In mountainous landscapes, soil moisture is highly dynamic due to the effects of topography and the temporal variability imposed by seasonal precipitation, including rainfall and snow. Soil moisture is known to affect ecosystem carbon exchange both aboveground and belowground, as well as the stable isotopic composition of exchanged CO2. In this study we used an extensive suite of measurements to examine the effects of seasonal changes in soil moisture on the isotopic composition of soil CO2 production at the landscape level. We show that the seasonal decline in soil moisture (i.e., summer dry-down) appeared to impose a trend in the δ13C of soil CO2 production (δP) with more negative δP early in the growing season when soils were wet, and more positive δP as the growing season progressed and soils dried out. This seemingly generalizable pattern for a snow-dominated watershed is likely to represent the variability of recently assimilated C, tracked through the plant-soil system and imprinted in the respired CO2. Thus, our observations suggest that, at least for mountainous environments, seasonal changes in δP are largely mediated by soil moisture and their spatial variability is partially organized by topography.
Citation: Riveros-Iregui DA, Lorenzo TM, Liang LL, Hu J (2018) Summer dry-down modulates the isotopic composition of soil CO2 production in snow-dominated landscapes. PLoS ONE 13(5): e0197471. https://doi.org/10.1371/journal.pone.0197471
Editor: Riikka Rinnan, University of Copenhagen, DENMARK
Received: February 18, 2018; Accepted: May 2, 2018; Published: May 10, 2018
Copyright: © 2018 Riveros-Iregui et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the paper and its Supporting Information file, including the soil CO2 data. Please contact the corresponding author Diego Riveros-Iregui (email@example.com) for specific questions.
Funding: This work was supported by the U.S. Department of Agriculture (award No. 2012-67019-19360) and by the Department of Geography at UNC-Chapel Hill.
Competing interests: The authors have declared that no competing interests exist.
The hydrological cycle plays a large role in the expression of terrestrial ecosystem processes. Climate change is expected to intensify the hydrologic cycle in several ways, including by shifting the timing of springtime snowmelt towards earlier in the year in snow-dominated regions . This phenomenon has been amply observed  and is expected to continue [3, 4] throughout the western United States. The timing and magnitude of snowmelt is not only a function of latitude, but is also strongly dependent on the physiographic characteristics of individual catchments . While peak streamflows may increase along riparian corridors early in the growing season, high and steep areas of mountainous watersheds—which comprise the greatest areal extension of mountainous ecosystems—will dry out faster .
Reduction in water availability in sloping terrain can be problematic because it may translate into changes in ecosystem function at multiple levels. For example, reduced soil moisture in snow-dominated ecosystems is known to affect ecosystem carbon exchange both aboveground [7, 8] and belowground [9, 10]. Quantifying such changes is especially challenging for belowground processes, as factors driving changes in soil CO2 production and flux are complex and often confounding [11, 12]. Stable carbon isotope analysis is a useful tool to examine the response of soil CO2 production to changes in soil moisture and other environmental conditions [13–16]. Isotopic discrimination in plants as a result of photosynthesis and post-photosynthetic transport is reflected in the stable carbon isotopic composition of soil . Similarly, the isotopic composition of soil CO2 is affected through the preferential diffusion of 12C-CO2 within the soil column as well as diffusion of atmospheric CO2 into the soil . Thus, changes in soil moisture affect both the physical and biological processes that mediate the stable carbon isotopic composition of soil CO2 production (δP).
Given the highly dynamic nature of soil moisture across mountainous landscapes , and considering further projections driven by climate change, we sought to evaluate the effects that the seasonal variability in soil moisture impose on the δ13C of soil CO2 production (δP) at the landscape level. Specifically, we focused on the summer dry-down that forested ecosystems experience across the western U.S. In a related study, Liang et al.  reported that the spatial variability of soil moisture was a strong predictor of the δ13C of soil CO2 production in a subalpine forest; however, this study focused on comparing differences in soil moisture imposed by landscape position (riparian versus upslope), and did not examine the seasonal (i.e., temporal) dynamics in δP due to soil dry-down. Nonetheless, the findings confirmed direct measurements of the physiological response of plants to spatial differences in soil moisture as observed in the foliar 13C composition  and tree growth rates [22, 23]. The limitations of this study, however, were that the direct observations were conducted in a period of little temporal variability in soil moisture (i.e., late summer) and thus the effects of temporal effects of soil moisture on the isotope content of soil CO2 were not observed. Here, we describe how temporal changes in soil moisture may affect δP at the landscape level using extensive spatial coverage of the observations. We focus on observations collected over nine weeks in a subalpine watershed of the northern Rocky Mountains. This is a considerable advancement from previous studies because, to the best of our knowledge, this is the first study to examine the seasonal effects of soil moisture on spatially-distributed measurements of δP, from the period immediately after snowmelt through late summer. We hypothesize that the soil moisture dry-down introduces a systematic change in δP and this can be observed at the landscape level. This study explicitly tests the role of topography and variable soil moisture on the isotopic composition of soil CO2 production measured at the landscape scale. Information gained through this research will aid in the interpretation of measurements from conventional eddy flux towers (which cannot resolve the spatial variability of soil or ecosystem CO2 fluxes across different landscape positions e.g., ), and contribute toward an improved mechanistic representation of terrestrial ecosystem carbon exchange in snow-dominated areas.
The study site was Stringer Creek, a subalpine watershed within the Tenderfoot Creek Experimental Forest (TCEF) in the Little Belt Mountains of Central Montana, United States. The U.S. Forest Service, Rocky Mountain Research Station (USFS-RMRS), which granted access to the site for this particular project, operates TCEF. The greater TCEF has an area of 3,591 ha, an elevation range of 1,838 to 2,421 m (average is 2,206 m), and receives an average 890 mm of precipitation annually, ranging from 595m at lower elevations to 1050mm at its upper slopes . Snow reaches its maximum rate of precipitation during the months of December and January and accounts for over 70% of total annual precipitation . Peak streamflow is snowmelt-driven and is usually observed in early June . Optimal soil water content for soil CO2 efflux has been found between 40% and 60% following snowmelt , with highest soil CO2 concentrations under 20 cm. The vegetation of this site consists of C3 plants, including lodgepole pine (Pinus contorta), Engelmann spruce (Picea eglemanni), whitebark pine (Pinus albicaulis), subalpine fir (Abies lasiocarpa), and grouse whortleberry (Vaccinium scoparium) in upland areas, and blue joint reed grass (Calamagrotis canadensis) along riparian meadows. The most dominant soil groups are loamy, skeletal, mixed Typic Cryochrepts in upland areas and highly organic, clayey, mixed Aquic Cryoboralfs in the riparian areas . Soil bulk density ranges from 0.962 g cm-3 in riparian meadows to 0.911 g cm-3 in uplands, with no significant differences in soil bulk density or root density reported .
We report on measurements collected over nine weeks, from June 8 to August 9, 2013 at thirty-two soil plots located in Stringer Creek, a sub-watershed of TCEF. Plots were distributed throughout the 393-ha watershed with 23 of those in upland forests and 9 along riparian meadows. Soil CO2 was collected from existing gas wells installed at depths of 5, 20, and 50 cm in each plot and described in detail by a previous study . The gas wells consisted of 15-cm PVC sections of 5.25 inside diameter capped with rubber stoppers (size 11), inserted into augered holes and open at the depth of interest to allow for gas equilibrium between the gas well and the surrounding soil. PVC tubing (4.8mm inside diameter Nalgene 180 clear PVC, Nalge Nunc International, Rochester, N.Y., USA) extended from the gas wells to the ground surface. The PVC tubing was joined with connectors above the surface to prevent leaks from the gas wells between measurements. A total of 96 gas wells were used in this study (i.e., 32 plots × 3 depths).
The air space within each gas well was sampled at sub-weekly time intervals using a handheld carbon dioxide analyzer equipped with a pump (GM-70, Vaisala GM70, Woburn, MA), connected in line with a sampling valve. During gas sampling, air was circulated and monitored using the GM-70 to ensure that no sudden changes in CO2 concentration occurred inside the gas well, an indication of leaks in the system. Soil gas samples were taken usually 3–4 days apart, allowing enough time for the soil gas atmosphere to equilibrate. From each gas well, 120 ml of soil gas was extracted and injected into a 180-ml Tedlar sample bag (SKC Inc., Eighty Four, PA) that had been previously flushed with N2. Simultaneously, we measured volumetric water content of the soil at a depth of 0–12 cm (HydroSense, Campbell Scientific, UT), soil temperature (12 cm soil thermometer, Reotemp Instrument Corporation, San Diego, CA), and soil CO2 efflux (EGM-4 with SRC-1 respiration chamber, PP Systems, Amesbury, MA) three times per plot. Within 4 hr of collection, Tedlar bags were connected to a Cavity Ring-Down Spectroscopy (CRDS) analyzer (model Picarro G2101-i, Picarro Inc., Sunnyvale, California) to measure the δ13C composition of soil CO2 in the Tedlar bag. Two standard gases were used to calibrate the performance of the CRDS analyzer daily. Routine checks using these standards, instrumentation, and sampling setup yielded a repeatability of the instrument better than 0.2‰. All isotope values are reported using the delta notation and relative to the international standard of Vienna Pee Dee Belemnite (VPDB). As most groups evaluated exhibited non-normal distributions and unequal variances, we used Welch’s one-way ANOVA for group comparison .
Isotopic mixing relationships
Under steady state conditions, the δ13C of soil CO2 flux is equivalent to the δ13C of soil CO2 production . We applied the Keeling plot method  using the measured CO2 concentration and its stable isotopic composition from each depth. Thus, a Keeling plot consisted of an ordinary least-squares regression between the observed carbon isotopic compositions of the four samples (i.e., 5, 20, and 50 cm plus above air) and the inverse CO2 concentrations (i.e., 1/[CO2]) of the same four points. We used time- and site-specific measurements of above air (air above each plot at the time of sample collection). δP was calculated by subtracting 4.4‰ (diffusive depletion constant ) from the estimated intercept of each ordinary least-squares regression. Any regression with a standard error of the intercept above 1‰ was discarded, so only the most robust regressions were used for further analysis . Recent studies have shown that shallow soils are more prone to errors due to the proximity to the forest floor and the potential for artificial mixing with forest air via advection . This was confirmed by our previous work, which showed that Keeling-plot derived δP is not overly sensitive to errors at any particular depth given that it is a depth-averaged technique, and thus it provides greater confidence in the isotopic composition of soil CO2 production, particularly for surface layers . We performed the Wilcoxon rank sum test to evaluate statistical differences in soil moisture and δP across the growing season. SPSS 19 (IBM, Armonk, NY) was used to execute the Welch’s ANOVA. All other statistical analyses and data processing were performed using MATLAB R2017a (The MathWorks Inc., Natick, MA, USA).
Weekly averages of soil moisture are shown in Fig 1, separated into upland and riparian soil plots. Throughout the growing season, there was a clear decrease in soil water content across the landscape, although such a decrease is more pronounced in uplands areas where soil moisture ranges from ~25% on average in early June to less than 10% in early August. In contrast, the soil moisture in riparian areas decreased much more slowly, only reaching below 30% in the last three weeks. Given that our overarching goal was to evaluate the effects of seasonal changes in soil moisture, we used the dynamics of measured soil moisture to divide the growing season into three distinct periods: a wet period (Weeks 1–3), characterized by a slow decrease in soil moisture; a transition period (Weeks 4–6), characterized by the fastest rate of decrease in soil moisture of the growing season; and a dry period (Weeks 7–9), characterized by dry soils with little change in soil moisture. These periods and dynamics were especially evident across all upland plots (Fig 1), where these differences in soil moisture were significant across the three periods (p<0.0001 in all cases). No significant differences were found for riparian locations. Given that upland locations represent 98% of the watershed area for this catchment , the analysis below refers to these three periods.
Weekly averaged soil moisture in upland and riparian soil plots during the 2013 growing season.
Regarding soil CO2 concentrations, we found no significant differences between the CO2 concentrations between upland and riparian plots at the beginning of the growing season, although such differences did emerge as the season progressed as a result of CO2 concentrations systematically increasing in riparian plots and decreasing in upland plots (Fig 2A). On the other hand, soil CO2 flux was consistently higher in riparian plots than uplands, having its highest fluxes during the transition period (i.e., weeks 4–6) across all sites (Fig 2B).
Comparison of mean CO2 concentrations across three depths and flux between and within upland (n = 23) and riparian (n = 9) plots during wet (Weeks 1–3), transition (Weeks 4–6), and dry (Weeks 7–9) periods of the 2013 growing season. Asterisks (*) indicate significant differences between groups (p<0.05, Welch’s ANOVA). Bars indicate standard errors of the mean.
Across both landscape positions, δP was more negative during the wet period and became progressively more positive toward the transition and dry period (Fig 3). It is worth noting that these differences were statistically significant between the wet period and each of the subsequent two periods, but the differences were not statistically significant between the transition and dry periods (Fig 3), likely as a result of the increased variability among measured plots. Compared to the measured volumetric water content, the measured δP showed variable dynamics in upland plots vs. riparian plots at different times of the growing season (Fig 4). Soil moisture was a significant predictor of the variability of δP across riparian (R2 = 0.18, p<0.05) and upland plots (R2 = 0.23, p<0.0001) combined, and only during the wet period for upland plots (R2 = 0.21, p<0.05), likely as a result of a narrower range in soil moisture during the other periods (i.e., transition and dry periods). In the uplands, the range of δP (minimum – maximum value) was around ~2‰ early in the growing season when soils were wetter, whereas the same range increases to over 5‰ when soils were drier. In riparian areas these differences were not as clear.
Squares denote means across upland (n = 23) and riparian (n = 9) sites, whereas error bars denote standard deviations. Asterisks denote statistical differences between different sampling periods (p<0.05, Wilcoxon rank sum test).
Volumetric water content was a significant predictor of the variability of δP across upland (R2 = 0.23, p<0.0001) and riparian plots (R2 = 0.18, p<0.05). Note x-axis range is different in each panel.
Our experimental design took advantage of the long and pronounced summer dry-down following snowmelt at a site where snow contributes most of the water for these mountainous ecosystems , and of the monotonic decline in soil moisture throughout the growing season, especially evident in sloping areas (Fig 1). Of particular note in our results was: 1) soil CO2 concentrations increased in riparian plots as the growing season progressed, whereas the same concentrations systematically decreased in uplands; 2) the peak in soil CO2 flux occurred toward the middle of the growing season for both riparian and upland plots; and 3) the seasonal decline in soil moisture (i.e., summer dry-down) appeared to impose a trend in δP with more depleted δP early in the growing season when soils were wet, and more positive δP as the growing season progressed and soils dried out.
Plot-level observations showed that soil moisture in well-drained upland areas was significantly lower than soil moisture in riparian areas (Fig 1; one-way ANOVA, p<0.01), which can be attributed to the higher topographical position combined with the shallow, coarser soil texture relative to the poorly drained soil found in riparian plots. Across all locations, soil moisture was strongly, inversely correlated with plot elevation (r = -0.33; p<0.0001), a variable that is known to integrate important topographic characteristics such as porosity, hydraulic conductivity, aspect, clay content, specific contributing area, wetness index, curvature, and soil depth [34, 35]. And while the seasonal dry-down in soil moisture is evident across an entire growing season, it is not as evident within single 3-week periods, likely because not all sites dry down equally and the local microtopography can play a big role in the short-term dynamics or soil moisture at the site level . In addition, previous studies at this same research site have shown that wet landscape positions have higher fine root biomass and lower carbon-to-nitrogen (C:N) ratios than dry landscape positions, likely as a result of differences in vegetative cover (grasses vs. trees) . Low C:N ratios are known to contribute to rapid litter decomposition rates , whereas high root biomass can contribute to high generation and flux of soil CO2 . Both models and experimental studies have shown that soil moisture generally enhances both soil CO2 concentration and CO2 flux, although at very high soil moisture levels both gas diffusivity and production are inhibited [39–42]. Our results also suggest that for this mountainous ecosystem, soil CO2 flux is both diffusion-limited and production-limited (Fig 2). Early in the growing season during the wet period (Weeks 1–3), soil CO2 flux appeared to be diffusion-limited despite soil CO2 concentrations being slightly above 3000 ppm on average (Fig 2). Later, during the dry period (Weeks 7–9), soil CO2 flux was only slightly higher than during the wet period but significantly lower than during the transition period (Weeks 4–6) in response to soil CO2 concentrations that have increased to near 5000 ppm in riparian areas and decreased to around 2000 ppm in the uplands. In addition, it appeared that even by late summer, riparian plots remained diffusion-limited whereas upland plots were production-limited (Fig 2). These low-high-low patterns in soil CO2 flux (Fig 2B) have been previously attributed to the stimulation by soil moisture of heterotrophic and autotrophic soil CO2 production [39, 43], combined with the limitation that soil moisture imposes on soil gas transport [44, 45]. Our results appear to suggest the emergence and spatial manifestation of this well-established parabolic relationship across the landscape.
A previous study using three soil plots in a maritime pine forest  showed that the δ13C of soil CO2 flux signal became relatively more positive as the growing season progressed, coinciding with the maximum rates of soil CO2 flux. However, the same study showed greater variability after summer rainfall arrived, which was attributed to differences in the isotopic signatures of heterotrophic and autotrophic respirations and changes in their relative contribution. Separating the contribution of δ13C from heterotrophic respiration versus autotrophic respiration is notoriously difficult. Hanson et al.  reviewed over 50 published studies that used a variety of methods to partition heterotrophic versus autotrophic respiration and found that on average, root respiration contributed to 48% of total soil respiration, with this value slightly higher for forest soils. As for the heterotrophic respiration, studies show a positive relationship between increasing soil moisture and heterotrophic respiration rates , and since fractionation is assumed to be negligible during respiration  heterotrophic respired δ13C will have a similar signature to plant-derived tissue. Thus, while we recognize that heterotrophic respired δ13C may be an important and un-accounted source of CO2 to the total CO2 flux pool, the systematic increase in δP derived from analysis of 23 upland and 9 riparian plots, appears to be a generalizable pattern for this snow-dominated watershed (~4 km2 in area; Fig 3).
In water-limited ecosystems, studies have demonstrated that δ13C of whole ecosystem respiration tends to become more positive as soil moisture decreases throughout a season [24, 50] or across sites ranging in annual precipitation inputs [51, 52]. The reason is that soil moisture influences photosynthetic discrimination by C3 plants through stomatal conductance, as proposed by Farquhar et al. [53, 54]: as plants experience water limitation and stomatal conductance decreases, less discrimination against 13C by Rubisco results in more positive δ13C in the recently fixed assimilations. These enriched assimilates are then transported to other parts of the plant, where this carbon source can be used for respiration. Studies have shown that because these recently fixed sugars are transferred to the roots, δ13C of root respiration can reflect δ13C of photosynthates over relatively short time periods [13, 55]. In our study, the increase in δP with decreasing soil moisture across the growing season (Fig 4) follows this line of reasoning. Because C transport in the plant itself is not assumed to fractionate against 13C , it is likely that the observed seasonal pattern is the result of the effects of soil moisture on recently assimilated C, tracked through the plant-soil system and imprinted in the respired CO2.
Previous studies have tracked the δ13C of soil respired CO2 in C3 plants in response to day-to-day variation in the photosynthetic carbon isotope fractionation [13, 57–60], suggesting that half or more of the biological activity in the soil is driven by recent tree photosynthate. Atmospheric vapor pressure and precipitation have been reported as drivers of the δ13C of ecosystem respired CO2 [15, 51]. More recent studies, however, have found that relationships with atmospheric vapor pressure and precipitation are not consistent across the growing season at the ecosystem [24, 61] or even the soil level , making it not possible to directly relate the δ13C to plant assimilated carbon to potential variations in stomatal conductance. On the other hand, soil moisture exerts a direct control on the resulting δ13C of soil- or ecosystem-respired CO2 [20, 62–65], but summer-long patterns have been difficult to identify [24, 46], in part because the confounding effects of the multiple variables that regulate both soil moisture and leaf carbon exchange. To the best of our knowledge, this is the first study to report a generalizable pattern in the δ13C of soil CO2 production (δP) as a result of the summer dry-down of soil moisture using multiple locations in a mountainous watershed.
Soil moisture is an important factor in ecosystem CO2 exchange through its influence on physiological and soil physical processes. Our study suggests that in mountainous environments with high to medium soil water content, the summer soil moisture dry-down modulates the δ13C of soil CO2 production. We showed that the seasonal decline in soil moisture (i.e., summer dry-down) appeared to impose a trend in the δ13C of soil CO2 production (δP) with more negative δP early in the growing season when soils were wet, and more positive δP as the growing season progressed and soils dried out. This seemingly generalizable pattern for a snow-dominated watershed is likely to represent the variability of recently assimilated C in the plant-soil-atmosphere continuum. Our observations suggest that, at least for mountainous environments, seasonal changes in δP are largely mediated by soil moisture and their spatial variability is partially organized by topography. Additional research is needed to further establish the role of stochastic precipitation on masking this relationship, as well as the role of variable soil moisture on the separate isotopic contributions from heterotrophic and autotrophic respiration.
Isotopic mass balance at the ecosystem level is currently unachievable because of large gaps in our understanding of several processes, including the dynamics of leaf, plant, and soil processes in response to environmental conditions . Climate models project an increasing probability of water stress on soil and plant communities throughout the western United States due to reduction of snow accumulation. Changes in water availability at the landscape level would not only affect the rate of soil carbon turnover, but it would also affect the isotopic composition of soil CO2 production and presumably whole ecosystem respiration. Our study provides evidence to suggest that the parameterization of soil moisture heterogeneity in space and time is a required component of realistic representations carbon isotope exchange in mountainous regions.
S1 Table. Dataset used in this manuscript.
Sites T1W1, T1W2, T1E1, T1E2, T2W1, T2W2, T2E1, and T2E2 are located in riparian areas. Sites T1W3, T1W4, T1E3, T1E4, NW-, and SW- are located in upland areas.
We thank Kendra Kaiser, Erin Seybold, and William Avery for assistance in the field. We thank members of the Carbonshed Lab at UNC for suggestions on an earlier version of this manuscript. Two anonymous reviewers and the academic editor provided valuable suggestions for the improvement of this study.
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