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Normative range of blood biochemical parameters in urban Indian school-going adolescents

  • Khushdeep Bandesh ,

    Contributed equally to this work with: Khushdeep Bandesh, Punam Jha

    Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft

    Affiliations Genomics and Molecular Medicine Unit, CSIR–Institute of Genomics and Integrative Biology, New Delhi, India, Academy of Scientific and Innovative Research, CSIR–Institute of Genomics and Integrative Biology Campus, New Delhi, India

  • Punam Jha ,

    Contributed equally to this work with: Khushdeep Bandesh, Punam Jha

    Roles Data curation, Formal analysis, Methodology, Software

    Affiliations Genomics and Molecular Medicine Unit, CSIR–Institute of Genomics and Integrative Biology, New Delhi, India, Academy of Scientific and Innovative Research, CSIR–Institute of Genomics and Integrative Biology Campus, New Delhi, India

  • Anil K. Giri,

    Roles Validation

    Affiliations Genomics and Molecular Medicine Unit, CSIR–Institute of Genomics and Integrative Biology, New Delhi, India, Academy of Scientific and Innovative Research, CSIR–Institute of Genomics and Integrative Biology Campus, New Delhi, India

  • Raman K. Marwaha,

    Roles Resources

    Affiliation Senior consultant endocrinologist and Scientific Advisor (Projects), International Life Sciences Institute-India, New Delhi, India

  • INDICO ,

    Complete membership of the author group can be found in the Acknowledgments

  • Vinod Scaria ,

    Roles Writing – review & editing (DB); (NT); (VS)

    Affiliations Academy of Scientific and Innovative Research, CSIR–Institute of Genomics and Integrative Biology Campus, New Delhi, India, GN Ramachandran Knowledge Center for Genome Informatics, CSIR–Institute of Genomics and Integrative Biology (CSIR–IGIB), Delhi, India

  • Nikhil Tandon ,

    Roles Resources (DB); (NT); (VS)

    Affiliation Department of Endocrinology and Metabolism, All India Institute of Medical Sciences, New Delhi, India

  • Dwaipayan Bharadwaj

    Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing (DB); (NT); (VS)

    Affiliations Genomics and Molecular Medicine Unit, CSIR–Institute of Genomics and Integrative Biology, New Delhi, India, Academy of Scientific and Innovative Research, CSIR–Institute of Genomics and Integrative Biology Campus, New Delhi, India, Systems Genomics Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India

Normative range of blood biochemical parameters in urban Indian school-going adolescents

  • Khushdeep Bandesh, 
  • Punam Jha, 
  • Anil K. Giri, 
  • Raman K. Marwaha, 
  • INDICO, 
  • Vinod Scaria, 
  • Nikhil Tandon, 
  • Dwaipayan Bharadwaj


Adolescence is the most critical phase of human growth that radically alters physiology of the body and wherein any inconsistency can lead to serious health consequences in adulthood. The timing and pace at which various developmental events occur during adolescence is highly diverse and thus results in a drastic change in blood biochemistry. Monitoring the physiological levels of various biochemical measures in ample number of individuals during adolescence can call up for an early intervention in managing metabolic diseases in adulthood. Today, only a couple of studies in different populations have investigated blood biochemistry in a small number of adolescents however, there is no comprehensive biochemical data available worldwide. In view, we performed a cross-sectional study in a sizeable group of 7,618 Indian adolescents (3,333 boys and 4,285 girls) aged between 11–17 years to inspect the distribution of values of common biochemical parameters that generally prevails during adolescence and we observed that various parameters considerably follow the reported values from different populations being 3.56–6.49mmol/L (fasting glucose), 10.60–199.48pmol/L (insulin), 0.21–3.22nmol/L (C–peptide), 3.85–6.25% (HbA1c), 2.49–5.54mmol/L (total cholesterol), 1.16–3.69mmol/L (LDL), 0.78–1.85mmol/L (HDL), 0.33–2.24mmol/L (triglycerides), 3.56–11.45mmol/L (urea), 130.01–440.15μmol/L (uric acid) and 22.99–74.28μmol/L (creatinine). Barring LDL and triglycerides, all parameters differed significantly between boys and girls (p< 0.001). Highest difference was seen for uric acid (p = 1.3 x10-187) followed by C–peptide (p = 6.6 x10-89). Across all ages during adolescence, glycemic and nitrogen metabolites parameters varied markedly with gender. Amongst lipid parameters, only HDL levels were found to be significantly associated with gender following puberty (p< 0.001). All parameters except urea, differed considerably in obese and lean adolescents (p< 0.0001). The present study asserts that age, sex and BMI are the essential contributors to variability in blood biochemistry during adolescence. Our composite data on common blood biochemical measures will benefit future endeavors to define reference intervals in adolescents especially when the global availability is scarce.


Blood tests are routinely prescribed in healthcare systems to analyze the biochemistry to determine a diseased or a healthy state. A panel of blood tests commonly measures metabolic parameters–glycemic (glucose, insulin), lipids (total cholesterol, LDL, HDL, triglycerides), nitrogen metabolites (urea, creatinine, uric acid); important electrolytes (Na+, K+, Cl-, HCO3-) and crucial enzymes (liver aminotransferases and phosphatases, etc.) [1]. These biochemical parameters vary considerably depending upon an individual's age, sex, ethnicity, dietary intake or physiological state of the body [28].

Age is a major factor that contributes to variation in blood biochemistry [9]. Ageing is a continuous dynamic process that leads to physiological, psychological and behavioral changes resulting in biological aberrations, metabolic dysfunction, cellular senescence or decline in function of organ systems [10, 11]. Amongst various life stages–infancy, childhood, adolescence, adulthood and old age, adolescence is the critical one. It marks the period of transition from childhood to adulthood and is characterized by rapid biological, social and emotional changes [12] that severely impacts future health. This transition is largely governed by gender of an individual that gives rise to difference in hormonal levels among boys and girls at puberty [13]. On account of these multiple significant physiological changes, biochemical parameters tend to vary drastically. Administering the variability of these parameters during adolescence can layoff allied metabolic diseases in adulthood. Currently, a large body of studies have examined variability in blood biochemistry in children in different populations, however only a few inspected adolescents. In view, we analyzed physiological levels of common biochemical parameters in blood (glucose, insulin, C–peptide, HbA1c, total cholesterol {TC}, LDL, HDL, triglycerides, urea, creatinine and uric acid) and studied their variation with Body Mass Index (BMI) in several adolescents (N = 7,618), a number that has never been studied previously. The samples were collected as a part of another ongoing project beyond the intentions to define reference intervals. Therefore, we conveniently aimed to look at the mere dispersion of values of different parameters that normally exists during adolescence.

The physiological changes during adolescence always occur in a universal order however, their timing and pace vary depending upon environmental influences (e.g. food habits, lifestyle, and cultural systems) [1417]. Today, several populations worldwide have encountered rapid urbanization swiftly changing the lifestyle of its people and hence predisposing them to several complex diseases. These changes together with the genetic makeup determine population specific normal range of values for common biochemical parameters. One such population that exhibits huge diversity on grounds of both genetic and lifestyle ways is Indians. Indians amounting to one-sixth of world's total population [18], comprise several endogamous groups that are genetically distinct and highly diverse in terms of dietary intake and ways of living [19]. Indian diets are typically carbohydrate rich compared to the dietary patterns favored in rest of the world [20, 21], and indeed pose Indians at a high risk for metabolic diseases [2124]. Therefore, we exclusively studied Indians at a younger age to institute an early intervention and management of highly prevalent metabolic diseases in the adult population.

Materials and methods

The study was carried out in accordance with the principles of the Helsinki Declaration and approved by Human Ethics Committee of All India Institute of Medical Sciences, New Delhi, India and CSIR–Institute of Genomics and Integrative Biology, New Delhi, India. A written informed consent was obtained from parents/guardians of each participant followed by a verbal consent from participants themselves.

Study population

We conducted the study at CSIR–Institute of Genomics and Integrative Biology, New Delhi, India. A total of 7,618 adolescents (3,333 boys and 4,285 girls) aged between 11–17 years were enrolled from various schools located in urban areas of Delhi–National Capital Region as a part of a study on childhood obesity conducted previously in our laboratory [25]. Average age in total sample group was observed to 13.73 years (± 1.80 years), 13.68 ±1.78 years in boys and 13.77 ±1.77 years in girls. Residing in north part of Indian subcontinent, all participants comprised Indo–European ethnicity and were selected through a pre–designed questionnaire assessing their age, dietary habits, state of birth, parent's state of origin and, past and present medical history. Participants who suffered from any chronic ailments, under medication, history of hospitalization in last six months and self–denial for blood withdrawal were excluded from the study.

The samples were categorized as Obese (OB), Overweight (OW) and Lean or Normal weight (NW) by calculating BMI z-scores and inspecting the previously established BMI percentiles for specific age and sex in children during adolescence (CDC Growth Charts) [26, 27]. BMI at or above 95th percentile at same age and sex was counted as Obese [26]. BMI below 95th percentile but at or above 85th percentile was considered Overweight and BMI in-between 85th percentile and 5th percentile was defined as Lean [26].

Sample collection and biochemical analysis

Participants were subjected to an overnight fast and their peripheral blood was collected by trained phlebotomists in three different vacutainers–serum separator tubes, sodium fluoride vacuette and potassium EDTA tubes manufactured by Becton Dickinson, New Jersey, USA. Samples were transported to laboratory over ice for processing within 2 hours of collection. Different biochemical parameters were measured using well-recognized robust analyzers–COBAS Integra 400 plus and COBAS e411. Details regarding assay methods for studied clinical parameters have been described previously [28].

Statistical analyses

As mentioned, we wanted to capture the natural spread of values for each parameter during adolescence, so no outliers were deleted. Thus, instead of presenting the mean values that are normally affected by the outliers, we rather looked at the median values and interquartile ranges (2.5 percentile and 97.5 percentile). Mann–Whitney U test was used for comparing difference among different groups that were analyzed. P value less than 0.05 was considered significant. R version 3.4.0 was used for statistical analysis ( Although there were a small number of tests (11 parameters) that were performed between different groups, the observed differences were further reviewed after applying Bonferroni correction wherein the corrected p value was assumed to be 0.0045 (α/number of comparisons i.e. 0.05/{11}).

Power calculation

The power of the study was calculated using two different tools separately–OpenEpi, an open-source tool for computing epidemiologic statistics for public health [29] and G*Power 3, a robust power analysis program for the social, behavioral, and biomedical sciences [30]. Power of a two-sided U test of each parameter was computed at α level of 0.05 and 95% confidence intervals from the respective effect size in boys and girls (i.e. = {difference in group means}/{standard deviation}) (S1 Table).


For nearly all studied biochemical parameters, the power of the study was observed to be 100 percent (97 percent for TC) to detect genuine differences between various groups (S1 Table). For LDL and TG, overall comparison among all adolescent boys and girls lacked sufficient power, however their individual comparisons at specific age groups secured necessary power.

Glycemic parameters are strongly associated with gender and adolescence age

Normative range of plasma glycemic parameters in Indian adolescents was 3.56–6.49mmol/l (fasting glucose), 10.60–199.48pmol/l (insulin), 0.21–3.22nmol/l (C–peptide) and 3.85–6.25% (HbA1c) (Table 1).

Table 1. Normative range of common biochemical parameters in Indian adolescents.

Plasma glucose.

Boys consistently showed significantly higher levels of fasting plasma glucose than girls across all ages during adolescence (p ≤ 0.001) (Table 2, S2 Table). Mean value of plasma glucose was observed to be 4.91mmol/L in boys and 4.72mmol/L in girls (p = 6.2 x 10−31) (Table 1). Between subsequent ages, both boys and girls show consistent plasma glucose levels, except for age of 14 years, where plasma glucose levels in boys were observed to decline significantly than other ages during adolescence (Table 2).

Table 2. Variation of glycemic parameters with gender across different ages during adolescence.

Plasma insulin.

Fasting plasma insulin levels were remarkably higher in girls than boys (p = 5.1 x 10−15) (Table 1). This observation prevailed over the ages from 11 years to 14 years (p ≤ 10−4), but at later ages of adolescence both boys and girls featured comparable plasma insulin levels (Table 2). Highest insulin levels were observed to be 65.53pmol/L (median value) at age of 12 years in girls and 60.14pmol/L in boys at age of 16 years (Table 2). Among boys, we noticed that plasma insulin levels change negligibly with age (Table 2, S3 Table). However, in girls, plasma insulin levels change significantly between ages of 12 to 13 years (p = 0.01) and 16 to 17 years (p = 0.008) (Table 2, S4 Table).


Alike plasma insulin, significantly higher levels of C–peptide were found in girls than boys across different ages in adolescence (p ≤ 10−7{11–16 years}, p = 0.046 {16–17 years}) (Table 2). Mean C–peptide levels were 1.43nmol/L in girls in contrast to 1.03nmol/L in boys (p = 6.6 x 10−89) (Table 1). Furthermore, C–peptide levels varied significantly between ages of 11–12 years in both genders–boys (p = 4.73 x 10−4) and girls (p = 1.88 x 10−4) (S3 and S4 Tables). Additionally, boys showed markedly different C–peptide levels between 16 and 17 years of age (p = 0.002) (Table 2).


Mean levels of HbA1c were noted to be 5.24% in girls and 5.08% in boys (p = 3.0 x 10−17) (Table 1). With increasing age in adolescence, girls showed unusually higher HbA1c levels than boys (p < 10−5 {11–13 years}, p = 0.01{15–16 years}) (Table 1). Both boys and girls exhibited significantly different HbA1c levels between ages of 11 and 12 years (p = 0.02 and 0.003) (S3 and S4 Tables). Additionally, girls aged 14 years displayed drastically lower HbA1c levels than their younger mates (p = 0.003) (Table 2).

Amongst blood lipids only HDL varies robustly with gender and age throughout adolescence

In Indian adolescents, plasma levels for different lipids were commonly seen in the range of 2.49–5.54mmol/L (TC), 1.16–3.69mmol/L (LDL), 0.78–1.85mmol/L (HDL) and 0.33–2.24mmol/L (triglycerides) (Table 1). Indian girls had significantly higher TC and HDL levels than boys (respective p = 1.3 x 10−4 and 5.3 x 10−11) (Table 1).

Total Cholesterol.

Across all ages during adolescence, boys and girls revealed identical plasma TC levels except at age of 14 years, when girls have robustly higher levels than boys (p = 2.44 x 10−4) (Table 3). Barring the age of 14 years in boys, no significant change in levels of plasma TC was observed with increasing age during adolescence within each gender (S3 and S4 Tables).

Table 3. Changes in lipid profile in adolescent boys and girls with age.


Like TC, LDL levels were similar in boys and girls across different adolescence ages (Table 3). Besides, no difference was seen with increasing age in either gender (Table 3).


Indian boys and girls portrayed significantly distinct HDL levels from ages 14 years to 17 years (p < 0.001) (Table 3), with girls having higher HDL levels than boys. HDL levels were found to be constant over adolescence period in both the genders (Table 3, S3 and S4 Tables).


Girls showed robustly higher triglyceride levels than boys at ages 11 and 12 years (p = 8.5 x 10−4 and p = 0.01), but unexpectedly lower triglyceride levels at ages 15, 16 and 17 years (p < 0.01) (Table 3, S2 Table). Within boys, triglyceride levels varied significantly between 14 and 15 years (Table 3, S3 and S4 Tables), conversely at 11–12 years among girls (Table 3, S3 and S4 Tables).

Nitrogen metabolites show marked difference among adolescent boys and girls

Plasma nitrogen metabolites were observed to be normally in the ranges of 3.56–11.45mmol/L (urea), 130.01–440.15μmol/L (uric acid) and 22.99–74.28μmol/L (creatinine) in Indian adolescents (Table 1). Boys retained strikingly higher levels of all studied nitrogen metabolites than girls (p = 1.0 x 10−39 {urea}; 1.3 x 10−187 {uric acid} and 1.97 x 10−28 {creatinine}).


Throughout adolescence (11–17 years), girls showed dramatically low levels of plasma urea than boys (p <10−4) (Table 4). Across teenage years, plasma urea levels differed suggestively from ages 11–12 years and 15–16 years in boys (respective p = 0.04, 0.006), however only between ages 15–16 years in girls (p = 0.007) (S3 and S4 Tables).

Table 4. Status of nitrogen metabolites in adolescent boys and girls with age.

Uric acid.

Plasma levels of uric acid were also remarkably low in girls than boys across all ages during adolescence (p < 0.005, 11 years and p < 10−4, 12 to 17 years) (Table 4). With each successive year in age from 11 years to 15 years, plasma uric acid levels increased in boys (p ≤ 10−4). On contrary, uric acid levels were seen to rise significantly only during transition from 11–12 years in girls (p = 0.005) (S3 and S4 Tables).


Together with urea and uric acid, Indian girls had apparently low levels of plasma creatinine than boys, with significant difference especially during the late adolescence period than boys (p < 10−4) (Table 1). In boys, creatinine levels were consistently found to be increased from age of 12 years to 16 years, with highest increment between 13 to 15 years (p < 10−6) (Table 4, S3 Table). Girls did not show a remarkable upsurge in creatinine levels during adolescence wherein levels increased only marginally from ages 11 to 15 years (p < 0.05).

BMI status significantly governs blood biochemistry during adolescence

We categorized our samples as per their BMI z-scores and observed that levels of all biochemical parameters except urea alter drastically among OB, OW and NW adolescents (Table 5). Similar trend of association was also noted for all parameters in the respective BMI categories within each gender (Table S5a and S5b) barring FG, which however showed consistency when investigated individually in boys and girls.

Table 5. Variation of biochemical parameters with BMI during adolescence.


Rapid growth and development during childhood administer normal circulating levels of various biochemical markers. Abnormal levels of such markers are classical indications that can potentially signal an underlying pediatric disease or early signs of an adult onset disease. These levels are strongly influenced by their rate of growth, organ maturity, hormone responsiveness, nutrition and metabolism [31, 32]. Additionally, children are immunologically naive and respond differently to infections, therefore adult normative values of various biochemical markers are not applicable in a pediatric setting. Given this background, we aimed to establish normative range of commonly studied blood biochemical parameters in Indian adolescents.

This is the first comprehensive study in 7,618 Indo–Europeans and a largest one worldwide ever conducted to examine blood biochemistry and its variation with gender and age during adolescence. Previously, Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) compiled health information from nearly 9000 community children of all ages from birth to 18 years but comprised only a handful of adolescents [33]. Likewise, the BCAMS Study in Chinese population inspected 3,223 schoolchildren aged 6–18 years [34]. Earlier studies investigating blood biochemistry specifically in adolescents were conducted in limited samples—Nigerians (N = 628) [35], Croatians (N = 998) [36] and Czech (N = 1,518) [37], which is much narrow than the present study. In India, a previous study examined a few surrogate markers for insulin resistance in 695 adolescents [38] and serum lipid profile in 4,245 children and adolescents aging from 6–17 years [39] but failed to deliver normative ranges for all common blood biochemical parameters assessed in a large sample set that could represent Indian population.

In accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines [40], we studied various parameters in an interval between 0.025 and 0.975 fractiles of the distribution of results wherein 95% of the values resided. In adolescents, normative range for fasting plasma glucose of 3.56–6.49mmol/L was observed in Indians as against 3.77–8.27mmol/L in Nigerians [35] and 3.9–5.9mmol/L in Croatians [36]. For plasma insulin, Indian adolescents have normal levels within a range of 10.60–199.48pmol/L that is typically less than reported by a recent multiethnic study in Canada–CALIPER (15 – 345pmol/L) [33]. Mean plasma glucose and insulin levels were found to be 4.8mmol/L and 68.90pmol/L in the present study in lieu of previously reported levels of 5.19mmol/L and 87.5pmol/L in mere 695 Indian adolescents [38]. Besides, Indian boys featured considerably higher plasma glucose levels but lower plasma insulin than girls. This agrees with previous studies in normal weight adolescents from Czech [37] and Chinese populations [34] but confronts CALIPER data reporting similar insulin levels in both genders [33]. Girls are more insulin resistant than boys [41]. Adolescence period is largely predominated by puberty that is associated with a marked decrease in insulin sensitivity [42] that in turn is accompanied by compensatory increase in insulin secretion eventually leading to insulin resistance [43]. This provides a biological rationale for presence of remarkably high insulin and correspondingly low glucose levels in girls during adolescence.

Normative levels of C–peptide (0.21–3.22nmol/L) in Indians encompassed larger confines than reported levels in Canadians (0.26–2.24nmol/L) [33]. Besides insulin, Indian girls showed significantly higher C–peptide levels than boys. C–peptide, a product of insulin biosynthesis, is released in equimolar amount as mature insulin [44]. In view of higher secretion of insulin in girls, higher levels of C–peptide were anticipated [44, 45, 46]. Furthermore, mean HbA1c levels in Indians (5.16%) resembled Mexican–Americans (5.05%), non–Hispanic blacks (5.07%) and non–Hispanic whites (4.93%) [47]. Despite low glucose levels, we noticed unusually high HbA1c in girls during adolescence. Plasma glucose molecules attach non–enzymatically to hemoglobin in erythrocytes (HbA1c) [48]. Any variation in hemoglobin levels due to erythrocyte turnover will influence HbA1c values regardless of glucose levels. During adolescence, there is an upsurge in release of sex–specific hormones–estrogen in girls and testosterone in boys. Higher testosterone synthesis in boys at puberty results in greater production of hemoglobin [49]. HbA1c is the ratio of glycated hemoglobin to total hemoglobin content. Hence, a higher amount of total hemoglobin in boys will end up in lower HbA1c levels than girls which is what we also observed in our study.

Levels of total cholesterol in Indian adolescents were found to lie between 2.49–5.54mmol/L that is correspondent to well–established levels (2.9–5.8mmol/L) in children and adolescents from multiple ethnicities in Canada [33]. We observed mean levels of TC as 3.78mmol/L that is marginally lower than previously reported TC levels (4.85mmol/L) in Indian adolescents [38]. Further, presence of high TC levels in girls than boys during adolescence is also detected in Croatians where girls display normal TC levels within 3.3–6.2mmol/L as against 2.8–5.6mmol/L in boys [36]. Identification of high TC in girls is also noticed in Chinese adolescents [50]. In each gender, TC levels remained constant across different ages during adolescence. This finding is however inconsistent with the results in US adolescents that presented a decrease in TC levels with sexual maturation [51]. It is well–established that during puberty plasma lipid levels tend to differ by gender, ethnicity or both [51]. Correspondingly normal LDL levels in Indian adolescents were found to be similar between genders ranging from 1.16–3.69mmol/L. Similarity in LDL levels between girls and boys is also seen in Europeans ({Boys: 1.5–4.1mmol/L}; {Girls: 1.6–4.3mmol/L }) [36] and previously studied Indians ({Boys: mean levels = 2.20mmol/L}; {Girls: mean levels = 2.22mmol/L}) [38]. After the age of 13 years (peak pubertal period), girls showed higher LDL levels than boys. This nicely agrees with the observations in 14-year-old Chinese adolescents (girls: 1.03–3.41mmol/L; boys: 0.97–2.99mmol/L) [50]. Puberty is accompanied by increased BMI and subcutaneous adiposity owing to abrupt rise in estrogen levels [52]. Subcutaneous adiposity is a major covariate for LDL levels in adolescents (p < 0.001) [53] which fairly accounts for raised LDL levels in girls following puberty.

Normative range of HDL levels in Indians (0.78–1.85mmol/L) is lower than previously reported limits in Europeans (0.9–2.2mmol/L) [36]. In addition, mean HDL levels were observed to be like earlier stated levels in Indian adolescents (1.22mmol/L against 1.14mmol/L) [38]. During early years of adolescence, both boys and girls harbored similar HDL levels. However, at puberty, boys uniquely encountered a radical decline in HDL levels. Essentially, male puberty is characterized by boost in testosterone production that stimulates hepatic lipase activity [54], thereby reducing plasma HDL levels during the period [55]. On the other hand, observed TG levels in Indian adolescents (0.33–2.24mmol/L) correspond to normative TG levels in children and adolescents in Canada (0.5–2.23mmol/L) [33], however disperse over a wider range than Europeans (0.3–1.6mmol/L) [36]. Mean TG levels were slightly lower than our previously reported TG levels in lesser number of Indian adolescents (1.04mmol/L than 1.56mmol/L) [38]. At all successive ages after onset of puberty (14 years), high TG levels prevailed in Indian boys. Also, in Chinese boys, TG levels were reported to significantly increase from 0.79mmol/L at 12 years to 1.00mmol/L at 18 years of age [50]. Elevated TG levels in boys during adolescence occurs due to testosterone mediated decrease in Post Heparin (PH) Lipoprotein Lipase [56], an enzyme that catalyzes hydrolysis of TG [57].

Serum urea, uric acid and creatinine levels in Indian adolescents were observed to commonly reside within respective limits of 3.56–11.45mmol/L; 130.01–440.15μmol/L and 22.99–74.28μmol/L that are wider in comparison to 2.7–6.8mmol/L, 125–228μmol/L and 46–80μmol/L in Europeans [36]. All three studied nitrogen metabolites revealed exceptionally high levels in boys than girls throughout adolescence. Urea is a chief nitrogenous waste product of dietary proteins [58]. Protein rich foods largely comprise fish, meat, poultry and eggs. We recruited our samples from Northern part of India that is mainly dominated by vegetarian population [21, 59]. Here, men are primary meat consuming people [21, 59] and therefore vindicates high urea levels in boys. Similarly, dietary intake of high amounts of purine rich foods–red meat, sea foods, mushrooms, etc. end up in high uric acid levels in blood plasma [60]. At odds with fundamental influence of diet on plasma levels of urea and uric acid, creatinine is a waste product synthesized by muscles at a steady rate as a part of normal daily activity [61]. Men have higher muscle mass than women throughout their life [61] which corresponds to high creatinine production and elevated levels in blood.

Besides drastic variation in blood biochemistry due to multiple physiological events, a higher BMI further reinforces this variability during adolescence. We observed that all biochemical parameters vary considerably among Obese/ overweight and lean adolescents. Urea was noted to be an exception to the prevalent trend. The underlying rationale behind consistency of serum urea levels with obesity is though unknown, however urea levels has been previously been reported to remain unaltered with weight loss [62].

As is now evident that normal blood biochemistry indeed changes with ethnicity and food habits, our study therefore established a population specific normative range of common blood biochemical parameters in Indian adolescents and investigated its variability with gender, age and BMI. The age of onset of puberty varies widely among individuals owing to their genetic and environmental factors starting from as early as 8 years to as late as 16 years. The precise interpretation of our data is confined by lack of information about the age of onset of puberty in our cohort. Our findings will benefit medical practitioners assessing pediatric and adolescent health in piloting a timely intervention for chronic conditions and also serve as a platform for future studies to design proper reference intervals for adolescents in different world populations.

Supporting information

S2 Table. Calculated p values for comparison of various biochemical parameters between boys and girls of same age group.


S3 Table. Calculated p values for comparison of various biochemical parameters among boys of different age.


S4 Table. Calculated p values for comparison of various biochemical parameters among girls of different age groups.


S5 Table. Variation of biochemical parameters with BMI in boys and girls during adolescence.



We thank all adolescents and their parents for participation and school authorities for cooperation in carrying out the study. We also thank all the members of INDICO Consortium: Viswanathan Mohan, Saurabh Ghosh, Abhay Sharma, Rubina Tabassum, Ganesh Chauhan, Anubha Mahajan, Om Prakash Dwivedi, Lakshmi Ramakrishnan, Radha Venkatesan, M. Chidambaram, D. Prabhakaran, K.S Reddy, Monisha Banerjee, Madhukar Saxena, Sandeep Mathur, Anil Bhansali, Viral Shah, S.V. Madhu, R.K. Marwaha, Pradeep Venkatesh, S.K. Aggarwal, Shantanu Sen Gupta, Sreenivas Chavali, Amitabh Sharma, Analabha Basu, Priyanka Banerjee, Anil K Giri, Shraddha Chakraborty, Yasmeen Kauser, Gauri Prasad, B. Abitha, Aditya Undru, Donaka Rajashekar, Vaisak Parekatt, Suki Roy and Anjali Singh for their support in sample collection, processing and data generation.


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