Permanent sea-ice cover and low primary productivity in the mostly ice-covered Central Arctic ocean basins result in significantly lower biomass and density of macrobenthos in the abyssal plains compared to the continental slopes. However, little is known on bathymetric and regional effects on the macrobenthos diversity. This study synthesizes new and available macrobenthos data to provide a baseline for future studies of the effects of Arctic change on macrofauna community composition in the Arctic basins. Samples collected during three expeditions (in 1993, 2012 and 2015) at 37 stations on the slope of the Barents and Laptev Seas and in the abyssal of the Nansen and Amundsen Basins in the depth range from 38 m to 4381 m were used for a quantitative analysis of species composition, abundance and biomass. Benthic communities clustered in five depth ranges across the slope and basin. A parabolic pattern of species diversity change with depth was found, with the diversity maximum for macrofauna at the shelf edge at depths of 100–300 m. This deviates from the typical species richness peak at mid-slope depths of 1500–3000 m in temperate oceans. Due to the limited availability of standardized benthos data, it remains difficult to assess if and how the significant sea-ice loss observed in the past decade has affected benthic community composition. The polychaete Ymerana pteropoda and the bryozoan Nolella sp. were found for the first time in the deep Nansen and Amundsen Basins.
Citation: Vedenin A, Gusky M, Gebruk A, Kremenetskaia A, Rybakova E, Boetius A (2018) Spatial distribution of benthic macrofauna in the Central Arctic Ocean. PLoS ONE 13(10): e0200121. https://doi.org/10.1371/journal.pone.0200121
Editor: Delphine Lannuzel, University of Tasmania, AUSTRALIA
Received: June 9, 2018; Accepted: September 13, 2018; Published: October 17, 2018
Copyright: © 2018 Vedenin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All files of the dataset are available from the PANGAEA database (https://doi.pangaea.de/10.1594/PANGAEA.890152).
Funding: Funding of this study was provided by the Alfred Wegener Institute (Polar Regions and Coasts in a Changing Earth System = PACES II program, https://www.hzg.de/institutes_platforms/coastal_research/research_topics/paces_2/index.php.de) and by the European Research Council Advanced Grant 294757 (https://erc.europa.eu/funding/advanced-grants) to Dr. Antje Boetius. This work was partly supported by the Russian Science Foundation Grant 14-50-00095 (http://rscf.ru/en) to Dr. Andrey Vedenin, Russian Foundation for Basic Research Grant 17-05-00787 (http://www.rfbr.ru/rffi/eng) to Dr. Andrey Vedenin, Dr. Elena Rybakova and Antonina Kremenetskaia, and 18-05-60228 (http://www.rfbr.ru/rffi/eng) to Dr. Andrey Gebruk (data analysis). The funders had no role in study design, data collection, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The deepest parts of the Central Arctic, including the abyssal plains of the Nansen and Amundsen Basins separated by the Lomonossov Ridge, are among the least studied areas of the global oceans. Most surveys of Arctic benthos have been conducted on the shelf or upper slope, whereas data from the deep basins remain rare and scattered in time and space. First data on the deep-sea fauna of the Central Arctic were obtained in 1935 using trawls and dredges not suitable for collecting quantitative samples. Qualitative descriptions of the Central Arctic bathyal and abyssal fauna were made by Soviet expeditions on the vessels Sadko in 1935–1937 and F. Litke in 1955 [1,2]. First quantitative data using mini-LUBS corer and “Okean” grabs were collected in the 1970s in the Canadian Basin from the American Fletcher’s Ice Island  and the Soviet drifting station North Pole-22 . The authors reported extremely low abundances at depths >1000 m of a few individuals per m2 and hence a very low benthos biomass of around 0.04 g/m2. A recent review of the Arctic macrobenthos confirms the strong decline in biomass by over an order of magnitude from the outer shelves to the inner basins of the Arctic Ocean . The Arctic deep-sea benthos has likely evolved from shallow-water relatives inhabiting the large shelves, with cold temperatures close to freezing point prevailing across the entire depth range. To this date, the communities of the deep basins and the outer shelf share more than half of their taxa , and food limitation seems to be the main factor structuring community composition .
Today, as the rapid warming and sea ice decline are likely to change the Arctic ecosystem in its entity, comparative analyses of macrofauna community composition, diversity and abundance in space and time remain challenged by the paucity of quantitative, standardized macrofauna data. Sampling surveys were made during expeditions of the research icebreakers Ymer, Polarstern, Oden and Polar Sea in the 1990s and 2000s, but unfortunately not according to internationally standardized procedures [7–12]. Over 25 macrobenthic samples were retrieved in 1991 during the Polarstern expedition to the Nansen and Amundsen Basins [13,14], but the low volume of samples obtained caused problems in diversity detection . During the Polarstern expeditions in 1993 and 1995, nine bathymetric transects were conducted at the northern slopes of Barents, Kara and Laptev Seas from shelf to abyssal depths. Results of these surveys were partly published by ,  and . The present study provides additional analyses of legacy samples from these expeditions and adds a substantial number of new data from surveys during the sea-ice minimum in 2012 [6,17].
Patterns of bathymetric distribution of benthic fauna were previously reviewed for different temperate and tropical ocean regions [18–23]. According to generally recognised patterns, the density and the biomass of macrobenthos decrease with increasing depth due to the declining flux of particulate matter as the main food supply. In contrast, species diversity shows a parabolic pattern with a maximum at depths of 1500–3000 m [24–27]. In the Arctic, the known species diversity of around 1100 taxa does not appear to follow this pattern, and no mid-depth peak of diversity has yet been detected, potentially due to the strongly limited food supply (summarised in ).
This hypothesis is supported by recent studies of horizontal distribution patterns of macrofauna in the deep-sea Central Arctic. From the shelf to the deep-sea basins, food supply is declining not only because of increasing water depth, but also because northwards, the sun angle and the sea-ice conditions limit light availability for primary producers. Concentrations of benthic chlorophyll together with macrofaunal biomass are known to be inversely proportional to ice cover . A summary of the abundance, biomass and modelled productivity of the Central Arctic macrofauna was recently published by , covering samples from a 20-year period and a depth range of 520–5420 m. It was shown that standing stock and production of the benthic communities are several times higher under the seasonal compared to the multiyear ice zones. This correlation is commonly explained by the higher particle flux under the seasonal ice[6,17].
In the present study we aimed at further testing the effect of location, water depth, sea-ice cover and phytodetritus flux for a large standardized data set of macrofauna at high taxonomical resolution, focusing on the Eurasian slope and basin.  noted before that analytic differences among investigators make difficult or even impossible to synthesize species lists from different studies. In our study the entire set of samples was identified by the same specialists. Another aim was to test the entire data set available for any indication of decadal change in community composition between the 1990s and today, i.e. before and after the significant sea-ice decline in this region . We examined the bathymetric distribution patterns of macrofauna along the Barents and Laptev Sea slopes and the spatial distribution pattern within central parts of the Amundsen and Nansen Basins. Our hypotheses were the following: 1) Variations in macrobenthos distribution are controlled by food availability; 2) Community similarity is high across all depth zones; 3) Sea-ice retreat leads to change in the community structure.
Materials and methods
Material for this study was obtained from three expeditions. During the RV Polarstern expedition ARK-IX/4 (August-September 1993), 44 benthic stations were sampled on northern slopes of the Barents and Laptev Seas and adjacent shelf areas and the Nansen Basin. Sampling gears included the multibox-corer (MG)  and the 0.25 m2 giant box-corer (GKG). From one to fourteen subsamples of the size 0.023 m2 were taken from each MG and/or from one to four subsamples of the size 0.022 m2 from each GKG using the rectangular frame. Part of these samples was processed previously at the Zoological Museum in St. Petersburg, Russia, and results were published by . The material from additional 27 stations sampled in 1993 was kept untouched until recently. This material has now been analyzed and is used in the present study to obtain a more comprehensive set of baseline data. Information on these stations, including the number of subsamples and sampling area, are shown in Table 1; the entire dataset can be downloaded from doi.pangaea.de/10.1594/PANGAEA.890152. The uppermost 1 cm of sediment from each subsample was washed through a 0.2 mm sieve; the deeper 5 cm were washed through a 0.5 mm sieve. The washed subsamples were preserved in 4% formaldehyde solution. For additional information see .
A total of eight macrobenthic stations were collected in the Nansen and Amundsen Basins during the RV Polarstern expedition ARK-XXVII/3 (August-September 2012). Sampling of macrofauna was conducted using the multibox-corer with six to eight 0.023 m2 subsamples taken at each station . Subsamples were washed separately through a 0.5 mm mesh size sieve. Four cores were fixed with 4% formaldehyde and two to four cores (depending on the number of successfully closed cores) were fixed with 96% ethanol. Data on the biomass and density of macrobenthos based on this material were published by . Our current study adds the taxonomic analysis. For additional information on sampling procedures, see .
Samples collected during the Cruise 63 of Akademik Mstislav Keldysh (September 2015) at the base of the Laptev Sea slope were also examined. Two stations using an Okean grab sampler with 0.25 m2 sampling area  were performed with three grab replicates per station. Each grab sample was washed through a 0.5 mm sieve and fixed by 5% formalin.
For all samples retrieved in 1993 and 2012, we assessed factors governing variations in macrobenthos composition including porosity, chloroplast pigments concentrations (chlorophyll a and phaeopigments), protein and phospholipids contents, bacterial abundance and activity (esterase and lipase activity) and organic carbon (S1 Table). For methods, see , and . Samples for these parameters were obtained by multi-corer samples to retrieve the undisturbed top centimeter layer of sediment at the box core stations during the same expeditions. In addition we used the sea-ice coverage data measured at the moment of sampling at each station. Additional data from the Ocean Floor Observation System (OFOS) used in the ARK-XXVII/3 expedition allowed estimating the area of the Melosira arctica algal patches on the sediment surface (https://doi.pangaea.de/10.1594/PANGAEA.803293); for methods, see  and .
Identification of macrofauna specimens to the lowest possible taxonomical level was made in the laboratory with the help of taxonomic experts (see ‘Acknowledgments’).
All specimens of each species were counted and weighted (wet weight, ww). We weighted all specimens of each species at a time. In case of molluscs the weight was measured together with shells. Polychaetes with mucous tubes (like Spiochaetopterus typicus or siboglinids) were weighted together with tubes. Sponges and ctenostomatid bryozoans were slightly blotted to remove extra water from their suface. Density and biomass were calculated per square meter. Dominant taxa in terms of abundance were distinguished for each station. The similarity between samples was estimated using the quantitative index of Bray-Curtis. We used the non-transformed data as a measure of taxonomic abundance because of the evenness across the most abundant taxa. Similarity matrixes were used for the cluster and multidimensional scaling (MDS) analyses. Clusters were generated using the UPGMA algorithm (unweighted pair group method). Results were verified by ANOSIM analysis to reveal different station groups. Species richness was estimated as a total number of species at each station. Taxonomic diversity was estimated using the Shannon-Wiener index and the Hurlbert rarefaction index for 100 individuals. The species-individuals accumulation curves for the fauna were plotted. Spearman’s rank correlation was calculated between the environmental variables and community/species characteristics. A canonical correspondence analysis (CCA) was performed to estimate the contribution of the sediment parameters, depth and ice-extent to taxonomic composition (i.e. species distribution within and between the samples) . The differences between different years of sampling were tested by the similarity percentages routine (SIMPER). For consistency in depth zonation, only the stations taken at depths >2000 m were tested for decadal variation.
Statistical analyses were performed using the Microsoft Excel 2007 (calculating the biomass and abundance values), Primer V6  (performed MDS, clusters, ANOSIM, diversity and SIMPER analyses) and PAST3  (CCA and accumulation curves) software. Maps were generated using the PanMap software 
In total 10117 individuals (ind.) were obtained from 37 stations newly sorted for this study in a standardized manner. Most of the specimens were sampled in 1993 (9443 ind. in the 38–3234 m depth range; Table 1). New samples added 101 ind. from the 2012 campaign (3432–4381 m depth range) and 573 ind. from the campaign in 2015 (2020–2390 m depth range). The specimens were attributed to 440 taxa. The density of macrobenthos varied from 12 ind. m-2 (St. 229) to 24957 ind. m-2 (St. 31). The biomass ranged from 0.06 g ww m-2 (St. 229) to 650.53 g ww m-2 (St. 25). The list of identified taxa with density and biomass values at each station is given in S2 Table.
Depth zonation of macrobenthos communities
The non-metrical cluster analysis and multidimensional scaling revealed five distinct groups of macrobenthic communities distinguished by depth (Table 2, Fig 2). Between all zones, the maximum species overlap was 16% (Table 2). The community composition was significantly different (p-value of 0.001–0.022). The outer SHELF (38–54 m), UPPER SLOPE (73–1216 m), LOWER SLOPE (1991–3054) and PLAIN (3236–4381 m) groups were distinguished based on the Bray-Curtis similarity level (Fig 2A). We further distinguished as the fifth group the MID-SLOPE cluster (981–1216 m) from the UPPER SLOPE zone (73–577 m), because of the taxonomical peculiarity of the former (Fig 2, Table 3). The main dominants by density are shown in Table 3. Diversity characteristics of each group are given in Table 4. Species richness, contribution of each taxon to the total number of individuals, and to the total biomass within each station group are shown in Fig 3.
Cluster analysis (A) and non-metric multidimensional scaling plot (B) of all stations using the Bray-Curtis similarity index. Color indicates station group distinguished by depth. Orange–SHELF-group; red–UPPER SLOPE-group; green–MID-SLOPE-group; light-blue–LOWER SLOPE-group; dark-blue–PLAIN-group.
MID-SLOPE-group is included in the UPPER SLOPE-group.
Depth range, number of stations in each station group, density of the dominant taxa and the mean dominance level with standard deviation are shown.
Mean values of species number, abundance, biomass, Pielou evenness, Hurlbert rarefaction index for 100 individuals and Shannon-Wiener index with standard deviation are shown.
The SHELF group was characterized by the high dominance of bivalves Portlandia arctica and Ennucula tenuis and the ophiuroid Ophiocten sericeum. The biomass and density in this complex were the highest respectively, mostly owing to the bivalves (Fig 3, Table 4). This group contained only two stations. The SD-values of different community characteristics were the highest within this group (Tables 3 and 4).
The UPPPER SLOPE group encompassed 12 stations. Dominant species in terms of density and biomass in this group were the bivalve Yoldiella solidula and the polychaetes Prionospio cirrifera and Cirratulidae. Other notable species were the polychaetes Proclea graffi and Notoroctus oculatus and the cumacean Ektonodiastylis nimia. The biomass and density within this group were slightly lower than in the SHELF group, but diversity was the highest (Table 4). Polychaetes were the most abundant and diverse taxon, whereas the bryozoans and echinoderms (mainly the ophiuroids Ophiacantha bidantata and Ophiocten sericeum) contributed most to the biomass (Fig 3).
The MID-SLOPE group (four stations) was strongly dominated by the polychaete Melinnopsis arctica in terms of both the abundance and biomass. Other important species included the polychaete Tharyx sp., the bivalve Yoldiella annenkovae, the scyphozoan Stephanoscyphus and the sipunculid Nephasoma diaphanes. Crustacea was the most diverse taxon in this group (Fig 3). All diversity characteristics within the MID-SLOPE group, including the species number per station, density, biomass, ES (100) and Shannon index, were significantly lower than those in the UPPER SLOPE group (with the exception of Pielou evenness) (Table 4).
The LOWER SLOPE group included 10 stations. This group was rather uneven compared to others, and dominant species were different across stations, suggesting a substantial biodiversity variation at this water depth. The most abundant taxa included the polychaetes Galathowenia fragilis, Ophelina opisthobranchiata and Terebellides cf. atlantis and the tanaids Pseudosphyrapus serratus and Pseudotanais aff. affinis.
The PLAIN group (ten stations) was the least diverse among all station groups. The abundance, biomass, Shannon index and ES (100) were the lowest, whereas the Pielou evenness was the highest. Polychaeta was the most abundant and diverse taxon. Crustacea was more notable in terms of biomass, owing to the shrimp Hymenodora glacialis present in the catch at St. 53. This station near the Laptev Sea slope had the highest abundance, biomass and species number among the PLAIN group stations. The dominant taxa at stations of this group were different and included the polychaetes Ymerana pteropoda and Anobothrus laubieri and the sponge Thenea abyssorum. Other abundant taxon was the small bryozoan Nolella sp. Notable in samples of 2012 were scattered remains of semi-degraded ice-algae, apparently sedimented colonies of the diatom Melosira arctica.
Two stations, 25 and 229, were distinctly separate from other groups revealed by cluster analysis (Fig 2B). Station 25 of the northern Barents Sea slope within the depth range of the UPPER SLOPE group (534 m) differed from other stations by the prevalence of suspension-feeders, including Porifera, Hydrozoa, Serpulidae, Bryozoa and the ophiuroid Ophiacantha bidantata in terms of both density and biomass. The multi-box corer sample at St. 229 taken in the Nansen Basin at the depth of 4008 m (the PLAIN group depth range) contained only one colony of the bryozoan Nolella sp. and an ovum of an unknown invertebrate.
In general the biomass and density of macrobenthos distinctly decreased with depth from the shallow-most stations to the deepest (Fig 4A and 4B; Table 4). The results of the linear regression test for the abundance and biomass values are given in Table 5. The number of species, the Hurlbert rarefaction and the Shannon-Wiener index showed a maximum at the shelf break at depths ~100–300 m (Fig 5A, 5B and 5C).
Number of species (A), Hurlbert rarefaction (B) and Shannon-Wiener index (C) at each station in relation to depth (logarithmic scale).
Environmental factors governing macrobenthos distribution
Environmental data available for the sample collections of 1993 and 2012 allowed to test the correlation of total abundance of macrofauna with total chloroplastic pigments, indicating phytodetritus deposition, and with bacterial abundance in the surface sediments (Table 6). Of the individual taxa, the abundances of the tanaid Pseudotanais affinis, scaphopod Siphonodentalium laubieri and polychaete Ophelina opisthobranchiata correlated significantly in abundance with chloroplastic pigments, bacterial abundance and organic carbon concentration (Table 6). The results of canonical correspondence analysis among the deep stations (>2000 m, LOWER SLOPE and PLAIN groups) are shown in Fig 6. We chose the stations for which most of environmental variables were available for years 1993 and 2012. Stations 19, 20, 35 and 50 of the depth range 1991–2993 m demonstrated higher values of phaeopigments, bacterial abundance and organic carbon, and higher density of tanaid Pseudosphyrapus serratus, amphipod Neohela monstrosa and polychaete Terebellides cf. atlantis. The deep 2012 samples and the deepest 1993 station (53) showed low chloroplast pigments values, but higher densities of polychaetes Ymerana pteropoda, Anobothrus laubieri and bryozoan Nolella sp. (Fig 6). There was no direct correlation of these samples with the ice coverage, community composition and the abundance/biomass of distinct taxa.
Ten most abundant taxa are shown. Axis 1 significance level– 0.281; axis 2 significance level– 0.231.
Correlated pairs with p<0.05 are shown.
Species-individual accumulation curves for each station group are shown in Fig 7. The curves tend to reach the saturation in the SHELF and UPPER-LOWER SLOPE groups, whereas in the PLAIN group increasing the number of samples could significantly increase the total species numbers (Fig 7).
To assess temporal trends, we selected 19 stations from all three expeditions with some regional overlap (depth >2000 m). The mean abundance of these taxa from 1993 and 2015 samples with the standard deviation are shown in Fig 8. Some differences in the abundance of certain taxa were revealed by SIMPER-analysis, but the standard deviation overlapped, so that no reliable conclusions about temporal changes could be made.
When limiting to total biomass and abundance and including the data of  and  for the depth zone 2000–4500 and comparing all data, no significant differences between the samples within these two decades (1991–1997 vs 2012–2015) could be detected (Fig 9).
Macrobenthos of the Central Arctic Ocean is controlled by food availability
Similar to results of previous investigations that addressed factors influencing macrobenthos abundance and composition in other oceans, the present study revealed the substantial decrease of density and biomass between the seasonally ice-free shelves and the year round ice-covered basins [5,27,28]. We summarized published data on the biomass and abundance for the depth ranges used in our study (Table 7). It can be concluded that the ice-covered Central Arctic shows some of the lowest macrobenthos abundances and biomasses for the Northern Seas and the North Atlantic.
The values are arranged by latitude.
In the shelf and slope areas similar values of abundance and biomass were reported for Laptev Sea [11,15], Fram Strait , Nansen Basin and Yermak Plateau . Lower values were shown for the Lomonosov Ridge . For the Arctic Ocean higher values of biomass were shown for the Kara Sea upper slope  and Franz-Josef Land  mostly due to sponges and other suspension-feeders. One of the reasons could be the courser sediment supporting higher biomass of encrusting sedentary organisms . In the Atlantic Ocean much higher values of abundance were shown for the Mauritania slope  and for the Goban Spur area .
The global bathymetrical trends of biomass in the ocean were analyzed by . The authors reported a biomass decrease from ~4 mg C m-2 on the Arctic shelf to <2.5 mg C m-2 in the Arctic deep sea, with a higher rate of decrease in the Arctic compared to the temperate and tropical areas of Atlantic and Pacific Oceans. The present study adds new data for the Central Arctic deep basins, confirming this trend of a more rapid decrease, most likely explained by low productivity and carbon export flux.
The biomass and density values observed at the abyssal stations (0.008–1.212 g ww m-2) agree with previously obtained data from other parts of the deep-sea Arctic [3,6,12]. [13,14] reported higher biomass and density values from the western parts of the Nansen and Amundsen Basins. However, this may result from samples contaminated by boreal shallow-water organisms, such as the polychaete Magelona sp., the amphipod Jassa marmorata, the bivalve Spisula elliptica etc. [13,14].  suggested that these organisms could inhabit the pipeline system of a research vessel. After excluding those shallow-water species from the analysis, the biomass and density values appeared similar to those in our results (Table 7).
The overall decrease in abundance and biomass is connected with the food availability–the most important factor in determining the structure and function of benthic communities . The amount of chloroplast pigments is commonly used as indicator of the particle flux and food availability in benthic ecosystems. It was shown that the amount of pigments decreases from the shelf edge towards abyssal depths in the Arctic Ocean [6,46]. In our study the abundance was strongly correlated with the phaeopigments and CPE values (Table 6).
Among 10 stations taken from >3200 m depth, the highest biomass and density were registered at the southern-most St. 53, the station the closest to the continental slope base. All other stations of this depth group were situated on the abyssal plain at some distance from the slope (>100 km from the 3000-isobath). Benthic biomass and abundance declined in the direction from the continental slope base towards poorer central abyssal areas. This effect in the Central Arctic can be explained by increasing sea-ice cover and limited primary productivity from restricted light availability [6,34,46].
During the Polarstern ARK27-3 cruise, numerous deposits of fresh diatom algae Melosira arctica were observed on the seafloor, including areas of all 2012 stations except for St. 229 . This diatom was observed forming meter-long filaments on the under-ice surface in remarkable densities. Calculations showed that in the extreme year 2012 marked by the largest sea-ice melt recorded to date, algal deposition had reached an average 9 g C per m2 with Melosira contribution >85% of the carbon export. It was suggested that this significant increase in algal production could have resulted from recent climate changes affecting sea-ice and snow cover thickness, an important limiting factor for primary production [6,17,47].
These food falls of Melosira were shown to attract some of opportunistic megafaunal organisms, such as holothurians and ophiuroids . Degraded parts of Melosira were discovered in some of 2012 samples. However, no evidence of direct macrobenthic response to algal biomass export to the seafloor was observed yet, and polychaete abundances were as low in 2012 as in previous years. However, the overall significantly positive correlation between the total abundance of macrobenthos and phaeopigments volume in our samples suggests that an overall increase in the sedimentation of phytodetritus is likely to increase faunal abundance and diversity in the future. Key indicator species for an increase in algal input could be tanaid Pseudotanais affinis; the biomass of this species is strongly correlated with phaeopigment concentrations (Table 6, Fig 8).
Similarity of species composition is high across all depth zones
Previous studies of macrofauna community composition suggest a high proportion of endemic species in the Central Arctic with a large overlap in species between shallow and deep regions due to uniform temperature regime and other biogeographical factors [5,48]. Here we further investigated the hypothesis that despite large variations in abundance, a significant proportion of macrofaunal species within communities would be distributed independently of water depth in the Arctic. We used species richness and species diversity (the number of species per number of individuals) for comparison.
Species distribution in the Arctic is partially explained by the “polar emergence” of fauna (review in ).  distinguish two different phenomena termed in the literature the “polar emergence”: the extension of bathymetric ranges of recent bathyal and abyssal species in the Arctic to the shelf depths (i.e. the rise of deep-water species to shallow depths) (I) and the “evolutionary emergence and submergence” related to the colonization of different bathymetric zones in the history of shelf fauna (II). Both phenomena may increase the number of species at lower shelf–upper slope depth in the Arctic Ocean. Thus,  demonstrated that among 133 species occurring deeper than 2000 m in the Arctic, 27% can be found at depths between 200 and 1000 m and 41% at depths from 0 to 200 m (the most shallow depth of occurrence). This effect may also increase the number of species at upper slope–lower shelf depths. Our data show that 121 species found in LOWER SLOPE and PLAIN zones (e.g. deeper 2000 m, see doi.pangaea.de/10.1594/PANGAEA.890152) contribute 45% to the community composition (i.e. species representation) of MID-SLOPE zone, 47% to UPPER SLOPE and 5% to SHELF. However, beyond the similarity in presence/absence, the macrofauna composition showed a substantial dissimilarity of the different depth zones.
In the present study the macrobenthos clustered significantly according to water depth, despite pronounced differences in region, time, gears and washing protocols (Fig 2). Exceptions were one station of a high current regime at the shelf edge, and one almost devoid of fauna (Nansen Basin). The statistical test with ANOSIM supported that differences between the depth ranges were significant, and higher than the differences in species composition between particular samples from different areas (i.e. Barents and Laptev seas slope). The only other extensive study of benthic community composition in the Central Arctic also detected substantial water depth zonation . This study was based on the samples of the RV Polarstern expedition ARK-XI/1 in 1995 and the IB Oden expedition Arctic Ocean-96 in 1996. Seven station groups were revealed by  on the eastern slope of the Laptev Sea, the Lomonosov Ridge and adjacent deep-sea parts of the Amundsen and Makarov Basins. The SHELF group distinguished in our study partly corresponds with the SHELF group shown by . This group is characterized by benthic communities dominated by the bivalves Ennucula tenuis and Portlandia arctica, which are widely distributed at shallow depths of the Laptev and East-Siberian Seas [11,50,51]. However, since there are only two stations within this group, we refrain from making any conclusions about community characteristics. The diverse UPPER SLOPE group in our study agrees with the HANG (i.e. Slope) and RAND (i.e. Edge or Margin) station groups of . This group is characterized by the Ophiocten sericeum and Ophiopleura borealis communities typical for the lower shelf–upper bathyal depths of the eastern Arctic [52–54]. In our study only two stations were collected on the outer shelf at depths <70 m (stations 31 and 43 forming the SHELF group) with a total sampled area 0.115 m2. The UPPER SLOPE group corresponding to the lower shelf–upper slope depths (73–577 m) consists of twelve stations with the total area sampled equivalent to 1.357 m2. It is obvious that increasing the number of stations from these depths would make conclusions more pronounced.
The MID-SLOPE group in our study matches the Melinnopsis arctica community described by  from the bathyal depths of the northern Kara Sea and the Lomonosov Ridge.  included stations from the similar depth range (981–1216 m) in the RAND group. The diverse LOWER SLOPE group in our study possibly corresponds to the RAND and RUCKEN (i.e. Ridge) communities described by .
The PLAIN group in our study matches the TIEF (i.e. Deep) group described by . The species-individual accumulation curve for the PLAIN group suggests that the number of species will sharply increase with additional samples (Fig 7). At the same time, since the density of benthos in the deep-sea Central Arctic is lower than in other parts of the ocean [6,28], it can be assumed that the small sampling area of generally used quantitative gears (0.20–0.25 m-2) is not enough for the accurate estimation of regional species diversity.
Several taxa found in the PLAIN group have not been recorded in the Central deep-sea Arctic so far. The polychaete Ymerana pteropoda was previously known from one locality north from Svalbard , one locality in the Canada Basin north from Alaska  and two localities north-east off Iceland . In our samples this species was present and even dominant in terms of density at five stations. In the genus Nolella (Bryozoa) the abyssal species were known only from the North Atlantic [58,59]. These peculiar bryozoans of a tentatively new species were present in two of our samples. These minute organisms could have been overlooked during previous investigations owing to their small size (1–3 mm) and dense silt coverage of zooids masking them among sediment particles .
We further investigated trends in macrobenthos diversity with water depth in comparison to other ocean areas. For the North Atlantic it was shown that the peak of species richness and diversity occurs at depths 2000–3000 m [26,60,61]. In the Goban Spur area of the north-east Atlantic species diversity increased with water depth from 208 m (ES(100) = 29) to 4115 m (ES(100) = 68) . At the same site, the species richness per sample showed a different pattern, since the number of species is dependent on sample area, numerical density and species diversity .  found that in Fram Strait, both species diversity and richness decreased with depth (depth range from 203 to 2977 m, ES(100) declined from 43 to 14). In the present study we detected a parabolic pattern of species diversity and richness change with depth, with the species maximum occurring at 100–300 m (ES(100) = 39 ±4), i.e. shallower than the bathymetric range investigated by  in Fram Strait.
A number of published studies on macrofauna on the Siberian shelf showed an increase of species richness and diversity from the upper shelf to the shelf edge [48,62–65]. Besides the explanation of this peak in diversity and richness by the “polar emergence” theory explained above, we also suggest that food availability by higher productivity of the marginal ice zone is a key factor. In the years 1980–2008 the seasonal ice zone was mostly found across the zones UPPER SLOPE and MID-SLOPE. Only recently it has retreated as far as beyond the BASE of the slope into the PLAIN . It remains an important task to continue surveys of potential benthic community shifts in response to the retreating sea-ice extent in the Eurasian sector of the Arctic Ocean.
The comparably lower macrobenthos richness and biomass on the Siberian Arctic shelf could be a regional phenomenon, related to the massive river run-off, potentially impacting ocean stratification and thereby food availability. The Ob, Yenisei and Lena rivers contribute almost 1700 km3 of fresh water per year into the Kara and Laptev Seas, which is about 60% of the total Arctic rivers annual discharge [67,68]. For the Kara Sea it was shown that brackish waters generated by the rivers Ob and Yenisei add to the stratification of the shelf sea and form a thick and extensive layer on the surface. The layer spreads in the form of giant lenses and can be traced even along the east coast of the Novaya Zemlya Islands . The layer of brackish waters reduces nutrient transport in summer . This may explain the lower species richness of benthos in the middle of the shelf compared to the shelf edge. In the Laptev Sea a peak in primary production related clearly to the border of the freshwater lens was found near the shelf-edge above the depths 100–200 m . Another possible reason of the lower diversity on the shallower parts of Siberian shelf could be the sea-ice scraping. Large furrows on the sediment surface left by sea ice can be traced down to 15–20 m depth . However, the influence of ice scraping on benthic macrofauna has not been studied in our work.
In the present study we synthesized and tested temporal and regional differences in benthic data in a Panarctic perspective ([5,28], this study). However, it remains currently impossible to assess potential temporal trends of the macrobenthos in response to the rapid sea-ice decline of this decade, due to the low number of benthic samples available from the Arctic Ocean (Fig 8). If temporal variation did exist, spatial variation would completely mask it. Comparison of the total abundance and biomass data revealed no differences between different years of samplings (Fig 9). However, the set of stations collected in the Central Arctic deeper than 2000 m is restricted. Repeated surveys from the same areas are needed for the correct analysis of the potential decadal changes in macrobenthos community composition in the Nansen and Amundsen basins. Furthermore, a taxonomical revision and standardizing of previously published species lists [11,15,16,73] by one pool of taxonomical experts is required.
The present work has demonstrated that on the slope of the Barents and Laptev Seas and the abyssal of the Nansen and Amundsen Basins, the macrobenthos communities group according to water depth. Five station groups were distinguished based on species composition analysis in the depth range from 38 m to 4381 m: 1) shelf (38–54 m); 2) lower shelf and upper slope (73–577 m); 3) mid-slope (981–1216 m); 4) lower slope (1991–3054 m); and 5) abyssal plain (3236–4381 m). These groups partly correspond to the groups described by  and are apparently widely distributed along the Arctic slope and abyssal plains. The distribution of the groups is strongly correlated with depth and group boundaries do not overlap bathymetrically. We partly confirmed that some variation in macrobenthos distribution is controlled by food availability. According to our data, the total macrofaunal density is correlated with the amount of phaeopigments.
The polychaete Ymerana pteropoda and the bryozoan Nolella sp. were recorded in open areas of Nansen and Amundsen Basins for the first time. Rarefaction analyses predict that the existing diversity of slope and basin macrobenthos has not been adequately sampled yet.
In this study, 37 samples collected in three different expeditions using three different sampling and washing techniques were analyzed. Despite these differences, the samples appeared to be comparable. However, more uniform sampling techniques should be used for the correct analysis including larger sampling area at each station. Identification of macrofaunal organisms should be performed by the same specialists in order to exclude misidentifications.
Decrease of density and biomass of macrobenthos with depth was confirmed for the Central Arctic Basin. The depth-related parabolic pattern of species diversity with the maximum at 100–300 m was shown for the Arctic for the first time. Temporal changes caused by thinning and retreating sea ice, which could potentially enhance primary productivity and export fluxes, are currently impossible to ground-truth given the lack of data for the Arctic Ocean deep seafloor.
S1 Table. Sediment characteristics and ice coverage at the stations https://doi.pangaea.de/10.1594/PANGAEA.890151.
We would like to thank the captains, crews and shipboard parties of the RV Polarstern expeditions in 1993 (ARK IX/4) and 2012 (ARK XXVII/3) and the RV Akademik Mstislav Keldysh expedition in 2015 (AMK-63) for multiple help with the work on board and obtaining samples. The authors thank Dr. Eike Rachor for collecting the 1993 samples, Dr. Renate Degen for the 2012 samples and Dr. Sergey Galkin for the 2015 samples. Our special thanks to taxonomic experts Dr. Dorte Janussen, Dr. Nataliya Budaeva, Dr. Vyacheslav Labay and Dr. Alexandr Mironov for help in identifying sponges, polychaetes, amphipods and echinoderms respectively. We acknowledge Dr. Christina Bienhold for providing access to the environmental datasets. We also thank Dr. Bodil Bluhm, Dr. Vadim Mokievsky and Dr. Ingrid Kröncke for informative discussions and for the data they gave us.
- 1. Gorbunov G. Bottom life of the Novosiberian shoalwaters and the central part of the Arctic Ocean. Transactions of the Drifting Expedition of the Main Administration of the Northern Sea Route on the Icebreaker "G. Sedov" 1937–1940; 1946. pp. 30–138 (in Russian).
- 2. Koltun VM. Studies of bottom fauna of the Greenland Sea and the central part of the Arctic Basin. Trudy Arcticheskogo i Antarcticheskogo Nauchno-issledovatel’skogo Instituta; 1964; 259: 13–78 (in Russian).
- 3. Paul AZ, Menzies RJ. Benthic ecology of the High Arctic. Deep Sea Mar. Biol. 1974;27: 251–262.
- 4. Moskalev LI. Benthic fauna of the outer Chikchi shelf. In: Kuznetsov AP, editor. Ecologicheskiye issledovaniya shelfa [Ecological study of shelves]; 1980: 73–79. Moscow (in Russian).
- 5. Bluhm BA, Kosobokova KN, Carmack EC. A tale of two basins: An integrated physical and biological perspective of the deep Arctic Ocean. Progr in Oceanogr. 2015;139: 89–121.
- 6. Degen R, Vedenin A, Gusky M, Boetius A, Brey T. Patterns and trends of macrobenthic abundance, biomass and production in the deep Arctic Ocean. Polar Res. 2015;34. Available from: https://www.tandfonline.com/doi/full/10.3402/polar.v34.24008.
- 7. Hoppe G, Björn-Rasmussen S, Wiberg RM. Expeditionen Ymer-80: en slutrapport. (Expedition Ymer-80: a final report.) Stockholm: Swedish Royal Academy of Sciences; 1987.
- 8. Fütterer D. ARCTIC'91: the expedition ARK-VIII/3 of RV "Polarstern" in 1991. Rep Polar Res. 1992; 107.
- 9. Fütterer D. The expedition ARCTIC'93: Leg ARK-IX/4 of RV "Polarstern" 1993. Rep Polar Res. 1994; 149.
- 10. Rachor E. Scientific Cruise Report of the Arctic Expedition ARK-XI/1 of RV "Polarstern" in 1995. Reports on Polar Research, Alfred Wegener Institute for Polar and Marine Research, Bremerhaven; 1997.
- 11. Deubel H. Structures and nutrition requirements of macrozoobenthic communities in the area of the Lomonossov Ridge in the Arctic Ocean. Rep Polar Res. 2000; 370.
- 12. Renaud PE, Ambrose WG, Vanreusel A, Clough LM. Nematode and macrofaunal diversity in central Arctic Ocean benthos. J Exp Mar Bio Ecol. 2006;330(1): 297–306.
- 13. Kröncke I. Macrobenthos composition, abundance and biomass in the Arctic Ocean along a transect between Svalbard and the Makarov Basin. Polar Biol. 1994;14(8): 519–529.
- 14. Kröncke I. Macrofauna communities in the Amundsen Basin, at the Morris Jesup Rise and at the Yermak Plateau (Eurasian Arctic Ocean). Polar Biol. 1998;19(6): 383–392.
- 15. Sirenko B, Denisenko S, Deubel H, Rachor E. Deep water communities of the Laptev Sea and adjacent parts of the Arctic Ocean. Fauna and the ecosystems of the Laptev Sea and adjacent deep waters of the Arctic Ocean. Explorations of the fauna of sea. 2004;54(62): 28–73.
- 16. Frolova EA, Anisimova NA, Frolov AA, Lyubina OS, Garbul EV, Gudimov AV. Fauna bespozvonochnykh Karskogo, Barentseva i Belogo morei (Invertebrate fauna of Kara, Barents and White Seas). Apatity: Kola Scientific Center Russian Academy of Sciences; 2003 (in Russian).
- 17. Boetius A, Albrecht S, Bakker K, Bienhold C, Felden J, Fernández-Méndez M, et al. Export of algal biomass from the melting Arctic sea ice. Science. 2013;339(6126): 1430–1432. pmid:23413190
- 18. Rowe GT, Polloni PT, Haedrich RL. The deep-sea macrobenthos on the continental margin of the northwest Atlantic Ocean. Deep Sea Res A. 1982;29(2): 257–278.
- 19. Grassle JF, Maciolek NJ. Deep-sea species richness: regional and local diversity estimates from quantitative bottom samples. Am. Nat. 1992: 313–341.
- 20. Levin LA, Gage JD. Relationships between oxygen, organic matter and the diversity of bathyal macrofauna. Deep Sea Res II. 1998;45: 129–163.
- 21. Brandt A, Schnack K. Macrofaunal abundance at 79°N off East Greenland: opposing data from epibenthic-sledge and box-corer samples. Polar Biol. 1999;22: 75–81.
- 22. Aldea C, Olabarria C, Troncoso JS. Bathymetric zonation and diversity gradient of gastropods and bivalves in West Antarctica from the South Shetland Islands to the Bellingshausen Sea. Deep Sea Res I. 2008;55(3): 350–368.
- 23. Brandt A, Linse K, Schüller M. Bathymetric distribution patterns of Southern Ocean macrofaunal taxa: Bivalvia, Gastropoda, Isopoda and Polychaeta. Deep Sea Res I. 2009;56(11): 2013–2025.
- 24. Rex MA. Community structure in the deep-sea benthos. Annu Rev Ecol Syst. 1981;12: 331–353.
- 25. Flach E, de Bruin W. Diversity patterns in macrobenthos across a continental slope in the NE Atlantic. J Sea Res. 1999;42(4): 303–323.
- 26. Gebruk AV, Budaeva NE, King NJ. Bathyal benthic fauna of the Mid-Atlantic Ridge between the Azores and the Reykjanes Ridge. J Mar Biol Assoc U.K. 2010;90(1): 1–14.
- 27. Rex MA, Etter RJ. Deep-Sea Biodiversity: Pattern and Scale. Cambridge, MA: Harvard University Press; 2010.
- 28. Bluhm AB, Ambrose WG Jr, Bergmann M, Clough LM, Gebruk AV, Hasemann C, et al. Diversity of the arctic deep-sea benthos. Mar Biodivers. 2011;41(1): 87–107.
- 29. Cochrane SK, Denisenko SG, Renaud PE, Emblow CS, Ambrose WG Jr, Ellingsen IH, et al. Benthic macrofauna and productivity regimes in the Barents Sea—ecological implications in a changing Arctic. J Sea Res. 2009;61(4): 222–233.
- 30. Parkinson CL. Global sea ice coverage from satellite data: annual cycle and 35-Yr trends. J Clim. 2014;27(24): 9377–9382.
- 31. Gerdes D. Antarctic trials with the multibox corer, a new device for benthos sampling. Polar Rec. 1990;26: 35–38.
- 32. Boetius A. The expedition of the research vessel "Polarstern" to the Arctic in 2012 (ARK-XXVII/3). Berichte zur Polar-und Meeresforschung = Reports on polar and marine research. Bremerhaven; 2013.
- 33. Lisitsyn AP, Udintsev GB. A new model of a bottom grab. Trudy Vsesoyuznogo Gidrobiologicheskogo Obshchestva. 1955;6: 217–222 (in Russian).
- 34. Boetius A, Grahl C, Kröncke I, Liebezeit G, Nöthig EM. Distribution of plant pigments in surface sediments of the Eastern Arctic. Rep Polar Res. 1996;212: 213–218.
- 35. Rossel PE, Bienhold C, Boetius A, Dittmar T. Dissolved organic carbon in sediments of the central Arctic Ocean collected during POLARSTERN cruise ARK-XXVII/3 from August–September 2012; 2015. Database: PANGAEA. Available from http://dx.doi.org/10.1594/PANGAEA.849056
- 36. McCune B, Grace JB, Urban DL. Analysis of Ecological Communities. Gleneden Beach, Oregon: Vol. Mjm Software Design; 2002.
- 37. Clarke KR, Warwick RM. Changes in marine communities: an approach to statistical analysis and interpretation. 2-nd Edition. Plymouth: PRIMER-E; 2001.
- 38. Hammer Ø, Harper DAT, Ryan PD. Past user manual. 02 April 2008. Available from: http://folk.uio.no/ohammer/past/
- 39. Diepenbroek M., Grobe H., Sieger R. Panmap. 2000. Available from: http://www.pangaea.de/Software/PanMap/
- 40. Wlodarska-Kowalczuk M, Kendall MA, Weslawski J-M, Klages M, Soltwedel T. Depth gradients of benthic standing stock and diversity on the continental margin at a high latitude ice-free site (off West Spitsbergen, 79°N). Deep Sea Res I. 2004;51: 1903–1914.
- 41. Dahle S, Anisimova NA, Palerud R, Renaud PE, Pearson TH, Matishov GG. Macrobenthic fauna of the Franz Josef Land archipelago. Polar Biol. 2009;32(2): 169–180.
- 42. Cochrane SKJ, Pearson TH, Greenacre M, Costelloe J, Ellingsen IH, Dahle S, et al. Benthic fauna and functional traits along a Polar Front transect in the Barents Sea–Advancing tools for ecosystem-scale assessments. J Mar Sys. 2012;94: 204–217.
- 43. Galeron J, Sibuet M, Mahaut ML, Dinet A. Variation in structure and biomass of the benthic communities at three contrasting sites in the tropical Northeast Atlantic. Mar Ecol Prog Ser. 2000;197: 121–137.
- 44. Flach E, Heip C. Vertical distribution of macrozoobenthos within the sediment on the continental slope of the Goban Spur area (NE Atlantic). Mar Ecol Prog Ser. 1996;141: 55–66.
- 45. Wei CL, Rowe GT, Escobar-Briones E, Boetius A, Soltwedel T, Caley MJ, et al. Global patterns and predictions of seafloor biomass using random forests. PLoS One. 2010;5(12): e15323. pmid:21209928
- 46. Klages M, Boetius A, Christensen JP, Deubel H, Piepenburg D, Schewe I, Soltwedel T. The benthos of Arctic seas and its role for the organic carbon cycle at the seafloor. In: The Organic carbon cycle in the Arctic Ocean. Berlin Heidelberg: Springer; 2004. pp. 139–167.
- 47. Fernández-Méndez M, Katlein C, Rabe B, Nicolaus M, Peeken I, Bakker K, et al. Photosynthetic production in the Central Arctic during the record sea-ice minimum in 2012. Biogeosciences. 2015;12: 3525–3549.
- 48. Renaud PE, Sejr MK, Bluhm BA, Sirenko B, Ellingsen IH. The future of Arctic benthos: Expansion, invasion, and biodiversity. Prog Oceanogr. 2015;139: 244–257.
- 49. Mironov AN, Dilman AB, Krylova EM. Global distribution patterns of genera occurring in the Arctic Ocean deeper 2000 m. Invertebrate Biol. 2013;10: 167–194.
- 50. Petryashov VV, Golikov AA, Schmid M, Rachor E. Macrobenthos of the Laptev Sea shelf. Fauna and ecosystems of the Laptev Sea and adjacent deep waters of the Arctic Ocean. Explorations of the fauna of the seas. 2004;54(62): 9–21.
- 51. Sirenko BI, Denisenko SG. Fauna of the East Siberian Sea, distribution patterns and structure of bottom communities. Explorations of the fauna of the Seas. 2010;66: 74.
- 52. Zenkevich LA. Biology of the Seas of the USSR. Moscow: Akad Nauk SSSR; 1963 (in Russian).
- 53. Smirnov AV, Smirnov IS. Echinoderms of the East Siberian Sea. Issledovaniya Fauny Morei. 1994;49(57): 132–182 (in Russian).
- 54. Piepenburg D, Schmid MK. Brittle star fauna (Echinodermata: Ophiuroidea) of the Arctic northwestern Barents Sea: composition, abundance, biomass and spatial distribution. Polar Biol. 1996;16(6): 383–392.
- 55. Holthe T. Polychaeta Terebellomorpha from the northern Norwegian Sea and the Polar Sea, with descriptions of Mugga bathyalis sp. n. and Ymerana pteropoda gen. and sp. n. Sarsia. 1986;71(3–4): 227–234.
- 56. MacDonald IR, Bluhm BA, Iken K, Gagaev S, Strong S. Benthic macrofauna and megafauna assemblages in the Arctic deep-sea Canada Basin. Deep Sea Res II. 2010;57(1): 136–152.
- 57. Parapar J, Helgason GV, Jirkov I, Moreira J. Diversity and taxonomy of Ampharetidae (Polychaeta) from Icelandic waters. Pol Polar Res. 2014;35(2): 311–340.
- 58. d’Hondt JL, Hayward PJ. Nouvelles récoltes de Bryozoaires Cténostomes bathyaux et abyssaux. Cah Biol Mar. 1981;22: 267–283.
- 59. d'Hondt JL. Bryozoaires des campagnes Abyplaine. Bulletin du Muséum national d'histoire naturelle. Section A, Zoologie, biologie et écologie animales. 1985;7(1): 39–48.
- 60. Rex MA. Geographic patterns of species diversity in the deep-sea benthos. In Rowe GT, editor. Deep-sea biology. New York: John Willey and Sons; 1983. pp. 453–472.
- 61. Gage JD, Lamont PA, Kroeger K, Paterson GJ, Gonzalez VJL. Patterns in deep-sea macrobenthos at the continental margin: standing crop, diversity and faunal change on the continental slope off Scotland. Hydrobiologia. 2000;440: 261–271.
- 62. Jørgensen LL, Pearson TH, Anisimova NA, Gulliksen B, Dahle S, Denisenko SG, et al. Environmental influences on benthic fauna associations of the Kara Sea (Arctic Russia). Polar Biol. 1999;22 (6): 395–416.
- 63. Gukov AY. Monitoring of Marco-Zoobenthos in the Lena River Mouth. Polarforschung. 2002;70: 107–114.
- 64. Deubel H, Engel M, Fetzer I, Gagaev S, Hirche HJ, Klages M, et al. The southern Kara Sea ecosystem: Phytoplankton, zooplankton and benthos communities influenced by river run-off. In: Ruediger S, editor. Siberian River Runoff in the Kara Sea: characterization, quantification variability and environmental significance. Elsevier, Amsterdam: Proceedings in Marine Science, 6; 2003. pp 237–275.
- 65. Vedenin AA, Galkin SV, Kozlovskiy VV. Macrobenthos of the Ob Bay and adjacent Kara Sea shelf. Polar Biol. 2015;38(6): 829–844.
- 66. Maslanik J, Stroeve J, Fowler C, Emery W. Distribution and trends in Arctic sea ice age through spring 2011. Geophys Res Lett. 2011;38: L13502.
- 67. Pivovarov SV, Hölemann JA, Kassens H, Antonow M, Dmitrenko I. Dissolved oxygen, silicon, phosphorous and suspended matter concentrations during the spring breakup of the Lena River. In: Land-Ocean Systems in the Siberian Arctic. Berlin Heidelberg: Springer; 1999. pp. 251–264
- 68. Zatsepin AG, Zavialov PO, Kremenetskiy VV, Poyarkov SG, Soloviev DM. The upper desalinated layer in the Kara Sea. Oceanology. 2010;50(5): 657–667.
- 69. Makkaveev PN, Stunzhas PA, Khlebopashev PV. The distinguishing of the Ob and Yenisei waters in the desalinated lenses of the Kara Sea in 1993 and 2007. Oceanology. 2010;50(5): 698–705.
- 70. Demidov AB, Mosharov SA, Makkaveev PN. Patterns of the Kara Sea primary production in autumn: biotic and abiotic forcing of subsurface layer. J Mar Syst. 2014;132: 130–149.
- 71. Sukhanova IN, Flint MV, Georgieva EJ, Lange EK, Kravchishina MD, Demidov AB, et al. The structure of phytoplankton communities in the eastern part of the Laptev Sea. Oceanology. 2017;57(1): 75–90.
- 72. Bluhm BA, Gradinger RR, Schnack-Schiel SB. Sea ice meio- and macrofauna 2-nd ed. In: Thomas DN, Dieckmann GS, editors. Sea Ice. Oxford: Wiley-Blackwell; 2009. pp. 149–186.
- 73. Clough LM, Ambrose WG, Cochran JK, Barnes C, Renaud PE, Aller RC. Infaunal density, biomass and bioturbation in the sediments of the Arctic Ocean. Deep Sea Res II. 1997;44(8): 1683–1704.
- 74. Bluhm BA, MacDonald IR, Debenham C, Iken K. Macro-and megabenthic communities in the high Arctic Canada Basin: initial findings. Polar Biol. 2005;28(3): 218–231.