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Open Access

Peer-reviewed

Research Article

Influence of hiatal hernia and male sex on the relationship between alcohol intake and occurrence of Barrett’s esophagus

  • Atsuhiro Masuda ,

    Roles Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Writing – original draft

    atmasuda@med.kobe-u.ac.jp

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯
  • Tsuyoshi Fujita,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Gastroenterology, Yodogawa Christian Hospital, Osaka, Japan

    ⨯
  • Manabu Murakami,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯
  • Yukinao Yamazaki,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Gastroenterology, Fukui Red Cross Hospital, Fukui, Japan

    ⨯
  • Masao Kobayashi,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Health Care, Kyoto Second Red Cross Hospital, Kyoto, Japan

    ⨯
  • Shuichi Terao,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Gastroenterology, Kakogawa Central City Hospital, Hyogo, Japan

    ⨯
  • Tsuyoshi Sanuki,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Gastroenterology, Kita-Harima Medical Center, Hyogo, Japan

    ⨯
  • Akihiko Okada,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Department of Gastroenterology, Saiseikai Nakatsu Hospital, Osaka, Japan

    ⨯
  • Masayasu Adachi,

    Roles Data curation, Supervision, Writing – review & editing

    Affiliation Hotel Okura Kobe Clinic, Hyogo, Japan

    ⨯
  • Hideyuki Shiomi,

    Roles Supervision, Writing – review & editing

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯
  • Yoshifumi Arisaka,

    Roles Supervision, Writing – review & editing

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯
  • Hiromu Kutsumi,

    Roles Methodology, Supervision, Writing – review & editing

    Affiliation Clinical Research and Medical Innovation Center, Shiga University Medical Science, Shiga, Japan

    ⨯
  • Eiji Umegaki,

    Roles Methodology, Supervision, Writing – review & editing

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯
  • Takeshi Azuma

    Roles Conceptualization, Funding acquisition, Supervision, Writing – review & editing

    Affiliation Division of Gastroenterology, Department of Internal Medicine, Kobe University Graduate School of Medicine, Hyogo, Japan

    ⨯

Abstract

Background

The association of alcohol intake with the incidence of Barrett’s esophagus (BE) has been inconsistent. Although hiatal hernia and male sex are well-known risk factors of BE, its effect on the association of alcohol intake with the incidence of BE remains unknown.

Aim

To investigate whether the influence of alcohol intake on the occurrence of BE might differ depending on male sex and presence of hiatal hernia.

Methods

We utilized a database of 8031 patients that underwent upper endoscopy for health screening in a prospective, multicenter, cohort study (the Upper Gastro Intestinal Disease study). The incidence of endoscopic columnar-lined esophagus (eCLE; endoscopically diagnosed BE) was the outcome variable. Multivariable logistic regression analysis was conducted to assess the association between alcohol intake and eCLE stratified by male sex and hiatal hernia, adjusting for clinical features and other potential confounders.

Results

Alcohol intake (≥20 g/day) showed a marginally significant association with the incidence of eCLE in participants without hiatal hernia (0 vs. ≥20 g/day; odds ratio [OR], 1.62; 95% confidence interval [CI], 0.92–2.85, P = 0.09) but not in participants with hiatal hernia (0 vs. ≥20/day; OR, 0.99; 95% CI, 0.59–1.65; P = 0.95). Furthermore, alcohol intake (≥20 g/day) was significantly associated with the incidence of eCLE in male participants without hiatal hernia (0 vs. ≥20 g/day; OR, 1.98; 95% CI, 1.04–4.03; P = 0.04) but not in female participants without hiatal hernia (0 vs. ≥20 g/day; OR, 0.47; 95% CI, 0.03–2.37; P = 0.42).

Conclusions

The effect of alcohol intake on the incidence of eCLE might be associated with hiatal hernia status and male sex.

Citation: Masuda A, Fujita T, Murakami M, Yamazaki Y, Kobayashi M, Terao S, et al. (2018) Influence of hiatal hernia and male sex on the relationship between alcohol intake and occurrence of Barrett’s esophagus. PLoS ONE 13(2): e0192951. https://doi.org/10.1371/journal.pone.0192951

Editor: Wayne A. Phillips, Peter MacCallum Cancer Centre, AUSTRALIA

Received: June 19, 2017; Accepted: February 1, 2018; Published: February 15, 2018

Copyright: © 2018 Masuda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Data Availability: All relevant data are within the paper and its Supporting Information files.

Funding: This work was supported by JSPS KAKENHI (Grants-in-Aid for Scientific Research), Grant Number 15624848 (A.M.). This study was funded by a grant provided by the NPO-Corporation, Gastrointestinal Medical Care Research and Education Center.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Barrett’s esophagus (BE) is a strong risk factor for the development of esophageal adenocarcinoma [1–4]. Some meta-analyses have shown that the pooled annual incidence of esophageal adenocarcinoma of BE was 0.19–0.33% [5, 6]. BE has malignant potential, and the determination of its epidemiology is important for preventing and screening for BE-derived esophageal adenocarcinoma.

The relationship of alcohol consumption with occurrence of BE remains controversial. Alcohol consumption causes a reduction in lower esophageal sphincter (LES) pressure and leads to gastroesophageal reflux disease (GERD) symptoms, which may induce BE [7]. Some reports have shown a significant association of alcohol consumption with occurrence of BE [8, 9], but other reports have not [10, 11]. It is important to determine the specific patient characteristics that have a significant relationship with alcohol consumption and occurrence of BE.

Evidence suggested that hiatal hernia is one of the major causes of GERD and BE [12, 13]. In hiatal hernia, the LES pressure is consistently low or weak. Hiatal hernia does not have a normally functioning LES or normal regulation of LES pressure [14]. Meanwhile, male sex is also strongly associated with the occurrence of BE [15, 16]. The anti-inflammatory action of estrogen and esophageal epithelial resistance against reflux of gastric acid was reported as its pathogenesis [17, 18]. Therefore, we investigated whether the effect of alcohol consumption might be associated with hiatal hernia and male sex.

Materials and methods

Study subjects

We utilized the database of a prospective, multicenter, cohort study (the Upper Gastro Intestinal Disease [UGID] study) of 8891 subjects enrolled in 2013–2014 who underwent upper endoscopy for health screening at seven facilities. Based on the availability of data on endoscopic columnar-lined esophagus (eCLE; endoscopically diagnosed BE), hiatal hernia, and alcohol consumption, a total of 8031 cases were included. Participants with post-total gastrectomy and those who use proton pump inhibitor and/or histamine H2-receptor antagonist were excluded from this study.

Prospective questionnaire and endoscopic findings

We collected the data from a prospective questionnaire (S1 File) including age, height, body weight, sex (male, female), current smoking (presence, absence), the current average daily alcohol consumption (0, <20, or ≥20 g/day; cutoff value of 20 g/day determined according to previous Japanese epidemiological studies) [19, 20], and heartburn and/or acid regurgitation (at any frequency in 3 months; presence, absence). Endoscopic findings were examined by experienced endoscopists in each institution, including eCLE (<10 mm, ≥10 mm), erosive esophagitis (presence, absence), hiatal hernia (presence, absence), and atrophic gastritis (presence, absence). Non-erosive reflux disease (NERD; presence, absence) was diagnosed when a subject responded that heartburn and/or acid regurgitation was present at a frequency of at least once per week in the absence of erosive esophagitis [21]. The presence of eCLE was defined as >10-mm length of columnar-lined epithelium on upper endoscopy. Endoscopic columnar-lined epithelium was diagnosed using the palisade vessels as landmark for the esophagogastric junction. An eCLE measuring less than 10 mm, reported as an “ultrashort segment” of eCLE [22], was not considered as the presence of eCLE in this study because its diagnostic criteria have been vague and there might be a high degree of inter-observer variation. Erosive esophagitis was defined by the Los Angeles classification (A-D) [23]. Presence of hiatal hernia was defined as the proximal dislocation of the gastroesophageal junction (GEJ) >2 cm above the diaphragmatic indentation. Severity of hiatal hernia was categorized by the length of the proximal dislocation of GEJ, with 2–4 cm considered as mild and >4 cm as severe. Atrophic gastritis was endoscopically diagnosed, and the endoscopic extent of atrophic mucosa was graded according to the Kimura-Takemoto classification from C-1 to O-3 [24]. Subjects with atrophic mucosa graded as C-2, C-3, O-1, O-2, and O-3 were described as positive for atrophic gastritis. This study was conducted in accordance with the Declaration of Helsinki and its amendments (UMIN-CTR ID: 000022504). The study protocol was approved by the Ethics Committee of each institution (the ethics committee of Yodogawa Christian Hospital, Fukui Red Cross Hospital, Kyoto Second Red Cross Hospital, Kakogawa Central City Hospital, Kita-Harima Medical Center, Saiseikai Nakatsu Hospital, Hotel Okura Kobe Clinic). Written informed consent was obtained from all study participants. All authors had access to the study data and had reviewed and approved the final manuscript.

Statistical analysis

All statistical analyses were conducted using JMP version 11 (SAS Institute, Cary, NC, USA), and all P values were two-sided. First, to identify the candidate risk factors, we conducted multivariable binary logistic regression analysis to assess the risk factors for the occurrence of eCLE. The binary categorical variable of eCLE (<10 mm, ≥10 mm) was used as outcome variables. Multivariable binary logistic regression analysis was performed to adjust for potential confounders. The multivariable model initially included age, body mass index, sex, current smoking, alcohol consumption, presence of heartburn or acid regurgitation, presence of erosive esophagitis, presence of hiatal hernia, presence of atrophic gastritis, and presence of NERD. A backward stepwise elimination with a threshold of P = 0.05 was used to select the variables for the final models. Then, we conducted a binary logistic model to assess the association between alcohol consumption and hiatal hernia status in relation to the occurrence of eCLE. Furthermore, we conducted a binary logistic model to assess the association between alcohol consumption and status of hiatal hernia in relation to the occurrence of eCLE in male and female participants. We also conducted a multivariate binary logistic regression analysis to assess the risk factors for the occurrence of eCLE stratified by sex. To assess associations between categorical data, the χ2 test (or Fisher’s exact test if appropriate) was performed. To compare mean age and body mass index, a t-test or analysis of variance, assuming equal variances, was performed. In all analyses, P<0.05 indicates statistical significance.

Results

Logistic regression analysis of risk factors for eCLE in the UGID study

In this study, 174 cases of eCLE were observed (Table 1). We conducted a multivariable logistic regression analysis to identify the risk factors for eCLE in all 8031 participants (Table 2). Results showed that the independent risk factors of eCLE were presence of hiatal hernia (odds ratio [OR] = 2.89, 95% confidence interval [CI] = 2.12–3.96, P<0.0001), presence of erosive esophagitis (OR = 2.06, 95% CI = 1.48–2.86, P<0.0001), age (10-year increments, OR = 1.44, 95% CI = 1.24–1.68, P<0.0001), and alcohol consumption (≥20 g/day, OR = 1.58, 95% CI = 1.15–2.16, P = 0.005).

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Table 1. Characteristics of participants in UGID study.

https://doi.org/10.1371/journal.pone.0192951.t001

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Table 2. Logistic regression analysis of risk factors for endoscopic columnar-lined esophagus (N = 8031).

https://doi.org/10.1371/journal.pone.0192951.t002

Logistic regression analysis to assess the association between alcohol consumption and the occurrence of eCLE, stratified by hiatal hernia status

To assess the association in the occurrence of eCLE between alcohol intake and hiatal hernia status, we conducted a binary logistic regression analysis (Table 3). To evaluate the effect of a low alcohol consumption, we used three categories of alcohol intake: 0, <20, and ≥20 g/day. Alcohol intake (≥20 g/day) was marginally significantly associated with the incidence of eCLE in participants without hiatal hernia (0 vs. ≥20 g/day; OR, 1.62; 95% CI, 0.92–2.85; P = 0.09) but not in participants with hiatal hernia (0 vs. ≥ 20 g/day; OR, 0.99; 95% CI, 0.59–1.65; P = 0.95). In addition, there was no difference in its relationship with the degree of hiatal hernia (length of the proximal dislocation of the GEJ above the diaphragmatic indentation, 2–4 cm vs. >4 cm; S1 Table).

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Table 3. Logistic regression analysis to assess the association between alcohol consumption and the occurrence of endoscopic columnar-lined esophagus stratified by hiatal hernia status.

https://doi.org/10.1371/journal.pone.0192951.t003

Logistic regression analysis to assess the association between alcohol consumption and the occurrence of eCLE in male and female participants, stratified by hiatal hernia

Next, we conducted a binary logistic regression analysis to assess the association in the occurrence of eCLE between alcohol intake and status of hiatal hernia in male participants (Table 4). Alcohol intake (≥20 g/day) was significantly associated with the incidence of eCLE in male participants without hiatal hernia (0 vs. ≥20 g/day; OR, 1.98; 95% CI, 1.04–4.03; P = 0.04) but not in male participants with hiatal hernia (0 vs. ≥20 g/day; OR, 1.04; 95% CI, 0.60–1.78; P = 0.88). This phenomenon was not observed in female participants (Table 5). Furthermore, there was no significant association between the occurrence of eCLE and alcohol intake simply stratified by sex (S2 Table).

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Table 4. Logistic regression analysis to assess the association between alcohol consumption and the occurrence of endoscopic columnar-lined esophagus, stratified by hiatal hernia in male participants.

https://doi.org/10.1371/journal.pone.0192951.t004

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Table 5. Logistic regression analysis to assess the association between alcohol consumption and the occurrence of endoscopic columnar-lined esophagus stratified by hiatal hernia status in female participants.

https://doi.org/10.1371/journal.pone.0192951.t005

In male participants, hiatal hernia (presence: OR, 2.79; 95% CI, 1.96–4.01; P < 0.0001), age (10-year increments: OR, 1.49; 95% CI, 1.25–1.77; P < 0.0001), and erosive esophagitis (presence: OR, 2.00; 95% CI, 1.38–2.87; P = 0.0002) were risk factors of eCLE in the multivariate logistic regression analysis. Meanwhile, in female participants, only hiatal hernia (presence: OR, 2.88; 95% CI, 1.45–5.56; P = 0.002) was the risk factor of eCLE in the multivariate logistic regression analysis (S3 Table).

Discussion

In this large prospective, multicenter, cohort study, we found a significant association in the occurrence of eCLE between alcohol intake and status of hiatal hernia in male participants. Our data supported the hypothesis that the effect of alcohol consumption might be associated with hiatal hernia status and male sex. With the large number of cases, to the best of our knowledge, this is the first study to examine the association in the occurrence of eCLE among alcohol intake, status of hiatal hernia, and male sex.

Hiatal hernia, GERD (and GERD symptoms), male sex, smoking, and obesity have been reported as risk factors of BE in many previous reports–many of which were in Western countries [12, 15, 25–27]. One recent meta-analysis showed the prevalence and risk factors of BE in Asian countries [28]. In that report, the pooled prevalence of eCLE and histologically confirmed BE was 7.8% and 1.3%, respectively. Most histologically confirmed BEs were cases of SSBE. Reflux symptoms (pooled OR = 3.15, 95% CI = 1.61–6.17), male sex (pooled OR = 1.50, 95% CI = 1.11–2.03), hiatal hernia (pooled OR = 4.88, 95% CI = 2.93–8.13), and smoking (pooled OR = 1.26, 95% CI = 1.01–1.56) were associated with a significantly increased risk of BE. Despite current smoking not having significant association with eCLE, other factors were significantly or likely to be associated with eCLE in our study (Table 2). NERD and the absence of atrophic gastritis were the factors associated with reflux symptoms [29] [30]. These factors were not associated with the incidence of eCLE in this study.

In our study, the effect of alcohol intake on the association between the occurrence of eCLE and hiatal hernia was limited to male participants. Some previous reports suggested that the anti-inflammatory action of estrogen and esophageal epithelial resistance against reflux of gastric acid were associated with the occurrence of BE [17, 18]. In our study, alcohol intake (≥20 g/day) was more frequently observed in male than in female participants, which might be one of the reasons for the high frequency of eCLE in male participants.

The influence of alcohol intake on the incidence of BE has been inconsistent. Alcohol has been shown to reduce LES pressure or lead to a loss of normal regulation of LES function, which, in turn, can lead to increased GERD symptoms [31–33]. Alcohol consumption was reported to lead to a 1.2- to 2.9-fold increased risk of GERD [34, 35]. However, many reports showed a negative association between alcohol consumption and incidence of BE [10, 11]. We hypothesized a significant relationship between alcohol consumption and occurrence of BE in a specific condition. In our study, the effect of alcohol consumption might be limited to male participants without hiatal hernia. This can be attributed to the fact that hiatal hernia can lead to a loss of normal regulation of LES pressure and alcohol consumption does not affect LES pressure in patients with hiatal hernia. Interestingly, the J curve phenomenon was observed in most analyses. Mild alcohol consumption (<20 g/day) would reduce the risk for BE (Tables 3, 4 and 5), whereas a greater alcohol intake (≥20 g/day) might be associated with an increased risk of BE.

Socioeconomic status was also reported as a risk factor for BE [36]. In this study, we did not have access to this information. However, the socioeconomic status of the majority of our participants was likely middle-class because they could pay the relatively expensive health care costs themselves or with support from their employers.

The strength of this study is the use of a large amount of data from a prospective multicenter cohort study. The size and comprehensiveness of this database enabled us to examine the independent association in the occurrence of eCLE among alcohol consumption, male sex, and hiatal hernia, while adjusting for potential confounders. However, our study has some limitations. BE was not confirmed histologically. In Asia, most cases of BE are SSBEs, and it is sometimes difficult to perform multiple biopsies from this region. However, one report showed that the overall prevalence of histological BE in eCLE was 31.7% [28]. Histological confirmation should be included in a future study. Another limitation is the lack of information about alcohol consumption duration (including none) and alcohol type. Additional studies are necessary to clarify whether alcohol consumption or alcohol intake cessation has any additional or independent effect beyond that reported in this study. Thrift AP et al reported that wine was associated with a moderately reduced risk for BE [11]. Therefore, further examination, stratified by alcohol type, is necessary. Moreover, there were no esophageal adenocarcinoma cases associated with BE in this study. The UGID study is a 5-year follow-up study, and additional information will appear in the near future and clarify the effect of alcohol intake on the occurrence of esophageal adenocarcinoma.

In conclusion, the association of alcohol intake with the incidence of eCLE might be related to hiatal hernia status and male sex. To evaluate the effect of alcohol consumption on the incidence of BE, the condition of hiatal hernia and male sex should be considered.

Supporting information

S1 Table. Association between alcohol consumption and the occurrence of an endoscopic columnar-lined esophagus, stratified by hiatal hernia status.

https://doi.org/10.1371/journal.pone.0192951.s001

(DOCX)

S2 Table. Logistic regression analysis to assess the association between alcohol consumption and the occurrence of an endoscopic columnar-lined esophagus, stratified by sex.

https://doi.org/10.1371/journal.pone.0192951.s002

(DOCX)

S3 Table. Logistic regression analysis of risk factors for endoscopic columnar-lined esophagus, stratified by sex.

https://doi.org/10.1371/journal.pone.0192951.s003

(DOCX)

S1 File. Upper gastrointestinal disease study questionnaire.

https://doi.org/10.1371/journal.pone.0192951.s004

(DOCX)

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