Skip to main content
Browse Subject Areas

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Biodiversity of cultivable Burkholderia species in Argentinean soils under no-till agricultural practices

  • Walter Omar Draghi ,

    Roles Conceptualization, Funding acquisition, Writing – original draft, Writing – review & editing;

    Affiliations Fundación Instituto Leloir, IIBBA CONICET, Buenos Aires, Argentina, Instituto de Biotecnología y Biología Molecular–CCT La Plata CONICET, Universidad Nacional de La Plata, La Plata, Argentina

  • Jose Degrossi,

    Roles Conceptualization, Methodology, Supervision, Writing – review & editing

    Affiliation Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina

  • Magalí Bialer,

    Roles Formal analysis, Investigation, Writing – review & editing

    Affiliation Fundación Instituto Leloir, IIBBA CONICET, Buenos Aires, Argentina

  • Graciela Brelles-Mariño,

    Roles Formal analysis, Writing – review & editing

    Affiliation Center for Research and Development of Industrial Fermentations, (CINDEFI, CCT-LA PLATA-CONICET), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina

  • Patricia Abdian,

    Roles Conceptualization, Formal analysis, Investigation, Writing – review & editing

    Affiliation Fundación Instituto Leloir, IIBBA CONICET, Buenos Aires, Argentina

  • Alfonso Soler-Bistué,

    Roles Conceptualization, Formal analysis, Investigation, Writing – review & editing

    Affiliation Fundación Instituto Leloir, IIBBA CONICET, Buenos Aires, Argentina

  • Luis Wall,

    Roles Conceptualization, Supervision, Writing – review & editing

    Affiliation Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina

  • Angeles Zorreguieta

    Roles Conceptualization, Funding acquisition, Investigation, Writing – review & editing;

    Affiliation Fundación Instituto Leloir, IIBBA CONICET, Buenos Aires, Argentina


No-tillage crop production has revolutionized the agriculture worldwide. In our country more than 30 Mha are currently cultivated under no-till schemes, stressing the importance of this management system for crop production. It is widely recognized that soil microbiota is altered under different soil managements. In this regard the structure of Burkholderia populations is affected by soils management practices such as tillage, fertilization, or crop rotation. The stability of these structures, however, has not been evaluated under sustainable schemes where the impact of land practices could be less deleterious to physicochemical soils characteristics. In order to assess the structure of Burkholderia spp. populations in no-till schemes, culturable Burkholderia spp. strains were quantified and their biodiversity evaluated. Results showed that Burkholderia spp. biodiversity, but not their abundance, clearly displayed a dependence on agricultural managements. We also showed that biodiversity was mainly influenced by two soil factors: Total Organic Carbon and Total Nitrogen. Results showed that no-till schemes are not per se sufficient to maintain a richer Burkholderia spp. soil microbiota, and additional traits should be considered when sustainability of productive soils is a goal to fulfil productive agricultural schemes.


No-tillage crop production has revolutionized modern agricultural systems mostly due to the several benefits regarding soil conservation, such increasing the amount of water holding capacity, decreasing soil erosion, or increasing the amount and variety of life in soil, among others [1]. The use of this land management scheme has risen worldwide in the last years, especially since the introduction of herbicide resistant crops, which allowed a better weed control [2]. Argentina is not the exception to this phenomenon. Nowadays, more than 30 Mha are cultivated under no-tillage, stressing the importance that this practice has on the local agricultural production system (Asociación Argentina de Productores en Siembra Directa (AAPRESID);

It is recognized that soil microbiota is altered under different soil managements for crop production. Previous reports showed that agricultural practices such as fertilization, crop rotation, manure amendment or pesticide application influence the biodiversity of soil prokaryotes in their habitats [3,4,5,6]. These practices could lead to a loss of soil biodiversity, which in turn impact ecosystem’s properties that could be useful for sustainable crop production, such as disease suppressiveness, resistance and resilience to abiotic stresses, and nutrient cycling [7,8]. In this sense, the use of sustainable agricultural practices which preserve biological diversity is essential to ensure long term schemes of soil use for crop production.

The genus Burkholderia is a group of β-proteobacteria commonly found in nature and regularly associated to higher organisms such as plants, fungi, insects, or mammalians [9]. Burkholderia spp. is a common inhabitant of the soils and several species confer beneficial traits to plants improving their fitness against biotic or abiotic stressful conditions, through nitrogen fixation in legume nodules, endophytic lifestyles in roots plants, the synthesis of auxins, the modulation of ethylene levels, the biocontrol of soil-borne diseases, the induction of plant defense response, or the synthesis of siderophores [10,11,12,13]. Although several Burkholderia exhibit potential biological applications, some relevant species belong to the Burkholderia cepacia complex (BCC), a set of 20 genetically closed related species usually found in cystic fibrosis and other immunocompromised patients [14]. Interestingly, several isolates belonging to the BCC also depict plant growth promoting mechanisms, such as the nitrogen-fixing Burkholderia vietnamiensis, or Burkholderia ambifaria and Burkholderia cepacia, which promote plant growth through auxin synthesis or siderophore production respectively, while Burkholderia pyrrocinia exert biocontrol activity through the synthesis of the antifungal compound pyrrolnitrin [13,15,16,17,18,19]. Thus, the abundance and diversity of Burkholderia spp. species could strongly influence the soil productivity through the several biological functions that positively act on crop development.

The taxonomy of the Burkholderia genus is constantly revisited, and the genus could be categorized in three main clades [20,21]. Group I (BCC) is set up by species belonging to the Burkholderia cepacia Complex, as well as phylogenetically related species, showing pathogenic characteristics in their interaction with higher organisms. Group 2 is integrated by Burkholderia species commonly recognized as Plant Beneficial Environment strains (PBE), which exert plant-growth promotion or environmental beneficial effects through biological nitrogen fixation, biocontrol activity, phytoremediation, and other positive traits. The third group is composed by a group of recently described species, related to B. glathei (Burkholderia glathei group, BGG). Although each group was recently classified into new prokaryotes genera (PBE species belong to the Paraburkholderia genus [22], BGG species belong to the Caballeronia genus [23], while BCC species remain in the Burkholderia genus), some authors still disagree with that classification [21,24]. In fact, the International Committee on Systematics of Prokaryotes, Subcommittee for the Taxonomy of Rhizobium and Agrobacterium have recently stated the needed for a more conclusive approach to unravel the taxonomy of the Burkholderia genus [25]. Clearly, a more robust analysis is needed to define a robust taxonomy into this bacterial genus.

It is widely recognized that the land use affects the community structure of bacterial populations in agricultural soils. In particular, the presence and diversity of Burkholderia species were affected by agricultural practices, such as crop rotation, tillage, and plant species [26,27,28]. Furthermore, it has been shown that the diversity and richness of soil bacterial communities differed by ecosystem type, showing distinctive biogeographic patterns across different sites modulated mainly by edaphic factors [29,30]. In addition, contrasting agricultural practices (such as ploughing and no-till) exert severe effects on soil physical and chemical properties, e.g. bulk density, organic matter, or microbial biomass. However, the magnitude of the changes produced by conventional tillage cannot be easily extrapolated when analyzing no-till land uses (as in our case), mainly due to the magnitude of environmental changes imposed by intensive tillage systems that severely change soil properties and, concomitantly, the structure of bacterial population, as previously showed for the Pseudomonas genus [31]. In order to unraveling the influence of non-till soil management schedules on the structure of actively growing Burkholderia species, we performed an extensive analysis of the cultivable population of Burkholderia spp. on soils belonging to the main productive area of Argentina.


Geographical sites and soil sampling

Soil samples were collected in two consecutive years (2010 and 2011), both in mid-summer (February) and late winter (September) across a West-East transect in four different geographical sites of the central agricultural productive area of Argentina (S1 Fig): Bengolea and Monte Buey (Córdoba Province), Pergamino (Buenos Aires Province) and Viale (Entre Rios Province). The owners of the land gave permission to conduct the study on these sites.

The field study did not involve endangered or protected species. The information about soil physicochemical properties and the record of agricultural practices of the three sites in the previous five years (2004–2009) before the beginning of sampling is shown in Table 1. According to these records, three soil managements were defined: Sustainable (Good) Agricultural Practices (GAP) and Non Sustainable (Bad) Agricultural Practices (BAP). GAP schemes are represented by crop management systems under sustainability principles: no-till seeding soils subjected to intensive crop rotation, balanced nutrient replacement, minimized agrochemical use (herbicides, insecticides and fungicides) and presence of winter cover crops in the rotation schedules. BAP schemes correspond to agricultural management systems, also under no-till methods, but showing higher crop monoculture (represented mainly by soybean crop), low nutrient replacement and higher doses of agrochemical applied on crops (herbicides, insecticides and fungicides). Finally, Natural Environments (NE) used as references, are represented by grassland landscapes that were not cultivated at least in the last 30 years. NE sites were selected in an area of approximately 1 hectare close to the cultivated plots (less than 5 km apart).

Table 1. Soil and environmental characteristics at each sampling location.

Top soil samples (0–10 cm) were collected between sowing lines in triplicates for each treatment-site in three 5-m2 sampling points separated at least 50 m from each other. Each replicate sample of the top 10 cm of mineral soil was collected as a composite of 16–20 randomly selected subsamples. These subsamples were combined and homogenized in the field, transported to the laboratory and stored at 4°C until processing.

Determination of Burkholderia population size in soils

One gram of moist soil was suspended in sterile saline solution (0.85% NaCl) to a final volume of 10 ml. Samples were vortexed for 1 min and shaked for 30 minutes at 280 rpm and 28°C. Then, samples were immersed for 1 min in a sonication bath (40 kHz, 160 W, Testlab TB04, Argentina) and centrifuged for 5 minutes at 500 rpm. 10-fold serial dilutions were plated on PCAT (Pseudomonas cepacia, azelaic acid, tryptamine) medium and incubated for 5–7 days at 28º C. PCAT medium was formulated according to Burbage and Sasser but using citrulline (200 mg L-1) instead of tryptamine as the main nitrogen source [32]. PCAT was supplemented with cycloheximide (200 mg L-1) and crystal violet (2 mg L-1) to inhibit the growth of eukaryotic microorganisms and Gram-positive bacteria respectively [33]. To estimate the soil dry weight, 1 ml of soil suspension in triplicate were dried at 100°C until constant weight. Dry weight soil was calculated by the gravimetric method. Burkholderia spp. population counting was statistically analyzed in a mixed-model ANOVA (General Lineal Mixed Model), with treatments as fixed effect and sites as random effects, using the INFOSTAT package software [34]. Methods for Total Heterotrophes quantification were previously described by Agaras et al (2014). Shortly, soil suspensions were plated in triplicate on 1/10 (tryptone-soy agar, Biokar) to count total heterotrophic mesophilic bacteria (TH). Media was supplemented with cycloheximide (100μg/ml) to inhibit growth of fungi and yeasts. Colony counts were done after 48 h of incubation at 28°C.

Identification of Burkholderia species through recA sequencing and multilocus sequencing typing (MLST)

From each soil sample, 25 colonies from PCAT medium were transferred to Tryptone Soybean Agar medium (TSA) and incubated for 48 hs at 28°C (25 c.f.u. per soil samples (n = 4), 3 treatments, 4 sampling dates, n = 1200). A loop of bacteria from each colony was suspended in 50 µL of sterile deionized water and heated for 15 minutes at 95° C. These samples were frozen at -20°C until PCR amplification.

Genus-specific PCR of recA gene fragment was performed using primers Bur3 and Bur4 as previously described by Payne and col. [35]. The reaction mixture contained 1 U Taq polymerase (PBL, Productos Bio-Lógicos®, Argentina), 250 µM of each deoxynucleoside triphosphate, 1x PCR buffer, 1.5 mM MgCl2, 10 pmol of each appropriate oligonucleotide primer, and 2 to 4 µL of the DNA containing solution, in a 25 µl final reaction volume. Cycling was carried out in a BioRad thermal cycler (Bio-Rad Laboratories, Hemel Hempstead, UK). Approximately 2–3 µL of each PCR product was visualized by 1% agarose gel electrophoresis.

DNA fragments were purified and sequenced on both strands with the same set of primers used in the PCR amplification by the Sanger's dideoxy chain termination method ( Raw sequences from both strands of the PCR products were aligned, and a consensus sequence was derived using ClustalW [36]. For the MLST analysis, DNA samples from the selected strains were used. The primers and protocols used for DNA amplification of the seven genes was described by Spilker and col. [37]. DNA fragments were visualized and sequenced as previously described.

Phylogenetic reconstruction and species/taxon assignments of isolates

For phylogenetic tree construction, novel and selected recA reference sequences from Burkholderia spp. type strains were aligned using ClustalW, and the phylogenetic tree was inferred by the Neighbor-Joining method, using Tamura 3-parameter model as implemented in the MEGA 5 package [38]. A bootstrap confidence analysis was performed with 1000 replicates. Isolates were assigned to a Burkholderia spp. “like” taxon when their phylogenetic position clustered in a highly supported clade (≥ 90% bootstrapped values) with the recA sequence from the type strains. Multilocus sequencing typing of random-selected strains was performed as previously described [39]. Concatenated sequences of selected strains and Burkholderia spp. type strains obtained from the public database ( were aligned using ClustalW, and the phylogenetic tree was inferred by the Neighbor-Joining method, using Tamura 3-parameter model as implemented in the MEGA 5 package [38]. A bootstrap confidence analysis was performed with 1000 replicates.

Diversity index determination and statistical analysis

To determine the variation in the diversity of Burkholderia spp. populations across contrasting agricultural managements, α-diversity indexes (Shannon and Simpson Indexes) were determined through EstimateS software [40]. 100 randomizations were run for all tests. In order to increase the sample size and the power of statistical tests, samples were analyzed using agricultural managements as the main factor, as described by Figuerola et al [41] and Agaras et al [31]. The evolution of diversity indexes across treatments was analyzed through linear regression and adjusted by least square model, as implemented in GraphPrism software.

Multivariate analysis of environmental variables

The relationship between soil environmental variables and the diversity of Burkholderia spp. community was assessed using the Canonical Correspondence Analysis (CCA) [42], as implemented in Past Software [43]. Physicochemical data comprised total organic carbon (TOC), total nitrogen (Nt), extractable phosphorus (Pe), pH, and soil humidity, as determined by Duval et al [44].


Quantification of total Burkholderia population in soils

To estimate the population size of cultivable Burkholderia spp. in soils subjected to different agricultural managements, we essentially followed the procedure described by Pallud and col. [33], by plating the soil samples on a semi-selective medium (PCAT) followed by PCR amplification of the recA amplicon in Burkholderia colonies using primers (Bur3-Bur4), which have been shown to be specific for the detection of the entire Burkholderia genus [35]. Based on the presence of the specific recA amplicon, an average of 47.5% (n = 571) of the total isolates analyzed by PCR (n = 1200) were recognized as Burkholderia isolates.

The abundance of Burkholderia isolates was not significantly different among treatments across the sampling dates. Only non-farming Natural Environment treatments showed significant higher values in 3 out of 4 sampling dates (February 2010, 2011 and September 2011; Fig 1), thus reflecting the magnitude of the native Burkholderia population in the sampled soils under study. However, there was not a consistent pattern of bacterial counts in sampling sites subjected to contrasting agricultural management over the time. During the first year, only summer sampling (February 2010) showed significant differences between land uses, with BAP treatment showing higher Burkholderia counts, while winter sampling did not show statistical differences among treatments. During the second year a higher Burkholderia number in GAP treatments compared to BAP at both winter and summer seasons was observed. Therefore, the overall analysis showed that differences in similar treatments from different sampling seasons were never higher than 1 log c.f.u.g-1 dry soil, suggesting that Burkholderia abundance in sampled soils is evenly distributed, ranging 105 c.f.u. per gram of dry soil regardless agricultural managements. It is worth mentioning that Burkholderia counts were not necessarily biased or influenced by the number of total culturable mesophilic heterotrophic bacteria, since parallel studies on the same samples showed that the amount of heterotrophic bacteria was similar for all sites and treatments, with an average of 6.2 log10 c.f.u g-1 [31,45] (S1 Fig).

Fig 1. Quantification of Burkholderia species in Argentinian soils under contrasting agricultural management.

Each group represents different time-scales where samples were obtained. Similar letters (A, B or C) do not differ at 5% level (LSD test, p<0.05).

Structure of Burkholderia population.

To determine the composition of the culturable Burholderia population in the different treatments at the species level, sequencing of the recA amplicon from all the 557 isolates was performed. In this sense, the phylogenetic information provided by the recA gene was sufficient for the identification of Burkholderia at the genus and species level. However, a more robust identification of strains belonging to the Burkholderia genus is carried out using higher resolution approaches such as the multilocus sequencing analysis of housekeeping genes, or the whole genome sequencing [37,46]. Therefore, the isolates were named as “taxon-like”, according to the position of each isolate in a booststrap-supported cluster (>90%) with the corresponding type strains (Fig 2). To test for the accuracy of this approach, we performed a multilocus sequencing typing (MLST) of random selected strains, and compared this phylogenetic assignment with the recA typing. MLST on the selected strains (four B. ambifaria-like strains, three Burkholderia sp.-like strains, and one B. lata-like strain) evidenced the same discriminatory power than the recA based approach (S2 Fig), validating the use to the recA gene for strain identification.

Fig 2. Phylogenetic analysis or recA sequences obtained from Burkholderia spp.

The isolates were ascribed to known species of Burkholderia according to their clustering pattern with the type strains (indicated by the superscript T) in highly supported clusters (bootstraps values ≥ 90%). This figure shows the identification of strains belonging to Bengolea site. Same analysis was done over the entire collection. recA sequences were deposited in the Genbank under the Accession numbers MF941496 to MF942066.

Regarding the previously discussed taxonomic criteria, we chose to keep the Burkholderia genus for all the isolates, assigning their taxonomic position according to the three described clades.

Thus, we identified 24 different Burkholderia-like species (Fig 3). The most abundant was B. ambifaria, which belongs to the BCC, representing the 51.3% of the whole strain collection. Within BCC we also found strains related to B. cepacia, B. cenocepacia, B. lata, B. gladioli and B. pyrrocinia-like species. Within PBE we observed the presence of isolates related to B. caledonica, B. caribensis, B. phymatum, B. graminis, B. phytofirmans, B. sabiae, B. terricola, B. tuberum, and B. xenovorans-like species, being B. caledonica and B. caribiensis the most abundant species, reaching up to 13% of total isolates. The less abundant species were those belonging to the BGG group: we observed the presence of strains related to B. arvi, B. calidae, B. choica, B. cordobensis, B. glathei, B. grimmiae, B. jiangsuensis, B. pedi, and B. terrestris-like species, representing ca 8% of the collection. Finally, 9.7% of the total strains could not be assigned to any recognized Burkholderia species, and were ascribed as Burkholderia sp. strains. As shown in Fig 3, the structure of the Burkholderia population varied across the different schemes imposed by the agricultural managements, a fact which led us to characterize the changing of the bacterial diversity according to the land use.

Fig 3. Structure of Burkholderia populations.

Fig 3 shows the relative abundance of species composing each treatment at the four sampling dates. Each color represents different species according to the recA identification approach.

Land use impact the Burkholderia population diversity

Alfa-biodiversity indexes were used to describe the variability of the Burkholderia population structure. Concerning this, we performed the analysis determining the evolution of the Shannon Index (H´) as an estimator of global diversity and the Simpson Index (1/D), reflecting the dominance of some species over the total population composition [47]. In order to unravel the main factor influencing the Burkholderia diversity and, concomitantly, the indexes determination, we performed a two-way Analysis of Similarity (ANOSIM) testing the significance of differences between groups of samples (regarding sites, treatments, and sampling dates). According to these results, sampling dates were the main factor influencing the biodiversity of Burkholderia samples. The analysis showed a statistically significant difference when sampling dates vs. treatments (R = 0.095; p< 0.04) and sampling dates vs. sites (R = 0.21; p < 0.0013) were analyzed, while the combination of the remaining factors (treatments vs sites, R = 0.07; p < 0.10) did not show a statistically valid comparison. In this regard, the regression analysis of the biodiversity index was accomplished on each sampling date for the three agricultural managements, which in parallel, allowed us to obtain an improved species coverage to determine unbiased diversity indexes (Table 2). Fig 4 shows the changing of diversity indexes across the different land uses. NE showed the largest biodiversity and the more evenness population structure according to the Shannon and Simpson indexes. In contrast, BAP showed the lowest values of biodiversity and the highest dominance values, while GAP treatments showed intermediates values in between NE and BAP treatments.

Fig 4. Evolution of biodiversity indexes according to land use.

The evolution of Shannon (H´) and Simpson (1/D) indexes are shown, reflecting the influence of the soil managements on Burkholderia spp. diversity. Values obtained are the mean and standard deviations of treatments for each sampling date.

Table 2. Diversity values of Burkholderia spp. populations.

Results showed an increase in dominance values in BAP respect to GAP and NE treatments. In order to evaluate changes in the Burkholderia species across treatments, we performed a SIMPER analysis. The analysis showed that B. ambifaria-like isolates increase their presence from NE to BAP treatments, with a twofold percentage increase for BAP treatments, and representing close to 40% of the Burkholderia strains isolated from this soil management (S2 Table). In addition, B. caledonica- and B. jiangsuensis-like isolates showed a higher presence in BAP treatments. In contrast, nine Burkholderia-like species were not present in BAP treatments, resulting in a loss of richness in soils subjected to this management. GAP treatments also showed nine non-detected species compared to NE treatments. However, the absent species in GAP comprised the least represented ones (ca 4% of contribution to total average population) with respect to BAP absent strains (ca 10% of contribution to total average population), which influenced differentially in the absolute values determined by the diversity indexes.

Environmental factors affecting the diversity of Burkholderia populations

In order to determine the influence of soil chemical characteristics on the abundance of Burkholderia-like taxon, we performed a Canonical Correspondence Analysis (CCA) considering five edaphic factors: Total organic carbon (TOC), total nitrogen (Nt), extractable phosphorus (Pe), pH and soil humidity (S3 Table). This method allowed us to determine the variation in the abundance of species related to the measured soils variables. The global permutation test for all constraints together showed that the relation between the analyzed variables was significant (p = 0.016, based on 999 permutations). The first two components produced by the CCA accounted for 75.75% of the explained variance. As shown in Fig 5, the CCA plot shows a net separation of Burkholderia populations submitted to different land uses mainly by the total Nitrogen and total Organic Carbon content. This is indicated by the length of an environmental parameter line in the ordination plot, which displays the strength of the relationship of that parameter to community composition. The analysis showed that the soil composition, which is characterized by differential nutrient availability at each treatment, affected the structure of Burkholderia populations.

Fig 5. Multivariate analysis of Burkholderia spp. diversity according to soil properties.

Lines indicate the magnitude of soil measured variables associated with bacterial community structures. Treatments are depicted as colored spots, and numbers indicate sampling dates (1: February 2010; 2: September 2010; 3: February 2011; 4: September 2011).


The use of no-till agricultural practices has been widely adopted by Argentinean farmers, reaching ca 80% of the total cultivated areas. The productivity advantages, as well as the economic benefits, are the main factors that made no-till agriculture so successful. Several studies reported on the effects that these agricultural practices have on soil biology, mainly focused in alterations of soil microbiota through metagenomics analysis [41,48,49,50,51]. The study of cultivable bacterial populations has been, however, unattended [31]. Even when this approach only works for a proportion of the whole soil microbiota, the culturability of a set of organisms allowed us not only to study the ecological aspects of this group but also to use of these organisms as a source of potential biotechnological products and biomarkers. In addition, biodiversity indexes showed enough sensitivity (to detect differences among treatments) as well as robustness (systematic behavior at different sampling points) which allows their use in the study of disruption of soil biodiversity by agricultural management system [52].

During 2009, a group of Argentinean scientists conformed the BIOSPAS, a consortium aiming at studying different aspects of the soil biology of agricultural sites subjected to differential management systems, all of them under a no-till scheme [53]. As members of the BIOSPAS Consortium, our first goal was to recognize the presence of cultivable Burkholderia species in crop productive soils as well as to unravel how its occurrence was influenced by different agricultural management methods. In this regard, we showed that culturable Burkholderia strains in agricultural soils reached slightly higher concentration than 1x105 c.f.u.g-1 dry soil in natural environments. The Burkholderia concentration seems to be similar to previous results found in other soils. Using a similar approach, Pallud et al. showed the presence of 2.4x105 c.f.u.g-1 of Burkholderia cells in soils from Southeast of France, while Salles et al. found lower concentrations of Burkholderia in acidic soils from The Netherlands, reaching 1x103 c.f.u.g-1 of soil [26,33]. This last study, however, has not expressed the colony forming units counting in the same unities (dry weight soil), so the actual number could be slightly higher in those areas. The presence of the total Burkholderia population was affected by the agricultural management imposed on different sites. NE showed a higher presence of Burkholderia species but not in a regular pattern. It is broadly accepted that land use affect the presence and diversity of the soil microbiota, an effect that is especially remarkable when pristine sites are derived for agricultural purposes [54,55,56]. However, Burkholderia species and particularly BCC species -the most abundant in our case-were characterized as r-strategists organisms [12,57]. Even when suffering high mortality rates, r-strategists organisms achieve higher growth rates, which confer them with the ability to rapidly occupy disrupted ecological niches [58]. These characteristics could contribute with a high turnover in Burkholderia population species, keeping in this way different steady states in the soil cells density according to the stress factors imposed on the habitat. This high turnover in the number of Burkholderia spp. could mask the effects of soil management, making their quantification not a valid measure for studying the impact of soil treatments on microbiological diversity.

Biodiversity Indexes clearly showed the effect that agricultural managements had on the Burkholderia structure population. The decrease of diversity of Burkholderia species, through the negative correlation in the Shannon Indexes, as well as the increase in the dominance values reflect the effect of BAP treatments on Burkholderia population structure. Therefore, we found strong evidence showing that the agricultural management impacts Burkholderia diversity on soils. Salles and colleagues showed changes in Burkholderia composition in experiments carried out in soils with different land history. They showed the effects that different agricultural practices, such as tillage, crop rotation, or fertilization had on Burkholderia biodiversity, showing that grassland sites usually preserve the most diverse environment in soils [26,27,59]. Our experimental design comprised sites with agricultural crop production under no-tillage schemes, which remove a key environmental variable responsible of diversity changes previously observed in Burkholderiales [60]. Even with a more sustainable soil use, the agricultural scheme imposed on BAP treatments—with mono-cropping, high doses of pesticides and unbalanced fertilization—was deleterious to maintain the diversity of Burkholderia species in our agricultural soils, showing the increase in the dominance of B. ambifaria- like strains and the absence of the less abundant Burkholderia species when soils were subjected to non-sustainable land use. Similar results were observed in the same experiments for cultivable Pseudomonas spp. populations, with low diversity structures on GAP and BAP treatments, with BAP showing the lowest diversity [31]. Regarding treatments, results showed that no-till schemes per se are not sufficient to maintain a richer soil microbiota, and additional traits should be considered (e.g.: doses of pesticides, balanced fertilization, crop rotation) when sustainability of productive soils is a goal to fulfil in productive agricultural schemes.

Analysis of the influence of soil composition on Burkholderia population diversity showed that biodiversity was mainly linked to Total Organic Carbon and Total Nitrogen in soils. Similar results were observed when the global bacterial diversity was analyzed on the same samples in a metagenomic study [41]. In different soils, it has been shown that the presence of Burkholderia species is correlated with their ability to degrade cellulose as a carbon source [61,62], and their presence is relevant to maintain soil organic carbon concentration through their cellulolytic activity [63]. In fact, previous analyses on the same soils samples of our study have shown that GAP treatments improve the soil organic carbon compared to BAP treatments, especially for the labile organic fractions [44,64]. Previous findings have shown that soil pH is a main determining of the Burkholderia spp. abundance across soils [65]. In our case, pH was also an important soil factor but not the main one. This could be a result of a lack of contrasting pH values across sites (media pH value = 6.13±0.3), a soil pH range in which the higher abundance of Burkholderia spp. was found [65]. Therefore, it is plausible to propose that, in our conditions, land uses that improve the soil organic content could maintain a more diverse structure of Burkholderia populations through a better availability of carbon sources.

With a rising population worldwide, food production under sustainable schemes is necessary to preserve natural resources. As part of the soil microbiota, the diversity of Burkholderia species is affected by land use even in a no-till crop system, addressing the necessity to perform sustainable agricultural schemes for crop production in order to avoid the loss of bacterial species. This is important not only from an ecological point of view, but also for crop productivity, since a low bacterial diversity could interfere in natural processes that actually occur in soils, as nitrogen fixation or natural competition against pathogenic microorganism, which support crop productivity and grains yields in a sustainable manner [66].

Supporting information

S1 Fig. Map of the geolocated sampling sites template map downloaded from


S2 Fig. Quantification of Burkholderia spp. in relation with total Heterotrophes in Argentinian soils under contrasting agricultural management.

Each group represents different time-scales where samples were obtained. Similar letters (A, B or C) do not differ at 5% level (LSD test, p<0.05).


S3 Fig. Multilocus sequencing typing of selected Burkholderia spp. strains.

The phylogenetic tree shows the phylogenetic position of selected strains supporting the recA based approach. B. ambifaria strains are marked with black circles, while remaining strains (B. lata and Burkholderia spp.) are marked with a black triangle. The phylogenetic tree was built with the concatenated sequences of Burkholderia Type Strains of the 7 housekeeping genes, obtained from the publicly available database. The evolutionary history was inferred using the Neighbor-Joining method [1]. The optimal tree with the sum of branch length = 2.31961162 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) is shown next to the branches [2]. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Tamura 3-parameter method [3] and are in the units of the number of base substitutions per site. The rate variation among sites was modeled with a gamma distribution (shape parameter = 5). The analysis involved 66 nucleotide sequences. All positions with less than 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. There were a total of 2760 positions in the final dataset. Evolutionary analyses were conducted in MEGA6 [4]. Gene sequences were deposited in the Genbank under the Accession number MF942067—MF942074 for atpD, MF942075—MF942083 for gltB, MF942084—MF942092 for gyrB, MF942093—MF942101 for lepA, MF942102—MF942110 for phaC, MF942111—MF942119 for recA, and MF942120—MF942127 for trpB.


S1 Table. Values of total culturable Burkholderia spp. present at each treatment according to sampling dates.

Values are mean of and variance of the log10 of colony forming units, expressed in dry soils weight ( soil dry weight; 1: February 2010; 2: September 2010; 3: February 2011; 4: September 2011).


S2 Table. SIMPER analysis of variation in the Burkholderia population structures according to the soil management system.

Mean abundance values are expressed as percentages (%).


S3 Table. Soil chemical variables used in the canonical corresponding analysis.

COt: total organic carbon, g kg-1; Nt: total Nitrogen (g kg-1); Pe: extractable phosphorous (mg. kg-1); Humidity: percentage (%). Values were obtained from Duval y col. [64]. Number indicate sampling dates (1: February 2010; 2: September 2010; 3: February 2011; 4: September 2011).



This work was supported by the Agencia Nacional de Promoción Científica y Tecnológica, Argentina (grants PAE 36976-PID 52 and PICT 2010/2691). We thank Prof. Dr. Claudio Valverde, from the Universidad Nacional de Quilmes, Argentina, for critically reading the manuscript and his asserted suggestions. We also thank Prof. Dr. Peter Vandamme, from the Ghent University, Belgium, for provide some of the strain sequences for the MLST analysis.


  1. 1. Triplett GB, Dick WA (2008) No-Tillage Crop Production: A Revolution in Agriculture! All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Agronomy Journal 100: S-153–S-165.
  2. 2. Nardali ET (2009) No-till farming: Effects on soil, pros and cons and potential. 1–214 p.
  3. 3. Heilmann B, Lebuhn M, Beese F (1995) Methods for the investigation of metabolic activities and shifts in the microbial community in a soil treated with a fungicide. Biology and Fertility of Soils 19: 186–192.
  4. 4. Omay AB, Rice CW, Maddux LD, Gordon WB (1997) Changes in Soil Microbial and Chemical Properties under Long-term Crop Rotation and Fertilization. Soil Sci Soc Am J 61: 1672–1678.
  5. 5. Schönfeld J, Gelsomino A, van Overbeek LS, Gorissen A, Smalla K, van Elsas JD. (2003) Effects of compost addition and simulated solarisation on the fate of Ralstonia solanacearum biovar 2 and indigenous bacteria in soil. FEMS Microbiology Ecology 43: 63–74. pmid:19719697
  6. 6. Cycon M, Piotrowska-Seget Z (2009) Changes in bacterial diversity and community structure following pesticides addition to soil estimated by cultivation technique. Ecotoxicology 18: 632–642. pmid:19444604
  7. 7. Loreau M, Naeem S, Inchausti P, Bengtsson J, Grime JP, Hector A, et al. (2001) Biodiversity and Ecosystem Functioning: Current Knowledge and Future Challenges. Science 294: 804–808. pmid:11679658
  8. 8. Krause S, Le Roux X, Niklaus PA, Van Bodegom PM, Lennon JT, Bertilsson S, et al. (2014) Trait-based approaches for understanding microbial biodiversity and ecosystem functioning. Frontiers in Microbiology 5.
  9. 9. Coenye T, Vandamme P (2003) Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5: 719–729. pmid:12919407
  10. 10. Suárez-Moreno Z, Caballero-Mellado J, Coutinho B, Mendonça-Previato L, James E, Venturi V. (2011) Common Features of Environmental and Potentially Beneficial Plant-Associated Burkholderia. Microbial Ecology: 1–18.
  11. 11. Compant S, Nowak J, Coenye T, Clement C, Ait Barka E (2008) Diversity and occurrence of Burkholderia spp. in the natural environment. FEMS Microbiol Rev 32: 607–626. pmid:18422616
  12. 12. Balandreau J, Mavingui P (2007) Beneficial interactions of Burkholderia spp. with plants. In: Coenye T, Vandamme P, editors. Burkholderia Molecular Microbiology and Genomics: Horizon Bioscience. pp. 129–153.
  13. 13. Vial L, Groleau MC, Dekimpe V, Deziel E (2007) Burkholderia diversity and versatility: an inventory of the extracellular products. J Microbiol Biotechnol 17: 1407–1429. pmid:18062218
  14. 14. Vandamme P, Dawyndt P (2011) Classification and identification of the Burkholderia cepacia complex: Past, present and future. Syst Appl Microbiol 34: 87–95. pmid:21257278
  15. 15. Ciccillo F, Fiore A, Bevivino A, Dalmastri C, Tabacchioni S, Chiarini L. (2002) Effects of two different application methods of Burkholderia ambifaria MCI 7 on plant growth and rhizospheric bacterial diversity. Environ Microbiol 4: 238–245. pmid:12010130
  16. 16. Menard A, Monnez C, Estrada de Los Santos P, Segonds C, Caballero-Mellado J, Lipuma JJ, et al. (2007) Selection of nitrogen-fixing deficient Burkholderia vietnamiensis strains by cystic fibrosis patients: involvement of nif gene deletions and auxotrophic mutations. Environ Microbiol 9: 1176–1185. pmid:17472633
  17. 17. Singh R, Malik N, Singh S (2013) Improved Nutrient Use Efficiency Increases Plant Growth of Rice with the Use of IAA-Overproducing Strains of Endophytic Burkholderia cepacia Strain RRE25. Microbial Ecology: 1–10.
  18. 18. los Santos-Villalobos S, Barrera-Galicia G, Miranda-Salcedo M, Peña-Cabriales J (2012) Burkholderia cepacia XXVI siderophore with biocontrol capacity against Colletotrichum gloeosporioides. World Journal of Microbiology and Biotechnology 28: 2615–2623. pmid:22806187
  19. 19. Schmidt S, Blom JF, Pernthaler J, Berg G, Baldwin A, Mahenthiralingam E, et al. (2009) Production of the antifungal compound pyrrolnitrin is quorum sensing-regulated in members of the Burkholderia cepacia complex. Environ Microbiol 11: 1422–1437. pmid:19220396
  20. 20. Estrada-de los Santos P, Rojas-Rojas FU, Tapia-García EY, Vásquez-Murrieta MS, Hirsch AM (2016) To split or not to split: an opinion on dividing the genus Burkholderia. Annals of Microbiology 66: 1303–1314.
  21. 21. Depoorter E, Bull MJ, Peeters C, Coenye T, Vandamme P, Mahenthiralingam E. (2016) Burkholderia: an update on taxonomy and biotechnological potential as antibiotic producers. Appl Microbiol Biotechnol 100: 5215–5229. pmid:27115756
  22. 22. Sawana A, Adeolu M, Gupta RS (2014) Molecular signatures and phylogenomic analysis of the genus Burkholderia: proposal for division of this genus into the emended genus Burkholderia containing pathogenic organisms and a new genus Paraburkholderia gen. nov. harboring environmental species. Front Genet 5: 429. pmid:25566316
  23. 23. Dobritsa AP, Samadpour M (2016) Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia and proposal of Caballeronia gen. nov. to accommodate twelve species of the genera Burkholderia and Paraburkholderia. Int J Syst Evol Microbiol 66: 2836–2846. pmid:27054671
  24. 24. Eberl L, Vandamme P (2016) Members of the genus Burkholderia: good and bad guys. F1000Res 5.
  25. 25. de Lajudie PM, Young JPW (2017) International Committee on Systematics of Prokaryotes Subcommittee for the Taxonomy of Rhizobium and Agrobacterium Minutes of the meeting, Budapest, 25 August 2016. International Journal of Systematic and Evolutionary Microbiology 67: 2485–2494. pmid:28771120
  26. 26. Salles JF, Samyn E, Vandamme P, van Veen JA, van Elsas JD (2006) Changes in agricultural management drive the diversity of Burkholderia species isolated from soil on PCAT medium. Soil Biology and Biochemistry 38: 661–673.
  27. 27. Salles JF, van Elsas JD, van Veen JA (2006) Effect of agricultural management regime on Burkholderia community structure in soil. Microbial Ecology 52: 267–279. pmid:16897309
  28. 28. Salles JF, van Veen JA, van Elsas JD (2004) Multivariate analyses of Burkholderia species in soil: effect of crop and land use history. Appl Environ Microbiol 70: 4012–4020. pmid:15240277
  29. 29. Martiny JBH, Bohannan BJM, Brown JH, Colwell RK, Fuhrman JA, Green JL, et al. (2006) Microbial biogeography: putting microorganisms on the map. Nat Rev Micro 4: 102–112.
  30. 30. Fierer N, Jackson RB (2006) The diversity and biogeography of soil bacterial communities. Proceedings of the National Academy of Sciences of the United States of America 103: 626–631. pmid:16407148
  31. 31. Agaras BC, Wall LG, Valverde C (2014) Influence of agricultural practices and seasons on the abundance and community structure of culturable pseudomonads in soils under no-till management in Argentina. Plant and Soil 382: 117–131.
  32. 32. Burbage DA, Sasser M, Lumsden RD (1982) A medium selective for Pseudomonas cepacia. Phytopathology 72.
  33. 33. Pallud C, Viallard V, Balandreau J, Normand P, Grundmann G (2001) Combined use of a specific probe and PCAT medium to study Burkholderia in soil. J Microbiol Methods 47: 25–34. pmid:11566224
  34. 34. Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW. (2011) InfoStat.
  35. 35. Payne GW, Vandamme P, Morgan SH, Lipuma JJ, Coenye T, Weightman AJ, et al. (2005) Development of a recA gene-based identification approach for the entire Burkholderia genus. Appl Environ Microbiol 71: 3917–3927. pmid:16000805
  36. 36. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22: 4673–4680. pmid:7984417
  37. 37. Spilker T, Baldwin A, Bumford A, Dowson CG, Mahenthiralingam E, LiPuma JJ. (2009) Expanded Multilocus Sequence Typing for Burkholderia Species. Journal of Clinical Microbiology 47: 2607–2610. pmid:19494070
  38. 38. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, et al. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution 28: 2731–2739. pmid:21546353
  39. 39. Spilker T, Baldwin A, Bumford A, Dowson CG, Mahenthiralingam E, LiPuma JJ. (2009) Expanded multilocus sequence typing for Burkholderia species. J Clin Microbiol 47: 2607–2610. pmid:19494070
  40. 40. Colwell RK (2013) EstimateS: Statistical estimation of species richness and shared species from samples. Version 9 and earlier. User’s Guide and application. Published at: http://purloclcorg/estimates.
  41. 41. Figuerola EL, Guerrero LD, Turkowsky D, Wall LG, Erijman L (2015) Crop monoculture rather than agriculture reduces the spatial turnover of soil bacterial communities at a regional scale. Environ Microbiol 17: 678–688. pmid:24803003
  42. 42. Legendre P, Legendre LFJ (1998) Numerical Ecology: Elsevier Science.
  43. 43. Hammer Ø, Harper DAT, Ryan PD (2001) Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4: 9–18.
  44. 44. Duval ME, Galantini JA, Martínez JM, López FM, Wall LG (2016) Sensitivity of different soil quality indicators to assess sustainable land management: Influence of site features and seasonality. Soil and Tillage Research 159: 9–22.
  45. 45. Agaras B, Wall LG, Valverde C (2012) Specific enumeration and analysis of the community structure of culturable pseudomonads in agricultural soils under no-till management in Argentina. Applied Soil Ecology 61: 305–319.
  46. 46. Peeters C, Meier-Kolthoff JP, Verheyde B, De Brandt E, Cooper VS, Vandamme P. (2016) Phylogenomic Study of Burkholderia glathei-like Organisms, Proposal of 13 Novel Burkholderia Species and Emended Descriptions of Burkholderia sordidicola, Burkholderia zhejiangensis, and Burkholderia grimmiae. Front Microbiol 7: 877. pmid:27375597
  47. 47. Magurran AE (2004) Measuring biological diversity. Oxford: Blackwell Publishing.
  48. 48. Figuerola EL, Guerrero LD, Rosa SM, Simonetti L, Duval ME, Galantini JA, et al. (2012) Bacterial indicator of agricultural management for soil under no-till crop production. PLoS One 7: e51075. pmid:23226466
  49. 49. Montecchia MS, Correa OS, Soria MA, Frey SD, García AF, Garland JL. (2011) Multivariate approach to characterizing soil microbial communities in pristine and agricultural sites in Northwest Argentina. Applied Soil Ecology 47: 176–183.
  50. 50. Collavino MM, Tripp HJ, Frank IE, Vidoz ML, Calderoli PA, Donato M, et al. (2014) nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N2 -fixing community dynamics. Environ Microbiol 16: 3211–3223. pmid:25314671
  51. 51. Rascovan N, Carbonetto B, Revale S, Reinert MD, Alvarez R, Godeas AM, et al. (2013) The PAMPA datasets: a metagenomic survey of microbial communities in Argentinean pampean soils. Microbiome 1: 21. pmid:24450949
  52. 52. Haegeman B, Hamelin J, Moriarty J, Neal P, Dushoff J, Weitz JS. (2013) Robust estimation of microbial diversity in theory and in practice. ISME J 7: 1092–1101. pmid:23407313
  53. 53. Wall LG (2011) The BIOSPAS Consortium: Soil Biology and Agricultural Production. Handbook of Molecular Microbial Ecology I. pp. 299–306.
  54. 54. Buckley DH, Schmidt TM (2001) The structure of microbial communities in soil and the lasting impact of cultivation. Microbial Ecology 42: 11–21. pmid:12035077
  55. 55. Wu T, Chellemi D, Graham J, Martin K, Rosskopf E (2008) Comparison of Soil Bacterial Communities Under Diverse Agricultural Land Management and Crop Production Practices. Microbial Ecology 55: 293–310. pmid:17619214
  56. 56. da C Jesus E, Marsh TL, Tiedje JM, de S Moreira FM (2009) Changes in land use alter the structure of bacterial communities in Western Amazon soils. ISME J 3: 1004–1011. pmid:19440233
  57. 57. Ciccillo F, Fiore A, Bevivino A, Dalmastri C, Tabacchioni S, Chiarini L. (2002) Effects of two different application methods of Burkholderia ambifaria MCI 7 on plant growth and rhizospheric bacterial diversity. Environmental Microbiology 4: 238–245. pmid:12010130
  58. 58. Andrews JH, Harris RF (1986) r- and K- selection and microbial ecology. Adv Microb Ecol 9: 99–147.
  59. 59. Salles JF, De Souza FA, van Elsas JD (2002) Molecular method to assess the diversity of Burkholderia species in environmental samples. Appl Environ Microbiol 68: 1595–1603. pmid:11916673
  60. 60. Ceja-Navarro JA, Rivera-Orduña FN, Patiño-Zúñiga L, Vila-Sanjurjo A, Crossa J, Govaerts B, et al. (2010) Phylogenetic and Multivariate Analyses To Determine the Effects of Different Tillage and Residue Management Practices on Soil Bacterial Communities. Applied and Environmental Microbiology 76: 3685–3691. pmid:20382808
  61. 61. Tardy V, Chabbi A, Charrier X, de Berranger C, Reignier T, Dequiedt S, et al. (2015) Land Use History Shifts In Situ Fungal and Bacterial Successions following Wheat Straw Input into the Soil. PLoS One 10: e0130672. pmid:26102585
  62. 62. Guo L, Zheng S, Cao C, Li C (2016) Tillage practices and straw-returning methods affect topsoil bacterial community and organic C under a rice-wheat cropping system in central China. Sci Rep 6: 33155. pmid:27611023
  63. 63. Thompson CE, Beys-da-Silva WO, Santi L, Berger M, Vainstein MH, Guimaraes JA, et al. (2013) A potential source for cellulolytic enzyme discovery and environmental aspects revealed through metagenomics of Brazilian mangroves. AMB Express 3: 65. pmid:24160319
  64. 64. Duval ME, Galantini JA, Iglesias JO, Canelo S, Martinez JM, Wall L. (2013) Analysis of organic fractions as indicators of soil quality under natural and cultivated systems. Soil and Tillage Research 131: 11–19.
  65. 65. Stopnisek N, Bodenhausen N, Frey B, Fierer N, Eberl L, Weisskopf L. (2014) Genus-wide acid tolerance accounts for the biogeographical distribution of soil Burkholderia populations. Environmental Microbiology 16: 1503–1512. pmid:23945027
  66. 66. Delgado-Baquerizo M, Maestre FT, Reich PB, Jeffries TC, Gaitan JJ, Encinar D, et al. (2016) Microbial diversity drives multifunctionality in terrestrial ecosystems. Nat Commun 7: 10541. pmid:26817514