The present study recorded the population of the goby fish (Perciformes: Gobiidae), Eutaeniichthys cf. gilli Jordan & Snyder, 1901, from the tunnel burrowed by the mud shrimp Austinogebia edulis Ngo-Ho and Chan, 1992 in a mudflat in Shengang and Wangong of Changhua County, western Taiwan. This finding is not only a new record of the genus in Taiwan, it is also the first record of this species in a mudflat near an industrial park. In total, 56 individuals of E. cf. gilli were collected from June 2016 to September 2018. Morphological traits of males and females were measured. The resin casting method trapped bodies of E. cf. gilli that were present in the tunnel burrow and proved that the fish inhabits burrows of the mud shrimp A. edulis. In addition, a species of snapping shrimp was also found in the same tunnel. Symbiotic interaction may occur between E. cf. gilli, A. eduli and the snapping shrimp. The China Coastal Current (CCC) runs along the coastlines of Japan, Korea, China, and reaches western Taiwan during the northeast monsoon period. The CCC, therefore, might play an important role in the biogeographic distribution of E. cf. gilli in the western Pacific Ocean. Since E. cf. gilli is listed in the Red List as an endangered species of Japan for many years, Taiwan waters may provide a refuge for this fish species warranting a broader investigation. Since Taiwan is some distance away from the previously recorded locations in Japan, Korea, the Yellow Sea, and the Bohai Sea, a phylogenic analysis is warranted for population and species differentiation in the future.
Citation: Tseng L-C, Huang S-P, Das S, Chen I-S, Shao K-T, Hwang J-S (2019) A slender symbiotic goby hiding in burrows of mud shrimp Austinogebia edulis in western Taiwan. PLoS ONE 14(7): e0219815. https://doi.org/10.1371/journal.pone.0219815
Editor: Ismael Aaron Kimirei, Tanzania Fisheries Research Institute, UNITED REPUBLIC OF TANZANIA
Received: May 1, 2019; Accepted: July 2, 2019; Published: July 22, 2019
Copyright: © 2019 Tseng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The specimens of fish after identification were deposited at the Biodiversity Research Center, Academia Sinica, Taipei, Taiwan. All data generated during the study are interpreted in the manuscript.
Funding: This work was supported by the Ministry of Science and Technology (MOST) of Taiwan is acknowledged through grants MOST 106-2811-M-019-004 and MOST 107-2811-M-019-004 to L.-C. Tseng, as well as the grants MOST 106-2621-M-019-001 and MOST 107-2621-M-019-001 to J.-S. Hwang. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In order to develop the industry and extend land use towards the ocean, the use of limited land, large-scale land reclamation was carried out in wetlands along the western coast of Taiwan . Such man-made reclamations change the coastal environment, causing habitat fragmentation, reducing the resources of prey, and causing contaminations through pollution [2, 3]. They will, therefore, impact the coastal ecosystems and endanger organisms that inhabit the area. Examples include endangered populations of the Chinese white dolphin, Sousa chinensis (Osbeck, 1765) , changes in the breeding habitat of little tern, Sterna albifrons , accumulation of heavy metals in soldier crab, Mictyris brevidactylus , and decrease in the density of mud shrimp, Austinogebia edulis (Ngoc-Ho & Chan, 1992; synonymy: Upogebia edulis Ngoc-Ho & Chan, 1992) .
The mud shrimp, A. edulis (Fig 1), is an economic species and provides a traditional food in central western Taiwan . It collects the fine sediment particles to build its burrow and perhaps utilizes the organic materials from the burrow wall . Reports found the symbiotic alpheid shrimp Chelomalpheus crangonus Anker, Jeng & Chan, 2001 , and the squillid shrimp Cloridopsis scorpio (Latreille, 1828)  from the burrows of A. edulis. Previous studies showed the bio-accumulation of different heavy metals takes place in the shrimp from nearby wetlands of Changhua Coastal Industrial Park . A recent report found that they are sensitive to cadmium pollution . As yet, the biology and ecology of the mud shrimp A. edulis remains unstudied .
Specimen photograph of Eutaeniichthys cf. gilli (ASIZP0080765) (a), the scale bar represents 10 mm; and its symbiotic mud shrimp species (Austinogebia edulis) (b). Standard length for E. cf. gilli is 31.2 mm; carapace length for mud shrimp is 18.1 mm.
During a program of biodiversity survey and investigation, the biology of the mud shrimp, A. edulis, the shrimp was found periodically from June 2016 to June 2018 in western Taiwan. Some small slender gobies were found from the tunnel burrowed by A. edulis. Subsequently, these gobies were identified as Eutaeniichthys gilli Jordan & Snyder, 1901 based on their specific features (Fig 1A). This species is a new record of the genus in Taiwan. Previous literatures revealed that the distribution of E. gilli was mostly in the northern waters of the East China Sea, e.g., Japan, Korea, Yellow Sea and Bohai Sea. Because a thorough phylogenetic analysis is lacking as yet, Eutaeniichthys cf. gilli was studied here in this respect.
In this study, we report detailed morphological features, including morphometric, meristic, and cephalic sensory papillae of E. cf. gilli from Taiwan. In addition, a preliminary exploration of its hiding tunnel structure and symbiotic species were analyzed and discussed. Finally, the possible dispersal pathway leading to the present biogeographic distribution of E. cf. gilli in Japan, Korea and extending to western Taiwan are discussed in the present study.
Materials and methods
Field sampling and resin casting
A total of 56 individuals of E. cf. gilli were collected from the areas of Shengang (24.168094 oN, 120.457894 oE) and Wangong (23.968126 oN, 120.323173 oE) in western Taiwan (Fig 2). Five lots were collected: 8 individuals on June 1, 2016; 7 individuals on November 3, 2016; 12 individuals on February 22, 2017; 6 individuals on June 13, 2018; and 23 individuals on September 21, 2018. The sites were characterized as mudflat areas dominated by the mud shrimp A. edulis . Samples of goby E. cf. gilli were collected carefully from mud shrimp burrows by using a shovel and hand net (mesh size: 0.5 mm) during low tide period. Eutaeniichthys cf. gilli always appeared at the surface of the pool with turbid underground seawater when digging more than 60 cm deep.
The resin casting method has been applied to study the burrow of mud shrimps [14–16]. Previous researchers found that mud shrimps could be fixed in the resin. Therefore, we used this method to find evidence of the existence of E. cf. gilli in the burrows of mud shrimps. A total of 30 resin casts were obtained from Shengang and Wangong from 2016 to 2018 using polyester resin. The liquid resins were poured into the mud shrimp burrows, and retrieved after 24 hours when they turned into a solid cast model.
Specimens were fixed in 10% formalin for three days, and then transferred to 70% ethanol for long-term preservation. The morphological measurements follow Miller  and the meristic analysis followed Chen and Shao . The terminology of cephalic sensory canals and free neuromast organ (sensory papillae) is based on Sanzo , and Huang et al. . All lengths were standard lengths (SL). Examined specimens were deposited at the Biodiversity Research Museum, Biodiversity Research Center, Academia Sinica, Taipei, Taiwan (ASIZP).
Cultivation in the laboratory
In the laboratory, E. cf. gilli were cultured in fresh seawater with air supply at a photoperiod of 12:12 hour light/dark. Live Artemia franciscana Kellog, 1906 nauplii, copepods, Pseudodiaptomus annandalei Swell 1919 and Apocyclops royi (Lindberg, 1940) were provided twice a day (at 11:00 and 15:00 h). This was according to a suggestion by Dôtu  who reported that E. gilli feeds on small-sized crustaceans and other organic matter. Seawater quality was maintained at salinity ranges of 25.0–30.0 PSU knowing that it is a brackish water species [22, 23]. Seawater temperature was maintained at 24–26°C as this was measured on the mud surface near the mud shrimp burrow on June 1, 2016. Our experience showed that E. cf. gilli can successfully survive for more than one year in the laboratory at these conditions.
Image of the sea surface temperature
The images of the Group for High Resolution Sea Surface Temperature Pilot Project (GHRSST-PP)/ Operational SST and Sea Ice Analysis (OSTIA) were obtained from the Fisheries Research Institute, Council of Agriculture, Taiwan. The images of the sea surface temperature were used for discussing the relationship between water mass transport and the biogeographic distribution of E. cf. gilli.
Historical records of Eutaeniichthys gilli
Eutaeniichthys gilli (Japanese name: Himohaze) inhabits sandy-muddy mud, muddy sediment, and tide pools under stones in estuaries . It is distributed in the northwest Pacific region, in temperate waters. This species has been reported in several areas of the coast and estuaries in Japan [21–22, 25–40]; in the Yellow Sea-Masan ; in the Yellow Sea and Bohai Sea of Korea [42, 43]; and in the Gulf of Chihli (Bohai) , in the Yellow Sea and Bohai Sea of China [45–47].
Morphological traits of the examined specimens
ASIZP0080765, 5 specimens, 26.5–31.2 mm SL; collected from Shengang and Wangong, western Taiwan, June 1, 2016.
Diagnosis. Has a low body and a rather slender head which is relatively small. The upper lip is obviously more prominent than the lower lip. The first dorsal fin rays III, second dorsal fin rays I/16–17, anal fin rays I/11, pectoral fin rays 14–15. Second dorsal fin and anal fin base are long. The body is covered with numerous tiny scales. All scales are tiny and scattered, and scales never touch adjacent scales, and are never arranged in a line. The lateral sides of the body with about 16–18 distinct grayish black blotches, dorsal side with 16–17 cross bars, dorsal side of neck with two longitudinal black stripes, forward extending to the interorbital region. Caudal fin has a distinct longitudinal black stripe, starting from the caudal fin base, extending to the rear end.
Description. Morphometric measurements are given in Table 1. Body low and rather slender, sub-cylindrical anteriorly and compressed posteriorly. Head is moderately small, eye high and large; upper lip obviously more prominent than lower lip; and mouth oblique, corner of mouth extending to a vertical line with the posterior margin of the pupil. Anterior nostril is a short tube and posterior nostril is a round hole. Gill-opening restricted, extending ventrally to a vertical line of one-third of the posterior operculum margin.
Coloration in alive specimens (Fig 1).—Head and body pale yellowish white, body side with about 16–18 distinct grayish black blotches, some blotches are slightly fused. Dorsal side has 16–17 cross bars, starting from neck, and extending to the caudal peduncle. Dorsal side of neck has two longitudinal black stripes, which are extending forward to the interorbital region. Cheek and opercular region has some black spots and bars. The pectoral fin base has some black spots. Dorsal fin, pectoral fin, anal fin, and pelvic fin are without any spot. Caudal fin has a distinct longitudinal black stripe, starting from the caudal fin base, and extending to the rear end.
Head canals.—Head pores absent.
Sensory papillae (Fig 3).—Row a short, about two-thirds of orbit diameter; row b long, about three-fourth of orbit diameter, starting from the vertical of rear margin of the orbit; row c approximately equivalent to orbit diameter. Single cp papilla is located under the last papilla of row c. Row d particularly short, about half of orbit diameter. Opercular papillae with rows ot, oi, and os, rows ot and oi well separated. Row f consists of a pair of single papillae.
ASIZP0080765, 31.2 mm SL. Arrowhead indicate the gill opening, scale bar = 1mm.
Fins.—First dorsal fin rays III, second dorsal fin rays I/16–17 (modally 17), anal fin rays I/11, pectoral fin rays 14–15. The first dorsal fin is small and rounded, fin spine II longest. Second dorsal fin base is long, its rear tip of ray extending to the anterior margin of the procurrent caudal ray when depressed. Anal fin base long, its origin inserts below sixth branched soft ray of second dorsal fin. Pectoral fin is broad and rounded. Pelvic fin disc rounded. Caudal fin fan-shaped, the rear edge rounded.
Scales.—Body covered with numerous tiny scales, all scales tiny and scattered, scales never touch adjacent scale, and is never arranged in a line. Predorsal area and belly also covered with tiny scales. Operculum, preoperculum, prepelvic areas, and pectoral fin base are always naked.
Resin casts of burrows trapping symbiotic species
A total of thirty resin cast burrows of the mud shrimp A. edulis were fixed and examined in this study. Among all resin cast burrows, Eutaeniichthys cf. gilli and snapping shrimp (unidentified) were recorded from 3 (10%) and 13 (43.3%) burrows, respectively (Fig 4). Eutaeniichthys cf. gilli were only recorded in burrows between 50 and 80 cm in length, with only one individual in a burrow, whereas the snapping shrimp recorded in all depth of resin burrows. Two classic examples of burrows were shown in Fig 5. Eutaeniichthys cf. gilli were recorded at places near the tunnel mouth (Fig 5A) and at midlength of depth (Fig 5B) in mud shrimp burrows.
Distribution and habitat in Taiwan
So far, the present report is the first record of this species from sandy and muddy bottoms of the intertidal zone in Shengang and Wangong, western Taiwan. There is no observation that E. gilli can dig the inhabited burrow themselves. Instead, the mud shrimp A. edulis is known to construct a strong burrow structure that can change the composition of the sediment . We conclude, therefore, that E. cf. gilli makes use of the burrow as a sheltered habitat that was built by the mud shrimp, A. edulis.
The present study identified E. cf. gilli inhabiting a wetland near the industrial park and hiding in mud shrimp burrows. This species belongs to the family Gobiidae, which is a highly diverse bony fish family worldwide . However, gobiids are important but frequently misidentified and their biology and ecological niche are poorly understood . There are about 247 species from 77 genera in the gobiid family in Taiwan . Historical records of the goby, E. cf. gilli, along the coast of Taiwan are not available. This new record for Taiwan is thus the southernmost record of the species in the world. However, E. gilli in the area of southern Ryukyu is still inadequately known, and our study is the first to document E. cf. gilli in waters of this region. Previous in situ studies found that E. gilli utilized mud shrimp burrows as a breeding habitat during the period from May to August . A laboratory study found that E. gilli spent 25 to 50% of its time in the burrow of the mud shrimp . The burrow of mud shrimp is an important habitat that E. gilli utilized as a nesting site for spawning and deposition of eggs in the shallow tidal creek of Obitsu-gawa River estuary . The E. gilli takes shelter inside the burrows of A. edulis which benefits their reproduction.
All previous studies found that E. gilli is distributes in coastal areas of Kyushu, Japan, the Yellow Sea, and the Bohai Sea of Korea and China. These records of distribution areas were north of 25 degrees northern latitude [21–22, 25–47]. The distribution of E. gilli was related with the Tsushima Current in an earlier report . The distance from the Yellow Sea and Kyushu to the present study area is about 1400 to 1500 km. The China Coastal Current (CCC) of the Yellow Sea and the Bohai Sea flows along northern Taiwan into the eastern Taiwan Strait during the period in November to April when the northeast monsoon prevails . The water mass transport of the CCC might be the major contributor to the geographic dispersal and distribution patterns of E. cf. gilli.
Several studies reported that the water masses of the CCC plays an important role in transporting marine creatures to coastal waters of Taiwan, such as copepods , jellyfish [53–55], zooplankton in general , and affects the communities of macroalgae . The calanoid copepod, Calanus sinicus, provides an example that demonstrates that the CCC drives it from the Yellow Sea and the Bohai Sea to the southern East China Sea area, and to Hong Kong . Sea water temperature images provided by GHRSST-PP/OSTIA from mid-November 2017 to mid-April 2018 demonstrated the cold water mass distribution from the Yellow Sea, the Bohai Sea, along South Korea, Japan and extending southward to the Taiwan Strait where the specimens were collected during the present study (Fig 6). Therefore, the present results suggest that the dispersal and biogeographical distribution of E. cf. gilli are caused by the water mass transport of the CCC, which continues towards Hong Kong and Vietnam. The biogeographic distribution of the mud shrimp, A. edulis, extends from north of Taiwan towards south of Vietnam . Therefore, it has to be studied whether the distribution range of E. gilli extends to even Vietnam in the South China Sea.
Previous investigations have shown the utility of burrows of upogebiid and callianassid shrimps by E. gilli [21, 22, 59]. The records show that E. gilli has a symbiotic relationship with the mud shrimp Upogebia major , and with Upogebia yokoyai [33, 34, 59] in Japan. Shrimp burrows are used by several gobies for symbiotic associations . The records found more than 120 gobiid fishes in symbiosis with alpheid shrimps; the shrimps built burrows and share those with gobies as a refuge providing shelter, gobies give feedback on this symbiotic relationship with a warning signal when predators appear . The mutual association is beneficial to one partner, or both shrimp and goby . The present study is the first to report the associations of E. cf. gilli with the mud shrimp A. edulis burrows worldwide. The records show that the symbiotic hosts of E. gilli can be diverse and are not specific. This association would allow a sympatric coevolution and biogeographic codistribution of goby E. cf. gilli and its host (A. edulis).
The resin casting method has been applied to study the burrow architecture of different mud shrimps, such as Austinogebia edulis , Upogebia major , and Upogebia omissa . In general, resin casting of mud shrimp burrows showed that they were approximately Y-shaped, with an upper U-part [15, 16, 64]. The present study applied this method to catch E. cf. gilli in burrows of the mud shrimp. The results found that the occurrence rate of E. cf. gilli was 10%. The goby prefers burrows with depth longer than 50cm. Observations in the laboratory found that the behavior of E. cf. gilli was very agile. They might easily escape from through the other opening of the burrow. Designing a suitable method to avoid underestimating the number of E. cf. gilli populations and those inhabiting burrows will be a necessary future task.
Only a few studies have measured the lengths of E. gilli. Lengths records range from newly hatched larvae being 3.6 mm  to a maximal length of adults with 50 mm  in the waters of Japan. The values of the lengths among adult E. gilli are similar to those observed in the present study (Table 2). Results of previous reports are insufficient to compare possible geographic differences of lengths in E. gilli. Body lengths measurements of E. cf. gilli larvae could not be done in the present study since larvae were lacking from the samples in the present study. The spawning period of E. cf. gilli in western Taiwan needs to be confirmed by more frequent collections.
Remarkably, all E. cf. gilli examined in this study were collected from mudflat near the Changhua Coastal Industrial Park. This indicates that the species can tolerate a rather polluted environment. The population size of E. gilli is critical here since it affects the endangered status of the species as it is indicated in the Red List in Japan , as threatened and near-threatened goby species [34, 35, 37], rare species  and vulnerable species in the tidal estuarine systems . Currently, there are 102 fish species reported in the endangered list in Taiwan . The present report is the first record of this species in mudflats in western Taiwan. Biological and ecological investigations need more follow-up support. Proven habitat need to consider the interspecific differences in environmental factor preference and tolerance of gobies inhabiting tidal flats . The protection of suitable habitats will be an important biodiversity conservation measure. Since mudflats are often threatened by landfilling, drilling for fossil energy sources in rivers, and sedimentary habitat changes due to changing tidal and riverine currents, they are particularly vulnerable and need a sustainable conservation management measure.
This investigation documents a unique and rare symbiotic goby E. cf. gilli associated with the mud shrimp A. edulis from the mudflat in western Taiwan. The population size and biogeographic distribution of E. cf. gilli in Taiwan needs to be studied in details in the future to provide its sustainable conservation. Furthermore, previous records demonstrate that E. cf. gilli is distributed in the marine waters of China, Korea, Japan, and the present it report from mudflats of the western Taiwan coast. Studies of the phylogenetic relationships of E. gilli are needed to better understand their population differentiation and geographic distribution.
We are grateful to Dr. Shao-Hung Peng and Mr. Chih-Ming Lin for their assistance during the fieldwork sampling in the mudflats of Shengang and Wangong. We particularly appreciate the contribution of PhD candidate of the Institute of Marine Biology, NTOU, master Pei-Wen Lee who provided copepods; and the staff of the National Museum of Marine Science and Technology master Yun-Hong Tsai who provided brine shrimp Artemia as feed of E. cf. gilli. The authors acknowledge support from Fisheries Research Institute for providing the Sea Surface Temperature images.
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