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Intra-venous bevacizumab in hereditary hemorrhagic telangiectasia (HHT): A retrospective study of 46 patients

  • Alexandre Guilhem ,

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Validation, Writing – original draft, Writing – review & editing

    Affiliation Médecine Interne - Maladies Multi-Organiques, Hôpital Saint Eloi, Montpellier, France

  • Anne-Emmanuelle Fargeton,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Génétique - Centre de Référence National Maladie de Rendu-Osler, Hôpital Femme-Mère-Enfant, Bron, France

  • Anne-Claire Simon,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Pneumologie, CHU de Poitiers, Poitiers, France

  • Pierre Duffau,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Médecine Interne et Immunologie Clinique, Hôpital Saint-André, Bordeaux, France

  • Jean-Robert Harle,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Département de Médecine Interne, Hôpital de la Timone, Marseille, France

  • Christian Lavigne,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Médecine Interne et Maladies Vasculaires, CHU d’Angers, Angers, France

  • Marie-France Carette,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Radiologie, Hôpital Tenon, Paris, France

  • Olivier Bletry,

    Roles Writing – review & editing

    Affiliation Service de Médecine Interne, Hôpital Foch, Suresnes, France

  • Pierre Kaminsky †,

    † Deceased.

    Roles Conceptualization, Data curation, Investigation, Resources

    Affiliation Service de Médecine Interne, Hôpital de Brabois, Vandoeuvre-lès-Nancy, France

  • Vanessa Leguy,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Médecine Interne et Immunologie Clinique, Hôpital François Mitterrand, Dijon, France

  • Nathalie Lerolle,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Médecine Interne, Hôpital Bicêtre, Paris, France

  • Dominique Roux,

    Roles Investigation, Writing – review & editing

    Affiliation Service de Médecine, Centre hospitalier, Ambert, France

  • Marc Lambert,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Médecine Interne, Hôpital Huriez, Lille, France

  • Thierry Chinet,

    Roles Data curation, Investigation, Resources, Supervision, Writing – review & editing

    Affiliation Service de Pneumologie, Hôpital Ambroise Paré, Boulogne-Billancourt, France

  • Delphine Bonnet,

    Roles Data curation, Investigation, Resources, Writing – review & editing

    Affiliation Service de Médecine Interne du Pôle digestif, CHU Purpan, Toulouse, France

  • Sophie Dupuis-Girod,

    Roles Conceptualization, Data curation, Investigation, Resources, Validation, Writing – original draft, Writing – review & editing

    Affiliation Service de Génétique - Centre de Référence National Maladie de Rendu-Osler, Hôpital Femme-Mère-Enfant, Bron, France

  •  [ ... ],
  • Sophie Rivière

    Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Writing – original draft, Writing – review & editing

    Affiliation Médecine Interne - Maladies Multi-Organiques, Hôpital Saint Eloi, Montpellier, France

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Intra-venous bevacizumab in hereditary hemorrhagic telangiectasia (HHT): A retrospective study of 46 patients

  • Alexandre Guilhem, 
  • Anne-Emmanuelle Fargeton, 
  • Anne-Claire Simon, 
  • Pierre Duffau, 
  • Jean-Robert Harle, 
  • Christian Lavigne, 
  • Marie-France Carette, 
  • Olivier Bletry, 
  • Pierre Kaminsky, 
  • Vanessa Leguy



Bevacizumab, an anti-VEGF monoclonal antibody, has recently emerged as a new option for severe forms of hereditary hemorrhagic telangiectasia (HHT). Its utilization in this orphan disease has rapidly spread despite the lack of randomized trials and international guidelines. The objective of this study is to report the main clinical data (baseline characteristics, dose schedule, efficacy, adverse events and deaths) of HHT patients treated by intravenous bevacizumab in France.


Retrospective observational study of HHT patients treated with bevacizumab for a severe form of the disease in the 14 centers of the French HHT network.


Forty-six patients (median age: 68 years) were treated between March 2009 and May 2015. Ten patients were treated for high output cardiac failure, 20 patients for severe hemorrhages and 16 for both indications. The standard protocol (6 infusions of 5mg/kg every 2 weeks) was initially used in 89% of the cases but diverse strategies were subsequently applied. A clinical improvement was noted by the referent physician for 74% of the patients with a median effect’s duration of 6 months. Wound healing complications led to 2 amputations. Arthralgia/arthritis and arterial hypertension occurred in 5 patients each. One third of the patients were dead at the time of the final update, coherently with age and the poor prognosis of these highly symptomatic patients.


Intravenous bevacizumab seems to provide a clinical benefice in severe HHT patients. Precautions concerning wound healing and vascular pathologies must be respected. Prospective double blinded versus placebo trials are needed.


Hereditary Hemorrhagic Telangiectasia (HHT) is a worldwide vascular genopathy with an incidence rate estimated from 1/5000 to 1/8000. This autosomic dominant disorder is caused by the haplo-insufficiency of at least 3 genes (ENG, ALK1 and SMAD4) belonging to the BMP/SMAD signaling pathway. Data suggest that it results in an imbalanced state between pro- and anti-angiogenic factors, including a high level of Vascular Endothelial Growth Factor (VEGF), leading to dysregulation of the neo-angiogenesis [1]. The main clinical features are telangiectasia of the face, the fingers and the nasal mucosa, which are often complicated by recurrent epistaxis and anemia. Telangiectasia less frequently affect the gastro-intestinal (GI) tract and can cause occult bleedings. Larger arterio-venous malformations (AVMs) can occur, affecting the lung (with a risk of stroke or cerebral abscess), the liver (with a risk of high-output cardiac failure (HCF)), or the brain (with a risk of intracranial hemorrhage) [2].

Bevacizumab is an anti-VEGF monoclonal humanized antibody designed to inhibit the tumor induced neo-angiogenesis. Its clinical use is authorized since 2005 as an adjuvant treatment for many kinds of metastatic or locally advanced solid cancers [3]. In 2006 then 2008, its administration to HHT patients with a concurrent oncologic condition has been associated to a dramatic improvement of epistaxis and HCF [4,5]. Therefore, an open label phase 2 single arm trial has been conducted in France in 2009 in 25 patients with a symptomatic liver involvement. A significant decrease of high cardiac output and epistaxis duration has been demonstrated [6]. Since this date, bevacizumab has been used to treat severe HHT-related symptoms.

The aim of our study is to describe the use of bevacizumab through the French HHT network with particular considerations for efficacy, adverse effects and death causes in this specific orphan disease.

Patients and methods

Data collection

Cases were defined as adult patients with definite HHT diagnosis according to the Curaçao criteria and having already received at least one intra-venous injection of bevacizumab. Regular calls for cases have been made in the centers of the French HHT network between March 1st, 2009 and May 30th, 2015. A standardized form was sent to the physician in charge of the patient as soon as the case was notified. This form was designed to anonymously collect data on the indication of bevacizumab, demographic parameters, HHT diagnosis criteria and related symptoms, concomitant conditions and treatment, including dose schedule, adverse events and global efficacy.

Each case was classified in a group according to the therapeutic goal: attenuating the high-output heart failure due to hepatic AVM (HCF group), reducing the transfusion needs or the number of life-threatening hemorrhages by epistaxis or GI bleeding (Severe Hemorrhage (SH) group) or both (HCF+SH group). Standard protocol was considered as 6 administrations of 5 mg/kg of bevacizumab, each spaced by 14 days. Re-treatment’s procedure was defined by a new administration sequence in case of symptom recurrence after the end of the initial treatment. Maintenance therapy was defined by systematic and planned administrations after the initial treatment, independently from the intensity of HHT-related symptoms. To objectively assess the treatment efficacy, physicians were requested to detail, if available, NYHA class of dyspnea, cardiac index, hemoglobin level, transfusion frequency and epistaxis duration, before and after treatment. A global judgment of the clinical efficacy (improvement, stability, aggravation) was also systematically asked to the physician after having reviewed their files with patient’s statements, physical signs, laboratory results, imaging and associated treatment. A minimum period of three months after the first injection was required to establish this assessment. All clinically relevant events occurring after the first injection were considered as adverse events and registered, whatever the time interval between the treatment and the event. Exceptions were made for GI bleeding and epistaxis, considered as usual HHT hemorrhagic events.

Once completed and returned, all the forms were reviewed by AG or SR. Complementary data were directly asked to the physician for clarification if required. In case of death, the main cause and the associated circumstances were discussed between the referent physician and AG or SR.

The current status (ongoing treatment, adverse event, death) of all included patients was updated between July and August, 2015.

Statistical analysis

Patients and treatments characteristics are presented in tables. Quantitative variables are described with median, minimum and maximum values. Categorical variables are given in absolute number and percentage. Statistical comparisons between HCF, SH and HCF+SH groups were judged non pertinent given the objective and the size of the study. Potential predicting factors of efficacy were assessed by univariate models of logistic regression.

A specific R script was built to generate all the statistical data (R version 3.1.2 (2014-10-31)) [7]. This script is fully available at A p-value below 0.05 was considered as significant.

Ethics statement

Patients having medical care through the French HHT network give a written consent for epidemiological studies prior to be included in the national HHT database (CIROCO). This computerized database is registered and approved by the French National Committee for Information Technology and Freedom (CNIL).

All patients were informed by their referent physician of the benefits and the risks potentially associated with bevacizumab and gave informed consent. In accordance to the French law (Loi française n° 2004–806 du 9 août 2004 relative à la politique de santé publique et ses décrets d’application), specific authorization by an ethic committee is not mandatory for a retrospective non-interventional protocol.


Patient’s characteristics

Forty-six patients received intra-venous bevacizumab between March 2009 and May 2015. All of them were included in the study and analyzed. The median duration of the treatment was 106 days, corresponding to a total exposition of 51 patient-years.

With a median age of 68 years and a well-balanced sex ratio (48% male), the cohort was characterized by a large majority of mutated ALK1 carriers (80%), with hepatic (89%) and digestive (63%) involvements. One patient had received a hepatic transplant for HHT-related disease 15 years before. The most common comorbidities were atrial fibrillation (24%) and arterial hypertension (22%). About one quarter (24%) of the patients had previously experienced at least one venous thrombo-embolic event and a similar proportion had a history of severe sepsis (Table 1).

Table 1. Patient’s characteristics before treatment (n = 46).

Bevacizumab use

Table 2 describes bevacizumab use. Among the 10 patients (22%) treated only for symptomatic high output cardiac failure (HCF), 8 (80%) had a class III or IV NYHA dyspnea at the beginning of the treatment. The median cardiac index was 5.2 l/min/m2, after exclusion of patients with atrial fibrillation (cardiac index is not reliable in this situation). Twenty patients (44%) who were treated exclusively for severe hemorrhages were receiving a median of 3 packed red blood cells (PRBC) per month before treatment. One of them was affected by rare but massive and life threatening epistaxis with subnormal hemoglobin level between the episodes (113 g/l just before the treatment’s start). Sixteen patients (35%) were treated concomitantly for both indications.

Table 2. Initial severity of disease, modalities of use, efficacy and safety of bevacizumab.

The standard protocol was the most often used (89%) for initial treatment. A treatment continuation was decided for about the half of the cohort (54%) (re-treatment or maintenance therapy). Twelve patients were treated for more than one year. Four patients received bevacizumab injections during more than 4 years.

Three patients underwent orthotopic liver transplantation after the bevacizumab treatment. They were 46, 53 and 63 years old, all treated for HCF and considered as improved by bevacizumab. One of them had experienced an adverse event (haemoperitoneum) leading to the discontinuation of the treatment. The time between the last injection of bevacizumab and the surgical procedure was 11, 15 and 17 months. No specific concerns were raised after the transplant procedure, especially concerning wound healing.

Objective measures of efficacy after treatment (NYHA class of dyspnea, cardiac index, hemoglobin level, transfusion frequency and epistaxis duration) were very heterogeneous in terms of modalities and timing of evaluation. In some cases, data were missing. For these reasons, we were not able to adequately perform statistical analysis of the objective efficacy parameters. The clinical appreciation of efficacy by the referent physician was obtained for all cases. Improvement of the HHT-related symptoms was noted in 74% of the whole cohort. The median duration of efficacy was 6 months (minimum: 1 month, maximum: 20 months). In univariate, no patient characteristics were found to be significantly associated with bevacizumab efficacy. No improvement was observed in both cases of a twin’s pair, treated at an interval of 15 months.

The most frequent adverse event was infections (22%) including 3 pneumonias, 1 bacteremia, 1 osteitis, 1 urinary tract infection, 1 epididymitis, 1 ophthalmic infection, 1 herpes zoster, 1 sepsis of undetermined origin. Arterial hypertension and rheumatologic symptoms were less frequent (11% each). Wound-healing complications occurred in 3 patients, 2 of them underwent leg amputation and the third developed of severe bedsores. Loss of efficacy under prolonged therapy (maintenance or re-treatment) was reported for 2 patients but no exacerbation of HHT-related symptoms was observed after treatment cessation. No case of proteinuria was notified. Fifteen (33%) patients died during the follow-up.

Details on the death cases having occurred during the follow-up are reported in S1 Table. Seven deaths were related to heart failure, 4 to sepsis and 2 to massive hemorrhages. Three deaths (2 sepsis, 1 ischemic cholangitis) occurred within 6 months after the last injection. Most of these 15 patients were considered as bevacizumab non-responders or had stopped it after an adverse event.



With an improvement of 74% of the whole cohort, our study strongly suggests a clinical benefit of bevacizumab for HHT patients. This result is only based on physicians’ judgments since the objective parameters of efficacy were unfortunately not exploitable (different timing and modalities of evaluation, missing data). The overall assessment of an expert physician is considered as a reliable and relevant scoring system in many complex diseases. By example, the PGA (physician’s global assessment) in lupus and the IGA (Investigator global assessment) in atopic dermatitis are well-recognized scores based on this principle [810].

Bevacizumab seems to be as effective in severe hemorrhage as in HCF. This is consistent with the central role of the VEGF dysregulation in HHT [11].

Bevacizumab appears to have an only suspensive effect. All patients have experienced symptoms recurrence after the discontinuation of the treatment, but after a large range of time (1 to 20 months). The same phenomenon has recently been reported in the long term study of the patients included in the French open label trial [12] and is coherent with the pharmacological effects of the drug [13]. Prolonged therapy (re-treatment or maintenance) was more frequent in the SH group (70% vs 20% and 56% for HCF and HCF+SH), potentially because of a clinically more obvious benefit. Loss of efficacy is rare, and no rebound effect after treatment cessation has been observed.

We observed that about one quarter of the patients did not exhibit a clinical improvement. This is higher than the 17% reported by Dupuis-Girod et al. in a prospective trial [6] but our cohort is composed of non-selected patients with concomitant conditions encountered in the daily practice. No patient’s characteristic at the baseline seems to be predictive to a bevacizumab response. The lack of bevacizumab efficacy in the twin’s pair argues for a role of the genetic background. Polymorphisms and genetic modifiers are likely to explain the high variability of HHT expression among carriers of the same mutation [14,15]. They could also modulate the patient’s sensibility to bevacizumab. Further studies on larger populations should be conducted to identify such predictive factors and guide therapeutic choices. Until then, the only possibility is to initiate treatment for each patient in order to test his individual responsiveness.

Orthotopic liver transplant remains the only curative option in case of severe hepatic involvement. Three of our patients were treated by bevacizumab prior to the transplant procedure. No specific problems were reported but a long delay between the last injection and the surgery was respected. Bevacizumab seems to be able to improve the pre-transplant clinical status and could be a valuable option for frail candidates to surgery. It also involves a risk of missing the “transplant window” for patients under 65 years and the decision to treat with bevacizumab must be carefully considered in this context [16].

The study was not designed to properly analyze the medico-economic impact of bevacizumab. Nevertheless, for the most highly responsive patients of the cohort, this treatment is an obvious cost-effective option given the reduction of overall hospital care costs related to acute hemorrhagic events and heart failure. This economic benefit has also been suggested by others studies [17,18] and is an important point to assess in the future.

Adverse events

One important contribution of our study is to provide a global view of the adverse events associated with bevacizumab in this chronic and non-oncologic context.

Blood pressure elevation has concerned 5 patients (11%). It is an expected side effect of bevacizumab and can be most of time easily managed [19]. Arthralgia or arthritis has been reported in 5 (11%) cases here, although it is not a well-known side-effect of bevacizumab. It could correspond to an attenuated form of serum sickness related to immune complexes including bevacizumab as described with other monoclonal antibodies [20]. Other mechanisms could be hypothesized, like hypoxia induced by synovial inflammation due the anti-VEGF effect [21,22] or a complication of severe hypophosphatemia related to intravenous iron administration [23], frequently used in this population. Next cases should be more precisely documented to better understand the underlying physiopathology. The high frequency of infections and heart failure are more probably related to the natural evolution of HHT [24,25] but have also been described with bevacizumab [26,27]. No case of venous thromboembolism has been diagnosed during the study, but it has been recently reported by Maestraggi [28]. Surprisingly, no proteinuria has been reported while it is one of the major concern for type 2 neurofibromatosis’s patients treated by bevacizumab [29,30]. This adverse effect is reported with a median time under treatment longer than 2 years, which is the case for only 6 patients in the present study. It should regularly be checked, especially in case of long term treatment.

Among the various adverse events reported here, the wound healing complications were highly problematic and have led to two cases of lower limb amputation. This process appears to be importantly disturbed by bevacizumab and special attention to skin wound and surgery should be kept in mind. In the oncologic context, it is recommended to stop bevacizumab 6 to 8 weeks before the surgery and to restart it at least 4 weeks after and only if the wound is totally healed [31]. In the same way, unstable or severe arteriopathy as well as recent thrombotic events are highly risked situations and should reasonably lead to delay or refuse this treatment [3234].

In this cohort, 33% of patients died during the follow up, essentially from usual HHT complications: end-stage heart failure, sepsis and cataclysmic hemorrhage. It has occurred either by unresponsive patients or after a long time after treatment cessation (up to 55 months). Mortality studies in HHT patients are scarce. In UK, diagnosis of HHT is associated with a 3-years reduction in median age at death [35]. In contrast, Kjeldsen et al [36] have shown a high mortality rate (20 of 28 patients on a 21 years’ period) in a group of patients with severe HHT-related symptoms characterized by “daily or weekly episodes of severe bleeding leading to severe anemia and transfusions, hospitalization”. Most of our patients could have been classified in this group and this severe prognosis constitutes an obvious recruitment bias explaining the observed mortality rate. These observations argue for proposing bevacizumab earlier in the disease’s natural history, to less frail subjects. Two treatment thresholds could be considered: a prolonged state of transfusion dependency or the persistence of heart failure symptoms after cardiologic treatment optimization.

Limitations of the study

Our work suffers from limitations related to its retrospective, non-interventional and open design. The retrospective data collection and the diversity of participating centers have not allowed us to obtain standardized objective parameters for the efficacy assessment. Nevertheless, we think that our data honestly reflect the daily practice and provide valuable information to apprehend the risk/benefit ratio of this new drug. It constitutes a preliminary step to conducting a large, prospective, and randomized versus placebo trial, which is deeply needed.

The lack of consensual modalities of bevacizumab use, especially for long term treatments, is another concern. It has generated a high level of heterogeneity in our data. Most of physicians have initially followed the "standard protocol" of 6 injections of 5mg/kg, by analogy with the oncological use and the prospective trial [6]. Other protocols with lower doses have also been proposed [37,38] and could be more appropriate in some cases, notably for frail patients. Concerning the subsequent treatment (maintenance or retreatment), a pharmacological study based on a mathematical model suggested a systematic monthly injection [39]. A retreatment strategy individually adapted to the clinical needs has been applied for 24% of the cohort. This strategy could be pertinent but requires to be sustained by further clinical and biopharmaceutical investigations.


In the field of rare diseases, therapeutic innovations are rare and challenging events. This multicenter retrospective study of 46 patients is, to our knowledge, the most important retrospective cohort of HHT patients treated by intra-venous bevacizumab. It supports a clinically pertinent therapeutic effect for highly symptomatic patients. Wound’s healing complications are a major limitation and should be considered as a contraindication. This collective experience leads us to provide some basic guidelines for the French “temporary use recommendations” of bevacizumab for HHT patients, summarized in Fig 1. A prospective double blinded versus placebo study for intra-venous bevacizumab in HHT is needed.

Fig 1. Recommendations for the use of intra-venous bevacizumab in HHT patients.

Supporting information

S1 Table. Characteristics of the cohort’s patients who died during the follow up.



All the authors are very grateful to Arthur Lucchino for his assistance concerning the statistical analysis and to Yohan Audurier for his help in data collection.


  1. 1. Ardelean DS, Letarte M. Anti-angiogenic therapeutic strategies in hereditary hemorrhagic telangiectasia. Front Genet. 2015;6: 35. pmid:25717337
  2. 2. Bailly S, Dupuis-Girod S, Plauchu H. [Rendu-Osler disease: clinical and molecular update]. Médecine Sci MS. 2010;26: 855–860. pmid:20929677
  3. 3. Amit L, Ben-Aharon I, Vidal L, Leibovici L, Stemmer S. The Impact of Bevacizumab (Avastin) on Survival in Metastatic Solid Tumors—A Meta-Analysis and Systematic Review. PLoS ONE. 2013;8. pmid:23349675
  4. 4. Flieger D, Hainke S, Fischbach W. Dramatic improvement in hereditary hemorrhagic telangiectasia after treatment with the vascular endothelial growth factor (VEGF) antagonist bevacizumab. Ann Hematol. 2006;85: 631–632. pmid:16807748
  5. 5. Mitchell A, Adams LA, MacQuillan G, Tibballs J, vanden Driesen R, Delriviere L. Bevacizumab reverses need for liver transplantation in hereditary hemorrhagic telangiectasia. Liver Transplant Off Publ Am Assoc Study Liver Dis Int Liver Transplant Soc. 2008;14: 210–213. pmid:18236396
  6. 6. Dupuis-Girod S, Ginon I, Saurin J-C, Marion D, Guillot E, Decullier E, et al. Bevacizumab in patients with hereditary hemorrhagic telangiectasia and severe hepatic vascular malformations and high cardiac output. JAMA. 2012;307: 948–955. pmid:22396517
  7. 7. R: The R Project for Statistical Computing [Internet]. [cited 10 Sep 2017].
  8. 8. Petri M, Hellmann D, Hochberg M. Validity and reliability of lupus activity measures in the routine clinic setting. J Rheumatol. 1992;19: 53–59. pmid:1556700
  9. 9. Luijten KMAC, Tekstra J, Bijlsma JWJ, Bijl M. The Systemic Lupus Erythematosus Responder Index (SRI); a new SLE disease activity assessment. Autoimmun Rev. 2012;11: 326–329. pmid:21958603
  10. 10. Rehal B, Armstrong AW, Armstrong A. Health outcome measures in atopic dermatitis: a systematic review of trends in disease severity and quality-of-life instruments 1985–2010. PloS One. 2011;6: e17520. pmid:21533286
  11. 11. Sadick H, Riedel F, Naim R, Goessler U, Hörmann K, Hafner M, et al. Patients with hereditary hemorrhagic telangiectasia have increased plasma levels of vascular endothelial growth factor and transforming growth factor-beta1 as well as high ALK1 tissue expression. Haematologica. 2005;90: 818–828. pmid:15951295
  12. 12. Dupuis-Girod S, Ginon I, Saurin JC, Marion D, Guillot E, Decullier E,et al. Metafore study: 8 years later. Follow-up of HHT patients treated with bevacizumab for severe hepatic vascular malformations and high cardiac output. 12th HHT international conference, Croatia; 2017.
  13. 13. Ranieri G, Patruno R, Ruggieri E, Montemurro S, Valerio P, Ribatti D. Vascular endothelial growth factor (VEGF) as a target of bevacizumab in cancer: from the biology to the clinic. Curr Med Chem. 2006;13: 1845–1857. pmid:16842197
  14. 14. Pawlikowska L, Nelson J, Guo DE, McCulloch CE, Lawton MT, Young WL, et al. The ACVRL1 c.314-35A>G polymorphism is associated with organ vascular malformations in hereditary hemorrhagic telangiectasia patients with ENG mutations, but not in patients with ACVRL1 mutations. Am J Med Genet A. 2015;167: 1262–1267. pmid:25847705
  15. 15. Letteboer TGW, Benzinou M, Merrick CB, Quigley DA, Zhau K, Kim I-J, et al. Genetic variation in the functional ENG allele inherited from the non-affected parent associates with presence of pulmonary arteriovenous malformation in hereditary hemorrhagic telangiectasia 1 (HHT1) and may influence expression of PTPN14. Front Genet. 2015;6: 67. pmid:25815003
  16. 16. Dupuis-Girod S, Buscarini E. Hereditary hemorrhagic telangiectasia: to transplant or not to transplant? Liver Int Off J Int Assoc Study Liver. 2016;36: 1741–1744. pmid:27864870
  17. 17. Arizmendez NP, Rudmik L, Poetker DM. Intravenous bevacizumab for complications of hereditary hemorrhagic telangiectasia: a review of the literature. Int Forum Allergy Rhinol. 2015;5: 1042–1047. pmid:26202958
  18. 18. Ou G, Galorport C, Enns R. Bevacizumab and gastrointestinal bleeding in hereditary hemorrhagic telangiectasia. World J Gastrointest Surg. 2016;8: 792–795. pmid:28070235
  19. 19. Mir O, Coriat R, Ropert S, Cabanes L, Blanchet B, Camps S, et al. Treatment of bevacizumab-induced hypertension by amlodipine. Invest New Drugs. 2012;30: 702–707. pmid:20878444
  20. 20. Ungprasert P, Srivali N, Kittanamongkolchai W, Leeaphorn N, Griger DT. Rituximab-induced serum sickness in overlapping syndrome between sjögren syndrome and systemic lupus erythematosus. J Clin Rheumatol Pract Rep Rheum Musculoskelet Dis. 2013;19: 360. pmid:23965477
  21. 21. Konisti S, Kiriakidis S, Paleolog EM. Hypoxia—a key regulator of angiogenesis and inflammation in rheumatoid arthritis. Nat Rev Rheumatol. 2012;8: 153–162. pmid:22293762
  22. 22. Ng CT, Biniecka M, Kennedy A, McCormick J, Fitzgerald O, Bresnihan B, et al. Synovial tissue hypoxia and inflammation in vivo. Ann Rheum Dis. 2010;69: 1389–1395. pmid:20439288
  23. 23. Blazevic A, Hunze J, Boots JMM. Severe hypophosphataemia after intravenous iron administration. Neth J Med. 2014;72: 49–53. pmid:24457442
  24. 24. Garg N, Khunger M, Gupta A, Kumar N. Optimal management of hereditary hemorrhagic telangiectasia. J Blood Med. 2014;5: 191–206. pmid:25342923
  25. 25. Dupuis-Girod S, Giraud S, Decullier E, Lesca G, Cottin V, Faure F, et al. Hemorrhagic hereditary telangiectasia (Rendu-Osler disease) and infectious diseases: an underestimated association. Clin Infect Dis Off Publ Infect Dis Soc Am. 2007;44: 841–845. pmid:17304458
  26. 26. Qi W-X, Fu S, Zhang Q, Guo X-M. Bevacizumab increases the risk of severe congestive heart failure in cancer patients: an up-to-date meta-analysis with a focus on different subgroups. Clin Drug Investig. 2014;34: 681–690. pmid:25096848
  27. 27. Qi W-X, Fu S, Zhang Q, Guo X-M. Bevacizumab increases the risk of infections in cancer patients: A systematic review and pooled analysis of 41 randomized controlled trials. Crit Rev Oncol Hematol. 2015;94: 323–336. pmid:25749417
  28. 28. Maestraggi Q, Bouattour M, Toquet S, Jaussaud R, Kianmanesh R, Durand F, et al. Bevacizumab to Treat Cholangiopathy in Hereditary Hemorrhagic Telangiectasia: Be Cautious: A Case Report. Medicine (Baltimore). 2015;94: e1966. pmid:26579805
  29. 29. Fangusaro J, Gururangan S, Poussaint TY, McLendon RE, Onar-Thomas A, Warren KE, et al. Bevacizumab (BVZ)-associated toxicities in children with recurrent central nervous system tumors treated with BVZ and irinotecan (CPT-11): a Pediatric Brain Tumor Consortium Study (PBTC-022). Cancer. 2013;119: 4180–4187. pmid:24104527
  30. 30. Slusarz KM, Merker VL, Muzikansky A, Francis SA, Plotkin SR. Long-term toxicity of bevacizumab therapy in neurofibromatosis 2 patients. Cancer Chemother Pharmacol. 2014;73: 1197–1204. pmid:24710627
  31. 31. Gordon CR, Rojavin Y, Patel M, Zins JE, Grana G, Kann B, et al. A review on bevacizumab and surgical wound healing: an important warning to all surgeons. Ann Plast Surg. 2009;62: 707–709. pmid:19461291
  32. 32. Schutz F a. B, Je Y, Azzi GR, Nguyen PL, Choueiri TK. Bevacizumab increases the risk of arterial ischemia: a large study in cancer patients with a focus on different subgroup outcomes. Ann Oncol Off J Eur Soc Med Oncol ESMO. 2011;22: 1404–1412. pmid:21115602
  33. 33. Zuo P-Y, Chen X-L, Liu Y-W, Xiao C-L, Liu C-Y. Increased Risk of Cerebrovascular Events in Patients with Cancer Treated with Bevacizumab: A Meta-Analysis. PLoS ONE. 2014;9. pmid:25025282
  34. 34. Chen X-L, Lei Y-H, Liu C-F, Yang Q-F, Zuo P-Y, Liu C-Y, et al. Angiogenesis Inhibitor Bevacizumab Increases the Risk of Ischemic Heart Disease Associated with Chemotherapy: A Meta-Analysis. PLoS ONE. 2013;8. pmid:23818962
  35. 35. Donaldson JW, McKeever TM, Hall IP, Hubbard RB, Fogarty AW. Complications and mortality in hereditary hemorrhagic telangiectasia: A population-based study. Neurology. 2015;84: 1886–1893. pmid:25862798
  36. 36. Kjeldsen AD, Vase P, Green A. Hereditary haemorrhagic telangiectasia: a population-based study of prevalence and mortality in Danish patients. J Intern Med. 1999;245: 31–39. pmid:10095814
  37. 37. Thompson AB, Ross DA, Berard P, Figueroa-Bodine J, Livada N, Richer SL. Very low dose bevacizumab for the treatment of epistaxis in patients with hereditary hemorrhagic telangiectasia. Allergy Rhinol Provid RI. 2014;5: 91–95. pmid:25199101
  38. 38. Wee JW, Jeon YW, Eun JY, Kim HJ, Bae SB, Lee KT. Hereditary hemorrhagic telangiectasia treated with low dose intravenous bevacizumab. Blood Res. 2014;49: 192–195. pmid:25325040
  39. 39. Azzopardi N, Dupuis-Girod S, Ternant D, Fargeton A-E, Ginon I, Faure F, et al. Dose—response relationship of bevacizumab in hereditary hemorrhagic telangiectasia. mAbs. 2015;7: 630–637. pmid:25751241