhipicephalus sanguineus sensu lato and Amblyomma sculptum. Vet Parasitol. 2017;239: 42–45. pmid:28495195
- 66. Harmon JR, Scott MC, Baker EM, Jones CJ, Hickling GJ. Molecular identification of Ehrlichia species and host bloodmeal source in Amblyomma americanum L. from two locations in Tennessee, United States. Tick Tick-borne Dis. 105;6(3): 246–252. pmid:25682494
- 67. Kulma M, Kopecky O, Bubova T. Nymphs of Ixodes ricinus are more sensitive to Deet than adult females. J Am Mosq Control Assoc. 2019;35(4): 279–284. pmid:31922943
- 68. Benelli G. Pathogens manipulating tick behavior–through a glass, darkly. Pathogens. 2020;17(8): 664. pmid:32824571
- 69. Nevatte TM, Ward RD, Sedda L, Hamilton JGC, El Naiem D, Polwart A. Enhanced sandfly attraction to Leishmania-infected hosts. Trans R Soc Trop Med Hyg T Roy Soc Trop Med H. 2002;96(2): 117–118.
- 70. de la Fuente J, Villar M, Cabezas-Cruz A, Estrada-Peña A, Ayllón N, Alberdi P. Tick–host–pathogen interactions: conflict and cooperation. PLoS Pathog. 2016;12(4): e1005488. pmid:27099928
- 71. Faulde M.K, Robbins R.G. Tick infestation risk and Borrelia burgdorferi s.l. infection-induced increase in host-finding efficacy of female Ixodes ricinus under natural conditions. Exp Appl Acarol. 2008;44: 137–145. pmid:18273686
- 72. Belova OA, Burenkova LA, Karganova GG. Different tick-borne encephalitis virus (TBEV) prevalences in unfed versus partially engorged ixodid ticks–Evidence of virus replication and changes in tick behavior. Ticks Tick-borne Dis. 2012;3(4): 240–246. pmid:22910062
- 73. Eisen RJ, Eisen L. The blacklegged tick, Ixodes scapularis: an increasing public health concern. Trends Parasitol. 2018;34(4): 295–309. pmid:29336985
- 74. Fradin MS. Mosquitoes and mosquito repellents: a clinician’s guide. Ann Intern Med. 1998;128(11): 931–940. pmid:9634433