The deletion of exons 9 to 12 of BRCA1 (9–12 del BRCA1) is considered a founder mutation in the Mexican population. We evaluate the usefulness of the target detection of 9–12 del BRCA1 as the first molecular diagnostic strategy in patients with Hereditary Breast and Ovarian Cancer (HBOC). We performed the genetic assessment of 637 patients with suspected HBOC. The region corresponding to the breakpoints for the 9–12 del BRCA1 was amplified by polymerase chain reaction (PCR). An analysis of the clinical data of the carriers and non-carriers was done, searching for characteristics that correlated with the deletion. The 9–12 del BRCA1 was detected in 5% of patients with suspected HBOC (30/637). In patients diagnosed with ovarian cancer, 13 of 30 were 9–12 del BRCA1 carriers, which represents 43%. We found a significant association between the 9–12 del BRCA1 carriers with triple negative breast cancer and high-grade papillary serous ovarian cancer. We concluded that the detection of the 9–12 del BRCA1 is useful as a first molecular diagnostic strategy in the Mexican population. In particular, it shortens the gap in genetic assessment in patients with triple negative breast cancer and ovarian cancer.
Citation: Fragoso-Ontiveros V, Velázquez-Aragón JA, Nuñez-Martínez PM, de la Luz Mejía-Aguayo M, Vidal-Millán S, Pedroza-Torres A, et al. (2019) Mexican BRCA1 founder mutation: Shortening the gap in genetic assessment for hereditary breast and ovarian cancer patients. PLoS ONE 14(9): e0222709. https://doi.org/10.1371/journal.pone.0222709
Editor: Alvaro Galli, CNR, ITALY
Received: February 18, 2019; Accepted: September 5, 2019; Published: September 23, 2019
Copyright: © 2019 Fragoso-Ontiveros et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information files.
Funding: The authors received no specific funding for this work.
Competing interests: The authors have declared that no competing interest exist.
The main genes associated with hereditary breast and ovarian cancer are BRCA1 and BRCA2, tumour suppressor genes whose germline mutations confer a high risk for the development of breast cancer (45–65%), and ovarian cancer (11–40%) [1–4].
The majority of pathogenic mutations in BRCA1/BRCA2 genes are point mutations (80%). In 10 to 15% there are large rearrangements, such as deletions or duplications of exons [5–7]. In both genes, more than 3,000 mutations have been reported [8–9]. Within these, mutations with a founder effect have been described in populations such as the Ashkenazi Jews, who are considered a genetically homogenous population [10–11]. Despite the heterogeneity of the ancestry of Latin American populations, mutations with a founder effect have also been described in Brazil (BRCA1 5382insC and BRCA2 c.156_157insAlu) , and Colombia (BRCA1 3450del4, A1708E, and BRCA2 3034del4) . In Mexico, a mutation with founder effect has been reported, the deletion of exons 9 to 12 of BRCA1 (9–12 del BRCA1) , which represents up to 30% of the total of the mutations identified [15–16]. This mutation is common in patients with ovarian cancer, in which the overall frequency of mutations in BRCA1/BRCA2 is 28%, which is mainly attributable to this founder effect .
The detection of founder mutations in specific populations can be considered as a cost-effective option, making molecular diagnosis more accessible, with the implications of diagnosis, treatment, prognosis, and prevention that this entails .
In 2014, the Society of Gynaecologic Oncology (SGO) recommended genetic counseling and molecular tests to all patients with ovarian cancer . The possibility of personalising the treatment according to the mutational state, as occurs in the choice of treatments with platinum compounds and poly (ADP-ribose) polymerase 1 (PARP1) inhibitors in patients with ovarian cancer, has extended the recommendation to most international guidelines [19–20]. However, important barriers have been found to implement the recommendation worldwide. The Latin American region, in particular, faces limitations of access to molecular analysis due to its high cost [17,21].
In this study, we have presented the results of a targeted search for 9–12 del BRCA1 in a group of 764 patients and relatives cared for at the first Hereditary Cancer Clinic in Mexico. We have provided an overview of the main clinical characteristics in correlation with 9–12 del BRCA1, highlighting the diagnosis of triple-negative breast cancer and high-grade papillary ovarian cancer. Likewise, a targeted search for a founder mutation might be the most cost-effective molecular diagnosis strategy in patients with suspected hereditary breast and ovarian cancer (HBOC) in Mexico.
Materials and methods
A total of 764 genomic DNA samples were included, for targeted detection of the deletion of exons 9 to 12 of BRCA1. Of the total DNA samples, 637 corresponded to oncological patients assessed at the Hereditary Cancer Clinic or the National Cancer Institute (Mexico), due to the suspicion of hereditary breast and ovarian cancer (HBOC). The clinical suspicion relies on the personal history (early age of onset; tumor phenotype; bilateral presentation), and family history (relatives with associated tumors; degree of relationship), according to criteria established in the Genetic/Familial High-Risk Assessment: Breast and Ovarian of the National Comprehensive Cancer Network (NCCN) guidelines, version 2.2017 (https://www.nccn.org/). All patients received genetic counselling and medical follow-up, by a certified medical geneticist.
We also included 127 relatives at risk to be carriers of 9–12 del BRCA1, for whom pre-symptomatic detection was performed. All family members received the corresponding pre- and post-test genetic counselling by a medical geneticist.
All patients and relatives gave their informed consent for inclusion before they participated in the study. The study was conducted in accordance with the Declaration of Helsinki, and Institutional Review Board (Comité de Ética en Investigación, Instituto Nacional de Cancerología, Mexico: 12 CEI0901411) approval was obtained (CEI/1036/16).
For all patients enrolled, 3 ml of peripheral blood were collected. Peripheral blood DNA was extracted with the Wizard® Genomic DNA Purification Kit (Promega), following manufacturer’s instructions. DNA concentration was quantified with NanoDrod 2000® (Thermo). Integrity and purity of nucleic acids was verified by agarose gel electrophoresis and spectrophotometry, respectively.
Amplification polymerase chain reaction (PCR) of 9–12 del BRCA1
Region corresponding to the sites of rupture of the deletion was amplified (exons 9 to 12, BRCA1) by means of PCR. Weitzel et al., (2007) reported a similar method . However, the assay design and the primers are different. Briefly, the fragments corresponding to the normal and mutated alleles were amplified with the oligonucleotides B01del9-12, B02del9-12 and B03del9-12. The PCR endpoint was performed in a GeneAmp PCR System 9700, (Applied Biosystems) in a volume of 30 μL, 150 ng of gDNA and a final concentration of 1X DreamTaq Buffer (Thermo Scientific), 0.25 mM of dATP, dTTP and dCTP, 0.2 mM of dGTP, (Thermo Scientific) with 0.63 mM of -N7-dGTP, 7-deaza-dGTP (SIGMA), 0.33 μM of each oligonucleotide and 0.06 U of DreamTaq DNA Polymerase (Thermo Scientific). The reaction conditions were: 1) 94°C 5min; 2) 94°C for 30 sec, 50°C for 30 sec, 72°C for 35 sec, 10 cycles; 3) 94°C for 30 sec, 53°C for 40 sec, 72°C for 40 sec, 25 cycles; 4) 72°C for 10 min; 5). Subsequently, electrophoresis was performed on 1.5% agarose gel and 0.5 X of Tris-borate-EDTA (ethylenediaminetetraacetic acid) buffer (ChemCruz). The presence of bands corresponding to the mutated allele in the amplified samples was verified (S1 Fig). A second PCR was performed with the mutated allele samples using only the oligonucleotides B01del9-12 and B03del9-12 with the same amplification conditions. The amplification was corroborated by electrophoresis in 1.5% agarose gel and 0.5 X of Tris-borate-EDTA buffer (ChemCruz). To enhance the reproducibility of our results, a detail laboratory protocol would be found at protocols.io: doi.org/10.17504/protocols.io.4t8gwrw.
The presence of 9–12 BRCA1 deletion was corroborated through Sanger sequencing. A volume of 5 ul of the amplified product was purified with 2 μL of ExoSAP-IT PCR Product Cleanup (Affymetrix) under the conditions indicated by the manufacturer. The sequencing reaction was performed with a BigDye Terminator v3-1 Cycle Sequencing Kit (Applied Biosystems), as indicated by the manufacturer. Then, the product was labelled with a BigDye XTerminator Purification Kit (Thermo Fisher Scientific). It was analysed in 3500 Genetic Analysers (Applied Biosystems). The presence of the deletion was corroborated by aligning the sequence obtained with the sequence in the FASTA format of the BRCA1 gene NG_005905.2 RefSeqGene (http://www.ncbi.nlm.nih.gov, NCBI GenBank) through the eBioX 1.6 Beta programme 1 Build 26/1.5.1).
The statistical analysis was carried out using the IBM SPSS Statistics v.24 programme. After the analysis based on descriptive statistics, group comparison methods were used for the analysis of the clinical characteristics of patients carrying the 9–12 BRCA1, with respect to non-carriers. For this effect, two-sided Fisher’s exact test was performed. A "p" value of 0.05 was considered significant. Correction for multiple comparisons was performed with the Benjamini-Hochberg false discovery rate method.
In patients with suspected HBOC syndrome, 30 (5%) were identified as carriers of the 9–12 del BRCA1 (Table 1).
As relevant clinical characteristics of HBOC carriers of the 9–12 del BRCA1, all were female. The mean age at the first cancer diagnosis was 43 years (SD 14.63), which did not have a significant difference with non-carrier patients.
In relation to oncological diagnoses, 18 patients had a history of breast cancer. In 12 of them, the condition was unilateral, while in 6 it was bilateral (2 synchronic, 4 metachronic). The histology of the tumors was predominantly of the infiltrating ductal carcinoma (23/24).
Regarding the tumour phenotype, in 7 of the patients with unilateral breast cancer and in all the patients with bilateral breast cancer, the tumors were triple negative. The remaining patients presented luminal A or B tumors.
In patients with ovarian cancer, 9–12 del BRCA1 was detected in 43% (13/30) of the cases. All tumors had an epithelial component, highlighting high-grade serous epithelial tumours (10/13); the rest of the histologies corresponded to mixed tumours (3/13).
It should be noted that in two of the carriers, the presence of multiple cancer diagnoses were documented. In the first case, it was a patient with ovarian cancer and metachronous breast cancer. In the second case, the patient had a history of cervical cancer and metachronous bilateral breast cancer.
In relation to family history, 80% of the patients had cancer background, highlighting breast cancer in first- and second-degree relatives. In six of the carriers there was not family cancer history, so the suspicion of HBOC lies in age at diagnosis or tumor phenotype. There was not information available on the health status of their family in two of the patients.
In the case of the 127 family members included, they extended the information about their genealogy (S1 Table). Of them, 55 (43%) were identified as asymptomatic carriers of 9–12 del BRCA1. Of the patients identified as carriers, most of then were female (36/55).
In the pedigree, 100% of the relatives identified as asymptomatic carriers, had a positive history of at least one first- or second-degree relative with breast cancer. The average of cancer family members was three.
Additionally, the association between carry the 9–12 deletion BRCA1 with different clinical features such as age at diagnosis, type of cancer, tumor phenotype, was analyzed among patients, carriers and non-carriers.
The triple negative tumour phenotype in breast cancer was statistically associated to 9–12 del BRCA1 carriers, when compared with the group of non-carriers (73% versus 21%, p = 0.0005; two-sided Fisher's exact test). Also, an association was found with diagnosis of ovarian cancer, particularly high-grade papillary serous histology (p = 0.0004; two-sided Fisher's exact test). Both associations remain significant after adjustment for multiple comparisons by the Benjamini-Hochberg method.
The identification of patients with HBOC, allows the personalisation of oncological treatment and prognosis, as well as the establishment of risk reduction measures for cancer, both in patients and in family members, constituting the strategies of greater effectiveness in cancer prevention . Therefore, it has become a daily practice in the multidisciplinary care of patients with breast and ovarian cancer .
Germline mutations of the BRCA1 and BRCA2 genes are the main etiological component of HBOC [1–4]. Genomic large rearrangements (LGRs), such as exon deletions or duplications, are defined as those alterations that involve more than 500 kb of DNA . For the identification of LGRs, the most widely used technique is the Multiplex Ligation Probe Assay (MPLA) .
So far, more than 130 LGRs have been identified in the BRCA genes . Most of the LGRs are in BRCA1, due to the large amount of Alu repeats that comprise it, which makes it susceptible to errors in recombination. In populations where founder effects exist, the first molecular diagnosis consists of the directed search for it as the first option due to its frequency. This strategy allows the optimisation of time and economic resources [17, 21].
Large rearrangements, such as founder mutations in the BRCA genes, have been reported predominantly in the Caucasian population (Germany, Greece, Spain) [27–29]. In the Dutch population, two large genomic rearrangements, the deletion of 3.8 kb of exon 13, and the deletion of 510 bp of exon 22, represent approximately 25% of the mutations identified in the cases of hereditary breast cancer . In Latin America, the 9–12 del BRCA1 represents the first large genomic rearrangement with a founder effect in the region .
Weitzel et al. identified 9–12 del BRCA1 for the first time in 2005, studying a population at risk of hereditary cancer of Latin American origin (predominantly of Mexican ancestry) residing in the United States of North America . In 2007, the same group of researchers reported the molecular characterisation of the deletion . A later survey in the Colombian population documented its absence by looking for it in a targeted way in 538 patients with suspected HBOC . The above data is considered part of the evidence of the founder characteristic in the Mexican population.
A year later, in 2013, by genotyping carriers for 9–12 del BRCA1 with Mexican ancestry and residents predominantly in the southern United States of North America, it was estimated that the mutation originated 74 generations ago or 1,480 years earlier .
Until that date, a limitation of the studies that involved the diagnosis of 9–12 del BRCA1 was due to the absence of data in a purely Mexican population. Therefore, in 2014, Torres-Mejia et al., performed a molecular analysis of recurrent mutations in BRCA1/BRCA2 in 810 unselected breast cancer patients, residents of three Mexican cities (Mexico City, Veracruz and Monterrey). The 9–12 del BRCA1 was the most common mutation, representing 22% of the mutations identified .
Two other publications with projects conducted in the Mexican population reported relevant data regarding 9–12 del BRCA1. In the first study, which involved patients with breast and ovarian cancer, without suspicion of hereditary cancer, 9–12 del BRCA1 represented 33% of the total mutations identified, 35% in the subgroup of patients with ovarian cancer and 29% in that of patients with breast cancer, constituting the most frequent mutation . In the second study, whose objective was the molecular analysis of the BRCA genes in 190 patients with triple negative breast cancer diagnosed before the age of 50 years, the 9–12 of the BRCA1 accounted for 41% of mutations identified .
Recently, the world's largest study of prevalence of BRCA1 and BRCA2 mutations has been published. This study included 29,700 BRCA1/BRCA2 mutation carriers from 49 countries, distributed across 6 continents. In that report, the 9–12 del BRCA1 is the most frequent mutation in Mexico, and the fourth most prevalent in Hispanic/Latino ethnicity .
Our results show a 5% frequency of 9–12 del BRCA1 when its directed search is applied as the first molecular diagnostic strategy to a group of 637 patients with clinical suspicion of HBOC.
Previous studies of the Mexican population show that 9–12 del BRCA1 represents between 23 and 41% of the total number of mutations in BRCA1 (Table 2.).
In our study, the 9–12 del BRCA1 represents the 30.92%, given that 67 point mutations were identified in BRCA1 by sequencing (data not shown). It should be noted that if the study population is stratified by characteristics, such as the triple negative phenotype, the proportion that represents 9–12 del of the total BRCA1 mutations is higher.
Implementing the targeted detection as a first step represents obtaining an outcome by an accessible, efficient and effective technique, in economic terms (less than $10), and in waiting time (less than 1 week). Thus, the subgroup of patients identified as carriers no longer required other testing, such as sequencing/MLPA, which is helpful due to the challenges of accessibility that had Latin America [17,36,37]. In this context in Mexico, the cost of a cancer susceptibility genes molecular test ranges between $ 500 and $ 5,000, according to different commercial laboratories. A Mexican family receives an average of 88 pesos per day (less than $ 5), evidencing the accessibility problem that we face . Having a cost-effective strategy for a founder mutation related to HBOC (with a cost less than $ 10), could shorten that gap, and even make it accessible to be cover by the public health system. Additionally, it was possible to benefit 127 asymptomatic individuals, identifying 43% as carriers in the context of a predictive study.
Because LGRs have been considered particularly penetrating mutations, being associated with greater cancer risks, and even certain "high-risk characteristics" , we intentionally sought their correlation with the clinical data present in the population studied. In this way, the correlation of carriers of 9–12 del BRCA1 with triple negative breast cancer was found, as well as high-grade papillary serous ovarian cancer. These data are in congruence with previous data reported in our institution since the 9–12 del BRCA1 was found in 9% of 190 patients with triple negative breast cancer, under 50 years of age, not selected for their family history . Similarly, the 9–12 del BRCA1 was identified in 9% of 92 unrelated patients with ovarian cancer of different histologies .
For this same reason, one of the main limitations of our study is that it mainly reflects the population of the centre of the country (Fig 1). Despite being the national cancer institution, the territorial extension of the country (1,964 million km²) and other migratory, social and cultural phenomena, limit the generalisation that can be made of the distribution of the founder mutation 9–12 del BRCA1 in our population. More studies will be needed from other regions of the country to determine whether their behaviour remains constant or has considerable variation.
However, one of our contributions lies in the possibility of making molecular testing more accessible to our population, considering as a first line of diagnosis the detection for 9–12 del BRCA1 in all patients with suspected HBOC (Fig 2). In this way, we propose that all patients with suspected HBOC, who were born in the central region of our country, begin their diagnostic approach with the targeted detection of 9–12 del BRCA1. If the result is negative, it will be possible to continue with the sequencing and search for large rearrangements (by MLPA, for example) in the associated susceptibility genes.
We emphasise the possibility of its finding in patients with high-grade papillary serous ovarian cancer and triple negative breast cancer. Particularly in ovarian cancer and in accordance with international recommendations for universal genetics assessment, our proposal guarantees access to a first molecular screening line, which is potentially feasible throughout the country. Likewise, it allows the identification of those patients who may be susceptible to a targeted treatment, such as PARP1 inhibitors, which currently have national approval for the treatment of recurrent ovarian cancer.
S1 Fig. Targeted detection for the 9–12 del BRCA1.
We thank LXII and LXIII Legislature of the Congress of the Union of Mexico, for their support.
- 1. Brody LC, Biesecker BB. Breast cancer susceptibility genes BRCA1 and BRCA2. Medicine. 1998; 77: 208–26. pmid:9653432
- 2. King MC, Marks JH, Mandell JB; New York Breast Cancer Study Group. Breast and ovarian cancer risks due to inherited mutations in BRCA1 and BRCA2. Science. 2003; 302: 643–6. pmid:14576434
- 3. Narod SA, Foulkes WD. BRCA1 and BRCA2: 1994 and beyond. Nat Rev Cancer. 2004; 4: 665–76. pmid:15343273
- 4. Mavaddat N, Peock S, Frost D, Ellis S, Platte R, Fineberg E, et al. Cancer risks for BRCA1 and BRCA2 mutation carriers: results from prospective analysis of EMBRACE. J Natl Cancer Inst. 2013; 105: 812–22. pmid:23628597
- 5. Palma MD, Domchek SM, Stopfer J, Erlichman J, Siegfried JD, Tigges-Cardwell J, et al. The relative contribution of point mutations and genomic rearrangements in BRCA1 and BRCA2 in high-risk breast cancer families. Cancer Res. 2008; 68: 7006–14. pmid:18703817
- 6. Rebbeck TR, Mitra N, Wan F, Sinilnikova OM, Healey S, McGuffog L, et al. Association of type and location of BRCA1 and BRCA2 mutations with risk of breast and ovarian cancer. JAMA. 2015; 313: 1347–61. pmid:25849179
- 7. Rebbeck TR, Friebel TM, Friedman E, Hamann U, Huo D, Kwong A, et. al. Mutational spectrum in a worldwide study of 29,700 families with BRCA1 or BRCA2 mutations. Hum Mutat. 2018; 39: 593–620. pmid:29446198
- 8. Landrum MJ, Lee JM, Benson M, Brown GR, Chao C, Chitipiralla S, et al. ClinVar: improving access to variant interpretations and supporting evidence. Nucleic Acids Res. 2018; 46: D1062–D1067. pmid:29165669
- 9. Szabo C, Masiello A, Ryan JF, Brody LC. The breast cancer information core: database design, structure, and scope. Hum Mutat. 2000; 16: 123–31. pmid:10923033
- 10. Roa BB, Boyd AA, Volcik K, Richards CS. Ashkenazi Jewish population frequencies for common mutations in BRCA1 and BRCA2. Nat Genet. 1996; 14: 185–7. pmid:8841191
- 11. Gabai-Kapara E, Lahad A, Kaufman B, Friedman E, Segev S, Renbaum P, et al. Population-based screening for breast and ovarian cancer risk due to BRCA1 and BRCA2. Proc Natl Acad Sci U S A. 2014; 111: 14205–10. pmid:25192939
- 12. Gomes MC, Costa MM, Borojevic R, Monteiro AN, Vieira R, Koifman S, et al. Prevalence of BRCA1 and BRCA2 mutations in breast cancer patients from Brazil. Breast Cancer Res Treat. 2007; 103: 349–53. pmid:17063270
- 13. Torres D, Rashid MU, Gil F, Umana A, Ramelli G, Robledo JF, et al. High proportion of BRCA1/2 founder mutations in Hispanic breast/ovarian cancer families from Colombia. Breast Cancer Res Treat. 2007; 103: 225–32. pmid:17080309
- 14. Weitzel JN, Lagos V, Blazer KR, Nelson R, Ricker C, Herzog J, et al. Prevalence of BRCA mutations and founder effect in high-risk Hispanic families. Cancer Epidemiol Biomarkers Prev. 2005; 14: 1666–1671. pmid:16030099
- 15. Villarreal-Garza C, Alvarez-Gómez RM, Pérez-Plasencia C, Herrera LA, Herzog J, Castillo D, et al. Significant clinical impact of recurrent BRCA1 and BRCA2 mutations in Mexico. Cancer. 2015; 121: 372–8. pmid:25236687
- 16. Villarreal-Garza C, Weitzel JN, Llacuachaqui M, Sifuentes E, Magallanes-Hoyos MC, Gallardo L, et al. The prevalence of BRCA1 and BRCA2 mutations among young Mexican women with triple-negative breast cancer. Breast Cancer Res Treat. 2015; 150: 389–94. pmid:25716084
- 17. Ossa CA, Torres D. Founder and Recurrent Mutations in BRCA1 and BRCA2 Genes in Latin American Countries: State of the Art and Literature Review. Oncologist. 2016; 21: 832–9. pmid:27286788
- 18. Lancaster JM, Powell CB, Chen LM, Richardson DL. SGO Clinical Practice Committee. Society of Gynecologic Oncology statement on risk assessment for inherited gynecologic cancer predispositions. Gynecol Oncol. 2015; 136: 3–7. pmid:25238946
- 19. Daly MB, Pilarski R, Berry M, Buys SS, Farmer M, Friedman S, et al. NCCN Guidelines Insights: Genetic/Familial High-Risk Assessment: Breast and Ovarian, Version 2.2017. J Natl Compr Canc Netw. 2017; 15: 9–20. pmid:28040716
- 20. Paluch-Shimon S, Cardoso F, Sessa C, Balmana J, Cardoso MJ, Gilbert F, et al. Prevention and screening in BRCA mutation carriers and other breast/ovarian hereditary cancer syndromes: ESMO Clinical Practice Guidelines for cancer prevention and screening. Ann Oncol. 2016; 27: v103–v110. pmid:27664246
- 21. Chavarri-Guerra Y, Blazer KR, Weitzel JN. Genetic Cancer Risk Assessment for Breast Cancer in Latin America. Rev Invest Clin. 2017; 69: 94–102. pmid:28453507
- 22. Weitzel JN, Lagos VI, Herzog JS, Judkins T, Hendrickson B, Ho JS, et al. Evidence for common ancestral origin of a recurring BRCA1 genomic rearrangement identified in high-risk Hispanic families. Cancer Epidemiol Biomarkers Prev. 2007; 16: 1615–20. pmid:17646271
- 23. Wunderle M, Olmes G, Nabieva N, Häberle L, Jud SM, Hein A, et al. Risk, Prediction and Prevention of Hereditary Breast Cancer—Large-Scale Genomic Studies in Times of Big and Smart Data. Geburtshilfe Frauenheilkd. 2018; 78: 481–492. pmid:29880983
- 24. Moyer VA, U.S. Preventive Services Task Force. Risk assessment, genetic counseling, and genetic testing for BRCA-related cancer in women: U.S. Preventive Services Task Force recommendation statement. Ann Intern Med. 2014; 160: 271–81. pmid:24366376
- 25. Gu W, Zhang F, Lupski JR. Mechanisms for human genomic rearrangements. Pathogenetics. 2008; 1: 4. pmid:19014668
- 26. Schouten JP, McElgunn CJ, Waaijer R, Zwijnenburg D, Diepvens F, Pals G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res. 2002; 30: e57. pmid:12060695
- 27. Palanca S, de Juan I, Perez-Simó G, Barragán E, Chirivella I, Martínez E, et al. The deletion of exons 3–5 of BRCA1 is the first founder rearrangement identified in breast and/or ovarian cancer Spanish families. Fam Cancer. 2013; 12: 119–23. pmid:23117300
- 28. Apostolou P, Pertesi M, Aleporou-Marinou V, Dimitrakakis C, Papadimitriou C, Razis E, et al. Haplotype analysis reveals that the recurrent BRCA1 deletion of exons 23 and 24 is a Greek founder mutation. Clin Genet. 2017; 91: 482–487. pmid:27357818
- 29. Hartmann C, John AL, Klaes R, Hofmann W, Bielen R, Koehler R, et al. Large BRCA1 gene deletions are found in 3% of German high-risk breast cancer families. Hum Mutat. 2004; 24: 534.
- 30. Petrij-Bosch A, Peelen T, van Vliet M, van Eijk R, Olmer R, Drüsedau M, et al. BRCA1 genomic deletions are major founder mutations in Dutch breast cancer patients. Nat Genet. 1997; 17: 341–5. pmid:9354803
- 31. Torres D, Rashid MU, Colombian Breast Cancer Study Group (COLBCS), Seidel-Renkert A, Weitzel JN, Briceno I, et al. Absence of the BRCA1 del (exons 9–12) mutation in breast/ovarian cancer families outside of Mexican Hispanics. Breast Cancer Res Treat. 2009; 117: 679–81. pmid:19333752
- 32. Weitzel JN, Clague J, Martir-Negron A, Ogaz R, Herzog J, Ricker C, et al. Prevalence and type of BRCA mutations in Hispanics undergoing genetic cancer risk assessment in the southwestern United States: a report from the Clinical Cancer Genetics Community Research Network. J Clin Oncol. 2013; 31: 210–6. pmid:23233716
- 33. Torres-Mejía G, Royer R, Llacuachaqui M, Akbari MR, Giuliano AR, Martínez-Matsushita L, et al. Recurrent BRCA1 and BRCA2 mutations in Mexican women with breast cancer. Cancer Epidemiol Biomarkers Prev. 2015; 24: 498–505. pmid:25371446
- 34. Quezada Urban R, Díaz Velásquez CE, Gitler R, Rojo Castillo MP, Sirota Toporek M, Figueroa Morales A, et al. Comprehensive Analysis of Germline Variants in Mexican Patients with Hereditary Breast and Ovarian Cancer Susceptibility. Cancers (Basel). 2018 Sep 27;10(10). pii: E361.
- 35. Zayas-Villanueva OA, Campos-Acevedo LD, Lugo-Trampe JJ, Hernández-Barajas D, González-Guerrero JF, Noriega-Iriondo MF, et al. Analysis of the pathogenic variants of BRCA1 and BRCA2 using next-generation sequencing in women with familial breast cancer: a case-control study. BMC Cancer. 2019 Jul 22;19(1):722. pmid:31331294
- 36. Strasser-Weippl K, Chavarri-Guerra Y, Villarreal-Garza C, Bychkovsky BL, Debiasi M, Liedke PE, et al. Progress and remaining challenges for cancer control in Latin America and the Caribbean. Lancet Oncol. 2015; 16: 405–38.
- 37. Lazcano-Ponce E, Mohar-Betancourt A, Meneses-García A, Hernández-Ávila M. Cancer burden in Mexico: urgent challenges to be met. Salud Publica Mex. 2016; 58: 101–3. pmid:27557368
- 38. Encuesta Nacional de Ocupación y Empleo, mayores de 15 años, en México 2018. Instituto Nacional de Estadística y Geografía (INEGI): www.inegi.org.mx/programas/enoe/15ymas/
- 39. James PA, Sawyer S, Boyle S, Young MA, Kovalenko S, Doherty R, et al. Large genomic rearrangements in the familial breast and ovarian cancer gene BRCA1 are associated with an increased frequency of high risk features. Fam Cancer. 2015; 14: 287–95. pmid:25678442