The performance of the real-time RT-PCR assay for PRV was based on published protocols (the assay used primers and probe by Haugland et al., 2011, Journal of Virology 85:5275–5286, and reaction conditions by Palacios et al., 2010. PLoS ONE 5:e11487). A cut-off Ct value of 40 was used as established by Løvoll et al., 2012. Ct values ≤ 40 were considered positive.
The Ct values in this study were automatically reported by the real-time instrument (LC 480, Roche), which reported Ct values above 40 as 0. Thus, any samples generating Ct values that were > 40 were designated as negative along with samples for which no Ct value was generated.
Minor transcription errors in the file ‘S3_Table Wild Fish Data" have been corrected in the revised version of this file attached to this correction notice. We make the following minor corrections: (i) The percentage of positive tests in the farmed Atlantic salmon sampled fish should be lowered from 95% to 93%, and (ii) the percentage of positive tests in the farmed steelhead sampled fish should be raised from 69% to 71%. These minor corrections had no impact on the results of the statistical analyses reported in the paper or the subsequent discussion or conclusions.
- 1. Morton A, Routledge R, Hrushowy S, Kibenge M, Kibenge F (2017) The effect of exposure to farmed salmon on piscine orthoreovirus infection and fitness in wild Pacific salmon in British Columbia, Canada. PLoS ONE 12(12): e0188793. https://doi.org/10.1371/journal.pone.0188793 pmid:29236731
- 2. Haugland O, Mikalsen AB, Nilsen P, Lindmo K, Thu BJ, Eliassen TM, Roos N, Rode M, Evensen O (2011) Cardiomyopathy Syndrome of Atlantic Salmon (Salmo salar L.) Is Caused by a Double-Stranded RNA Virus of the Totiviridae Family. Journal of Virology 85:5275–5286. https://jvi.asm.org/content/85/11/5275 pmid:21411528
- 3. Palacios G, Lovoll M, Tengs T, Hornig M, Hutchison S, Hui J, et al. . . . Heart and skeletal muscle inflammation of farmed salmon is associated with infection with a novel reovirus. PLoS ONE 5(7): e11487. https://doi.org/10.1371/journal.pone.0011487 pmid:20634888
- 4. Løvoll M, Alarcón M, Jensen BB, Taksdaln T, Kristoffersen AB, Tengs T (2012) Quantification of piscine reovirus (PRV) at different stages of Atlantic salmon Salmo salar production. Diseases of Aquatic Organisms Vol. 99: 7–12, 2012. pmid:22585298
Citation: Morton A, Routledge R, Hrushowy S, Kibenge M, Kibenge F (2021) Correction: The effect of exposure to farmed salmon on piscine orthoreovirus infection and fitness in wild Pacific salmon in British Columbia, Canada. PLoS ONE 16(3): e0248912. https://doi.org/10.1371/journal.pone.0248912
Published: March 16, 2021
Copyright: © 2021 Morton et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.