Halophyte Tamarix ramosissima. Lcdcb (T. ramosissima) are known as the representative of Tamarix plants that are widely planted in salinized soil. However, molecular mechanisms towards salt tolerance and adaptation are largely rare. In this study, we carried out RNA-sequence and transcriptome analysis of T. ramosissima in response to NaCl stress, screened differentially expressed genes (DEGs) and further verified by qRT-PCR. Results showed that 105702 unigenes were spliced from the raw data of transcriptome sequencing, where 54238 unigenes were retrieved from KEGG, KOG, NR, and SwissProt. After 48 hours of NaCl treatment, the expression levels of 6374 genes were increased, and 5380 genes were decreased in leaves. After 168 hours, the expression levels of 3837 genes were up-regulated and 7808 genes were down-regulated. In particular, 8 transcription factors annotated to the KEGG Pathway were obtained, involving the WRKY and bZIP transcription family. In addition, KEGG pathway annotation showed that expression of 39 genes involved in ROS scavenging mechanisms were significantly changed, in which 21 genes were up-regulated and 18 genes were down-regulated after 48 hours as well as 15 genes were up-regulated and 24 genes were down-regulated after 168h. Simultaneously, the enzyme activities of SOD and POD were significantly enhanced under NaCl treatment, but the enzyme activity of CAT was not significantly enhanced. Moreover, WRKY, MYB and bZIP may participate in the process of salt resistance in T. ramosissima. This study provides gene resources and a theoretical basis for further molecular mechanisms of salt tolerance in T. ramosissima.
Citation: Chen Y, Wang G, Zhang H, Zhang N, Jiang J, Song Z (2022) Transcriptome analysis of Tamarix ramosissima leaves in response to NaCl stress. PLoS ONE 17(3): e0265653. https://doi.org/10.1371/journal.pone.0265653
Editor: Guangxiao Yang, Huazhong University of Science and Technology, CHINA
Received: December 9, 2021; Accepted: March 4, 2022; Published: March 31, 2022
Copyright: © 2022 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting information files.
Funding: This work was jointly supported by the following grants: the Agricultural Variety Improvement Project of Shandong Province (2019LZGC009), China’s National Science Foundation through grants (32071612), and the China Scholarship Council (202108320311). Agricultural Variety Improvement Project of Shandong Province provided experimental materials (Hongxia Zhang). China’s National Science Foundation through grants (Jiang Jiang)and the China Scholarship Council provide test sites and data collection(Yahui Chen). Agricultural Variety Improvement Project of Shandong Province, China’s National Science Foundation through grants and the China Scholarship Council provided great help in research design, data collection and analysis.
Competing interests: NO authors have competing interests.
Salinized soil has high salt content and poor soil physical and chemical properties, which seriously hindered the growth and development of plants . Salinized soil contains a lot of Na+ type salt which can destroy the stability of protein and membrane, and produces osmotic stress and ion poisoning to initiate reactive oxygen ROS (reactive oxygen species) signals in the cell, make dysfunction of the cell, affect the growth of plants, and causes plant death in severe cases . In the past decades, the area of salinized soil has continued to expand due to global human activities and climate changes. Favorably, it is becoming a hotspot to carry out afforestation, restore salinized soil and improve the ecological environment in salinized soil areas.
In recent years, RNA-Seq technology has been widely used to study molecular mechanisms of plant resistance to adverse stresses, including salt stress [3, 4]. Previous studies showed that reactive oxygen species (ROS) would be produced in multiple cell compartments, including chloroplasts , mitochondria , and peroxisomes  under salt stress conditions. A relatively low concentration of ROS is known as an important signal molecule to regulate the normal plant growth and responses to abiotic stresses [8, 9]. However, excessive accumulation of ROS can adversely cause cell oxidative damage . To adapt to ROS damage, higher plants have evolved corresponding regulatory mechanisms to maintain the stability of life activities. Notably, ROS scavenging enzymatic systems, such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione peroxidase (GPX), are prone to play important roles to adapt to undesired abiotic stresses . In addition, plants can also take advantage of the ascorbic acid- reduced glutathione (AsA-GSH) cycle, which contains APX, glutathione reductase (GR) and AsA, and GSH, and so on, to eliminate the damage of ROS .
In particular, transcription factors are the most important regulators for plants to respond to various abiotic stresses . In details, reports are focused on the involvement of transcription factors of WRKY , bHLH , bZIP  NAC , MYB , AP2/ERF  under salt stress. In cotton, the WRKY transcription factor gene GhWRKY34 was induced by salt stress in transgenic Arabidopsis . In Paspalum notatum, the WRKY transcription factor genes are involved in regulating the expression of SOD and related oxidoreductase genes to adapt to salt stress . Notably, Tamarix plants have evolved a complex regulatory network for a long time to adapt to the adverse abiotic stresses . T. hispida is often used as a biological material to explore the molecular mechanisms towards salt stress tolerance. Overexpression of the T. hispida ThCOL2 gene can regulate the activity of protective enzymes and reduce the accumulation of O2− and H2O2 that enhanced the ROS scavenging ability and improved the adaptability of transgenic T. hispida under salt stress . Overexpression of ThbZIP1 enhanced the activity of POD and SOD, increased the content of soluble sugar and soluble protein that further improved salt tolerance in transgenic T. hispida . T. ramosissima usually grows in arid and semi-arid regions with high salt content . Moreover, low concentration (<100mM) NaCl promoted while high concentration (≥200mM) NaCl stress inhibited the growth of T. Ramosissima . Liu and her colleagues found that T. ramosissima exhibited the strongest salt tolerance among 3 Tamarix species, including Tamarix gansuensis H.Z.Zhang, Tamarix leptostachys Bunge, and Tamarix ramosissima. Lcdcb, and T. ramosissima were chosen as the representative species of Tamarix plants for further mechanisms studies.
In this study, we performed high-throughput transcriptome sequencing in T. ramosissima under NaCl stress and screened and verified DEGS at the transcriptional level. This study lays a theoretical basis to reveal molecular mechanisms towards salt tolerance in Tamarix plants, and provides gene resources for further variety breeding of salt-tolerant Tamarix plants.
2. Materials and methods
2.1. Plant materials
T. ramosissima seedlings were provided by the Dongying Experimental Station of Shandong Academy of Forestry Sciences. Experiments were completed at the Key Laboratory of Forest Tree Genetic Breeding and Biotechnology of the Ministry of Education of Nanjing Forestry University from October 2019 to March 2021. 5-month-old T. ramosissima seedlings with uniform growth were transferred to a 24-well hydroponic box (size: 40cm*30cm*16cm), supplemented with 1/2 Hoagland nutrient solution, and then placed in a greenhouse that was maintained at 26 ± 2°C (day) whose relative humidity stays between 40% and 55% for 1 month after training before treatment. The culture solution was changed every 3rd day.
2.2 NaCl treatment
In the control group (CK), seedlings were suffered with 1/2 Hoagland nutrient solution. In the treatment group, seedlings were cultured in 1/2 Hoagland nutrient solution, supplemented with 200 mM NaCl. 8 plants were used in each group, and the experiments were repeated 3 times. The culture solution was changed every 3rd day. The leaf samples were collected at 0h, 48h, and 168h, respectively, and immediately frozen in liquid nitrogen, and then moved to a -80°C refrigerator for storage.
2.3 Phenotype and antioxidative enzyme activity analysis in T. ramosissima leaves
Leaves of T. ramosissima were collected after 0h, 48h and 168h of NaCl treatment, respectively, and the distribution of salt secretion on the leaf surface was observed using a JSZ6S stereo microscope (Jiangnan, China). The activities of SOD , POD  and CAT  were determined and analyzed, according to the description of the commercial Extraction Kits (Jiancheng Limit Co., Nanjing, China).
2.4 Transcriptome sequencing
The frozen leaf samples were used for 3-generation high-throughput transcriptome sequencing, using Illumina HiSeq™4000, in Guangzhou GENE Denovo Company. The purified PCR products were analyzed by pair-end sequencing (PE150) on the platform according to standard operations, and then fastp was used for quality control . The original data was first filtered to obtain clean reads, then assembled . These assembled fragments without N terminal obtained by reading overlap are used as the assembled Unigene, and then use Blast2 GO  and KOBAS  to obtain Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation. The Illumina raw sequencing data were submitted to the National Center for Biotechnology Information (NCBI) Short Reads Archive (SRA) database under accession number SRP356215.
2.5 Screening methods for differentially expressed genes
DESeq2 software was used to analyze the reads count data to obtain the final correct FDR value (FDR value means BH corrected p value) . A corrected p value of <0.05 is considered to be significantly enriched. Based on the results of the difference analysis, genes with FDR value <0.05 and |log2FC| > 1 are considered to be significantly different genes.
2.6 Quantitative Real-Time PCR (qRT-PCR) validation of DEGs
Eight putative genes (Unigene0104732, Unigene0028215, Unigene0083695, Unigene0069097, Unigene0090596, Unigene0024962, Unigene0007135 and Unigene0088781) were randomly selected to verify the accuracy of the RNA-Seq results by the qRT-PCR technique. The total RNA was extracted with Omega RNA Extraction Kit (Shanghai, China), and then reverse-transcribed the 1-strand cDNA by using the PrimerScript™ RT Master Mix Kit (TaKaRa, Dalian, China). Specific primers of DEGs are designed via the Primer-BLAST server (S1 Table). qRT-PCR samples were labelled with PowerUp™ SYBR Green Master mix reagent (Thermo Fisher, China) and then performed on ABI ViiA™ 7 Real-time PCR system (ABI, USA). A total of 3 biological repeats were performed, each with 4 technical repetitions. Actin was used as the internal reference gene, and the relative expression level was calculated by the 2−ΔΔCt method.
3.1 Phenotype and antioxidative enzyme activity analysis in T. ramosissima leaves
In the CK group, there was no salt secretion in the leaves of T. ramosissima at 0h, 48h and 168h. However, leaves began to excrete a small amount of salt at 48h under 200 mM NaCl treatment, and the salt secretion reached the maximum amount at 168h under NaCl treatment (Fig 1). These findings showed that the amount of salt secretion in leaves increased along with the prolongation of NaCl treatment time.
[Leaf salt secretion are checked in the leaves of T. ramosissima at 0h, 48h and 168h under 200mM NaCl treatment (The red labels indicate the salt secretion products)].
The activities of SOD, POD and CAT in the leaves of T. ramosissima showed an increasing trend under 200mM NaCl for 48h and 168h, compared with the control group. In particular, the activities of SOD and POD were significantly higher than those of the control group after either 48h or 168h. The CAT activity increased slightly but had no significant change, compared with the control group (Fig 2). These results showed that SOD and POD activities under salt stress in the leaves of T. ramosissima.
3.2 Sequencing quality analysis
Using IlluminaHiSeq™4000, obtained multiple high-quality bases at 0h, 48h and 168h were obtained in T. ramosissima leaves under 200mM NaCl stress, and Q20 reached more than 95%, and Q30 reached more than 90% and the GC content is above 44% (Table 1), indicating that the quality of the transcriptome sequencing is relatively high, which is reliable for further analysis.
3.3 Unigene basic notes
Results showed that a total of 105,702 Unigenes were spliced in this study. There are 53,385, 46062, 31587, and 36087 Unigenes with gene annotations in the Nr, KEGG, KOG, and SwissProt databases, respectively, and a total of 27,670 Unigenes were simultaneously screened with annotations in the four major databases (Fig 3).
3.4 Quantitative expression analysis of DEGs
Taking the CK group as the control, the transcription data of T. ramosissima under NaCl stress treatment at 0h, 48h and 168h were compared and analyzed, respectively. DEGs were screened with the standard of FDR value <0.05, p value <0.05 and |log2FC| > 1 after correction. In the CK-0h v.s. NaCl-48h comparison group, a total of 11,754 gene expression levels were detected, in which 6374 genes were up-regulated and 5380 genes were down-regulated under NaCl treatment. In the CK-0h v.s. NaCl-168h comparison group, a total of 7768 gene expression changes were detected, in which 2542 genes were induced and 5226 genes were reduced under NaCl treatment (Fig 4).
3.5 GO analysis of DEGs
Through GO annotation analysis, the above-mentioned DEGs can be divided into 3 categories: biological processes, cellular components, and molecular functions, and a total of 51 different classification groups were observed (Fig 5). In detail, in the CK-0h v.s. NaCl-48h comparison group, the up-regulated genes were slightly more than the down-regulated genes, while in the CK-0h v.s. NaCl-168h comparison group, the down-regulated genes were significantly more than the up-regulated genes. In the broad category of biological process, DEGs are mainly concentrated in cellular process, metabolic process, single-organism process and response to stimulus (Table 2). In the molecular function category, DEGs are mainly concentrated in catalytic activity, binding, transporter activity and structural molecule activity (Table 3). Among the major categories of cell components, DEGs are mainly enriched in cell, cell part, organelle and membrane (Table 4). In addition, the number of up-regulated DEGs was significantly lower and the overall number of DEGs decreased, along with the time of NaCl treatment. We speculate that T. ramosissima leaves may respond to high NaCl stress by affecting the expression level of related DEGs at the transcription level.
(The first and outer circle: the top 20 GO terms are enriched, outside the circle is the scale of the number of genes. Different colors represent different Ontologies. The second circle: the number of the GO term in the background gene and the Q value. The more genes, the better the bars. Long, the smaller the Q value, the darker the color. The third circle: the bar graph of the ratio of up-regulated genes, the dark color represents the proportion of up-regulated genes, and the light color represents the proportion of down-regulated genes. The specific value is displayed below. The fourth and inner circle: the ratio of each GO term Rich Factor value (number of differential genes in this GO term divided by all numbers), background grid lines, each grid represents 0.1).
3.6 KEGG pathway analysis of DEGs in T. ramosissima leaves under NaCl stress KEGG pathway analysis showed that 1762 and 1366 DEGs were annotated in the comparison group of CK-0h v.s. NaCl-48h and CK-0h v.s. NaCl-168h, respectively (Fig 6), which directly reflected the changes of gene expression in leaves of T. ramosissima under NaCl stress. Among the top 10 KEGG pathways from the CK-0h v.s. NaCl-48h comparison group, Ribosome (ko03010) annotated to 435 DEGs, accounting for 24.69%, followed by biosynthesis of secondary metabolites (ko01110), phytopathogen interaction (ko04626), phenylpropane biosynthesis (ko00940), plant hormone signal transduction (ko04075) and plant MAPK signaling pathway (ko04016), respectively, annotated to 416 (23.61%), 88 (4.99%), 69 (3.92%), 69 (3.92%) and 59 3.35% DEGs. Among the top 20 pathways from the CK-0h v.s. NaCl-168h comparison group, the metabolic pathway (ko1100) was annotated to 614 DEGs, accounting for 44.95%, followed by the Biosynthesis of secondary metabolites (ko01110), Ribosomes (ko03010), Oxidative phosphorylation (ko00190), Plant hormone signal transduction (ko04075) and Plant pathogen interaction (ko04626), respectively, annotated to 351 (25.70%), 296 (21.67%), 104(7.61%), 72 (5.27%) and 63 (4.61%) DEGs. In summary, DEGs are significantly enriched on the KEGG pathway, such as metabolic pathways, biosynthesis of secondary metabolites, and plant hormone signal transduction, in T. ramosissima under NaCl treatment.
3.6 Analysis of antioxidant DEGs in T. ramosissima leaves under NaCl stress
Results showed that the expression of 39 ROS scavenging-related DEGs in the leaves of T. ramosissima were changed under salt treatment (Table 5). Notably, there were 21 up-regulated genes and 18 down-regulated genes from the CK-0h v.s. NaCl-48h comparison group. The largest number of up-regulated genes is GST (7), followed by POD (4), CAT (4), APX (3), GR (2) and SOD (1). The largest number of down-regulated genes is GST (8), followed by POD (4), SOD (3), APX (2) and GPX (1). In the CK-0h v.s. NaCl-168h comparison group, there were 15 up-regulated genes and 24 down-regulated genes. In particular, the largest number of up-regulated genes are CAT(3), APX(3) and GST(3), followed by SOD(2), POD(2), and GR(2). The largest number of down-regulated genes is GST(12), followed by POD(6), SOD(2), APX(2), CAT(1) and GPX(1) (Fig 7). These results showed implying that the antioxidant mechanism might be initially enhanced in T. ramosissima leaves accompanied with corresponding physiological responses to resist NaCl stress during the first 48 hours. However, the antioxidant mechanism might be initially inhibited in T. ramosissima leaves under a long time (168 h) of NaCl treatment, just to adapt to salt stress.
(Notes: GR, glutathione reductase; GST, glutathione S-transferases; APX, ascorbate peroxidase; GPX, glutathione peroxidase; CAT, Catalases; POD, peroxidase; SOD, Superoxide dismutase. The number of up-regulated and down-regulated activities of each enzyme in the reactive oxygen species scavenging mechanism shown in the figure in the comparison of CK V.S. NaCl-48h and CK V.S. NaCl-168h).
3.7 Analysis of transcription factor DEGs in T. ramosissima leaves under NaCl stress
Transcription factors play a major role in regulating plant growth and adaptation to adverse environments, including salt stress. In this study, According to the transcriptome sequencing of T. ramosissima leaves, many transcription factors were discovered. In this study, we did select 8 statically significant DEGs, which were specifically observed and annotated in the KEGG database. In particular, 5 WRKY (ko04626 and ko04016) annotated genes were responsive to NaCl treatment. In particular, the expression levels of Unigene0010090, Unigene0077293 and Unigene0079542 exhibited a downward trend at 0h, 48h and then an upward trend at 168h. The expression level of Unigene0014406 and Unigene0024962 continuously increased until 168h. In addition, 3 bZIP transcription factors were annotated to the KEGG pathway (ko04016, ko01100, ko01110, ko01200, ko01212, ko04146, ko00071, ko00640, ko00410, ko01040, ko00592 and ko04075). The expression level of Unigene0026888 and Unigene0008868 exhibited a downward trend at 48h and then an upward trend at 168h, while Unigene0010561 showed a downward trend at 48h and then an upward trend at 168h (Table 6).
3.8 Quantitative Real-Time PCR (qRT-PCR) validation of differential expression
We further randomly selected 8 DEGs involved in salt stress for qRT-PCR verification (S2 Table). Results showed that the expression level of Unigene0104732, Unigene0083695 and Unigene0069097 were induced at 48h but reduced at 168h, while genes of Unigene0090596, Unigene0007135 and Unigene0088781 were decreased at 48h but increased at 168h. Notably, Unigene0024962 was continuously increased while Unigene0028215 was continuously decreased under NaCl stress. These qRT-PCR verification results are completely consistent with the expression trends observed from the transcriptome sequencing analysis (S1 Fig). Nonetheless, the transcriptome data obtained in this study is accurate and reliable.
Tamarix plants have evolved a series of complex mechanisms to resist and adapt to salt stress in the long term. In particular, Tamarix plants have typical multicellular salt glands, which can reduce plant damage from ion poisoning, which is one of the important morphological characteristics of Tamarix plants to adapt to the saline environment . In this study, electron microscopy analysis showed that leaf salt secretion increased with the prolongation of NaCl treatment, suggesting that T. tamariska may alleviate and respond to the toxicity of salt stress via the salt secretion pathway, thereby adapting to the adverse long-term salt stresses.
The response of plants under salt stress is quite complex that needs multiple gene families and regulatory mechanisms involved in many biological pathways, including metabolism, signal transduction, energy production and transportation, ion penetration and transportation . A comprehensive transcriptome analysis of T. ramosissima plants helps to reveal the molecular mechanisms of Tamarix plants in response to salt stress.
4.1 Enhanced active oxygen scavenging capacity in T. ramosissima
Plants are usually affected by various unfavorable environmental factors, including salt stress, that results in a large amount of ROS accumulation. In this present study, 39 ROS scavenging-related DEGs were significantly regulated under NaCl stress, implying that ROS scavenging mechanisms were indispensable for T. ramosissima plants under NaCl stress.
SOD, POD and CAT enzymes are involved in ROS scavenging in plant cells. Under abiotic stresses, expression of SOD, POD and CAT related genes are prone to be up-regulated . Notably, the expression level of the TaSOD1.7 gene in leaves increased significantly, and the salt tolerance of transgenic wheat was enhanced . In this study, 1 SOD, 4 POD and 4 CAT related genes were induced, while 3 SOD and 4 POD related genes are down-regulated at 48 h of NaCl treatment. Even168 hours after finishing NaCl treatment, there are still 2 SOD, 2 POD and 3 CAT related genes were up-regulated and 2 SOD, 6 POD and 1 CAT related genes were down-regulated. Together, these genes mentioned above may contribute to the increased enzyme activities of SOD and POD in T. ramosissima leaves under NaCl treatment.
Both APX and GPX are the key enzymes for enzymatically removing ROS, protecting cells by catalyzing H2O2, and playing an important role in plant adversity stress. APX family genes are involved in plant tolerance to drought, heat, salt, oxidation and biological stresses . In this study, the expression of 3 APX related genes was up-regulated and 2 genes were down-regulated under NaCl treatment. However, only one GPX related gene was down-regulated. We guess that APX and GPX genes in T. ramosissima leaves may respond to salt stress in different ways.
Glutathione S-transferase (GST) is involved in detoxification and antioxidant defence, protecting plants from different abiotic stresses and adversities, and playing multiple roles in plant growth and development [39–42]. In particular, GST was involved in salt stress and GST-related genes are non-sensitive to low and medium NaCl (≤100 mM) concentration but are sensitive to high NaCl (≥200 mM) stress [39, 46]. In this study, 3 GST related genes were up-regulated and 8 genes were down-regulated in the leaves at 48 h, and 3 genes were up-regulated and 12 genes were down-regulated at 168 h, implying that there may exist both positive and negative regulation of GST in T. ramosissima under NaCl stress, and the negative regulation may be the dominant.
GR is one of the plant antioxidant enzymes and a flavoprotein oxidoreductase, which exists in eukaryotes and prokaryotes, and plays an important role in the elimination of ROS in the process of plant oxidative stress . The expression of Oryza sativa GR3  and Jatropha JcGR  are all up-regulated under salt stress. Similar to the previous studies, two GR related genes were up-regulated in T. ramosissima leaves under NaCl stress. We speculate that GR might be involved in the adaptation of T. ramosissima to NaCl stress in a positive direction.
4.2 Responsive transcription factors under NaCl treatment ins T. ramosissima
Transcription factors are indispensable for plants to respond to abiotic stresses . WRKY is one of the most important transcription factor families, which has been verified to participate in a variety of metabolic processes and plays an important role in the regulation of transcriptional reprogramming related to plant biological and abiotic stress responses [46–48]. In this study, Unigene0024962, which encodes a WRKY transcription factor, was up-regulated by NaCl treatment, while Unigene0079542 was decreased at 48 h but increased at 168 h, which is consistent with the previous reports in WRKY family genes in soybean  and Reaumuria trigyna . The expression of CaWRKY27 in pepper was down-regulated by NaCl stress , which is in contrast to our findings. Notably, Unigene0014406 was continuously induced by NaCl treatment, suggesting that this gene may be inevitably involved in the response of T. ramosissima to salt stress. In addition, bZIP transcription factors play an important regulatory role in plant growth and environmental stress response [52, 53]. The expression of Unigene0008868 was significantly up-regulated by NaCl treatment, which is consistent with that of Huang’s findings in ramie, implying that the bZIP transcription factor plays an important regulatory role in response to salt stress . Moreover, MYB is one of the most diversified transcription factor families in plants in terms of quantity and function, which plays important roles under abiotic stresses [55, 56]. In this study, the expression level of Unigene0088781 decreased in the first 48 hours and then increased in a long time under NaCl treatment, suggesting that this gene may be active, especially during a specific period under salt stress. Similar findings were also observed in Medicago sativa seedlings  and Rosa rugosa petals .
The raw data of transcriptome sequencing of T. ramosissima leaves in response to NaCl stress was spliced into 105702 Unigenes, and 54238 annotated Unigenes were retrieved in the 4 major functional databases of KEGG, KOG, NR and SwissProt. The expression profiles of DEGs are slightly different between short time (48h) and long time (168) treatments. In particular, ROS scavenging genes and transcription factor encoding genes (including WRKY, MYB and bZIP family) are sensitive to NaCl treatment with distinct regulatory statuses. This study provides the theoretical basis and gene resource for further molecular mechanisms towards salt tolerance in T. ramosissima.
This work was jointly supported by the following grants: the Agricultural Variety Improvement Project of Shandong Province (2019LZGC009).
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