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Maternal plasma levels of oxytocin during breastfeeding—A systematic review

  • Kerstin Uvnäs­Moberg,

    Roles Conceptualization, Funding acquisition, Methodology, Project administration, Supervision, Validation, Writing – original draft, Writing – review & editing

    Affiliation Department of Animal Environment and Health, Swedish University of Agricultural Sciences, Skara, Sweden

  • Anette Ekström-Bergström ,

    Roles Data curation, Formal analysis, Methodology, Project administration, Supervision, Writing – original draft, Writing – review & editing

    Affiliation Department of Health Sciences, University of West, Trollhättan, Sweden

  • Sarah Buckley,

    Roles Formal analysis, Methodology, Project administration, Writing – original draft, Writing – review & editing

    Affiliation School of Public Health, The University of Queensland, Herston, QLD, Australia

  • Claudia Massarotti,

    Roles Formal analysis, Writing – review & editing

    Affiliations Academic Unit of Obstetrics and Gynecology, University of Genova, Genova, Italy, Physiopathology of Human Reproduction Unit, IRCCS Ospedale Policlinico San Martino, Genova, Italy

  • Zada Pajalic,

    Roles Data curation, Formal analysis, Writing – review & editing

    Affiliation Faculty of Health Studies, Campus Diakonhjemmet, VID Specialized University, Oslo, Norway

  • Karolina Luegmair,

    Roles Conceptualization, Data curation, Writing – review & editing

    Affiliation Berufs Bildung Zentrum Gesundheit Ingolstadt, Ingolstadt, Germany

  • Alicia Kotlowska,

    Roles Data curation, Writing – review & editing

    Affiliation Department of Clinical & Experimental Endocrinology, Faculty of Health Sciences with Subfaculty of Nursing and Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Gdańsk, Poland

  • Luise Lengler,

    Roles Formal analysis, Writing – review & editing

    Affiliation Midwifery Education Unit, Freiburg University Medical Center, Freiburg, Germany

  • Ibone Olza,

    Roles Formal analysis, Writing – review & editing

    Affiliation Faculty of Medicine, University of Alcalá, Alcalá de Henares, Spain

  • Susanne Grylka-Baeschlin,

    Roles Formal analysis, Writing – review & editing

    Affiliation Research Unit for Midwifery Science, Zurich University of Applied Sciences, Winterthur, Switzerland

  • Patricia Leahy-Warren,

    Roles Formal analysis, Writing – review & editing

    Affiliation School of Nursing and Midwifery, University College Cork, Cork, Ireland

  • Eleni Hadjigeorgiu,

    Roles Formal analysis, Writing – review & editing

    Affiliation Nursing Department, Health Science, Cyprus University of Technology, Limassol, Cyprus

  • Stella Villarmea,

    Roles Formal analysis, Writing – review & editing

    Affiliations Faculty of Philosophy, University of Alcalá, Alcalá de Henares, Spain, Faculty of Philosophy, University of Oxford, Oxford, United Kingdom

  • Anna Dencker

    Roles Data curation, Formal analysis, Methodology, Writing – review & editing

    Affiliation Institute of Health and Care Sciences, Sahlgrenska Academy, University of Gothenburg, Göteborg, Sweden



Oxytocin is a key hormone in breastfeeding. No recent review on plasma levels of oxytocin in response to breastfeeding is available.

Materials and methods

Systematic literature searches on breastfeeding induced oxytocin levels were conducted 2017 and 2019 in PubMed, Scopus, CINAHL, and PsycINFO. Data on oxytocin linked effects and effects of medical interventions were included if available.


We found 29 articles that met the inclusion criteria. All studies had an exploratory design and included 601 women. Data were extracted from the articles and summarised in tables. Breastfeeding induced an immediate and short lasting (20 minutes) release of oxytocin. The release was pulsatile early postpartum (5 pulses/10 minutes) and coalesced into a more protracted rise as lactation proceeded. Oxytocin levels were higher in multiparous versus primiparous women. The number of oxytocin pulses during early breastfeeding was associated with greater milk yield and longer duration of lactation and was reduced by stress. Breastfeeding-induced oxytocin release was associated with elevated prolactin levels; lowered ACTH and cortisol (stress hormones) and somatostatin (a gastrointestinal hormone) levels; enhanced sociability; and reduced anxiety, suggesting that oxytocin induces physiological and psychological adaptations in the mother. Mechanical breast pumping, but not bottle-feeding was associated with oxytocin and prolactin release and decreased stress levels. Emergency caesarean section reduced oxytocin and prolactin release in response to breastfeeding and also maternal mental adaptations. Epidural analgesia reduced prolactin and mental adaptation, whereas infusions of synthetic oxytocin increased prolactin and mental adaptation. Oxytocin infusion also restored negative effects induced by caesarean section and epidural analgesia.


Oxytocin is released in response to breastfeeding to cause milk ejection, and to induce physiological changes to promote milk production and psychological adaptations to facilitate motherhood. Stress and medical interventions during birth may influence these effects and thereby adversely affect the initiation of breastfeeding.


The European Cooperation in Science and Technology (EU COST), supports scientific collaboration within the European Union. This article was created within the framework of the COST Action IS1405 BIRTH: "Building Intrapartum Research Through Health—An interdisciplinary whole system approach to understanding and contextualising physiological labour and birth" ( A literature review article regarding plasma levels of oxytocin in connection with labour and birth, performed within this COST action has already been published [1].

Breastfeeding is recognized as the best way to feed infants and the World Health Organization (WHO) recommends exclusive breastfeeding for the first six months of life [2, 3]. Breastfeeding gives rise to many positive effects in babies and their mothers. It for example protects against infections in new-borns and early in life and it may also reduce the occurrence of some allergic conditions, obesity, and type 1 diabetes later on in childhood. In addition, it has also been suggested to increase the level of intelligence [4]. For mothers, breastfeeding stimulates physiological and psychological changes in order to facilitate milk production and adaptations to motherhood. In addition, it gives long-term protection against diseases such as breast cancer, ovarian cancer, cardiovascular disease and diabetes type 2 [4, 5].

Breastfeeding also supports the interaction and bonding between mother and infant. Longer duration of breastfeeding was associated with more maternal sensitive responsiveness and more attachment security and less attachment disorganization in the child [6].

Oxytocin, a peptide molecule produced in the supraoptic and paraventricular nuclei (SON and PVN) of the hypothalamus, plays a key role during breastfeeding. Oxytocin released into the circulation during breastfeeding promotes milk ejection by contracting the myoepithelial cells surrounding the mammary gland alveoli and by relaxing the milk duct sphincters.

Suckling also induces a release of oxytocin from nerves within the brain where oxytocin facilitates both physiological and psychological adaptations for breastfeeding and motherhood. Oxytocin promotes prolactin release and thereby milk production. It also induces powerful anti-stress effects, including decreased blood pressure and cortisol levels, and stimulates digestive and metabolic processes [7].

The release of oxytocin into the circulation and the brain occurs in parallel in response to each breastfeeding episode. A recent publication provides an elaborate description of the mechanisms by which the release of oxytocin into the circulation and brain are coordinated [1].

It has been suggested that medical interventions during labour and birth, including caesarean section [8], epidural analgesia [9], and infusion of synthetic oxytocin [10] might negatively impact the initiation and/or continuation of breastfeeding. Medical interventions may hypothetically cause such effects by influencing the release of and effects caused by oxytocin during labour and birth in the long term.

The aim of this study was to review plasma levels of oxytocin levels in response to breastfeeding. Also, other oxytocin linked effects presented in the identified articles as well as data regarding effects of medical interventions during labour and birth are reported. Data obtained within the same article allows more detailed investigations of the relationship between oxytocin and oxytocin linked effects, than does data presented in separate articles. The same applies for articles on the effects of medical interventions.


A systematic literature search, with the aim to summarize the knowledge regarding oxytocin levels in response to breastfeeding, was carried out according to the PRISMA statement [11] and using Covidence©, an online platform for systematic reviews [12]. The main inclusion criterion was plasma levels of oxytocin in response to breastfeeding, All the inclusion criteria are shown in Table 1.

The search strings were created by KUM, AEB and SB together with librarians from the University of Queensland and searches were performed in the following databases: Pubmed, Scopus, CINAHL, and PsycINFO in September 2017. An additional literature search based on the same search string was performed in September 2019.

Procedure for selection of studies

In 2017, 453 articles were identified via database searches (PubMed n = 305, Scopus n = 91, CINAHL n = 7, and PsycINFO n = 50). Fifteen additional articles were identified through other sources. Forty duplicates were removed from the 468 articles leaving 428 articles. By screening of titles and abstracts, 268 of the 468 articles were excluded. After full-text screening of the remaining 160 articles, 28 articles were identified that met the inclusion criteria, were included in this review. A last search was performed in September 2019. Additional 34 papers were identified. After removing duplicates and screening of titles and abstracts, two full text papers were included and reviewed. One paper was excluded due to no reporting of oxytocin levels [13] and one paper met the inclusion/exclusion criteria [14].

All together 29 articles were identified in which maternal oxytocin levels were recorded during breastfeeding or other types of breast stimulation [1442]. Note that the 29 included articles are based on 25 clinical studies, as data from 3 of the clinical studies had been split and published in 2 separate articles, thus [20, 34] and [30, 32] and [36, 37] were based on the same clinical studies. For 2 of the other included articles, Jonas et. al. 2009 [24] and Uvnäs Moberg et. al. 1990 [37] additional material regarding breastfeeding induced effects had been published as separate articles [4345] and [46, 47] respectively. Data from these additional studies have also been included in the tables. Altogether 601 women participated in the studies summarized.

At each stage, articles were screened by at least two authors working independently in pairs (AEB, KUM, CM, KL, SB & ZP) based on the inclusion and exclusion criteria (Table 1). In case inclusion was unclear, an expert third author (KUM) was involved. Final decisions were agreed by SB & KUM. The selection process is illustrated in a flow diagram (Fig 1).

Procedure for data extraction and compilation of data

The articles included in this study are mainly of an explorative research design and are not randomized, controlled, intervention studies. Data were extracted from the articles and compiled into tables. KUM, SB, AEB, AK, ZP, LL and KL participated in this work. Characteristics of the included articles are presented in Table 2, data on oxytocin levels in Tables 3 and 4, prolactin levels in Table 5, variables related to stress in Table 6, variables related to digestion and nutrition in Table 7 and variables related to personality and mood in Table 8. If data on associations between oxytocin and the secondary effects of oxytocin was available, these data were also included in the tables. Data on effects of medical interventions on breastfeeding induced oxytocin levels are presented separately (Table 4).

Table 2. Characteristics of the included clinical studies.

Table 3. Oxytocin levels during breastfeeding or other types of breast stimulation.

Table 4. Oxytocin levels during breastfeeding or other types of /breast stimulation, women with medical interventions during labour, birth or postpartum.

Table 5. Prolactin levels during breastfeeding or other types of /breast stimulation, including women with medical interventions during labour, birth or postpartum.

Table 6. Stress indicators during breastfeeding or other types of /breast stimulation, including women with medical interventions during labour, birth or postpartum.

Table 7. Nutritional and metabolic variables in connection with breastfeeding.

Table 8. Maternal personality and mood in connection with breastfeeding including women with medical interventions, during labour, birth or postpartum.

Measurement of oxytocin levels

The technique used for measurement of oxytocin levels is of critical importance for the interpretation of data. Radioimmunoassay or RIA is the gold standard for determination of oxytocin levels, whereas some techniques such as Enzyme Linked Immunoassay or ELISA, especially if performed without extraction of the samples, may give rise to 10–100 fold higher values, than those obtained by RIA. This is probably due to the fact that ELISA is a less specific method and may include measurement of fragments or metabolites of oxytocin or even of other material that is not related to the oxytocin molecule. Therefore values obtained with ELISA have to be interpreted with caution [48, 49].

Another important variable for the oxytocin levels obtained is of course the number of and timing of samples collected. As oxytocin is released in short lasting pulses in response to breastfeeding, repeated samples collected at short time intervals are needed in order to demonstrate the pattern of oxytocin release in response to breastfeeding [49]. As oxytocin is released almost immediately after the onset of breastfeeding, samples must be collected soon after initiation of breastfeeding in order to record the release. Also, quick blood sampling is more likely to catch the oxytocin induced peaks than is slow blood sampling because the peaks may become blunted, if blood samples are drawn slowly. A much more detailed discussion of the techniques used for determination of oxytocin levels in plasma as well as on the importance of timing of blood sampling was presented in a previously published paper [1]. Oxytocin levels in saliva have not been convincingly proven to reflect circulating oxytocin levels in connection with breastfeeding and have therefore been excluded from this study [49, 50].


Effects of breastfeeding and other types of breast stimulation on oxytocin levels (3a)

Effects of breastfeeding and skin to skin contact.

Basal levels of oxytocin decreased during the first days postpartum [37]. Maternal oxytocin levels were shown to rise in connection with the onset of suckling or other types of breast stimulation or skin-to-skin contact immediately after birth [1442]. In control studies performed without suckling, or in studies of women formula-feeding their infants, no rise of oxytocin was observed [18, 20, 23].

Pattern of oxytocin release.

A pulsatile pattern of oxytocin release was found in response to breastfeeding in particular during the first days after birth [23, 24, 28, 30, 32, 35, 37, 40]. When samples were collected frequently (every 30th second), up to five short-lasting pulses were observed during the first 10 minutes of breastfeeding [24, 32, 35, 40]. Later on, during lactation, these peaks often coalesced, showing a larger and more protracted rise. Levels generally returned to baseline after 20 minutes of suckling. A second peak of oxytocin was sometimes observed, possibly in connection with suckling of the second breast [37].

Skin-to-skin contact immediately after birth gave rise to an increased oxytocin levels. No short-lasting peaks were observed, rather a more protracted type of oxytocin release [27, 31, 39].

Levels of oxytocin.

Basal levels of oxytocin varied between 0 and 20 pg/mL, when measured with RIA. Oxytocin levels on average rose 5-fold (range 2-10-fold) in response to breastfeeding. The difference in the size of the peaks is due to variation between individual mothers, early versus late breastfeeding, but is also due to methodological reasons. The different assays used for oxytocin determination do not give rise to identical values and furthermore the timing of and, in fact also the duration of blood sampling, influences the size of the peaks. In the experiments in which oxytocin levels were measured with ELISA onset levels were high (132- to 1642 pg/mL) and even then peaks of oxytocin were induced by breastfeeding [14, 51].

In some studies, where mothers and babies were together before start of suckling, peaks of oxytocin were observed even before the onset of suckling, often in response to the baby’s cry [28].

As mentioned above, the magnitude of the rise of oxytocin increased over the duration of lactation [23, 37]. However, the length of suckling during a breastfeed episode did not correlate with oxytocin levels, or with the amount of oxytocin (area under the curve) released in connection with that particular breastfeed. There was, however a strong correlation between the amount of oxytocin released by the individual women at different times in lactation [37].

Oxytocin levels in response to breastfeeding were higher in multiparous than in primiparous women [26].

Basal oxytocin levels were higher in exclusively, compared to partially, breastfeeding women [23, 37], and fell after weaning [37].

Effects of breast massage.

Manual breast massage was associated with a substantial and sustained elevation in oxytocin, without the pulses that occurred during suckling. In fact, more oxytocin was released by breast massage than by suckling [40].

Effects of stress, depression and alcohol.

Women exposed to different types of stress (mental or noise) during breastfeeding had significantly fewer oxytocin peaks in response to breastfeeding than those mothers that were not exposed to a stressor [35]. Mothers with high depression scores had lower oxytocin levels, both before and during breastfeeding [20, 34]. Alcohol consumption (0.4 g/kg, a high amount) prior to breastfeeding also reduced oxytocin release [29].

Oxytocin and breastfeeding outcomes.

In several of the studies, milk yield was measured [22, 26, 2830, 32, 35, 37]. The number of breastfeeding-induced oxytocin pulses during early breastfeeding correlated with milk yield during suckling [30], and with the subsequent duration of lactation [32]. In some studies, a correlation between oxytocin levels and milk yield was found [26, 29]. Higher birth weight of the infant correlated with higher oxytocin levels during suckling at 3–4 months [37].

Effect of mechanical breast stimulation.

Mechanical breast pumping was followed by a rise of oxytocin levels, which was generally of similar amplitude to the release caused by suckling [41]. However, no oxytocin peaks were observed before the onset of mechanical pumping [25]. More oxytocin was released in response to double pumping (both breasts together) compared to single pumping. In addition double pumping produced the highest milk yield, indicating a relationship between oxytocin levels and milk yield [17, 41].

In healthy, late-pregnant (non-breastfeeding) women, mechanical pumping or nipple stimulation caused a release of oxytocin, which was associated with uterine contractions [15, 19]. Oxytocin levels also increased in response to breast stimulation in healthy non-pregnant, non-breastfeeding women [15, 18].

Oxytocin levels with medical interventions (Table 4)

In five of the included articles, data on the effects of medical interventions on oxytocin levels during breastfeeding were reported [24, 30, 32, 39, 42].

Medical interventions in connection with birth influenced the release of oxytocin in response to skin-to-skin contact and suckling. Women who had had a prelabour caesarean section did not release oxytocin in response to skin-to-skin contact and suckling after birth [39]. In women who received a postpartal intravenous infusion of synthetic oxytocin to prevent bleeding in the postpartum period, these effects were significant, i.e. oxytocin was released in response to skin-to-skin contact and suckling [39].

Women, who had had a caesarean section, had significantly fewer pulses of oxytocin during a breastfeeding episode on day two after birth [32], which was correlated with reduced milk yield and reduced subsequent duration of lactation [30].

Women who had received both epidural analgesia and an intravenous infusion of synthetic oxytocin during labour had lower oxytocin levels in connection with breastfeeding two days after birth, compared to those with either intervention alone, or to unexposed women [24]. This decrease was dose-dependent; the more oxytocin and epidural analgesia they had received, the lower their oxytocin levels in response to breastfeeding at two days postpartum [24]. These data must, however, be interpreted with cautions as they were measured with ELISA, which is a less specific method, and which gives rise to higher basal levels of oxytocin than RIA.

Prolactin levels (Table 5)

Effect of breastfeeding.

Prolactin levels were measured in 16 of 29 studies [14, 15, 20, 21, 2325, 2830, 32, 35, 37, 38, 40, 41]. Prolactin levels rose in response to breastfeeding in all 15 studies. The rise of prolactin in response was gradual and generally not significant until 10 minutes after suckling commencement. Sustained prolactin elevations persisted for at least 60 minutes [21, 2325, 28, 32, 35, 37, 38, 40].

No rise of prolactin was observed in the absence of breastfeeding or prior to breastfeeding [24, 28]. Nor did prolactin levels increase with nipple stimulation or breast tactile massage [15, 40]. Stress (mental and noise) during breastfeeding did not influence breastfeeding-induced prolactin release levels [33].

There was a significant positive correlation between the duration of the breastfeeding episode and median prolactin levels during these episodes [24, 30]. In addition there was a clear correlation between basal oxytocin and prolactin levels [30], and between oxytocin variability (reflecting pulsatility) and the rise in prolactin in response to suckling [24]. Prolactin levels, both basal and in response to suckling decreased over the course of lactation [23, 37].

Prolactin and breastfeeding outcomes.

No correlation was found between suckling-induced prolactin levels and the amount of milk produced during a particular breastfeeding session [28, 35, 37]. In one study, prolactin levels at 3–4 months predicted the remaining duration of exclusive breastfeeding [37]. This could reflect the amount and intensity of suckling performed by the breastfeeding infant during previous breastfeeding sessions.

Effect of mechanical breast stimulation.

Prolactin levels increased in response to mechanical breast pumping, reaching similar levels as during breastfeeding [17, 25, 41]. When two pumps were applied at the same time, prolactin levels were higher compared to single pumping [17, 41] and milk yield was greater [41].

Effect of medical interventions.

The rise of prolactin levels seen in response to breastfeeding at two days postpartum was significantly lower in women who had had an emergency [24] caesarean section, compared to women who had had a vaginal birth [32].

Breastfeeding at two days postpartum did not induce any significant rise of prolactin levels in women who had received epidural analgesia during labour [24]. In contrast, women who had received an intravenous infusion of synthetic oxytocin during labour, had significantly higher suckling-induced prolactin levels, compared to women, who had not had any interventions. [24].

Stress variables (Table 6)

Stress variables were measured in nine of the included articles/references [14, 1618, 20, 30, 32, 34, 42]. The data are reported in 12 articles, since for one of the included studies [24] breastfeeding induced effects on stress levels were reported in 3 additional separate articles [4345].

Effect of breastfeeding.

Suckling was seen to induce a significant decrease in ACTH (adrenocorticotrophic hormone, which stimulates the release of cortisol from the adrenal cortex) [18, 43]. Suckling also induced a significant decrease in cortisol levels [1618, 20, 32, 43].

The decrease in ACTH levels was positively correlated to the duration of suckling during a breastfeeding episode [43] and negatively correlated to both oxytocin levels and oxytocin variability [18, 43].

The decrease in cortisol levels during suckling was significantly correlated with the decrease of ACTH levels, but not with the duration of the breastfeeding episode. In contrast, the decrease in cortisol levels was significantly correlated to the duration of skin-to-skin contact prior to breastfeeding [43].

No decrease of ACTH or cortisol levels was observed in control experiments without breastfeeding, or after formula-feeding [16, 18].

Both systolic and diastolic blood pressure decreased in response to breastfeeding [32, 44, 45]. Basal blood pressure was significantly reduced in women after 6 weeks of breastfeeding, but each breastfeeding session was still associated with a further decrease in blood pressure [45].

Breastfeeding induced a long term stress buffering effect; after an episode of breastfeeding, physiological responses to external stressors, including heart rate and cortisol response, were reduced [20].

Effect of mechanical breast stimulation.

Mechanical breast-pumping in lactating women decreased ACTH and cortisol levels. The nadir of ACTH levels coincided with the maximal rise in oxytocin levels [18]. A decrease of ACTH and cortisol levels was also observed in response to mechanical pumping in non-lactating normal cycling women, demonstrating that this effect is not restricted to lactating women. Again, the lowest levels of ACTH correlated in time with the highest oxytocin levels [18].

Effects of medical interventions.

No significant differences were found in the decrease of cortisol levels during breastfeeding between vaginal delivery or emergency caesarean section [32].

Both basal and breastfeeding-related cortisol levels were lower in women who had experienced epidural labour analgesia compared to women who had received an intrapartum intravenous infusion of synthetic oxytocin, either alone or in combination with epidural analgesia [43].

Emergency caesarean section did not influence the fall in blood pressure caused by breastfeeding two days after birth [32]. In contrast, basal blood pressure in women who had received epidural analgesia during birth was significantly lower than in the other groups, and no decrease in blood pressure was observed in response to breastfeeding [44].

Mothers who had a prelabour caesarean section with immediate skin-to-skin contact and who had also received a postpartum intravenous infusion of synthetic oxytocin, showed a significant increase in antioxidant measures. The postpartum rise in oxytocin levels was correlated with these effects [42].

Digestion, metabolism and nutrition (Table 7)

Measures of digestion, metabolism and nutrition were reported in three of the included articles [22, 46, 47]. The data are reported in five articles, since for one of the included studies [37] breastfeeding induced effects on somatostatin release were reported in two separate articles [46, 47].

Effect of breastfeeding.

Somatostatin inhibits the activity of the endocrine system of the gastrointestinal tract, e.g the release of gastrin from the stomach, that stimulates gastric acid secretion and insulin and glucagon from the pancreas that, participate in the control of glucose levels [46, 47]. Levels of somatostatin tended to fall in response to suckling at 3–4 days after birth. In contrast, somatostatin levels consistently rose in response to suckling at 3–4 months postpartum [46]. Average somatostatin levels recorded in connection with breastfeeding at 3–4 months postpartum were significantly higher than those obtained at 3–4 days after birth [46]. A strong correlation was found between the release of somatostatin at 3–4 days and at 3–4 months postpartum [46].

The weight of the newborn correlated strongly and inversely with somatostatin levels obtained at 3–4 days and 3–4 months postpartum and also with placental weight [46].

Somatostatin levels were higher at 4 days in smoking compared to non-smoking women. Smoking was associated with shorter duration of breastfeeding [47].

Breastfeeding was also associated with a rise of the levels of thyroid stimulating hormone (TSH) [20].

Thirst was increased in response to breastfeeding, and the amount of water ingested after breastfeeding was associated with intensity of thirst reported [22].

Personality traits and mood (Table 8)

Personality traits and mood were reported in five of the included articles [20, 30, 34, 36, 39, 51].

The data are reported in six articles, since for one of the included studies [24] data on personality traits are presented in a separate article [51].

Effect of breastfeeding.

In four studies [30, 36, 39, 51] the scores of items related to anxiety, tension and aggression were decreased, and the scores of items related to social functioning were elevated, in comparison to a normative group of non-pregnant non-breastfeeding women of the same age. These changes persisted for 6 months, if the mothers continued to exclusively breastfeed [51].

In these studies, oxytocin levels during breastfeeding correlated negatively with KSP anxiety and tension scores, and positively with scores in social functioning [30, 36]. In other studies, women with higher scores on depression “Edinburgh Postnatal Depression Scale” (EPDS) and anxiety “State-Trait Anxiety Inventory” (STAI) had lower oxytocin release during breastfeeding at 8 weeks [20, 34].

Effect of medical interventions.

Mothers who had given birth by pre-labour or emergency caesarean section did not have the full pattern of KSP maternal adaptations compared to women who had a vaginal birth [30, 39]. In women who received epidural analgesia (without infusion of synthetic oxytocin), no maternal adaptations on KSP were found at eight weeks. However, adaptations were restored at two-six months in women who exclusively breastfed [24].

Infusions of synthetic oxytocin during labour slightly enhanced the KSP adaptations observed in connection with breastfeeding two days postpartum. The dosage of synthetic oxytocin administered was negatively correlated with the scores of inhibition of aggression [39, 51]. In addition, infusions of synthetic oxytocin restored the lack of the changes in the KSP seen in mothers who had received epidural analgesia alone. Finally, oxytocin infusions given postpartum restored the lack of changes in the KSP pattern found in women who had had a prelabour caesarean section [51].


Articles in which blood/plasma levels of oxytocin levels in response to breastfeeding had been recorded were included in this systematic literature review. In some of the selected articles (and in some of the associated articles based on the same clinical studies) information was also reported regarding prolactin, stress, nutrition, metabolism, as well as personality and mood. Some studies also reported on effects of medical interventions in connection with birth. Since the primary objective of this systematic literature review was to summarize studies that measured breastfeeding-related oxytocin levels, we obviously did not identify all studies regarding the secondary variables mentioned above. We chose to include the secondary oxytocin linked effects because data obtained within the same article may reveal more causal with oxytocin relationships than data obtained from different articles. In a sense this gives a deeper meaning to the oxytocin levels recorded because they also become a proxy for the secondary oxytocin linked endocrine, physiological and psychological effects.

The studies included in this review differ in many ways. Some studies were performed during early breastfeeding and some later on during established breastfeeding. In some studies oxytocin release in response to suckling was studied immediately after birth. The timing of blood samples was also very different. The number of blood samples collected in the different studies varied between 2 and 24 and the frequency by which samples were collected between 30 seconds and 20 minutes. In other studies, as much as 24 blood samples were collected, some of them with 30 second intervals. We have tried to describe and summarize the results regarding breastfeeding induced effects on oxytocin level despite these methodological differences.

All of the studies showed that suckling is associated with oxytocin release [1442]. In early breastfeeding, the release of oxytocin occurred in pulses, which later on during breastfeeding coalesced into a large sustained peak [23, 24, 28, 30, 32, 35, 37, 40]. There were correlations between oxytocin levels and milk yield in several of the included studies and also correlations with duration of breastfeeding and weight of the newborn [14, 22, 25, 26, 2830, 32, 3537, 52]. The correlation between milk yield and the number of oxytocin pulses is of particular interest as each peak of oxytocin is linked to milk ejection [53]. Taken together, these data suggest that oxytocin levels in response to breast feeding correlates with milk production and also for the duration of breastfeeding.

From this perspective, it is of interest that the number of oxytocin peaks released during breastfeeding was reduced by relatively minor stressors, such as noise and mental activity (concentration), indicating that both an “external” and “internal” environment characterized by calmness are of importance for the breastfeeding mother in order promote the milk-ejection reflex and thereby milk production [35]. Also maternal mood influenced oxytocin levels, as depression was associated with lower oxytocin release in response to breastfeeding [34].

Oxytocin produced in the supraoptic (SON) and paraventricular nuclei (PVN) of the hypothalamus is released from the posterior pituitary into the circulation in response to breastfeeding in order to stimulate milk ejection. However, oxytocin is at the same time released from oxytocinergic nerves that project from the PVN in the hypothalamus to many regulatory areas in the brain. In this way breastfeeding may be accompanied by many different physiological and psychological adaptations, which help mothers to cope with the demands of breastfeeding and motherhood. For a more elaborate description of the central effects of oxytocin and how oxytocin release into the circulation and the brain are coordinated, see Uvnäs Moberg et. al. 2019 [1].

The present results identified multiple adaptive effects of breastfeeding, which are linked to activation of the oxytocin system. These effects include facilitation of prolactin release, decreased activity in the stress system (HPA axis: ACTH and cortisol and the sympathetic nervous system: blood pressure and heart rate) and increased function of the parasympathetic part of the autonomic nervous system, including activation of the gastrointestinal tract by the vagal nerve. Oxytocin release was also correlated with mental adaptations that facilitate motherhood such as increased levels of social interaction and decreased anxiety [1]. The oxytocinergic pathways in the brain that may be involved in these actions are illustrated in Fig 2.

Fig 2. Schematic illustration of the oxytocin pathways activated during breastfeeding.

As reported in this review prolactin was released by suckling [15, 20, 21, 2325, 2830, 32, 35, 37, 38, 40, 41]. The release of prolactin, however, differed from oxytocin release caused by suckling in many ways. During a breastfeeding episode, oxytocin is released within minutes and in a pulsatile pattern, whereas prolactin levels increase more slowly, are not pulsatile and more long lasting than oxytocin levels. Nor could prolactin be released by breast-massage and prolactin release was less sensitive to acute stressors than oxytocin release. Altogether, these data indicate that oxytocin and prolactin release are in part mediated by separate mechanisms and that prolactin release is more resilient to stress than is oxytocin [35]. Nevertheless, oxytocin exerts an over-riding facilitating influence on prolactin release, via the oxytocin-containing nerves that extend from the PVN to the prolactin-producing cells in the anterior pituitary, as shown above and in Fig 2. In this way, oxytocin stimulates milk production as well as milk ejection [7].

Breastfeeding was also linked to anti-stress effects, with decreases in ACTH, cortisol and blood pressure during a breastfeeding episode [1618, 20, 30, 32, 34, 4244, 51]. The decrease in ACTH levels was linked to the duration of suckling reflecting the inhibitory effect of oxytocin released within the PVN on Corticotropin-releasing factor (CRF), which regulates ACTH release. In addition, oxytocin may have a direct inhibitory effect on ACTH via nerves linking the PVN to ACTH-producing cells in the anterior pituitary (Fig 2).

In these studies, the decrease in cortisol levels was not only associated with reduced ACTH levels, but also to the duration of skin-to-skin contact. This latter effect most likely reflects the influence of oxytocin nerves that project to areas in the brain that regulate the function of the autonomic nervous system, and which are activated in response to stimulation of sensory nerves that originate in the skin. In the same way the decrease in blood pressure induced by suckling involves an oxytocin mediated decrease of the activity in the sympathetic nervous system [7, 49].

Lowering of ACTH and cortisol levels was also induced in response to suckling in non-breastfeeding women, suggesting that suckling may induce stress reduction in the absence of breastfeeding [18].

One of the studies showed that the sensitivity to stress is decreased for some hours following breastfeeding, indicating that breastfeeding is, in addition to the acute anti stress effect, linked to a more long-term stress-buffering effect [20]. Similar findings have been reported in other studies [54]. Taken together these data show that mothers are subject to powerful anti-stress effects during suckling, which may help them relax and adapt to breastfeeding. The antioxidant effects observed in response to skin-to-skin contact represent yet another “health” promoting effect of breastfeeding [44].

The data in this review also showed that thirst is increased and that, absorption, digestion and metabolism of nutrients were optimized by breastfeeding-related oxytocin release [22, 46, 47]. These effects assist in adapting the mother to the high demands of nutrition and fluid intake during breastfeeding. Oxytocin influences the gastrointestinal function by increasing the function of the vagal nerve. For example, it may decrease the release somatostatin, a hormone produced in the gastrointestinal tract, which exerts inhibitory effects on gastrointestinal function [7]. Oxytocin (and breastfeeding) lower the levels of somatostatin and thereby the function of the gastrointestinal tract increases. The finding of an inverse relationship between levels of oxytocin and somatostatin (which inhibits the activity of the endocrine system of the gastrointestinal tract) also supports the important role oxytocin has in adapting maternal metabolism to the demands of breastfeeding [55].

In several of the included studies, the personality inventory, Karolinska Scales of Personality (KSP) was applied to breastfeeding women [30, 36, 39, 51]. The KSP has 135 items in a four-point response format. It consists of 15 self-reported scales that can be divided into the anxiety proneness scales, the extroversion related scales, the socialization scale, the social desirability scale and the aggression-hostility related scales. The KSP is standardized for sex and age and the normative sample is based on 200 non-pregnant or lactating randomly selected women. The test has a high validity and retest stability [56, 57].

It seems that oxytocin release during breastfeeding influences maternal psychology in a way that facilitates motherhood, i.e. levels of anxiety and aggression are decreased, and social functioning increased. Breastfeeding is also linked to increased sensitivity in the mother and other mental adaptations that facilitate motherhood [6]. In a study performed by Strathearn et. al., 2009, dopamine activity in the maternal brain was increased in response to their own smiling baby’s photo. This increase in dopamine activity was related to maternal oxytocin levels and also to secure attachment [58].

These changes, which are linked to oxytocin levels are beneficial for the mother and might constitute a slight reflection of the more developed maternal behaviours that occur in other mammals in response to central oxytocin release, during parturition and suckling [59]. Altogether, these data indicate that the oxytocin release associated with breastfeeding contributes to the development of positive maternal experiences, psychological skills and physiological adaptations.

Mechanical breast stimulation was associated with oxytocin release and milk yield of similar magnitude as breastfeeding. Mechanical breast stimulation also stimulated prolactin release and decreased ACTH and cortisol levels [17, 25, 41]. These data suggest that mechanical breast stimulation is associated with at least some of the oxytocin-induced adaptations seen in response to breastfeeding [7].

By contrast, none of these adaptations were induced when mothers were bottle-feeding their babies [23]. Also, other studies have demonstrated that bottle feeding mothers are less sensitive in the communication with their babies and that they have higher stress levels than breastfeeding mothers [60].

Medical interventions during labour and birth have been associated with disturbed breastfeeding [810]. Such effects might potentially be mediated by a disrupted function of the oxytocin system during labour and birth, which then persists during breastfeeding. During labour the release of oxytocin is triggered by pressure from the baby’s head on the cervix and the vagina in response to uterine contractions (the Ferguson reflex). Less oxytocin may be released during caesarean sections (especially by prelabour caesarean sections due to the absence of uterine contractions and labour) than during vaginal birth [1]. Also, epidural analgesia may reduce oxytocin release in connection with birth. Epidural analgesia blocks the transmission in pain fibres, but also the transmission in the nerve fibres mediating the Ferguson reflex and thereby oxytocin release [61]. It is biologically plausible that such effects on oxytocin release induced by medical interventions in connection with birth could become long-lasting, with consequences for breastfeeding outcomes and other oxytocin-linked effects [1, 62]. In fact, results from some of the included studies indicate that oxytocin release in response to breastfeeding may be deranged by medical interventions. In women who had an emergency cesarean section, oxytocin release in response to early breastfeeding was significantly reduced [30, 32]. Prolactin release and maternal adaptations were also reduced, possibly as a consequence of the reduced release of oxytocin in the brain during breastfeeding [30, 32]. In another study mothers who had had a prelabour caesarean section, skin-to-skin contact immediately after birth did not stimulate maternal oxytocin release or maternal adaptations as measured with the KSP 2 days later [39]. Epidural analgesia was linked to decreased prolactin levels and decreased maternal adaptations [24, 51]. Taken together these data suggest that mothers who have given birth by caesarean or have had an epidural analgesia might at least initially during breastfeeding have a compromised function of the oxytocin system. This may contribute to the breastfeeding difficulties that women can experience following caesarean section and epidural analgesia [8, 9].

Both prolactin release, and maternal mental adaptations were, however, more strongly developed in women who received synthetic oxytocin infusions during labour, in comparison to those who did not receive any medical interventions. Some of these effects were dose-dependent [24, 51]. In addition, infusions of synthetic oxytocin seemed to counteract the negative influence of epidural analgesia on maternal mental adaptations [51]. Infusions of synthetic oxytocin also restored the release of endogenous oxytocin in response to early skin-to-skin contact, as well as the development of maternal mental adaptations, which was otherwise not developed following prelabour caesarean section [39]. These central effects of synthetic oxytocin are probably induced indirectly via nervous reflexes, such as reinforcement of the Ferguson reflex (1).

In addition, the repetitive release of endogenous oxytocin during breastfeeding may counteract some of the negative effects of caesarean section and epidural analgesia on prolactin release and maternal mental adaptations [51]. In this way breastfeeding may counteract some of the negative consequences caused by medical interventions that are linked to reduction of oxytocin release in labour and birth.

The repetitive release of oxytocin occurring during breastfeeding may also explain why basal blood pressure was significantly decreased after 6 weeks of breastfeeding [45]. Such long term effects of repeated oxytocin exposure may also explain why breastfeeding is linked to long term health-promoting effects [63]. In particular, there are long term benefits for different types of cardiovascular disease, including hypertension, stroke, heart infarction and diabetes type 2, with greater effects for longer duration of lactation [4, 5, 6466].

Also the infants profit from breastfeeding beyond being exposed to the beneficial components of breastmilk [67]. Skin-to-skin contact and suckling promote oxytocin release also in the baby [7], with possible long term beneficial effects on bonding, health and wellbeing [4, 6, 7].

Taken together this review shows that breastfeeding is more than a transfer of breastmilk from mother to infant. By stimulating the release of oxytocin into the circulation and into the brain, it not only stimulates milk ejection, it also stimulates neuroendocrine processes that facilitate milk production and maternal physiological and psychological adaptations. Also, the baby is exposed to oxytocin during breastfeeding with possible long-term beneficial effects on health and wellbeing. When considering these positive aspects of breastfeeding it is obvious that breastfeeding should be valued beyond its role as a source of breastmilk for the baby.

Conclusions and implications for clinical practice

The Baby-Friendly Hospital Initiative (BFHI) which is supported by the WHO focuses on breastfeeding for healthy, mother-infant dyads. To stimulate and facilitate breastfeeding “The ten steps to successful breastfeeding” have been formulated. They recommend immediate and uninterrupted skin-to-skin contact and support of mothers to initiate breastfeeding as soon as possible after birth. They also support mothers to initiate and maintain breastfeeding and how to manage common breastfeeding difficulties. Further, they support the mothers and their infants to remain together and to practise rooming in the maternity ward and not to provide breastfed newborns any food or fluids other than breast milk, unless medically indicated. Finally, they also provide support for mothers to recognize and respond to their infants’ cues for feeding. There is substantial evidence that implementing the “Ten Steps” significantly improves breastfeeding rates. A systematic review of 58 studies on maternity and newborn care published in 2016 demonstrated clearly that adherence to the Ten Steps impacts early initiation of breastfeeding immediately after birth, exclusive breastfeeding, and total duration of breastfeeding [68].

In fact, all the practical recommendations described within “The ten steps” are consistent with an optimized stimulation of oxytocin release. As summarized in this review oxytocin is released in response to skin-to-skin contact and breastfeeding to cause milk ejection and to promote milk production. It also induces physiological changes and psychological adaptations to facilitate motherhood. The relevance of the “Ten steps” recommendations is therefore supported by their link to activation of the oxytocin system. More research should be performed to increase the knowledge about the link between the practical recommendations mention above in BFHI and the activation of the oxytocin system in mothers and their babies.

Repeated exposure to oxytocin with each episode of breastfeeding, may contribute to the lifelong benefits of breastfeeding for mother and baby and it may even counteract some negative consequences of medical interventions.

It is of importance that health professionals receive information about how oxytocin and oxytocin linked effects promote breastfeeding and how this system is reinforced by the recommendations of BFHI.


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