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Traditional milk transformation schemes in Côte d’Ivoire and their impact on the prevalence of Streptococcus bovis complex bacteria in dairy products

  • Aimé R. Sanhoun,

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Validation, Visualization, Writing – original draft, Writing – review & editing

    Affiliations UFR des Sciences et Technologies des Aliments (STA), Université Nangui Abrogoua, Abidjan, Côte d’Ivoire, Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire

  • Sylvain G. Traoré,

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Writing – review & editing

    Affiliations Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire, Département Biochimie-Génétique, Université Péléforo Gon Coulibaly, Korhogo, Côte d’Ivoire

  • Kossia D. T. Gboko,

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

    Affiliations Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire, École Inter-États des Sciences et Médecine Vétérinaires de Dakar, Dakar, Sénégal

  • Jérôme Kirioua,

    Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

    Affiliation Département Biochimie-Génétique, Université Péléforo Gon Coulibaly, Korhogo, Côte d’Ivoire

  • Fabienne Kurt,

    Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

    Affiliation Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

  • Nize Otaru,

    Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

    Affiliation Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

  • Patriz Iten,

    Roles Data curation, Formal analysis, Investigation, Methodology, Writing – review & editing

    Affiliation Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

  • Dasel W. M. Kaindi,

    Roles Conceptualization, Formal analysis, Methodology, Writing – review & editing

    Affiliation Department of Food Science, Nutrition and Technology, University of Nairobi, Nairobi, Kenya

  • Bernd Kreikemeyer,

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – review & editing

    Affiliation Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Medical Centre Rostock, Rostock, Germany

  • Pierre Renault,

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Writing – review & editing

    Affiliation Institute Nationale de la Recherche Agronomique, UMR 1319 MICALIS, Jouy-en-Josas, France

  • Daouda Dao,

    Roles Conceptualization, Investigation, Methodology, Writing – review & editing

    Affiliations Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire, UFR Sciences Économiques et Gestion (SEG), Université Félix Houphouët Boigny, Abidjan, Côte d’Ivoire

  • Jan Hattendorf,

    Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

    Affiliations Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, University of Basel, Basel, Switzerland

  • Leo Meile,

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – review & editing

    Affiliation Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

  • Marina Koussemon,

    Roles Conceptualization, Formal analysis, Investigation, Methodology, Supervision, Writing – review & editing

    Affiliation UFR des Sciences et Technologies des Aliments (STA), Université Nangui Abrogoua, Abidjan, Côte d’Ivoire

  • Christoph Jans ,

    Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

    christoph.jans@hest.ethz.ch

    Affiliation Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

  •  [ ... ],
  • Bassirou Bonfoh

    Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

    Affiliations Centre Suisse de Recherches Scientifiques en Côte d’Ivoire (CSRS), Abidjan, Côte d’Ivoire, Department of Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland, University of Basel, Basel, Switzerland

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Traditional milk transformation schemes in Côte d’Ivoire and their impact on the prevalence of Streptococcus bovis complex bacteria in dairy products

  • Aimé R. Sanhoun, 
  • Sylvain G. Traoré, 
  • Kossia D. T. Gboko, 
  • Jérôme Kirioua, 
  • Fabienne Kurt, 
  • Nize Otaru, 
  • Patriz Iten, 
  • Dasel W. M. Kaindi, 
  • Bernd Kreikemeyer, 
  • Pierre Renault
PLOS
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Abstract

The Streptococcus bovis/Streptococcus equinus complex (SBSEC) and possibly Streptococcus infantarius subsp. infantarius (Sii) are associated with human and animal diseases. Sii predominate in spontaneously fermented milk products with unknown public health effects. Sii/SBSEC prevalence data from West Africa in correlation with milk transformation practices are limited. Northern Côte d’Ivoire served as study area due to its importance in milk production and consumption and to link a wider Sudano-Sahelian pastoral zone of cross-border trade. We aimed to describe the cow milk value chain and determine Sii/SBSEC prevalence with a cross-sectional study. Dairy production practices were described as non-compliant with basic hygiene standards. The system is influenced by secular sociocultural practices and environmental conditions affecting product properties. Phenotypic and molecular analyses identified SBSEC in 27/43 (62.8%) fermented and 26/67 (38.8%) unfermented milk samples. Stratified by collection stage, fermented milk at producer and vendor levels featured highest SBSEC prevalence of 71.4% and 63.6%, respectively. Sii with 62.8% and 38.8% as well as Streptococcus gallolyticus subsp. macedonicus with 7.0% and 7.5% were the predominant SBSEC species identified among fermented and unfermented milk samples, respectively. The population structure of Sii/SBSEC isolates seems to reflect evolving novel dairy-adapted, non-adapted and potentially pathogenic lineages. Northern Côte d’Ivoire was confirmed as area with high Sii presence in dairy products. The observed production practices and the high diversity of Sii/SBSEC supports in-depth investigations on Sii ecology niche, product safety and related technology in the dairy value chain potentially affecting large population groups across sub-Saharan Africa.

Introduction

Cow milk plays an important role in pastoralists and urban consumers’ diets across the African continent [1]. For pastoralists, milk and livestock products contribute up to 85% and 63% to their diets and household incomes, respectively [2]. However, homemade processing renders milk particularly perishable and conducive to the spread of pathogenic microorganisms and their toxic metabolites [35]. Milk is traditionally fermented to protect it from spoilage, to inhibit outgrowth of pathogenic microorganisms, to prolong shelf life, and to yield popular and regionally-specific fermented dairy products (FDP) in many African countries [1, 6].

During the past decade, Streptococcus infantarius subsp. infantarius (Sii) and Streptococcus gallolyticus subsp. macedonicus (Sgm), both members of the Streptococcus bovis/Streptococcus equinus complex (SBSEC), were identified as the predominant lactic acid bacteria (LAB) at 108 CFU mL-1 in traditional African FDP, particularly in East African products such as ‘gariss’ in Sudan (camel milk) or ‘suusac’ in Kenya and Somalia (camel milk) but also in ‘fènè’ in Mali (cow milk), and sour milk in Abidjan (southern Côte d’Ivoire, cow milk) [1, 710]. SBSEC members and possibly some Sii lineages are associated with various human and animal diseases including colorectal cancer, infective endocarditis, bacteraemia and potentially haemorrhoids [1114]. Unlike human Sii, African Sii dairy variants present an adapted lactose metabolism similar to that of Streptococcus thermophilus based on lacS and lacZ. Multiple African Sii strains are capable of inhibiting certain foodborne pathogens such as Listeria spp. However, no explicit virulence factors are described neither for human or food-derived Sii [8, 1517].

Population structure analysis of Sii suggests a separation of several dairy lineages from a main presumed pathogenic lineage, but also a shared clade of East African human (EAH) and West African dairy (WAD) strains [14, 18]. Coupled with the predominance in many FDP, these features suggest a pivotal role of Sii in African FDP [1]. However, the close relationship of certain West African dairy Sii with East African human strains and for some strains also with human blood isolates requires enhanced knowledge of Sii prevalence, population structure and potential reservoirs particularly in West Africa [18]. The identification of close contact with livestock and livestock primary products as well as rural habitats as indicators for faecal carriage of Sii in humans [19] raises interest in the prevalence of Sii in the traditional milk transformation schemes and the production processes associated with them.

Previously, southern Côte d’Ivoire featured a Sii prevalence in FDP ranging from 32% to 40% [9]. However, Sii prevalence in the north of the country was not assessed, although northern Côte d’Ivoire represents the major cow milk production area with 70% of the national cattle population and the Korhogo region alone is responsible for nearly 50% of its dairy production [2022]. Northern Côte d’Ivoire with its main city Korhogo is furthermore the first centre of settlement of a ~40,000-people-sized Fulani transhumant population that also inhabit large parts of sub-Saharan West Africa including Mali and Burkina Faso for cross-border trading [23, 24]. Northern Côte d’Ivoire could therefore provide relevant data on cow milk processing and Sii/SBSEC prevalence for this large pastoral area of West Africa. Furthermore, the link to production practices is unknown, which is a key aspect regarding the socio-economic impact of milk and FDP for the livelihoods of these populations. Korhogo with approximately 245,000 agro-pastoralists out of the total population of 537,000 [25, 26] provides an ideal study area to assess the prevalence and introduction of Sii into the dairy chain. In addition, Korhogo may serve as a representative example for the implementation of a research-to-action study involving dairy stakeholders and policies for sustainable livelihoods for these populations. Therefore, this study aimed to assess the prevalence of Sii and other SBSEC bacteria in connection with production and processing practices along the informal cow milk chain in Korhogo.

Materials and methods

Growth media and culture conditions of bacteria

Cultivation of lactic acid bacteria and presumptive SBSEC were performed using M17 agar and broth medium (Biolife, Milan, Italy) at 30°C under aerobic conditions and NaCl/peptone diluent (Sigma Aldrich, Buchs, Switzerland) [9]. All media and solutions were prepared using distilled H2O and sterilized by autoclaving at 121°C for 15 min.

Reference strains

Sii CCUG43820T, Sii CJ18, S. thermophilus DSM 20259, Enterococcus faecalis DSM 20478T and Enterococcus faecium DSM 20477T were used as reference strains. All strains were obtained from the Culture Collection of the University of Gothenburg (CCUG, Gothenburg, Sweden), Deutsche Stammsammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany) and the culture collection of the Laboratory of Food Biotechnology (ETH Zürich, Zürich, Switzerland).

Sampling of milk products along the supply chain

From January to May 2014, the purpose of the project was shared with all stakeholders prior to a cross-sectional study carried out from May to August 2014. In collaboration with Fulani chiefs and the national laboratory for agricultural developmental support (Laboratoire National d'Appui au Développement Agricole, LANADA), 30 farms representing the starting points of milk supply chains were identified within a radius of 12 km around Korhogo. An additional 30 unfermented milk collectors and 13 artisanal milk product vendors were randomly selected along the milk chains and systematically considered for inclusion. Collectors were not necessary linked to farms or to vendors and vice versa. The inclusion criteria for all the actors were: age above 18 years, agreement to answer the questionnaires and willingness to deliver milk samples.

Milk samples were distinguished into FDP and unfermented milk according to manufacturers’ information and pH measurements. Milk samples were collected into sterile 15-mL Falcon tubes (Bioswisstec, Schaffhausen, Switzerland) at each stage of the supply chain according to the availability of the milk types. Milk samples were stored on ice from field to the laboratory and kept at -20°C prior to analysis [8, 9]. A total of 120 cow milk samples comprised of 48 spontaneously fermented and 72 unfermented milk samples were collected. Milk sample aliquots were taken aseptically to determine the pH values using pH strips (MColorpHastTM pH 4.0/7.0, SigmaAldrich, Buchs, Switzerland) and the temperature using a digital thermometer (DT150, Summit, Inchon, Korea), the environment temperature at the time of sampling and GPS coordinates at each collection site were recorded.

Survey on milk production practices along the supply chain

Individual questionnaire-based interviews related to milking practices, milk storage, utensils washing, and processing practices were carried out with study participants to collect quantitative data on milk production practices. Observations and group discussions helped to obtain qualitative data. A final restitution workshop involving all stakeholders was organised to define sustainable interventions for the development of dairy value chains in the greater Korhogo region.

Microbiological analysis of milk samples for SBSEC members

Enumeration of presumptive coccoid lactic acid bacteria.

Enumeration of coccoid LAB was performed using M17 agar media (Biolife, Milan, Italy) [9] and specific rules for preparation of milk and milk products standard [27]. In short: milk samples were serially diluted and 0.1 mL of dilutions 10−6 to 10−8 for FDP and 10−1 to 10−3 for unfermented milk were plated onto M17 agar (Biolife). Plates were incubated aerobically at 30°C for 24 h. Colonies of interest were white or milky, large slimy or pinpoint (0.5 to 1 mm diameter). Plates with 10 to 300 visible colony forming units (CFU) were selected for enumeration.

Characterization of presumptive SBSEC bacteria.

Isolation and purification of bacteria from M17 agar was performed until isolates were considered as pure by visual streak observation [8]. Gram classification and catalase activity of isolates was determined using 3% KOH and 3% H2O2, respectively [8].

Identification of purified bacteria isolates by molecular tools

DNA was extracted from catalase-negative and Gram-positive bacteria single colonies using a simple cell-lysis [28]. General PCR conditions involved 2X PCR master mix (ThermoFisher, Zug, Switzerland), 1 μM final primer concentration and nuclease-free sterile H2O to a final volume of 25 μL per reaction. Primers were obtained from Microsynth AG (Balgach, Switzerland).

Molecular identification involved multiple PCR assays. Bacteria DNA was subjected to a multiplex SBSEC-specific PCR assay [29]. Subsequently, isolates were processed using rep-fingerprinting by GTG5 primer [9, 30] for clustering of presumptive duplicate isolates per sample. One isolate per fingerprint type per sample was subjected to groEL amplification and sequencing for subspecies identification [14].

Based on rep-fingerprints and sample origin, 24 isolates (23 Sii and 1 Sgm) were selected to provide maximum diversity to investigate the population structure via a SBSEC-specific multi-locus sequence typing (MLST) scheme and the presence of lacS/lacZ genetic adaptations. The MLST scheme was based on the amplification and sequencing of sections of the 10 genes ddl, gki, glnA, mutS, mutS2, pheS, proS, pyrE, thrS, tpi [14]. DNA sequence curation and analysis for all applications, MLST analysis and clonal complex definition (7 out of 10 alleles shared with at least one other member) were performed as previously described [14]. DNA-sequence-based phylogenetic trees were constructed by the Maximum likelihood algorithm and 200 bootstrap replications in MEGA 7.0 [31]. MLST sequence and profile data was deposited on www.pubmlst.org.

Statistical analysis

Data from survey and laboratory analyses were compiled in Microsoft Access 2013 (Microsoft Corporation, Redmond, WA, USA). Descriptive statistics were done using a t-test with 95% confidences intervals (CI) and one factor analysis of variance (ANOVA) allowed to determine the significant influence of different production factors (p < 0.05) in SPSS Statistics 20.0 (IBM Corp., Armonk, NY, USA).

Ethical approval

Ethical approval: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the national ethics committee in Côte d'Ivoire (N° 018/MSLS/CNER-dkn) and in Switzerland by the Ethics Committees of ETH Zurich (EK 2013-N-78) and Kantonale Ethik Kommission Zurich (KEK-StV-Nr. 47/14).

Informed consent: Informed consent was obtained from all individual participants included in the study.

Results

Milk supply chain organization and processes

Milk production system.

From the 30 farms visited, 837 cows were counted. Predominant breeds were 48% crossbreeds [Bos indicus x Bos taurus]. The daily farm production average was 24.8 L (standard deviation SD 21.1). Out of these, 0.93 L (SD 0.57) (3.6%) were kept for home consumption. The rest was sold through the milk collectors who collect the milk from farm to farm over 40 kilometers or directly in Korhogo town. The produced and collected milk quantities per month were estimated at 744 L and 1959 L for farmers and collectors, respectively. Vendors sold about 1266 L per month. Producers and collectors sold raw cow milk only and vendors sold both fermented and unfermented milk. Metal bowls (16%), calabashes (7%) or plastic containers (77%) were utensils used during hand milking (Fig 1).

Socioeconomics of the milk supply chain.

The exploratory site visits allowed identification of (i) raw milk producers (n = 30; 73% Fulani; 96.7% men and 3.3% women), (ii) raw milk collectors (n = 30; all Fulani men) and (iii) FDP and unfermented milk vendors (n = 13; 58.3% Fulani; 7.7% men and 92.3% women) as the key groups of the dairy supply chain in Korhogo. Tasks around the milk activities were clearly distributed. The milk production in peri-urban areas and its transportation to town were done by men only while dairy products preparation and selling in retail outlets are mostly women affair indeed. Milk production was a secondary income activity after crop for the producers. In contrast, milk trading was the key activity of collectors who usually gathered in teams of two or three persons.

Two remuneration systems exist between collectors and producers: (i) private collection system with direct purchase with immediate payment (18%) (ii) contractual cooperation system with deferred payment where the value of 20 days goes to the producers and 10 days to the collector (82%). Despite this remuneration system, certain producers directly sold their milk at the price of the market to consumers in town to gain more revenue (Fig 1). The unfermented milk price was 287 XOF/L (SD 11) (€0.44) at the farm, 252 XOF/L (SD 3) (€0.38) at collection stage and 530 XOF/L (SD 24) (€0.81) at the market indeed. The exchange rate was 655.957 XOF for 1€. The monthly revenues were estimated at 83,100 XOF (SD 13,242) (€127) for producers, 88,821 XOF (SD 19,669) (€135) for collectors and 176,000 XOF (SD 22,223) (€268) for vendors.

Milk processing techniques.

Milk processing relied on secular sociocultural practices. This was observed for several key quality and safety aspects along the production chain (Fig 2): Producers and collectors (86.7% Fulani) didn’t filter (51.6%) the milk. Raw milk consumption was common due to ancestral beliefs and particularly common at producer and collector level. They stated ‘In Fulani tradition, if milk is heated, cow’s udders dry up and animals die. In addition, raw milk brings more strength.’ Furthermore, general aspects of hygiene and good manufacturing practices were lacking: Hand-washing and udder cleaning were not observed, storage (> 2 h) and transportation (> 3 h) were performed at ambient temperature, improper oil or chemical containers were used for milk storage, improper cleaning techniques of containers and 1/3 of producers observed were milking of cows under antibiotic treatment without respecting the withdrawal times.

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Fig 2. Summary chart of the production and the processing of cow milk in Korhogo along the supply chain as observed during this field study.

https://doi.org/10.1371/journal.pone.0233132.g002

Despite the traditional raw milk production, heating of milk was observed for some vendors heat-treated or fermented milk products. For heating, the majority (>90%) of vendors heated the milk to boiling temperatures. However, the heat-treated milk was still sold at ambient temperatures (31.5°C) by the majority of vendors. Only approximately 1/3 of vendors sold their milk refrigerated. Vendors kept the milk raw only on specific demand by customers.

For fermented products, all the study participants kept the milk in cleaned individual utensils for 17–19 h at home at room temperature. Only vendors produced fermented milk for sale. Producers and collectors produced fermented milk only for home consumption. The initiation of fermentation at vendor level (vendors n = 9) was based on backslopping (n = 6), the addition of commercial/industrial yogurt from heated milk (n = 1), a combination of both termed “seeding” (n = 2) or spontaneous fermentation. Approximately 1/3 of vendors declared to use spontaneous fermentation practices depending on environmental conditions. During the dry season, the warm room temperature (~29°C) [32] sustained the spontaneous fermentation and delivered FDP in about 17–19 h. In the rainy season, the room temperature was relatively low (under 25°C) [32] and fermentation took much longer, so the milk was initiated via backslopping or through the addition of an industrial yogurt as described above.

The degree of fermentation depended on the desired organoleptic properties of the final product such as flavour, texture and acidity. The fermentation can be continued for 48 to 72 h hours at ambient temperature and after an efficient churning, vendors obtained a Fulani butter called Naré. By heating, they then turned the Naré into oil (melted butter). Both Naré and oil are used for consumption and body ointment (Fig 2).

Physicochemical parameters and bacterial loads analysis of milk products

At producer level, the pH of unfermented milk was 6.9 (SD 0.1) (Table 1). After average transportation times from farms to vendors of 5h 42min at ~31.1°C, the pH was significantly reduced to 6.7 (SD 0.2) (95% CI) at vendor level in town. For FDP, the lowest pH value of 4.1 (SD 0.2) was recorded at the vendor level, but no difference was observed compared to pH values of 4.6 (SD 0.6) and 4.2 (SD 0.2) of fermented milk from producers and collectors, respectively (Table 1).

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Table 1. Physicochemical parameters and bacterial counts on M17 agar of milk products collected in Korhogo.

https://doi.org/10.1371/journal.pone.0233132.t001

The initial bacterial load of 3.3 log10 CFU mL-1 (SD 0.9) of the raw milk increased significantly (p < 0.05) while passing from producer level to collector level (5.4 log10 CFU mL-1; SD 0.9). Bacterial counts on M17 agar of FDP samples from producers (6.9 log10 CFU mL-1; SD 2.5), collectors (7.1 log10 CFU mL-1; SD 0.7) and vendors (6.4 log10 CFU mL-1; SD 2.7) were little different (Table 1). Out of the 120 milk samples collected, 10 samples (eight from vendors and two from producers) did not provide any presumptive LAB colonies on M17 agar after repetitive plating.

Phenotypic characterization of presumptive SBSEC isolates

A total of 527 bacteria were collected from M17 agar medium used to analyse 110 milk samples. Out of these isolates, 45.7% and 54.3% originated from fermented and unfermented milk samples, respectively. Out of 527 isolates, 16 isolates were excluded due to catalase-positive examination and two isolates due to fungi growth yielding 509 Gram-positive and catalase-negative isolates.

Molecular identification of presumptive SBSEC isolates to assess milk sample prevalence

The 509 isolates were processed by rep-fingerprinting to identify 494 representative isolates for processing by the SBSEC-specific PCR assay. Thereby, 178 isolates originating from 58 samples were identified as members of the SBSEC. Among these SBSEC isolates, 105 isolates originated from 29 out of 43 fermented milk samples (67.4%), while 73 isolates originated from 30 out of 67 unfermented milk samples (44.8%) (Table 2).

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Table 2. Prevalence of SBSEC bacteria and their subspecies in fresh (n = 67) and fermented milk samples (n = 43) and yielding 178 SBSEC isolates out of 286 fresh milk and 241 fermented milk isolates.

https://doi.org/10.1371/journal.pone.0233132.t002

After further representative reduction according to rep-fingerprints and sample origin (data not shown), 47 isolates were identified by partial groEL sequencing as Sii (n = 42) and Sgm (n = 5). Through clustering isolates by rep-fingerprints and association of Sii or Sgm species to individual clusters, a total of 139 and 10 isolates were assigned subsequently to Sii and Sgm, respectively. Remaining 29 SBSEC isolates were not further identified to species level. Sequence identities of groEL fragments ranged between 99.6–99.8% over 542 bp for Sii and 99.8–100% over the same 542 bp to Sgm. Two isolates displayed highest sequence identities of 95.0% and 96.4% to Sgm and Sii, respectively, but were identified as Sii via MLST (S1 Fig).

Sii were isolated from FDP (27/43) and unfermented (26/67) milk samples yielding a relative prevalence of 62.8% and 38.8%, respectively (Table 2). The highest Sii sample prevalence was observed among FDP at producer level where 18 out of 28 samples (64.3%) yielded Sii (Table 2). FDP yielded 62.8% (50.0–64.3%) Sii sample prevalence in contrast to unfermented milk samples at 38.8% (31.0–50.0%) along the three sample stages (Table 2). Overall, Sii represented 26% (n = 139, 85 from fermented milk and 54 from unfermented milk) of all 527 isolates, whereas Sgm and SBSEC of unknown subspecies represented only 5.5% and 2.3, respectively (Table 2). The lacS/lacZ adaptation was identified in 44 Sii isolates out of 90 isolates tested. Of these 44 Sii, 26 were isolated from FDP while 18 originated from unfermented milk samples. Out of 18 samples (14 FDP and four unfermented milk samples), 20 isolates were identified as non-SBSEC members harbouring the lacS/lacZ adaptation.

SBSEC population structure by MLST

The population structure of 23 Sii isolates and one Sgm isolate was analysed by SBSEC MLST (Fig 3). Isolate selection was aimed at representing different sample origins and sample types. Out of these 24 isolates, 12 Sii isolates harboured the lacS/lacZ adaptation, six of which originated from FDP and six from unfermented milk samples. The majority (18 out of 23) Sii isolates analysed in this study clustered in clade (i) (Fig 3). Thereby, clade (i) predominantly comprised isolates of CC158. CC158 in total was comprised of 39 WAD, 1 Asian dairy and 40 EAH isolates. Out of these isolates, lacS/lacZ genes were only detected in 23 WAD Sii but not among the remaining members of clade (i). Other WAD Sii isolates harbouring lacS/lacZ were only detected in clade (iv) (Fig 3). Novel clades (iv) and (v) thereby represent two lineages potentially separating from the main WAD/EAH clade (i, CC158), the presumed pathogen clade (ii, CC90) and the EAD clade (vi, CC71) (Fig 3). Out of the 23 Sii isolates analysed in this study, only Sii AR035B22.1 clustered among the presumed pathogen clade (ii, CC90).

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Fig 3. Sequence-based MLST tree of Sii isolates of dairy and human origin.

The tree was constructed using the concatenated sequences of all 10 MLST loci of all isolates and calculated using the Maximum likelihood algorithm. The tree was extracted from the combined SBSEC tree rooted to S. alactolyticus DSM 20728T (S1 Fig). Host and sources are indicated for dairy (D), human (H), animal (A) and food (F) and precision for fermented dairy products (FDP) or raw milk (raw). Clade numbers and levels were defined according to tree hierarchy. The percentage of trees in in which the associated taxa clustered together is shown next to the branches calculated from 200 bootstrap replications. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site and indicated by the bar below the graph.

https://doi.org/10.1371/journal.pone.0233132.g003

The MLST sample set of this study of 23 Sii isolates included four Sii pairs. Each pair was comprised of an isolate from FDP and one from unfermented milk samples originating from the same study participant households (AR007, AR016, AR038 and AR064). Two pairs (AR007A22.1 & AR007B22.1 and AR038A26.2 & AR038B17.1) featured identical lacS/lacZ status within each pair and clustered in closely related branches. In contrast, the other two pairs (AR064A16.2 & AR064B17.1 and AR016A23.2 & AR016B16.2) featured mixed lacS/lacZ status, minimizing the possibility for a close relationship between the individual pair members. Furthermore, two Sii isolates originated from the same sample but featured the lacS/lacZ adaptation only in one isolate (AR004A26.3), which suggests the co-existence of multiple Sii lineages within products and households.

Among the S. gallolyticus branch, the only Sgm isolate (AR061A17.2) obtained from WAD products in this study clustered in CC37 and featured the same ST-40 as an isolate previously obtained from FDP in Abidjan (S2 Fig). No Sgp, Sgg or other SBSEC species representing colonies were obtained in this study.

Discussion

This study described the milk production system, including the milk supply chain and FDP production in correlation with the prevalence of Sii/SBSEC bacteria for sub-Saharan pastoral areas on the example of Korhogo, Northern Côte d’Ivoire.

The milk supply chain in Korhogo is governed by the informal system as in the greater Sudano-Sahelian area [3335]. The clear separation of the tasks around the milk activities is common in the traditional family farm system predominated by Fulani in this area [36]. Milk trading was the dominant activity for collectors and vendors. This is in contrast to farm owners, who consider milk revenues of the existing remuneration system of as complementary means to remunerate their Fulani herdsmen. This finding is shared with other urban and peri-urban areas such as in and around Bamako (Mali) [35, 37, 38].

Milk production quantities reflected the importance of Korhogo in the dairy sector of Côte d’Ivoire and possible the Sudano-Sahelian area. The high milk amount produced per farmer per month in Korhogo of over 700 L compared to Bamako with 600 L and Abidjan (south Côte d’Ivoire) with 300 L might be related to fodder availability and advanced breeding capabilities. Combined with a larger cow population in northern Côte d’Ivoire, this area is thus considered as the dairy basin, featuring not only high production but also high consumption quantities of milk [2022, 35, 39].

Production practices in the African setting have been reported to pose risks of microbial contamination of milk [37, 40, 41]. In this study, dairy production practices were assessed to not comply with basic food hygiene standards like the Codex Alimentarius [42]. Instead, they were influenced by secular sociocultural practices around milk [37, 4345]. Multiple potential sources of contamination were identified: (i) the lack of hygienic practices such as the non-washing of the hands of the milkers and the udders of cows, the milking of cows in the pens, (ii) the permanent contact of the utensils with the ground and the faeces of animals, (iii) the massive presence of insects, (iv) the type of material used for milking, storage and transportation that are difficult to clean such as calabashes or plastic cans, (v) the non-heating of milk at producer and collector levels, (vi) the combined effects of the long storage and transportation at hot ambient temperature until delivery to sellers and customers as well as (vii) the washing system. These factors or the combination thereof seem to have favoured multiplication of Sii/SBSEC in the raw milk [39, 4447]. Particularly, the consumption of raw milk by study participants and the idea that raw milk is “better” for their health as previously reported [37] indicates a health risk [35, 48]. The systematic heating of the raw milk at vendor level contributed to a significant reduction of the bacterial load of presumptive LAB in the unfermented milk. However, cell counts of 103 CFU/mL after heat treatment suggests recontamination after heating or improper heating, supporting observations for possible non-compliance with good manufacturing practices as well as the potential re-inoculation of milk with Sii/SBSEC via utensils [10, 45]. This would also explain why Sii or SBSEC sample prevalence was not largely impacted by the heating step at vendor level.

In FDP, the lack of significant differences in bacterial counts in the supply chain is likely related to the fermentation process, which is usually yielding comparable bacterial counts for other West African FDP [9, 10]. However, randomly distributed FDP samples (n = 10) across the supply chain, where no bacteria were recovered decreased statistical significance of differences. Particularly the absence of LAB such as enterococci, lactococci and streptococci on M17 agar otherwise expected in spontaneous FDP samples with a pH <4.5 [8]. Recurrent reasons for such cell count variations might be explained by the treatment of FDP by (i) a bacteria elimination step such as sufficient heating masked by the addition of potash salts to reduce the coagulation of acid milk during the heating, (ii) the addition of acidic material such as baobab fruit that can result in a low pH, modify the predominating microbiota without the high presence of LAB or (iii) administration of antibiotics into the milk as previously reported [37, 39, 49]. Such administration of antibiotics would be a high public and animal health risk given global spread of antibiotic resistances and the prevalence of multiple zoonotic pathogens in dairy products as well as that of Sii/SBSEC [46, 5052].

In total, almost 2/3 of all FDP and nearly half of all unfermented milk samples were positive for SBSEC strains. To the best of our knowledge, no studies have so far reported the presence of Sii strains in unfermented milk [1, 810]. However, Sii could have been present in the milking containers before the milking as reported for other bacteria and may be linked to cross-contamination from FDP through utensils and handlers, or they may have originated from animals and the environment [10]. This theory is supported by our finding that Sii were present in unfermented milk samples collected in the respective containers just after the milking procedure. Detailed analysis of samples from the dairy production environment will be necessary to identify the ecological niche of Sii/SBSEC at the livestock-human-milk interface.

The current state of knowledge supported by population structure analysis via SBSEC MLST at the livestock-human-milk interface is not yet conclusive on the origin and niche of Sii. While some strains suggest the potential of contamination such as for participant households AR007 and AR038, households AR016 and AR064 suggest the opposite. Clearly, MLST analysis displayed discrimination between the main WAD/EAH and likely commensal Sii clade (i, CC158)), presumed pathogen clade (ii, CC90) as well as a newly separated dairy-adapted WAD Sii clade (iv, singletons) closer related to that of dairy-adapted EAD Sii clade (vi, CC71). This suggests the separation of the WAD Sii across these three clades and CCs This supports the previous observations regarding the high strain diversity among West African Sii isolates in contrast to Sii isolates of East Africa [14]. To which degree this implies health risks for the consumers remains unclear and will require further investigations.

The finding of high overall prevalence of SBSEC strains in spontaneously FDP and unfermented milk samples adds the area of Korhogo in northern Côte d’Ivoire to the places of confirmed Sii and SBSEC presence in West Africa in addition to Mali and Abidjan area of Côte d’Ivoire [9, 10]. This high overall prevalence might contribute to livestock and livestock primary product contact and rural habitat as indicators for Sii faecal carriage in humans [19]. Given the transhumant populations and cross-border trade in northern Côte d’Ivoire, this supports the postulated hypothesis of an even wider distribution of Sii across sub-Saharan Africa [1]. Relating to microbiota data from similar raw and fermented dairy products in Southern Côte d’Ivoire, Mali, Kenya, Sudan and Somalia [710], this suggest that a similar role of Sii and Sgm in the dairy fermentation process could be also be expected for the area of Korhogo. This supports the urge to assess the ecology of these bacteria in the dairy and connected livestock environment in West Africa and elucidate their role in dairy fermentations in comparison to S. thermophilus.

Conclusion

This study provided the first dairy production system assessment and Sii/SBSEC prevalence determination for Northern Côte d’Ivoire, the main dairy producing area of Côte d’Ivoire. The dairy production system featured limited compliance with good manufacturing practices. SBSEC bacteria, predominantly Sii, were found to be present in around 2/3 of FDP and over 1/3 of unfermented milk samples. Sii were present as dairy-adapted and non-adapted variants. This adds northern Côte d’Ivoire and likely further areas in West Africa connected via cross-border trading routes to the areas of confirmed Sii presence in dairy products. Therefore, Sii and SBSEC members likely have an impact on the diet of dairy consumers across multiple countries of sub-Saharan Africa. Furthermore, Sii population structure and presence of dairy adaptation marker genes suggest ongoing diversification and evolution into a novel dairy-adapted West African Sii lineage. This novel lineage formed in addition to previously described CC158 (clade (i)) of WAD and EAH Sii of presumed commensal origin and that of CC90 harbouring mainly human blood isolates (clade (ii)). This high diversity and shared relationships with potential commensal as well as presumed pathogenic clades support in-depth investigations of the ecology and public health risks of Sii/SBSEC lineages in the dairy environment and their role in comparison to S. thermophilus.

Supporting information

S1 Fig. Sequence-based MLST tree of SBSEC of dairy, animal and human origin.

The tree was constructed using the concatenated sequences of all 10 MLST loci of all SBSEC isolates and calculated using the Maximum likelihood algorithm. The tree was rooted to S. alactolyticus DSM 20728T. The main species clades are indicated for S. infantarius subsp. infantarius, S. lutetiensis and the S. gallolyticus branch with geographic origin of isolates specifically indicated for West Africa (WA) and East Africa (EA). The percentage of trees in which the associated taxa clustered together is shown next to the branches calculated from 200 bootstrap replications. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site and indicated by the bar below the graph.

https://doi.org/10.1371/journal.pone.0233132.s001

(TIF)

S2 Fig. Sequence-based MLST tree of the S. gallolyticus branch with isolates of dairy and human origin.

The tree was constructed using the concatenated sequences of all 10 MLST loci of all isolates and calculated using the Maximum likelihood algorithm. The tree was extracted from the combined SBSEC tree rooted to S. alactolyticus DSM 20728T (S1 Fig). Host and sources are indicated for animal (A), dairy (D), human (H), West African Dairy (WAD), East African Dairy (EAD) and fermented dairy products (FDP). Clade numbers and levels were defined according to tree hierarchy. The percentage of trees in in which the associated taxa clustered together is shown next to the branches calculated from 200 bootstrap replications. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site and indicated by the bar below the graph.

https://doi.org/10.1371/journal.pone.0233132.s002

(TIF)

Acknowledgments

The authors acknowledge the ERAfrica Safe Dairy consortium and particularly Wambui Kogi-Makau, Esther Schelling, Jakob Zinsstag, and Christophe Lacroix for their support to the overall project. We acknowledge support received from the Laboratoire National d'Appui au Développement Agricole, LANADA in Korhogo, Côte d’Ivoire to initiate and conduct the field study. We thank Jasmina Saric for reviewing the manuscript.

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