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Antitumor activity of a novel dual functional podophyllotoxin derivative involved PI3K/AKT/mTOR pathway

  • Yongli Li ,

    Roles Conceptualization, Investigation

    changzhenl@yahoo.com (CL); liyongli@sqmc.edu.cn (YL)

    Affiliation College of Basic Medical Science, Sanquan College of Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Tengfei Huang,

    Roles Investigation

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Yun Fu,

    Roles Investigation

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Tingting Wang,

    Roles Data curation

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Tiesuo Zhao,

    Roles Investigation, Methodology

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Sheng Guo,

    Roles Investigation, Methodology

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Yanjie Sun,

    Roles Validation, Visualization

    Affiliation Experimental Teaching Center of Biology and Basic Medicine, Sanquan College of Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Yun Yang,

    Roles Data curation, Methodology

    Affiliation College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China

  • Changzheng Li

    Roles Conceptualization, Writing – review & editing

    changzhenl@yahoo.com (CL); liyongli@sqmc.edu.cn (YL)

    Affiliations College of Basic Medical Science, Xinxiang Medical University, Xinxiang, Henan, P. R. China, Experimental Teaching Center of Biology and Basic Medicine, Sanquan College of Xinxiang Medical University, Xinxiang, Henan, P. R. China

Antitumor activity of a novel dual functional podophyllotoxin derivative involved PI3K/AKT/mTOR pathway

  • Yongli Li, 
  • Tengfei Huang, 
  • Yun Fu, 
  • Tingting Wang, 
  • Tiesuo Zhao, 
  • Sheng Guo, 
  • Yanjie Sun, 
  • Yun Yang, 
  • Changzheng Li
PLOS
x

Abstract

The progression of cancer through local expansion and metastasis is well recognized, but preventing these characteristic cancer processes is challenging. To this end, a new strategy is required. In this study, we presented a novel dual functional podophyllotoxin derivative, 2-pyridinealdehyde hydrazone dithiocarbamate S-propionate podophyllotoxin ester (PtoxPdp), which inhibited both matrix metalloproteinases and Topoisomerase II. This new podophyllotoxin derivative exhibited significant anti-proliferative, anti-metastatic that correlated with the downregulation of matrix metalloproteinase. In a xenograft animal local expansion model, PtoxPdp was superior to etoposide in tumor repression. A preliminary mechanistic study revealed that PtoxPdp induced apoptosis and autophagy via the PI3K/AKT/mTOR pathway. Furthermore, PtoxPdp could also inhibit epithelial–mesenchymal transition, which was achieved by downregulating both PI3K/AKT/mTOR and NF-κB/Snail pathways. Taken together, our results reveal that PtoxPdp is a promising antitumor drug candidate.

Introduction

Tumor progression through local expansion and metastasis has been well documented. An approach to efficiently inhibit tumor metastasis is urgently required in clinical practice. Numerous strategies have been proposed to address this; although improvements have been achieved in cancer treatment, efficient approaches for different cancers are still lacking and need to be solved. Topoisomerases are highly enriched in rapidly proliferating cells, and play a crucial role in replication, transcription, and chromosome segregation [1,2]. Etoposide as a topoisomerase (Topo) II inhibitor is widely used in clinical practice. Etoposide is a podophyllotoxin (Ptox) derivative isolated from the Podophyllum species [3], but severe side-effects and multidrug resistance (MDR) often result in treatment failure. To overcome the current limitations, numerous approaches have been made to modify the structure of Ptox [4], including esterification and amination at position 4 to produce 4′-demethylepipodophyllotoxin (DMEP) [5], C-S bond modified aromatic heterocyclic podophyllum derivatives [6], acyl thiourea derivatives of epipodophyllotoxin [7], pyridine acid ester derivatives of Ptox [8], halogen-containing aniline Ptox derivatives [9], and combination of 5-Fu with DMEP derivatives [10]. These modifications were achieved using a molecular hybridization strategy [11,12], and some of the derivatives displayed much better cytotoxic activity than etoposide at the cellular level.

It is well-known that tumor metastasis is responsible for approximately 90% of all cancer-related deaths [13]. In the early stages, the metastatic tumor cells secrete signaling molecules to recruit myeloid cells, that later differentiate into tumor associated macrophages, providing chemokines and metalloproteinase to promote both tumor cell growth and degrade the extracellular matrix (ECM) [14]. The soluble components, immunocytes, stromal cells, and ECM comprise the tumor microenvironment, which has an important effect on tumor proliferation and metastasis. In the tumor microenvironment, the metalloproteinases, such as matrix metalloproteinases (MMPs) and Lysyl oxidase, are either zinc or copper containing enzymes, function to degrade the ECM and correlate with distal metastasis [15,16]. In addition, cancer cells have a high demand for iron and copper in order to maintain robust cell proliferation and metastasis, and therefore, depletion of metal ions, or partial inactivation of metallozymes using metal chelator, is one option to inhibit the proliferation and metastasis of cancer cells [17].

In view of the above mentioned features of tumors, both tumor cells and the irrespective microenvironment should be considered in drug design. In the present study, we proposed a new strategy that combined the structural unit of a metal ion chelator with a Topo II inhibitor, in an attempt to achieve the goal of both inhibiting tumor cell growth via Topo II inhibition and inactivating metalloproteinase by chelation, when administered intravenously. To this end, dithiocarbamates were chosen, not only for their strong chelating ability toward metal ions and proven ability to inactivate metalloenzymes [18,19], but also because of their prospective use in cancer therapy [2023]. On the other hand, topoisomerases were chosen since it is well-known that they play important roles in cell proliferation, and that inhibition of topoisomerases, especially topoisomerase II, can significantly suppress tumor growth. It is also well-known that podophyllotoxin (Ptox) is a good structural unit as a topoisomerase II inhibitor. In this study, a novel compound, 2-pyridinealdehyde hydrazone dithiocarbamate S-propionate podophyllotoxin ester (PtoxPdp) was prepared using multi-step reactions based on a hybridization strategy. As expected, the new compound exhibited significant inhibition of both Topo II and MMPs in vitro. A growth inhibition assay against liver cancer cells in vitro revealed that PtoxPdp displayed a better anti-proliferative effect than the parent compounds, 4′-demethylepipodophyllotoxin and etoposide, and similar results were observed in a xenograft model. Moreover, PtoxPdp exhibited a significant anti-metastatic effect and inhibition in a wound healing and invasion assay, which likely correlated with MMP inhibition. In view of the fact that PtoxPdp induced excess ROS within cells, the correlation between ROS and apoptosis, and signaling pathways was further analyzed. The preliminarily data revealed that the antitumor activity of PtoxPdp was manifest through multiple pathways including alterations in apoptosis, autophagy and the PI3K/Akt/mTOR pathway. Interestingly, PtoxPdp also could reverse the epithelial-mesenchymal transition (EMT) in liver cancer cells, which was achieved by downregulation of both the NF-κB/Snail and PI3K/AKT/mTOR pathways; this feature is first reported for an etoposide derivative

Materials and methods

Materials

MTT, ethidium bromide (EB), 2,2’-di-pyridylketone, RPMI-1640, and other chemicals were purchased from Sigma-Aldrich. Fetal bovine serum was purchased from Every Green Zhejiang Tianhang Technology Co. Ltd. (Hang Zhou, China). Anti-NF-κB antibody was obtained from Proteintech Group (Wuhan, China); Antibodies against vimentin, slug, caspase 3, β-actin, Bax, and Bcl-2 were purchased from Boster (Wuhan, China). Antibodies against AKT, p-AKT, mTOR, E-cadherin, and Gapdh were purchased from Enogene (Nanjing, China). 4′-demethylpodophyllotoxin was purchased from Shanghai PureOne Biotechnology (Shanghai, China)

Preparation of pyridinealdehyde hydrazone dithiocarbamate S-propionic acid podophyllotoxin ester (PtoxPdp)

PtoxPdp was synthesized using a four step reaction: the first three-step reaction was similar to a previously reported protocol, except that 2-pyridylaldehyde was used as starting material [24]. Briefly, hydrazine dithiocarbamate (compound I) was synthesized by reacting an equimolar amount of carbon disulfide (1mmol) with hydrazine (1 mmol) in KOH containing ethanol (10 mL) in an ice bath for 1h. Following this, the reaction mixture without further separation was mixed with an equimolar amount of 2-pyridylaldehyde (1 mmol) and the resulting mixture was refluxed for 1h. After cooling, the red-brown solid (compound II) was filtered and washed with cold ethanol. TLC analysis showed one spot. Next, the red-brown (2-pyridylhydrazone dithiocarbamate, 1mmol)) was dissolved in absolute ethanol, and reacted with 3-bromo propionic acid at room temperature for 1h. The yellow solid product (compound III) was filtered and then washed with ethanol. Compound IV (PtoxPdp) was obtained by reacting compound III with 4′-demethylpodophyllotoxin with DCC/DMAP catalysis in absolute CH2Cl2. TLC traced during the period of reaction. 1HNMR (Broker, ppm): NMR: 1HNMR (DMSO-d6): 14.95 (s, 1H), 8.81 (d, 1H, J = 4Hz), 8.57 (m, 1H, J = 4Hz), 8.00 (m, 3H, J = 4, 8Hz), 7.51 (m, 3H, J = 4, 8 Hz), 6.96 (s, 1H), 6.54 (s, 1H), 6.31 (s, 2H), 6.01 (s, 2H), 5.48 (d, 1H, J = 8Hz), 4.75 (dd, 1H, J = 4 Hz), 4.60 (dd, 1H, J = 4Hz), 4.36 (d, 1H, J = 8Hz), 4.20 (dd, 1H, J = 8Hz), 3.61 (s, 6H), 3.30(d, 2H, J = 4Hz), 2.82 (m, 1H, J = 4 Hz). 13CNMR (100 MHz, DMSO-d6): 174.96, 169.61, 162.31, 151.33, 147.67, 147.60, 147.56,147.37, 146.68, 139.39, 137.40, 135.15, 133.85, 133.72, 130.74, 127.24, 125.15, 120.00, 110.38, 110.20, 109.81, 107.86, 101.40, 67.76, 64.74, 56.38, 43.75, 40.48, 38.69, 36.06, 31.08. ESI-MS (microTOF-Q III, Brucker): molecular composition: C31H29N3O9S2; m/z: 674.1239 (M+Na, calculated: 674.1243).

DNA Topo II activity assay

The nuclear extract from HepG2 cell was prepared as previously described [25]. Nuclear extract (0.4 μg) was added to the Topo reaction mixture containing 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 1 mM ATP, 150 mM NaCl, 0.1% BSA (bovine serum albumin), 5% glycerol, and 0.4 μg pUC18 and 1–3 μL of test agent (1 mM PtoxPdp in 8% DMSO) in a final volume of 20 μL. Following incubation at 37°C for 30 min., the reaction was terminated by adding 5 μL of stopping buffer (10% SDS, 0.025% bromophenol blue and 10% glycerol). The reaction products were analyzed by electrophoresis using a 1% agarose gel in a TBE buffer (89 mM Tris-HCl, 89 mM boric acid and 62 mM EDTA) containing 0.1% SDS at 45 V for 3 h. The gel was stained with ethidium bromide (0.5 μg/mL) and photographed using a short wavelength UV lamp on a Tocan 360 gel scanner (Shanghai Tiancheng Technology Inc, China). The assay was performed in duplicate.

Molecular docking

The structure of human type II Top (3QX3) was obtained from RCSB Protein Data Bank. The structure of PtoxPdp was generated by Chemdraw (Chemdraw Ultra 8.0, Cambridge Soft, USA). Energy minimization was conducted using Chem3D (Ultra 8.0, Cambridge Soft, USA) [26]. PyMol and LigPlot were used to display the conformation and interactions [27,28]

Molecular docking studies were performed using AutoDock Vina and AutoDock Tools based on the recommended procedure [29]. The grid box was set to the center of the etoposide model, and the grid box size for PtoxPdp was set to 22, 24, and 28 for the X, Y, and Z axes, respectively. PtoxPdp was set as a flexible ligand by using the default parameters of the AutoDock Tool. The optimal conformation of the ligand was generated using AutoDock Vina.

Migration assay

The inhibition of tumor cell migration by PtoxPdp was determined using a wound-healing migration assay [30]. Briefly, HCCLM3 cells were allowed to grow to full confluence in 24-well plates, after which “wounds” were created using a sterile pipette tip. Following this procedure, the cells were rinsed twice with PBS to remove unattached cells. Fresh medium containing 10% fetal bovine serum and various concentrations of PtoxPdp was then added. The image was photographed and distances between scratches measured under inverted microscopy (×20 magnification).

Invasion assay

As described previously [31], transwell chambers (Corning) with 8-μm-pore membranes coated with Matrigel were used to perform the invasion assay. Briefly, after overnight pretreatment with PtoxPdp in a 6-well plate, the HCCLM3 cells were starved for 12 h in serum-free medium. Following this, the cells were collected and re-suspended as a single cell suspension. In total, 3×104 cells in 100 μL of serum-free medium were added to the upper chamber, and 600 μL of complete medium was added to the lower chamber. Following incubation for 18 h at 37°C, the invading cells were fixed in 4% paraformaldehyde and stained with 0.1% crystal violet and photographed under a microscope (or counted manually). The percentage of inhibition was expressed using control wells as 100%.

Gelatin zymography assay

Gelatin zymography was performed as previously described [32]. Conditioned media were collected from HCCLM3 cells after culture for 24 h in serum free medium with or without PtoxPdp. After collection, the media were centrifuged to pellet any insoluble material. The protein concentration in the conditioned media was quantified using the Bradford method. Equal amounts of conditioned media were mixed with sample buffer and applied to 10% SDS polyacrylamide gel copolymerized with 1 mg/mL of gelatin. After electrophoresis, the gel was incubated in re-naturing buffer (50 mM Tris HCl, pH7.5, 2.5% Triton X-100, 200 mM NaCl, 10 mM CaCl2 and 1 μM ZnCl2), followed by a 24 h incubation with developing buffer (50 mM Tris-base, 200 mM NaCl, 10 mM CaCl2 and 1 μM ZnCl2, 0.02% NaN3). The gel was then stained with 0.25% Coomassie blue R-250 for 1 hour and destained with 10% methanol with 5% acetic acid. Clear bands against a dark blue background indicated where the protease had digested the gelatin and were taken to be indicative of protease activity.

Cytotoxicity assay (MTT assay)

A 10 mM PtoxPdp solution in 80% DMSO was diluted to the required concentration with cell culture medium. The MTT assay was conducted as previously described [25]. Briefly, 5×103/mL HepG2 (or HCCLM3) cells in exponential-phase were seeded at equivalent cell densities into a 96-well plate, and various amounts of PtoxPdp were added to the adhered cells. After 48 h incubation at 37°C in a humidified atmosphere of 5% CO2, 10 μL of MTT solution (5 mg/mL) was added to each well, followed by a further 4 h incubation. The cell culture was removed and 100 μL DMSO was added to each well to dissolve the formazan crystals. The measurement of the solutions absorbance, to give a reading of the number of live cells, was performed on a microplate reader (MK3, Thermo Scientific) at 570 nm. Percent growth inhibition was defined as the percent absorbance inhibition within appropriate absorbance in each cell line. The same assay was performed in triplicate.

Cell clonogenic assay

To mimic individual cell development into macroscopic cell clones, a cell clonogenic assay was performed as previously described [33]. HepG2 cells were seeded into a 6-well plate and allowed to grow for 24 h. Cells were then treated with different concentrations of PtoxPdp for 24 h, after replaced the media, and allowed the cells grow in normal media for 14 d. Next the colonies were fixed in 3.7% paraformaldehyde, stained with 0.1% crystal violet and counted manually.

HepG2 xenograft animal model

A xenograft model was established in BALB/c female nude mice, 4–6 weeks of age and weighing 19–21 g (Beijing Vital River Laboratory Animal Technology Co., Ltd, China). The mice were kept in specific-pathogen-free environments at 26–28°C under 40–60% relative humidity, with a 10-h light/14-h dark cycle, and food and water were provided ad libitum. HepG2 cells (1 × 107 in 0.1 mL PBS) were inoculated into the mammary fat pads of Balb/c nude mice. When tumors were palpable (on day 5, 60 ~ 188 mm3), the 12 male mice were divided randomly into four groups (n = 3 per group), Thereafter, PtoxPdp (normal saline plus DMSO and etoposide) at 0.5 mg/kg, 1 mg/kg was administered via intralesional injection every six days. Tumor growth was measured every two days with a caliper and the volume was calculated according to the formula: V = 1/2 (dlength × d2width) [34]. On day 20, mice were killed via cervical dislocation under deep isoflurane anesthesia (3% for induction, until breathing of the animal was slow) [35]. Each excised tumor was weighed individually and recorded using a digital camera. Half of each primary tumor was snap frozen in liquid nitrogen and stored at -80°C. The other half was fixed and prepared for immunohistochemical analysis. The animal studies were approved by the Animal Welfare and Ethics Group of the Laboratory Animal Science Department, Xinxiang Medical University.

Flow cytometry analysis of apoptosis and cellular ROS

Cellular ROS determination was performed based on previously described [36]. Apoptosis was measured using the Apoptosis Detection Kit (Dojindo Laboratories) as company recommended. Briefly, HepG2 cells were treated with PtoxPdp for 24 h. Following this, cells were collected, washed, and stained with annexin V-FITC and propidium iodide (PI) following the manufacturer’s instruction (Dojindo Laboratories, Japan). The intracellular ROS assay was similar to the above mentioned protocol, except that H2DCF-DA was used to stain the cells.

Comet tail assay

The comet assay tail assay was adapted from the literature, as described [25]. Briefly, HepG2 cells were treated with or without PtoxPdp (0.75 and 1.5 μM) for 24 h. The cells were collected and embedded in 0.5% low melting-point agarose at a final concentration of 104 cells/mL. The cell suspension (20 μL) was then spread onto duplicate frosted slides that had previously been covered with 1% normal melting-point agarose as a basal layer. Slides were allowed to solidify for 10 min at 4°C, then placed in lysis buffer for 1 h (2.5 M NaCl, 0.1 M ethylene diamine tetraacetic acid (EDTA), 0.01 M Tris-HCl,1% Triton X-100, 10% DMSO, pH 10.0). After lysis, the slides were transferred to an alkaline buffer for 40 min (0.001 MEDTA, 0.3 M NaOH, pH >13.0) to allow the DNA to unwind before electrophoresis at 0.66 V/cm and 300 mA for 30 min. All these steps were conducted in the dark. After neutralization in 0.4 M Tris-HCl, pH 7.4, the slides were stained with EB (20 μg/mL) and covered with a cover-slip. Images were then captured using a fluorescent microscopy.

Autophagy and lysosomal membrane permeability affected by PtoxPdp

Cells were seeded into a 24-well plate and treated as described above for the cell viability assay. The cells were treated with different concentrations of PtoxPdp for 24 h. In order to detect the acidic cellular compartment, acridine orange (or LysoTracker Red; Invitrogen) was used, which emits bright red fluorescence in acidic vesicles, but green fluorescence in the cytoplasm and nucleus. After treatment of the cells with PtoxPdp, acridine orange was then added at a final concentration of 1 μg/mL (the recommended concentration of LysoTracker Red) for a period of 15 min. Following PBS washing, fluorescent micrographs were captured using an inverted fluorescence microscope.

Western blotting analysis

Briefly, 1 × 107 HepG2 cells, treated with or without PtoxPdp, were scraped in lysis buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1.0% NP-40, 10% glycerol, and protease inhibitors) and subjected to sonication, followed by centrifugation at 14,000 × g. The clear supernatant was stored at -80°C. Protein concentration was determined using a colorimetric Bio-Rad DC protein assay using the MK3 microplate reader at 570 nm. Proteins (30 μg) were separated on a 13% sodium dodecyl sulfate-polyacrylamide gel at 200 V for 1 h. The separated proteins were subsequently transferred onto a PVDF membrane at 60 V for 1 h. The membrane was washed three times with Tris-buffered saline (TBS) and then blocked for 2 h in TBS containing 0.1% Tween-20 and 5% non-fat skimmed milk. The membrane was incubated at 4°C overnight with the appropriate primary antibody used at a dilution of 1:300 in TBS plus 0.1% Tween-20 (TBST). The membrane was then washed several times with TBST and subsequently incubated with the appropriate HRP-conjugated secondary antibody (1:2,000 in TBST) for 1 h at room temperature. Following washing with TBST, the protein bands were detected using a super sensitive ECL solution (Boster Biological Technology Co. Ltd. China), and visualized using an SYNGENE G:BOX Chemi XX9 (SYNGENE, UK).

Statistical analysis

Results are presented as the mean ± SEM. Comparisons between two groups were carried out using the two-tailed Student’s t-test. Comparisons between multiple groups were performed by one way ANOVA with Dunnett post-hoc correction. All statistical tests were conducted by using IBM SPSS Statistics (version 19 software). P < 0.05 was accepted as significant.

Results

Preparation of 2-pyridinealdehyde hydrazone dithiocarbamate S-propionate podophyllotoxin ester (PtoxPdp)

PtoxPdp was prepared using a four-step reaction (Fig 1), as described in detail in the Materials and Methods section. The first three-step reaction for the preparation of 2-pyridinealdehyde hydrazone dithiocarbamate S-propionate was followed as previously described [24]. The final product, PtoxPdp, was synthesized by mixing 4′-demethylepipodophyllotoxinwith 2-pyridinealdehyde hydrazone dithiocarbamate S-propionate through catalysis with DMAP/DCC in dichloromethane at room temperature. The reaction process was monitored by TLC. Upon completion, the crude product was subjected to flash chromatography and the isolated product had sufficient purity (>98%, S1 Fig). Following NMR and HRMS characterization (S2S4 Figs), PtoxPdp was identified as the expected product.

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Fig 1. Synthetic route used for the preparation of pyridinealdehyde hydrazone dithiocarbamate S-propionic acid podophyllotoxin ester; the individual reaction conditions are shown in the figure.

https://doi.org/10.1371/journal.pone.0215886.g001

Topo II inhibition of PtoxPdp and simulation of binding

Etoposide is a well-known Topo II inhibitor. In order to understand whether the conjugate, PtoxPdp, retained the same activity as etoposide, we first assessed its inhibitory effect on DNA relaxation. Following a previously reported protocol [25], pUC18 plasmid DNA was incubated with a nuclear extract in the absence or presence of various concentrations of PtoxPdp, and the reaction products were subjected to agarose gel electrophoresis. As shown in Fig 2A, PtoxPdp in the presence of ATP also displayed a certain degree of Topo inhibition because the amount of relaxed DNA was decreased compared to the control (Fig 2A). Compared to etoposide, PtoxPdp appeared to be a more potent Topo II inhibitor since higher concentration of etoposide (50 μM) was required to achieve the same level of inhibition observed with PtoxPdp (or about 66 folds decrease for PtoxPdp to achieve similar inhibition of etoposide). We next determined whether the binding of PtoxPdp to Topo II was similar to that of etoposide. To do this, a theoretical simulation was conducted using a molecular docking approach. The human type II Topo crystal structure (PDB ID: 3QX3) was obtained from the RCSB Protein Data Bank. To ensure the accuracy of our docking protocol, etoposide was re-docked into the Topo-DNA complex based on the recommended procedure (S5 Fig), next the PtoxPdp was docked into the Topo II complex (Fig 2B); the simulating affinity energy was -13.6 kcal/mol (compared to that of docked etoposide (-14.8 kcal/mol), a slightly weaker interaction was therefore observed), revealing that replacement of the sugar at the 4-position with the dithiocarbamate derivative did not lead to significant changes in affinity or in the nearby molecular environment of the Topo-bound molecule. The interaction of PtoxPdp with its nearby residues is shown in Fig 2C, clearly showing that the natures of the interactions were mainly Van der Waals forces and hydrogen bonds.

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Fig 2. Topo II inhibition and interaction with PtoxPdp.

(A) Topo II inhibition by PtoxPdp. Line 1, pUC18; line 2, pUC18 plus nuclear extract; line 3, pUC18 plus nuclear extract and etoposide (50 μM); line 4, pUC18 plus nuclear extract and 0.75 μM PtoxPdp; line 5, pUC18 plus nuclear extract and 1.5 μM PtoxPdp. (B) PtoxPdp docked to the Topo II-DNA complex. (C) Interaction between PtoxPdp and nearby residues. CL = cleaved; L = linear; S = supercoiled; R = relaxed DNA. Unk0 = PtoxPdp. Dark green dash lines indicate hydrogen bonds. The eye-like curved dark red lines represent the hydrophobic residues in contact.

https://doi.org/10.1371/journal.pone.0215886.g002

PtoxPdp inhibits liver cancer cell proliferation

In view of excellent Topo II inhibitory activity of PtoxPdp, growth inhibition assay was required to determine whether cytotoxicity was affected by the structural modification. In addition, liver cancer ranks the fifth in malignant tumor relative death, therefore, the effect of PtoxPdp on the proliferation of two liver cancer cell lines was preliminarily investigated. The dose-response curves for the two cell lines are shown in Fig 3A. PtoxPdp displayed significant inhibition of growth in the two examined cell lines following statistical analysis (p<0.01, at 0.78 μM). However, a differential effect on the cell lines was also obvious; growth inhibition was achieved at a lower concentration for HepG2 cells (IC50 = 3.89 ± 0.51 μM for HepG2 cells, Fig 3A1), whereas a much higher concentration was required for HCCLM3 cells (IC50 = 11.01 ± 0.63 μM for LCCM3, respectively, Fig 3A2), indicating that HepG2 cell was more sensitive to PtoxPdp. Interestingly, PtoxPdp exhibited better activity than etoposide in inhibiting the proliferation of these liver cancer cells (S6 Fig). In view of the chelating feature of PtoxPdp, the inhibition of proliferation by PtoxPdp in the presence of copper ions was also investigated. Unexpectedly inhibition of growth was dramatically increased in the two cell lines, compared to that induced by PtoxPdp alone (IC50 = 1.52± 0.20 μM for HepG2 and 2.95 ± 0.40 μM for LCCM3, respectively, Fig 3A), which stemmed from the formation of PtoxPdp-Cu complexes (S6 Fig). The value of IC50 determined the concentration used in subsequent experiments. The dose at half of IC50, or decreasing exposure time to the cells was adopted in order to decrease cytotoxicity of the agent (see migration and invasion assay). Furthermore the ability of HepG2 cells to form colonies, with or without PtoxPdp treatment, was examined for a period of two weeks. As expected, PtoxPdp significantly inhibited colony formation, with ~30% inhibition at 0.75 μM (Fig 3B2), while the survival rate was reduced to ~10% at 1.50 μM treatment (Fig 3B3). The quantitative analysis in alteration of the colony numbers/view in each group was showed in Fig 3B4, clearly there was significant difference between the groups in statistics (p<0.05 for 0.75 μM PtoxPdp, and p< 0.001 for 1.50 μM PtoxPdp).

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Fig 3. Inhibition of growth and colony formation by PtoxPdp.

(A) Growth inhibition by PtoxPdp of the different hepatocellular carcinoma cells lines. The IC50s for PtoxPdp and PtoxPdp-Cu were 3.89 ± 0.51 and 1.52 ± 0.20 μM against HepG2 cells, respectively; and 11.01 ± 0.63 and 2.95 ± 0.40 μM against HCCLM3 cells, respectively. (B) Inhibition of HepG2 cell colony formation by the different indicated concentrations of PtoxPdp; the static data are shown in columns (**P < 0.05; ***P < 0.001).

https://doi.org/10.1371/journal.pone.0215886.g003

PtoxPdp inhibits cell migration and invasion

Both cell invasion and migration are of fundamental importance in tumor metastasis and angiogenesis [37]. To determine the effects of PtoxPdp on the cell invasion, the highly invasion malignant HCCLM3 cell was chosen, and a transwell assay was performed. To minimize the effect of cytotoxicity of PtoxPdp on invasion and migration, the dose was used at 1/14, 1/7 IC50 and shorter exposure time (18 h). As shown in Fig 4A, HCCLM3 cells displayed a high invasion capability (Fig 4A1). In contrast, PtoxPdp significantly attenuated the invasion capacity of these cells in a dose-dependent manner (Fig 4A2 and 4A3); a quantitative analysis is presented in Fig 4B, clearly the numbers of invasive cells into matrix gel were significantly decreased upon exposure of PtoxPdp to the cells compared to that of DMSO (p < 0.001). A wound-healing model is often widely used to estimate the migration potential of endothelial cells. Therefore, the effect of PtoxPdp on the migration of HCCLM3 cells was determined. As shown in Fig 4C, the migration of HCCLM3 cell across the wound space was inhibited by PtoxPdp in a dose-dependent manner, or the healing capacity of the cells was markedly inhibited (in terms in width, p < 0.001) compared DMSO with PtoxPdp (Fig 4D), indicating that PtoxPdp owned certain inhibitory effect on migration and invasion. To understand the potential correlation with MMP inhibition, western blotting and gelatin zymography analysis were conducted. As shown in Fig 4E, treatment with PtoxPdp significantly reduced both MMP-2 and MMP-9 expression and activity, indicating that PtoxPdp displayed significant inhibition of migration and invasion that correlated with MMP repression.

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Fig 4. Inhibition of liver cancer cell migration and invasion by PtoxPdp.

(A) Inhibition of HCCLM3 cells migration by PtoxPdp. (B) Inhibition of HCCLM3 cell invasion by PtoxPdp and quantification. The invasive cells were stained with crystal violet. The results were expressed as invasive cell numbers per field of view (mean± 5 SD, n = 6). ***P < 0.001 compared with DMSO-treated group. (C) Wounded HCCLM3 cells were treated with 0, 0.75, or 1.50 μM PtoxPdp for 18 h. (D) Quantitative statistics analysis of the width of gaps in the wound assay in (C). ***P < 0.001 compared with DMSO-treated group. (E) Western blotting (top) and gelatin zymography (bottom) analyses of matrix metalloprotease inhibition, under the indicated conditions. The object size: 10×10 in Fig 4A and 20×10 in Fig 4C.

https://doi.org/10.1371/journal.pone.0215886.g004

PtoxPdp inhibits HepG2 cell growth in a xenograft cell model

Given that PtoxPdp had such significant biological activity, it was worth evaluating its effects in an animal model. To evaluate the effect of PtoxPdp on tumor growth in vivo, we first established an implantation model by injecting HepG2 cells (5×106) into the flanks of athymic nude mice, and allowed them to develop into a xenograft over a period of two weeks. Once the tumor was palpable, the mice were divided randomly into four experimental treatment groups. Different doses of PtoxPdp (0.5 and 1 mg/kg) were administered intraperitoneally weekly to two groups, one group received etoposide as the positive control, and the remaining group received normal saline plus DMSO as the negative control. The changes in the average tumor volume for each group are plotted in Fig 5A. Clearly, following treatment with PtoxPdp and etoposide, tumor sizes were dramatically decreased compared to animals dosed with normal saline/DMSO. Interestingly, PtoxPdp was superior to etoposide in inhibiting tumor growth (Fig 5A). Since body weight loss is brought about by cachexia, less bodyweight loss is evidence for reduced cachexia [38]. As shown in Fig 5B, PtoxPdp did not lead to body weight loss compared to controls (p < 0.05, 5 measurements for NaCl; 1 measurement for PtoxPdp), and was better in this regard than etoposide. Fig 5C showed that PtoxPdp inhibited growth of the tumor xenografts. The excised tumor weights from the animals were also recorded, as shown in Fig 5D; clearly PtoxPdp reduced tumor weight more effectively than etoposide. The excised tumors weighed 0.700± 0.21 g for the normal saline/DMSO group, 0.61 ± 0.36 g for the etoposide group, whereas those animals treated with PtoxPdp weighed 0.116 ± 0.047g (0.5 mg/kg) and 0.043 ± 0.041g, (1 mg/kg) respectively, statistical analysis in tumor weight revealed that the decrease in tumor weight was significant compared PtoxPdp group with both saline/DMSO group and etoposide group (p<0.05), indicating that PtoxPdp was a potent antitumor drug against liver cancer.

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Fig 5. The effect of PtoxPdp in a HepG2 xenograft animal model.

(A) folds increased in tumor volume were defined as the ratio of means of tumor volume to the initial means of tumor volume in the same group; (B) alteration in means of body weight; there was no significant difference before and after treatment within the groups (p = 0.110~0.735), however significance in change of body weight at the end of the study was observed between NaCl and 1mg/Kg treatment group; (C) comparison of etoposide treated with PtoxPdp in Balb/c nude mice; (D) the excised tumors from different nude mouse in same group, and the group as indicated in the figure. The standard deviation in Fig 5A and 5B was obtained by Excell treatment. ***P < 0.05.

https://doi.org/10.1371/journal.pone.0215886.g005

PtoxPdp induces apoptosis

Generation of reactive oxygen species (ROS) is involved in the mechanism of action of many drugs. To understand whether PtoxPdp has a similar mechanism of action, change in intracellular ROS in cells treated with PtoxPdp was investigated using flow cytometry. As shown in Fig 6A, PtoxPdp indeed induced ROS generation following exposure to cells for 24 h. The effect of PtoxPdp on cellular ROS production after different exposure times was also investigated, interestingly ROS generation was similar at all the time points examined (S7 Fig), indicating that cytotoxic or anti-proliferative effects of PtoxPdp correlated with a persistent ROS insult. It has been demonstrated that the ROS cause oxidative damage to DNA and proteins. To explore the correlation between ROS and DNA fragmentation, a comet tail assay was used to assess DNA damage by PtoxPdp. As shown in Fig 6B, PtoxPdp, in a concentration-dependent manner, caused an increase in the amount of comet tail compared to controls, indicating that it increased DNA damage. ROS generation and DNA fragmentation lead to cell death, either via apoptosis or necrosis, and an increased presence of phosphatidylserine in the outer leaflet of the plasma membrane is a surface change common to many apoptotic cells [39]. Annexin V which has a high affinity for phosphatidylserine can therefore be used to detect phosphatidylserine in the outer leaflet of the plasma membrane. Accordingly, the Annexin V-FITC/propidium iodide (PI) double-staining technique was employed to determine the effect of PtoxPdp on apoptosis. As shown in Fig 6C, when HepG2 cells were exposed to PtoxPdp at the indicated concentrations, early apoptosis was increased by 39~49%, and late apoptosis was increased by 5~7%. It was obvious that PtoxPdp induced a large proportion of HepG2 cell in early apoptosis (p<0.001). To further determine the effect of PtoxPdp on apoptosis, the levels of apoptosis-related proteins were assessed by western blotting. As shown in Fig 6D, PtoxPdp increased the expression of cleaved caspase-3 and bax, while the expression of bcl-2 was decreased, indicating that the antiproliferative action of PtoxPdp involved the apoptosis pathway.

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Fig 6. PtoxPdp induces ROS, DNA fragmentation, and apoptosis in HepG2 cells.

(A) PtoxPdp induces intracellular increases in ROS levels. (B) PtoxPdp induces DNA fragmentation at the indicated concentrations. (C) PtoxPdp induces apoptosis, the data were treated by FlowJo software. (D) Western blotting analysis of apoptosis related proteins. The composite images were generated by using Adobe Photoshop. CL = cleaved.

https://doi.org/10.1371/journal.pone.0215886.g006

Changes in lysosome (autophagosome) membrane permeability (LMP) and autophagy in cells following exposure to PtoxPdp

Bax translocation to lysosomal membranes can affect lysosomal membrane integrity [40]. In view of the increased bax levels seen in the apoptotic process, it is possible that lysosomal membrane integrity might be affected by bax. To test this hypothesis, Lysotracker Red which can accumulate within lysosomes, was employed to assess lysosomal membrane permeability (LMP) [40]. As shown in Fig 7A, the red fluorescence intensities in HepG2 cells were significantly increased in PtoxPdp treated cells compared to control cells, indicating that PtoxPdp affected LMP. Furthermore, changes in LMP may be also a response to autophagy, thus the formation of autophagesome was measured using acridine orange staining. As shown in Fig 7B, red granular fluorescence in the acidic vacuoles was observed in the PtoxPdp treated cells, and this increased in a concentration dependent manner, implying that PtoxPdp induces autophagy. To further examine the involvement of autophagy, the levels of LC3 (microtuble-associated protein light chain 3), an autophagosome marker, were measured by western blotting. As expected, there was an increase in the amount of cleaved LC3-II, and a decrease in uncleaved LC3-I, compared to control cells, indicating that autophagy is involved in the cellular events following exposure to PtoxPdp (Fig 7C).

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Fig 7. PtoxPdp induces lysosomal membrane permeability and autophagy in HepG2 cells.

(A) Lysosomal membrane permeability assessed using LysoTracker Red staining. (B) PtoxPdp induces autophagy. The acidic vacuoles in the autophagosomes were stained using acridine orange. (C) Western blotting analysis of changes in the expression of autophagy-related proteins.

https://doi.org/10.1371/journal.pone.0215886.g007

The antitumor mechanism of PtoxPdp involves downregulation of the Akt/mTOR and NF-κB/Snail pathways, accordingly inhibition of EMT

The PI3K/AKT/mTOR pathway is an intracellular signaling pathway that regulates cell survival and proliferation [41]. Interestingly, tumor cells use the PI3K/Akt/mTOR signaling more than normal cells [42,43]. The fact that PtoxPdp exhibited an anti-proliferative effect both in vitro and in vivo prompted us to investigate its mechanism of action in greater detail. Thus, the expression levels of AKT, phospho-AKT (as a measure of AKT activation) and mTOR were determined by western blotting. As shown in Fig 8A, the phosphorylation levels of AKT were decreased by PtoxPdp treatment and accordingly the expression levels of its downstream target mTOR were also suppressed, indicating that the anti-proliferative of PtoxPdp was through inhibition of the PI3K/AKT/mTOR pathway. A previous study has shown that both mTORC1 and mTORC2 regulate EMT, motility, and metastasis in colorectal cancer [44]; therefore, the downregulation of mTOR induced by PtoxPdp may therefore affect EMT status. In order to explore this aspect, changes in the protein levels of markers of the epithelium (E-cadherin) and mesenchymal cells (vimentin) were assessed. As expected, an upregulation of E-cadherin and a downregulation of vimentin were observed following exposure of HepG2 cells to PtoxPdp, implying that PtoxPdp could inhibit EMT transition (Fig 8B). Furthermore, the levels of NF-κB and slug were also decreased, indicating that NF-κB/slug pathway was also involved in the EMT transition, consistent with previous reports [45,46].

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Fig 8. The regulation of the EMT and AKT signaling pathways by PtoxPdp.

(A) AKT/mTor regulation, the images of p-AKT, mTor and gapdh were from different parts of the same gel under different exposure time; (B) EMT regulation, the images of vimentin, E-cadherin and gapdh were from different parts of the same gel under different exposure time. The amount of proteins in different gels were loaded in each line was same. Vim = vimentin; E-cad = E-cadherin. The composite images were generated by using Adobe Photoshop.

https://doi.org/10.1371/journal.pone.0215886.g008

Discussion

Topoisomerases are ubiquitous enzymes that control DNA supercoiling and entanglement, and play an important role in cell growth. Because cancer cells grow faster than normal cells, the strategy of topoisomerase inhibition has been widely used in cancer therapy, despite its lack of selectivity. Etoposide (ETO), as a Topo II inhibitor is well-known, however its moderate efficacy, the development of drug resistance, and its toxic effects have limited its wide use in the clinic [46]. Therefore, new topoisomerases inhibitors that have lower toxicity are still required. In view of Topo II inhibition by DMEP, a number of structural modifications at position 4 in DMEP have been conducted, including esterification and amination [5,810,12]). For esterification, the carboxyl group was linked to either aromatic rings or non-aromatic rings, and an improved anti-proliferative effect was achieved compared to that of DMEP or ETO at the cell level [5]. It should be noted that although many modified derivatives of DMEP have been prepared and structure-activity relationships (SAR) have been explored, there is still a lack of a clear SAR that can guide drug design; therefore, more effort is needed to improve the activity and selectivity of Topo II inhibitors.

Cancer cells reside in specific tissues, alongside concomitant stroma cells, the ECM and other components of the ECM (referred to as the microenvironment) and these can have a profound effect on cancer development and metastasis [47]. Among associated cells, cancer associated macrophages, as scavenger of senile or necrotic cells, can provide enriched iron, cytokines, and proteinases for use by other cells. Given the presence of enriched transition metals and proteinases in the tumor microenvironment, metal chelators have been shown to disturb metal ion homeostasis, as well as metalloproteinase activity, resulting in a delay in the cell cycle or inhibition of metastasis. In the present study, we propose a novel strategy of targeting Topo II in the host cell, and metalloenzymes in the tumor microenvironment, using intravenous administration. To this end, a metal chelator, a dithiocarbamate unit, was introduced as a structural modification of DMEP to achieve these dual functions (Fig 1). An in vitro inhibition experiment revealed that PtoxPdp was as an effective Topo II inhibitor as etoposide. Furthermore, a theoretical simulation further supported these in vitro data, since PtoxPdp was found to bind to the DNA-Topo complex in a similar manner and with similar affinity as etoposide (Fig 2). Gelatinzymography indicated that PtoxPdp could also inhibit the activities of MMPs (Fig 4E). Approximately 90% of all cancer-related deaths arise as a result of metastasis. In the present study, the data from the wound scratch and transwell invasion assays demonstrated that PtoxPdp would be expected to significantly inhibit metastasis. And MMPs are traditionally associated with matrix remodeling, and in particular with cancer invasion. The downregulation of MMP-2, and MMP-9 from western blotting hinted that its anti-metastatic effect of PtoxPdp may correlate with MMP inhibition. Previous studies have revealed that many metal chelators exhibit significant MMP inhibition [48,49], and dithiocarbamates, as NF-κB inhibitors, have been shown to regulate MMP expression in several different cell lines [50,51]. However, PtoxPdp as a dithiocarbamate derivative repressed MMP-2, and MMP-9 expression in the liver cancer cell line was first observed (Fig 4E), this indicated that the structural unit of podophyllotoxin introduced to dithiocarbamate did not alter action pattern in regulation of MMPs. Taken together, these data strongly suggest that our design concept was realized.

Next, we evaluated inhibition of cell proliferation and colony formation by PtoxPdp (Fig 3), revealing that PtoxPdp owned significant antitumor activity. This has also been observed for other podophyllotoxin derivatives [410]. Since PtoxPdp owned excellent antitumor activity against liver cancer in vitro, whether similar activity in vivo required to be determined. Interestingly PtoxPdp was able to suppress xenograft tumor of HepG2 cell and superior to etoposide (Fig 5). This was an exciting result, which may be partly due to better Topo II inhibitory activity than etoposide. The experimental data both from in vitro and in vivo supported that PtoxPdp was a potent candidate of antitumor drug. In view of excellent anti-proliferative effect of PtoxPdp, the underlying mechanism required to be further explored. It is well-known that many chemotherapeutic agents inhibit cell proliferation by generating excess ROS [52]. We anticipated that PtoxPdp might act as a chemotherapeutic agent, such as etoposide [53]. To this end, the ROS level in HepG2 cell was examined following exposure of the cells to PtoxPdp for different periods of time. As expected, PtoxPdp indeed increased ROS generation (Fig 6A) that mediated DNA fragmentation and apoptosis [54,55], thus the DNA fragmentation was determined further using a comet tail assay [56]. As showed in Fig 6, PtoxPdp treatment led to both DNA fragmentation (Fig 6B), and apoptosis. Additional evidence from both flow cytometry and Western blotting analysis further supported the involvement of apoptosis in the mechanism of action of PtoxPdp [57] (Fig 6D). Increased ROS levels can lead to an autophagic response; therefore, we assessed the effect of PtoxPdp on LMP because the upregulated bax may be transferred to lysosomal membranes [40]. As expected, changes in LMP were observed, implying that lysosomal cell death might also be involved in the mechanism of action of PtoxPdp. As previously mentioned, ROS production can induce autophagy and this can be easily measured in cells by monitoring an increase in the levels of LC3-II. In the present study, an increase in LC3-II levels suggested that the anti-proliferative effect of PtoxPdp may also involve autophagy (Fig 7) [41]. However, the question whether the autophagy was due to changes in LMP or due to a cytotoxic effect induced by PtoxPdp will need to be clarified in future studies.

To understand profoundly the mechanism of action of PtoxPdp, the engagement of potent signal pathway was further investigated. It is known that the PI3K/Akt/mTOR pathway plays a critical role in the proliferation, apoptosis, and metastasis of tumor development [58,59], the PtoxPdp might be through similar pathway. As expected, our data indicated that PtoxPdp can significantly downregulate phosphorylated AKT and mTOR (Fig 8), suggesting that the induction of apoptosis caused by PtoxPdp was mediated through the PI3K/AKT/mTOR pathway [60], which was consistent with that reported previously for other podophyllotxin derivative [61]. The epithelial-mesenchymal transition (EMT) is a physiological process, aberrant EMT activation contributes to cancer progression and metastasis [62,63]. Recent report showed that etoposide treatment could reverse TGF-β induced EMT [64]. As an etoposide analog, PtoxPdp would be expected to behave in a similar fashion with respect to the EMT, thus the effect of PtoxPdp on EMT was also investigated, as expected PtoxPdp could upregulate E-cadherin and downregulate vimentin (Fig 8A), suggesting that PtoxPdp had similar ability in inhibition of EMT, which correlated with downregulation of NF-κB and snail1, in accordance with results reported previously [6568]. However, The EMT inhibition induced by PtoxPdp requires to be explored systematically in deep via observation of the change in morphology, immunofluorescence analysis in the presence or absence of TGF-β1, and the signal pathway engaged in the absence or presence of the inhibitor in the future project. In addition, whether the growth inhibition of PtoxPdp in vivo was achieved through influencing phenotype of tumor associated macrophagy (TAM) requires to be further determined [69]. Taken together these data indicated that the PtoxPdp exhibited antitumor activity via multiple signaling pathways.

Conclusions

In conclusion, PtoxPdp exhibited a dual functions both inhibiting Topo II and MMPs. It could inhibit growth of liver cancer cells both in vitro and in vivo. Mechanistically, the action of PtoxPdp involved apoptosis and PI3K/AKT/mTOR pathway. Furthermore, PtoxPdp also inhibited EMT, which may be achieved by downregulating the NF-κB/Snail. Taken together, our findings indicate that PtoxPdp is a promising antitumor drug for potent use in chemotherapy. However, extensive investigations, both in vitro and in vivo, are required in future studies.

The characterization of PtoxPdp, its copper chelating ability and ROS generation were compiled in supporting information.

Supporting information

S1 Fig. Purity of PtoxPdp was determined by HPLC.

https://doi.org/10.1371/journal.pone.0215886.s001

(TIF)

S5 Fig. Molecular simulation of etoposide and PtoxPdp.

(a) Comparison of docked etoposide with crystalized etoposide in DNA-topoisomerase complex; (b) Comparison of docked etoposide with docked PtoxPdp in DNA-topoisomerase complex.

https://doi.org/10.1371/journal.pone.0215886.s005

(TIF)

S6 Fig. The podophyllotoxin and etoposide induced growth inhibition of HepG2 cells.

https://doi.org/10.1371/journal.pone.0215886.s006

(TIF)

S7 Fig.

The interaction of PtoxPdp with copper ion: (a) spectral changes of PtoxPdp when addition of CuCl2 in acetonitrile; (b) ratio of PtoxPdp/Cu was determined based on spectral change; (c) the color change when PtoxPdp mixed with copper ion in aqueous solution.

https://doi.org/10.1371/journal.pone.0215886.s007

(TIF)

S8 Fig.

PtoxPdp induced ROS production at different time period: (a) 6h; (b) 12h; (c) 24h. DMSO group (A1, B1 and C1), 1.56 μM PtoxPdp (A2,B2, and C2); 3.12 μM (A3, B3 and C3).

https://doi.org/10.1371/journal.pone.0215886.s008

(TIF)

References

  1. 1. Leppard JB, Champoux JJ. Human DNA topoisomerase I: relaxation, roles, and damage control. Chromosoma 2005; 114: 75–85. pmid:15830206
  2. 2. Schoeffler AJ, Berge JM. DNA topoisomerases: harnessing and constraining energy to govern chromosome topology. Q Rev Biophys 2008; 41: 41–101. pmid:18755053
  3. 3. Kamal A, Hussaini SMA, Rahim A, Riyaz S. Podophyllotoxin derivatives: A patent review (2012–2014). Expert Opin Ther Pat 2015; 25: 1025–1034. pmid:26027947
  4. 4. Castro MA, Miguel del Corral JM, Gordaliza M, Gómez-Zurita MA, García PA, Feliciano ASan. Chemoinduction of cytotoxic selectivity in Podophyllotoxin-related lignans. Phytochem Rev 2003; 2: 219–233.
  5. 5. Xiao L, Zhao W, Li HM, Wan DJ, Li DS, Chen T, et al. Design and synthesis of the novel DNA topoisomerase II inhibitors: Esterification and amination substituted 4’-demethylepipodophyllotoxin derivates exhibiting anti-tumor activity by activating ATM/ATR signaling pathways. Eur J Med Chem 2014; 80: 267–277. pmid:24793877
  6. 6. Li JL, Zhao W, Zhou C, Zhang YX, Li HM, Tang YL, et al. Comparison of carbon-sulfur and carbon-amine bond in therapeutic drug: 4β-S-aromatic heterocyclic podophyllum derivatives display antitumor activity. Sci Rep 2015; 5: 14814. pmid:26443888
  7. 7. Li WQ, Wang XL, Qian KD, Liu YQ, Wang CY, Yang L, et al. Design, synthesis and potent cytotoxic activity of novel podophyllotoxin derivatives. Bioorgan Med Chem 2013; 21: 2363–2369.
  8. 8. Liu YQ, Yang L, Tian X. Design, synthesis, and biological evaluation of novel pyridine acid esters of podophyllotoxin and esters of 4’-demethylepipodophyllotoxin. Med Chem Res 2007; 16: 319–330.
  9. 9. Zhao W, Yang Y, Zhang YX, Zhou C, Li HM, Tang YL, et al. Fluoride-containing podophyllum derivatives exhibit antitumor activities through enhancing mitochondrial apoptosis pathway by increasing the expression of caspase-9 in HeLa cells. Sci Rep 2015; 5: 17175. pmid:26608216
  10. 10. Chen SW, Tian X, Tu YQ. Synthesis and cytotoxic activity of novel derivatives of 4’-demethylepipodophyllotoxin. Bioorgan Med Chem Lett 2004; 14: 5063–5066.
  11. 11. Meunier B. Hybrid molecules with a dual mode of action: Dream or reality? Acc Chem Res 2008; 41: 69–77. pmid:17665872
  12. 12. Zhang L, Zhang Z, Chen F, Chen Y, Lin Y, Wang J. Aromatic heterocyclic esters of podophyllotoxin exert anti-MDR activity in human leukemia K562/ADR cells via ROS/MAPK signaling pathways. Eur J Med Chem 2016; 123: 226–235. pmid:27484511
  13. 13. Spano D, Zollo M. Tumor microenvironment: A main actor in the metastasis process. Clin Exp Metastasis 2012; 29: 381–395. pmid:22322279
  14. 14. Hiratsuka S, Watanabe A, Aburatani H, Maru Y. Tumourmediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis. Nat Cell Biol 2006; 8: 1369–1375. pmid:17128264
  15. 15. Shay G, Lynch CC, Fingleton B. Moving targets: Emerging roles for MMPs in cancer progression and metastasis. Matrix Biol 2015; 44–46: 200–206. pmid:25652204
  16. 16. Erler JT, Bennewith KL, Nicolau M, Dornhöfer N, Kong C, Le QT, et al. Lysyl oxidase is essential for hypoxia-induced metastasis. Nature 2006; 440: 1222–1226. pmid:16642001
  17. 17. Bedford MR, Ford SJ, Horniblow RD, Iqbal TH, Tselepis C. Iron chelation in the treatment of cancer: a new role for deferasirox?. J Clin Pharmacol 2013; 53: 885–891. pmid:23740857
  18. 18. Orrenius S, Nobel CSI, van den Dobbelsteen DJ, Burkitt MJ, Slater AF. Dithiocarbamates and the redox regulation of cell death. Biochem Soc Trans 1996; 24: 1032–1038. pmid:8968507
  19. 19. Buac D, Schmitt S, Ventro G, Kona FR, Dou QP. Dithiocarbamate-based coordination compounds as potent proteasome inhibitors in human cancer cells. Mini Rev Med Chem 2012; 12: 1193–1201. pmid:22931591
  20. 20. Mansouri-Torshizi H, Saeidifar M, Khosravi F, Divsalar A, Saboury AA, Hassani F. DNA binding and antitumor activity of α-diimineplatinum(II) and palladium(II) dithiocarbamate complexes. Bioinorg Chem Appl 2011; 2011: 394506. pmid:22110410
  21. 21. Milacic V, Chen D, Ronconi L, Landis-Piwowar KR, Fregona D, Dou QP. A novel anticancer gold(III) dithiocarbamate compound inhibits the activity of a purified 20S proteasome and 26S proteasome in human breast cancer cell cultures and xenografts. Cancer Res 2006; 66: 10478–86. pmid:17079469
  22. 22. Nardon C, Schmitt SM, Yang H, Zuo J, Fregona D, Dou QP. Gold(III)-dithiocarbamato peptidomimetics in the forefront of the targeted anticancer therapy: preclinical studies against human breast neoplasia. PLoS One 2014; 9: 84248.
  23. 23. Cattaruzza L, Fregona D, Mongiat M, Ronconi L, Fassina A, Colombatti A, et al. Antitumor activity of gold(III)-dithiocarbamato derivatives on prostate cancer cells and xenografts. Int J Cancer 2011; 128: 206–215. pmid:20209498
  24. 24. Wang TT, Fu Y, Huang TF, Liu YX, Wu MH, Yuan Y, et al. Copper ion attenuated the antiproliferative activity of di-2-pyridylhydrazone dithiocarbamate derivative; however, there was a lack of correlation between ROS generation and antiproliferative activity. Molecules 2016; 21: 1088.
  25. 25. Fu Y, Yang YL, Zhou SF, Liu Y, Yuan Y, Li SS, et al. Ciprofloxacin containing Mannich base and its copper complex induce antitumor activity via different mechanism of action. Int J Oncol 2014; 45: 2092–2100. pmid:25174498
  26. 26. Ok K, Jung YW, Jee JG. Facile docking and scoring studies of carborane ligands with estrogen receptor. Bull Korean Chem Soc 2013; 34: 1051–1054.
  27. 27. DeLano WL. The PyMOL Molecular Graphics System; DeLano Scientific: San Carlos, CA, USA, 2002; Available online: http://www.pymol.org (accessed on 5 July 2012).
  28. 28. Laskowski RA, Swindells MB. LigPlot+: Multiple ligand-protein interaction diagrams for drug discovery. J Chem Inf Model 2011; 51: 2778–2786. pmid:21919503
  29. 29. Trott O, Olson AJ. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J Comput Chem 2010; 31: 455–461. pmid:19499576
  30. 30. Song Y, Dai F, Zhai D, Dong Y, Zhang J, Lu B, et al. Usnic acid inhibits breast tumor angiogenesis and growth by suppressing VEGFR2-mediated AKT and ERK1/2 signaling pathways. Angiogenesis 2012; 15: 421–432. pmid:22669534
  31. 31. Gumireddy K, Li A, Gimotty PA, Klein-Szanto AJ. Showe LC. Katsaros D, et al. KLF17 is a negative regulator of epithelial mesenchymal transition and metastasis in breast cancer. Nat Cell Biol 2009; 11: 1297–1304. pmid:19801974
  32. 32. Pan X, Han H, Wang L, Yang L, Li R, Li Z, et al. Nitidine Chloride inhibits breast cancer cells migration and invasion by suppressing c-Src/FAK associated signaling pathway. Cancer Lett 2011; 313: 181–191. pmid:21959111
  33. 33. Lopez G, Liu J, Ren W, Wei W, Wang S, Lahat G, et al. Combining PCI-24781, a novel histone deacetylase inhibitor, with chemotherapy for the treatment of soft tissue sarcoma. Clin Cancer Res 2009; 15:3472–3483. pmid:19417021
  34. 34. Tomayko MM, Reynolds CP. Determination of subcutaneous tumor size in athymic (nude) mice. Cancer Chemother Pharmacol 1989; 24: 148–154. pmid:2544306
  35. 35. Reynolds CP, Sun BC, DeClerck YA, Moats RA. Assessing growth and response to therapy in murine tumor models. Methods Mol Med 2005; 111: 335–350. pmid:15911989
  36. 36. Huang TF, Li CP, Sun XZ, Zhu Z, Fu Y, Liu YX, et al. The antitumor mechanism of di-2-pyridylketone 2-pyridine carboxylic acid hydrazone and its copper complex in ROS generation and topoisomerase inhibition, and hydrazone involvement in oxygen-catalytic iron mobilization. Int J Oncol 2015; 47: 1854–1862. pmid:26398524
  37. 37. Bergers G, Benjamin LE. Tumorigenesis and the angiogenic switch. Nat Rev Cancer 2003; 3: 401–410. pmid:12778130
  38. 38. Yokoyama M, Okano T, Sakurai Y, Ekimoto H, Shibazaki C, Kataoka K. Toxicity and antitumor activity against solid tumors of micelle-forming polymeric anticancer drug and its extremely long circulation in blood. Cancer Res 1991; 51: 3229–3236. pmid:2039998
  39. 39. Fadok VA, Bratton DL, Frasch SC, Warner ML, Henson PM. The role of phosphatidylserine in recognition of apoptotic cells by phagocytes. Cell Death Differ 1998; 5: 551–562. pmid:10200509
  40. 40. Johansson AC, Appelqvist H, Nilsson C, K?gedal K, Roberg K, Ollinger K. Regulation of apoptosis-associated lysosomal membrane permeabilization. Apoptosis (2010) 15:527–540. pmid:20077016
  41. 41. Fumarola C, Bonelli MA, Petronini PG, Alfieri, RR. Targeting PI3K/AKT/mTOR pathway in non small cell lung cancer. Biochem Pharmacol 2014; 90: 197–207. pmid:24863259
  42. 42. Laplante M, Sabatini DM. mTOR signaling in growth control and disease. Cell 2012; 149: 274–293. pmid:22500797
  43. 43. Strimpakos AS, Karapanagiotou EM, Saif MW, Syrigos KN. The role of mTOR in the management of solid tumors: an overview. Cancer Treat Rev 2009; 35:148–159. pmid:19013721
  44. 44. Gulhati P, Bowen KA, Liu J, Stevens PD, Rychahou PG, Chen M, et al. mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways. Cancer Res 2011; 71: 3246–56. pmid:21430067
  45. 45. Zhang W, Shi X, Peng Y, Wu M, Zhang P, Xie R, et al. HIF-1α promotes epithelial-mesenchymal transition and metastasis through direct regulation of ZEB1 in colorectal cancer. PLoS One 2015; 10:e0129603. pmid:26057751
  46. 46. Huber MA, Azoitei N, Baumann B, Grünert S, Sommer A, Pehamberger H, et al. NF-kappaB is essential for epithelial-mesenchymal transition and metastasis in a model of breast cancer progression. J Clin Invest 2004; 114:569–81. pmid:15314694
  47. 47. Achyut BR, Arbab AS. Myeloid cell signatures in tumor microenvironment predicts therapeutic response in cancer. Onco Targets Ther 2016; 9:1047–1055. pmid:27042097
  48. 48. Huang ST, Yang RC, Wu HT, Wang CN, Pang JH. Zinc-chelation contributes to the anti-angiogenic effect of ellagic acid on inhibiting MMP-2 activity, cell migration and tube formation. PLoS One 2011; 6: e18986. pmid:21573219
  49. 49. Cau SBA, Guimaraes DA, Rizzi E, Ceron CS, Souza LL, Tirapelli CR, et al. Pyrrolidine dithiocarbamate down-regulates vascular matrix metalloproteinases and ameliorates vascular dysfunction and remodelling in renovascular hypertension. Br J Pharmacol 2011; 164: 372–381. pmid:21434884
  50. 50. Kim KS, Oh DH, Choi HM, Bang JS, Ryu CJ, Kim JH, et al. Pyrrolidine dithiocarbamate, a NF-κB inhibitor, upregulates MMP-1 and MMP-13 in IL-1β-stimulated rheumatoid arthritis fibroblast-like synoviocytes. Eur J Pharmacol 2009; 613: 167–175. pmid:19379726
  51. 51. Cau SB, Guimaraes DA, Rizzi E, Ceron CS, Gerlach RF, Tanus-Santos JE. The nuclear factor kappaB inhibitor pyrrolidine dithiocarbamate prevents cardiac remodelling and matrix metalloproteinase-2 up-regulation in renovascular hypertension.Basic Clin Pharmacol Toxicol 2015; 117: 234–241. pmid:25816715
  52. 52. Gorrini C, Harris IS, Mak TW. Modulation of oxidative stress as an anticancer strategy. Nat Rev Drug Discov 2013; 12: 931–947. pmid:24287781
  53. 53. Kurosu T, Fukuda T, Miki T, Miura O. BCL6 overexpression prevents increase in reactive oxygen species and inhibits apoptosis induced by chemotherapeutic reagents in B-cell lymphoma cells, Oncogene 2003; 22: 4459–4468. pmid:12881702
  54. 54. Higuchi M, Honda T, Proske RJ, Yeh ET. Regulation of reactive oxygen species-induced apoptosis and necrosis by caspase 3-like proteases. Oncogene 1998; 17: 2753–2760. pmid:9840939
  55. 55. Solovyan VT, Bezvenyuk ZA, Salminen A, Austin CA, Courtney MJ. The role of topoisomerase II in the excision of DNA loop domains during apoptosis. J Biol Chem 2002; 277: 21458–21467. pmid:11940566
  56. 56. Olive PL, Banáth JP. The comet assay: a method to measure DNA damage in individual cells. Nat Protoc 2006; 1: 23–29. pmid:17406208
  57. 57. Perlman H, Zhang X, Chen MW, et al: An elevated bax/bcl-2 ratio corresponds with the onset of prostate epithelial cell apoptosis. Cell Death Differ 1999; 6: 48–54. pmid:10200547
  58. 58. Shaw RJ, Cantley LCRas. PI(3)K and mTOR signalling controls tumour cell growth. Nature 2006; 441:424–430. pmid:16724053
  59. 59. Ponnurangam S, Standing D, Rangarajan P, Subramaniam D. Tandutinib inhibits the Akt/mTOR signaling pathway to inhibit colon cancer growth. Mol Cancer Ther 2013; 12: 598–609. pmid:23427297
  60. 60. Showkat M, Beigh MA, Andrabi KI. mTOR Signaling in protein translation regulation: implications in cancer genesis and therapeutic interventions. Mol Biol Int 2014; 2014: 686984. pmid:25505994
  61. 61. Zhang L, Wang J, Liu L, Zheng C, Wang Y. Synthesis and antiproliferative activity of novel all-trans-retinoic acid-podophyllotoxin conjugate towards human gastric cancer cells. Molecules 2017; 22: 628. pmid:28420180
  62. 62. Nistico P, Bissell MJ, Radisky DC. Epithelial-Mesenchymal transition: General principles and pathological relevance with special emphasis on the role of matrix metalloproteinases. Cold Spring Harb Perspect Biol 2012; 4: a011908. pmid:22300978
  63. 63. Polyak K, Weinberg RA. Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer 2009; 9: 265–273. pmid:19262571
  64. 64. Aguirre-Alvarado C, Segura-Cabrera A, Velázquez-Quesada I, Hernández-Esquivel MA, García-Pérez CA, Guerrero-Rodríguez SL, et al. Virtual screening-driven repositioning of etoposide as CD44 antagonist in breast cancer cells. Oncotarget 2016; 7: 23772–23784. pmid:27009862
  65. 65. Thiery JP, Sleeman JP. Complex networks orchestrate epithelial-mesenchymal transitions. Nat Rev Mol Cell Biol 2006; 7: 131–142. pmid:16493418
  66. 66. Serrano-Gomez SJ, Maziveyi M, Alahari SK. Regulation of epithelial-mesenchymal transition through epigenetic and posttranslational modifications. Mol Cancer 2016; 15: 18. pmid:26905733
  67. 67. Dong R, Wang Q, He XL, Chu YK, Lu JG, Ma QJ. Role of nuclear factor kappa B and reactive oxygen species in the tumor necrosis factor-alpha-induced epithelial-mesenchymal transition of MCF-7 cells. Braz J Med Biol Res 2007; 40: 1071–1078. pmid:17665043
  68. 68. Zhang K, Zhao J, Liu X, Yan B, Chen D, Gao Y, et al. Activation of NF-κB upregulates Snail and consequent repression of E-cadherin in cholangiocarcinoma cell invasion. Hepatogastroenterology 2011; 58: 1–7. pmid:21510277
  69. 69. Nadella V, Ranjan R, Senthilkumaran B, Qadri S. S. Y. H, Pothani S, Singh A. K, et al. Podophyllotoxin and rutin modulate M1 (iNOS+) macrophages and mitigate lethal radiation (LR) induced inflammatory responses in mice, Front Immunol 2019; 10: 106. eCollection 2019. pmid:30809222